LOCUS       NZ_AP022935             3940 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J7 plasmid pJ7_10, complete sequence.
ACCESSION   NZ_AP022935
VERSION     NZ_AP022935.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203332
            Sequence Read Archive: DRR206372, DRR206396
            Assembly: GCF_021655135.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 344x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655135.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:11:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,087
            CDSs (total)                      :: 5,938
            Genes (coding)                    :: 5,644
            CDSs (with protein)               :: 5,644
            Genes (RNA)                       :: 149
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 294
            CDSs (without protein)            :: 294
            Pseudo Genes (ambiguous residues) :: 0 of 294
            Pseudo Genes (frameshifted)       :: 177 of 294
            Pseudo Genes (incomplete)         :: 134 of 294
            Pseudo Genes (internal stop)      :: 43 of 294
            Pseudo Genes (multiple problems)  :: 50 of 294
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022935.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J7"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ7_10"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            complement(join(3376..3940,1..125))
                     /locus_tag="L6482_RS27685"
     CDS             complement(join(3376..3940,1..125))
                     /locus_tag="L6482_RS27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002205120.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236801726.1"
                     /translation="MSQLNEKRKKEREDAHNEKKAKFEALPNGVKAISKALNKASKKAH
                     NKDLEIEKSKQVLTDEEMEKACELQSKAKAQGMVLLPERRVKNRARFVQIMQDNWSFIQ
                     GHQYLTSEQIVFLMSLVPHVAIGSNAIVDVPRKKQPVGLNQSEIAAVLGTSKAKVSRVV
                     KSLVEKGVLAKAESGLEGNNVKSYVIFVNPHILISGSKDSVEDSLKIMFAKAMKMPVLK
                     NLPERLF"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(727..963)
                     /locus_tag="L6482_RS27690"
                     /old_locus_tag="BCJMU07_p101"
     CDS             complement(727..963)
                     /locus_tag="L6482_RS27690"
                     /old_locus_tag="BCJMU07_p101"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236801729.1"
                     /translation="MTRYPVFQEYQCTYPDPLLEFDIDWWTVSELKELFDITLTDKEKE
                     TRIQAFKTAQGYRGYAAVYSKKEILDLRLVINE"
                     /name="hypothetical protein"
     gene            complement(992..1258)
                     /locus_tag="L6482_RS27695"
                     /old_locus_tag="BCJMU07_p102"
     CDS             complement(992..1258)
                     /locus_tag="L6482_RS27695"
                     /old_locus_tag="BCJMU07_p102"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236801728.1"
                     /translation="MKFTINKKFILAKVEEKEKINLYTLVDNDNYEKITTIGVKPELKI
                     EERSLVEAEVSIRTQSERFELKNNEKKYVEVASFFVSKIQKVK"
                     /name="hypothetical protein"
     gene            complement(1508..1909)
                     /locus_tag="L6482_RS27700"
                     /old_locus_tag="BCJMU07_p103"
     CDS             complement(1508..1909)
                     /locus_tag="L6482_RS27700"
                     /old_locus_tag="BCJMU07_p103"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236801727.1"
                     /translation="MNKIAKEHLTIGEKPAREALKKAGCYATSGKKGWFFDGDAEILEK
                     SIYDFATVKATANPRTKKQISTKTKDVNNKGDEEPMNGTTNDVKNKRKRASFDIDVDLL
                     KQLKVKAVLEEKNLYDLVEQAIKDFLNKK"
                     /name="hypothetical protein"
ORIGIN
        1 ttagaagctt tgttcaatgc tttactaatt gccttaacac catttggaag tgcttcaaac
       61 ttagcttttt tttcattatg agcgtcttca cgctcttttt tacgtttttc attcaactga
      121 ctcatatatg tattcccctc tcgttttaaa aaaagagaaa agaaggtaaa cgtacacttt
      181 ttgaactaca caaaaatatg ataatatatt atacttattt tacatgacta agtactattt
      241 gtactgttta cacaaagctc tcaagtgacc gcttgagagc tttttctttt ctcttgaatt
      301 ttagcaatct aagattcttt ctgcaagcct caccaggctt cctattaatt cgatagtctt
      361 cttgttaatt cgatatatat cttttaacct ctcaaaacga ttctaagacg ctttttgacg
      421 agaaactaca tgtcttccct agatttttca caaaggtacc ttaaaatcga ttctagagct
      481 aatcacaatt ttatttttca taaaaatctt cgattcccat ttttataaag aaaaaccacg
      541 aaattaatgc tacaaatttt tttacaatcc attctagcat ctagatccac ctcactttct
      601 ttgtcttcgc ttccgaacca aggacggaag ttcacttatt cgcgaggctg cgttcgttcc
      661 gtgcttggtt cgaaaagcga aatgacaaag aataaaaaac taggctacga caagctacgc
      721 gctacactac tcatttataa ctaatctcaa atccaatatt tctttcttag aatacacagc
      781 tgcgtaccct ctgtaacctt gcgccgtctt aaatgcttgt attctagttt ctttctcttt
      841 atcggtaagc gttatatcaa aaagttcttt aagctcgctt acggtccacc aatcgatatc
      901 aaattctagt aaaggatctg gatacgtgca ttgatattct tgaaataccg gatacctagt
      961 caattctact tgttcttttg tatacataga tttacttcac tttctgaatc ttagatacga
     1021 aaaatgacgc aacttccaca tattttttct cgttattttt taattcaaaa cgctcgcttt
     1081 gtgtacgaat acttacttca gcttcaacaa gtgaacgttc ttcaattttt aattcaggtt
     1141 taaccccaat cgttgtaatt ttttcataat tgtcattgtc cactaacgta tataaattaa
     1201 ttttttcttt ttcctcaacc ttagctaaaa taaatttttt attaatcgta aatttcatat
     1261 tttcatcccc ttttatattt aaaatttcat gtattgtaac ctccaatttc attatgtcaa
     1321 actttattaa cacctttttt aattacactt ttttaatccc ccaaaccccc aaagggggcc
     1381 ctctcctcgc cggatggcgc cttccaaaca ttgtttggaa gacaacaaga ggatttttag
     1441 tgtagtgcca tatcaacttc ttatcctcct ttgtccgtcg gacacgtccg gtaccgttga
     1501 catgaatcta ttttttgttt aaaaaatctt taatagcttg ttccactaaa tcgtataagt
     1561 tcttttcttc caatactgct ttaactttca attgttttag caaatccaca tcgatatcaa
     1621 aagacgctct ctttcttttg ttctttacat cattagttgt tccgttcata ggttcttcat
     1681 ctcctttatt gttaacatct ttagtttttg tgcttatttg ttttttagtt cttgggttcg
     1741 ccgtagcttt taccgttgca aaatcataaa tacttttttc taaaatctct gcatctccat
     1801 caaaaaacca accttttttg cctgaagttg cataacaacc agccttttta agcgcttcac
     1861 gagcgggttt ctccccaata gtcaaatgtt ctttcgctat tttgttcaaa ggttctttct
     1921 ttgttaaatc aagaatatca atttagttct tttatttcaa atattttttc gttcgccttg
     1981 gctttgcccc tgcttttttg attgaggctg gtcgggttgt ggggagaccc cacgtattac
     2041 tacgtataca attatcaagt aagtgctatt aggagcgata gcatggcggt ttggcgcgta
     2101 taattgctag tccaagttga tagataaatt tccttctttt ttgtatatcg gcttggtaaa
     2161 gccactgcac gtgccaaaaa aagttccgca aggaccgaac aaagtgagga aggtggagat
     2221 ttttgaatgg ttttttcaaa aatactacct gttccttgcg gaacttttag ctttatcaca
     2281 tatatagaat aagaagggaa catactgtat ttttataaaa tcttcaataa tatcaaggat
     2341 tttgagactt ttcgtagtta ccaattttga taactctagt taccaatttt gataactacg
     2401 aaaaataccg gttaccaatt ttgataacta cgaaaaacat tgatataaca aggtttatag
     2461 ctatttttta tcaaaaacaa ataaacaatt actaattttg ataaaaacaa aaaaagacac
     2521 catttttggt gtctttaatt tatataatta tcgaaaattg ttttaaggaa tgaacaaaat
     2581 acccctactt ctgacgaatc agaaataagg gcaattgttg ttgttaaagt tctaaaagac
     2641 caaggactat tatgagggtg ttgcttcacc ctcataatag tccttgttgg gagtcatgag
     2701 aatttaactt aacattaaat ttttttaggt tttttattat tgctacgtgt aagataaaca
     2761 ataccgttag ttgttttcct tacaatatta agttataaca atgttataac tttgtctact
     2821 gtatacgaca aaaaagatac ctttatagat atctttgtgt tttggcttcc catatttcaa
     2881 tgatccgtcg tgtagaaata tgtttccata atccgtatat ttgaatagat atgaatcgtt
     2941 cttctaattg cacctctagg ggtctatccg acttaatgta ccctctatga atcttaggtg
     3001 catcatcatt aaataccagt cctacctcta ctatattttt atctgcgtac atatatatat
     3061 tcccctttac agtgcctcga aatgaggttg tttacctaac aaaaattata ttacactatg
     3121 atgcacaaac ttaaaagaaa aagctttgat taatgggcaa acatagcgtc tcattatggg
     3181 aaacaaagaa aaaggcgaat cccttactta cataagagat tcgctttttt ctttgtatca
     3241 atctatacat tttttataca ttcctatcat aatgtgagtt ttcgagttct cgtttgttac
     3301 tcaattaacc gaaaaactgt ataaaatctt gcatgctctt agcgttgttt ttttccaaaa
     3361 tactggcggg accccttaaa ataacctctc tggtaaattt tttaaaacag gcattttcat
     3421 agctttggca aacattattt ttaaactatc ttcaacactg tctttactac cagaaatcaa
     3481 aatatgagga tttacaaaaa tcacataaga cttaacatta ttgccttcta aaccgctttc
     3541 cgctttagct agaacacctt tttctacaag gctcttcaca accctactaa cctttgcttt
     3601 acttgttcct aatacggctg caatttcact ttgattgaga cccacaggtt gttttttccg
     3661 aggtacatca acaattgcgt tagatccaat tgctacatga ggaactaaac tcattaaaaa
     3721 gacaatttgt tcacttgtta aatattgatg accttgaata aaactccaat tgtcctgcat
     3781 aatctgtaca aatcgtgcgc gatttttaac tcttctttca ggtaataata ccatcccttg
     3841 cgccttagct ttactttgta actcacaagc tttttccatt tcttcatcag taagaacttg
     3901 cttgcttttt tctatttcta aatccttgtt atgagctttt
//
