LOCUS       NZ_AP022933             4020 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J2 plasmid pJ2_6, complete sequence.
ACCESSION   NZ_AP022933
VERSION     NZ_AP022933.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203331
            Sequence Read Archive: DRR206371, DRR206395
            Assembly: GCF_021655055.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 405x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655055.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:59:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,035
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,646
            CDSs (with protein)               :: 5,646
            Genes (RNA)                       :: 149
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 132 of 240
            Pseudo Genes (incomplete)         :: 117 of 240
            Pseudo Genes (internal stop)      :: 49 of 240
            Pseudo Genes (multiple problems)  :: 47 of 240
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022933.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J2"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ2_6"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            complement(join(3116..4020,1..358))
                     /gene="mobV"
                     /locus_tag="L6497_RS29990"
     CDS             complement(join(3116..4020,1..358))
                     /gene="mobV"
                     /locus_tag="L6497_RS29990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_236794100.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYD
                     LKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFE
                     ESYKLFSERYGKQNIAYTTVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKF
                     PEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVK
                     SDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDND
                     RLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK
                     ISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE
                     FELTHNREVKKERSRDQGMSL"
                     /name="mobV"
     gene            962..1021
                     /gene="ermCL"
                     /locus_tag="L6497_RS29995"
     CDS             962..1021
                     /gene="ermCL"
                     /locus_tag="L6497_RS29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WP_014158491.1"
                     /translation="MGIFSIFVINTVDYQPNKK"
                     /name="ermCL"
     gene            1082..1816
                     /gene="erm(C)"
                     /locus_tag="L6497_RS30000"
                     /old_locus_tag="BCJMU02_p61"
     ARG             1083..1813
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="98.77"
                     /coverage="100.0"
     gene            complement(1889..2872)
                     /locus_tag="L6497_RS30005"
                     /old_locus_tag="BCJMU02_p62"
     CDS             complement(1889..2872)
                     /locus_tag="L6497_RS30005"
                     /old_locus_tag="BCJMU02_p62"
                     /function="involved in plasmid replication"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_014125933.1"
                     /translation="MCNNNSINSDEKSSVLIDKTKSGKIRPWREKKMANVDYFELLHIL
                     EFKKAERVKDCAEVLEYKVSYETGEKTLYRVWFCKSRLCPMCNWRRAMKHGIQSQKVVA
                     EVIKQKPTVRWLFLTLTVRNVYDGTELNKSLSDMSKGFNRMMKYKKINKNLVGFMRATE
                     VTVNNIDNSYNQHMHVLVCVESTYFYNTENYVNQKQWIQFWKRAMKLDYDPNVKVQMIR
                     PKNKHKSDIKSAIDETAKYPVKDTDFMTDDEERNLQRLADLEEGLYRKRLISYGGLLKE
                     IHKELNLDDAEDGDLIHTDDEEKADEDGFSIIAMWNWERKNYFIKE"
                     /name="protein rep"
     ARG             1083..1816
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="94.26"
                     /coverage="100.0"
     Typing          complement(1890..2872)
                     /name="rep_cluster_2104"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..358)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.597"
                     /coverage="99.0"
                     /evalue="9.610000000000001e-64"
     Typing          complement(3120..4018)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.667"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 caatattttg cttgccatat cgttcggaaa ataatttata actttcctca aaaaatcgtt
       61 tttgttctcc tggatccagt tgctcaaaaa aatctcggtc agatgttact agcaactcat
      121 ttacaagaac agcatctttc ctcgtttttc ttgtacctgt tttttgtgat tcaataattt
      181 ctttgacacg ttcgttgtaa tcaatatttt tatcattttt caaatcataa ttttcacgtg
      241 ttcgctcatg gtcaatatca tcattcgttc tactttttcg ctctctttga ttatgaaatt
      301 gcatgccctt tagtccagct gatttcactt tttgcattct acaaactgca taactcatat
      361 gtaaatcgct cctttttagg tggcacaaat gtgaggcatt tttgctcttt tccggcaatt
      421 acttccatgt aaagtataac acactatact ttatatccat aaagtgtgtg ctctgcgagg
      481 ctattggctc tgccaacaaa accctgcata ctgattacca gtatgcacaa aacctttaag
      541 acctttcttt tttttacgag aaaaaagaaa caaaaaaacc tgacctctgc cacctcagca
      601 aaagggggat ttgctctcgt ggctcgttta aaaaagacaa gggacaggtc gtatttttca
      661 aaacgctgcg ctgaaaaatc tccaccttaa acccttgtct tttttatttt gtccgttatg
      721 tttttcgtac cgaaagccgt actcagcaaa atcaaaattt gctcgacttt cggttttcca
      781 ggataacgga caaaaccact taaaataaca aaagacacga gaatgatctc tcgtatcttt
      841 tgtattctta ttcaacaatc gcgcccgatt gcagtataaa tttaacggtc gttcatcatg
      901 ttcttattta tcagagcttg tgctataatt attttaagtt tataagaagg agaattaata
      961 tatgggcatt tttagtattt ttgtaatcaa cacagttgat tatcaaccaa acaaaaaata
     1021 agtggttata atgaatcgtt gataagcaaa attcattata accgcattaa agagggttat
     1081 aatgaacaaa aaaaatataa aagacagtca aaactttatt acttcgaaac gtaatgtaga
     1141 taaaataatg acaaatataa gcttaaatga acatgataat atctttgaaa ttggctcagg
     1201 aaaagggcat tttacccttg aattagtaca aaggtgtaat ttcgtaactg ctattgaaat
     1261 agaccataaa ttatgcaaga ctacagaaaa taaacttgtt aatcacgata attttcaagt
     1321 tttaaacaag gatatattgc agtttaaatt tcctaaaaac caatcctata aaatatttgg
     1381 taatatacct tacaacataa gtacggatat aatacgcaaa gttgtttttg aaagtatagc
     1441 tgatgagagt tatttaatcg tggaatacgg gtttgctaaa aggttattga atacaaagcg
     1501 ctcattggcg ttacttttaa tggcagaagt tgatatttct atattaagta tggttccaag
     1561 agaatatttt catcctaaac ctaaagtgaa tagctcgctt atcagattaa atagaaaaaa
     1621 atcaagaata tcgtacaaag ataaacagaa gtataattat ttcgttatga aatgggttaa
     1681 caaagaatac aagaaaatat ttacaaagaa tcaatttgac atatccttaa aacatgcggg
     1741 aattgacgat ttaaacaata ttagctttga gcaattcttg tctcttttca atagctataa
     1801 attatttaat aagtaagtta aggggtgcgt aaactgcacc ccttaacttg tcttgttttg
     1861 cttaaacaat cttggccctt ttgttgaatt actctttaat aaaataattt ttccgttccc
     1921 aattccacat tgcaataata gaaaagccat cttcatctgc tttttcttca tcatctgtat
     1981 gtattaaatc gccatcttct gcgtcatcaa gatttaattc cttatgaatt tcctttaata
     2041 aaccaccata tgaaattaat ctttttcgat aaagtccttc ctccaaatca gctaaacgct
     2101 gcaaatttct ttcttcgtca tcagtcataa aatctgtatc ttttactgga tattttgcag
     2161 tttcatcaat ggctgatttt atatcagatt tatgtttatt ttttggtcga atcatttgga
     2221 ctttaacgtt tggatcataa tctaatttca tagctctttt ccaaaattga atccattgct
     2281 tttgattcac atagttttca gtgttataaa aataagtgct ttctacacaa accaacacat
     2341 gcatgtgttg gttataagaa ttatctatat tatttacagt tacttctgtc gctcgcataa
     2401 aaccaactaa gtttttatta attttcttat atttcatcat acgattaaaa ccttttgaca
     2461 tatccgataa acttttattt aattcagtac catcataaac atttctaacg gtcaacgtaa
     2521 ggaaaagcca acgaactgtt ggtttttgtt tgataacttc tgcaacaacc ttttgtgact
     2581 gaataccatg cttcattgct ctcctccagt tgcacattgg acataatcga gatttacaaa
     2641 accacacacg atataacgtt ttttctcccg tttcataact cactttatat tcaagtacct
     2701 ctgcacaatc ttttacacgt tcagcctttt taaattcaag aatatgcaag agttcaaaat
     2761 aatctacatt tgccattttc ttttctcgcc aaggtcttat ttttccactt tttgttttgt
     2821 cgattagtac acttgatttt tcatctgaat taatgctatt attattgcac ataattaata
     2881 cagaaacccc ctatgtaaat ttgtttttgt gtggtaactt aaacaataac atagggtatt
     2941 tttctgtgca aggattttta aaggttcatt aaccttataa agccaatcac accaacactt
     3001 taaacgcgat gtaaactata aaaataaaaa cttgttgttt ctatatgtat caagataaga
     3061 aagaacaagt tcaaaaccat caaaaaaaga caccttttca ggtgcttttt ttattttata
     3121 aactcattcc ctgatctcga cttcgttctt tttttacctc tcggttatga gttagttcaa
     3181 attcgttctt tttaggttct aaatcgtgtt tttcttggaa ttgtgctgtt ttatccttta
     3241 ccttgtctac aaacccctta aaaacgtttt taaaggcttt taagccgtct gtacgttcct
     3301 taaggaattc cttagtgctt tcatagatta aactcacatc acgctttaaa tcgcttattt
     3361 tagactttaa agacttgttt tcttcaagca actcattata atcatttaca ttttcattaa
     3421 atcgctctac aagaccacta tatttttctt taacttgccc atgttcttta cttaattttt
     3481 tatattctct cgccatatca gtactcatga gatttctaac atgctgtttt aacctatcgt
     3541 tatctctcgc agcagtcact aagtttttat aatcacgctc cgatataaca acatttttgg
     3601 ttggtttctt ttctgttttc attatttctt ttcccaaacc aaacatggac ttttcacccg
     3661 ttggcacttc aacacttttc atgtgtcgtt tcgctggtac ttctaaatct gatttaactt
     3721 tatcgctata agcagtccat tcatcttttt taactgctaa atttttttct agaaaatcaa
     3781 tctctttttc caaagtttgt tttttaaatt tagctgtctc aatatgttta cggtcagagc
     3841 cacgttcacc acgcttcaac tcaaaaccct gttttttcat atgctcgggg aatttatctt
     3901 gtagccataa cagttcttga cgattaaaca cattttttcc ttgcagtttt ccatcacgca
     3961 taggcacaac acctaaatgc atgtgagggg tttgctcatc attatgaact gttgtataag
//
