LOCUS       NZ_AP022932             5256 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J2 plasmid pJ2_5, complete sequence.
ACCESSION   NZ_AP022932
VERSION     NZ_AP022932.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203331
            Sequence Read Archive: DRR206371, DRR206395
            Assembly: GCF_021655055.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 405x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655055.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:59:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,035
            CDSs (total)                      :: 5,886
            Genes (coding)                    :: 5,646
            CDSs (with protein)               :: 5,646
            Genes (RNA)                       :: 149
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 132 of 240
            Pseudo Genes (incomplete)         :: 117 of 240
            Pseudo Genes (internal stop)      :: 49 of 240
            Pseudo Genes (multiple problems)  :: 47 of 240
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022932.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J2"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ2_5"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            complement(join(4824..5256,1..74))
                     /locus_tag="L6497_RS29965"
     CDS             complement(join(4824..5256,1..74))
                     /locus_tag="L6497_RS29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003352732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048568241.1"
                     /translation="MSKSIEFYRLSNDFYNENTHLVEIMDKEKDGSFKNKERGYGVFLV
                     DIDGLKFALPLRSKMHIKHKDNFTTRIYKDKGKDVRHGLDYSKAIIITEERFVDTSRIF
                     ILENKSDYVKINKDEHHIIKTFEKYVSRYKQGIKKNDSRILAKYRYSTLQNYHAELGLP
                     KLIHN"
                     /name="hypothetical protein"
     gene            820..1281
                     /locus_tag="L6497_RS29970"
                     /old_locus_tag="BCJMU02_p51"
     CDS             820..1281
                     /locus_tag="L6497_RS29970"
                     /old_locus_tag="BCJMU02_p51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360134.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_048568242.1"
                     /translation="MDKNMQYHADTQTLLGKKSKTYVDTETGEKIHVEQITKRAYGQKQ
                     FWKIYLMDFLQVLGVLDSKQVDVFVYILENTEQANNTFIGSQRDIAKECGVSLDTVSRI
                     MKKLQENGFVKQIKRSVYQISPNVMMKGSEHKKSLLLNYYDDSKEQENE"
                     /name="replication/maintenance protein RepL"
     gene            complement(1338..2669)
                     /gene="mobV"
                     /locus_tag="L6497_RS29975"
                     /old_locus_tag="BCJMU02_p52"
     CDS             complement(1338..2669)
                     /gene="mobV"
                     /locus_tag="L6497_RS29975"
                     /old_locus_tag="BCJMU02_p52"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_172795900.1"
                     /translation="MQKMKSTAIKGIQFHNQRERESETNPDIDKEKSHLNYDLINGQKQ
                     IDYNEKIKQVIENNVTSGKKIRKDAVLLSEFLITSDKEFFDKISPKEQERYFETAHEFL
                     ADRYGEKNLIYATVHYDEKTPHMHVGFVPVTEDGRLSAKDFFGQKKQLVSLQDDFNKYL
                     KQNDFDLERGVSSSRKHVETAKYKAQTFQSMEKEAKEKYERTMEHIQEIDDKSKSIKNI
                     EAKKVLGLVGMKEQDYKSLVNYATNGVAYKLEAENLQKELEKTQKEVMQLKADMKIGQD
                     KIRYYYKDIEENLDSLAEKKALQKVKQTDIVKNYGDLIEKYNGLVNKYNDQLKEKKQLK
                     QELDTLKFENRSYQNENRELKLENGKLKEKLMQISKEFSVFKERMGKVLHAQIDRVKTF
                     MRINNVEPKLIKSLDDRQDKLVQDSLAKMEKPQREKQRDMGMER"
                     /name="mobV"
     gene            3590..4141
                     /locus_tag="L6497_RS29980"
                     /old_locus_tag="BCJMU02_p53"
     CDS             3590..4141
                     /locus_tag="L6497_RS29980"
                     /old_locus_tag="BCJMU02_p53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_000028030.1"
                     /translation="MSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVSKGGL
                     LYHFPSKEALIKGMVEDWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLENNNLN
                     SALMATMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFGMAPL
                     NEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            4141..4518
                     /gene="qacH"
                     /locus_tag="L6497_RS29985"
                     /old_locus_tag="BCJMU02_p54"
     ARG             4142..4515
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4142..4518
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="60.75"
                     /coverage="116.82"
ORIGIN
        1 ataatttcta ccaaatgagt attttcattg taaaagtcat tactcaacct gtaaaactct
       61 atacttttag acatcagctt ttctccttac aggtctataa attaaaaaac aggaaaataa
      121 aaaaagacct aataaaaata ttaggtcttt ttttataaac gaacaataag aattttctaa
      181 atcttacatg ggttttaaaa tttttacgtg cggtagtccc tcccgccacg tcatacaagg
      241 gcttgatttt ttaacgtggg gtattccctt tccccattcc atctaacctt agtatatgcc
      301 gatgatccga aaatagtcaa ggtactagct ggtttttaag gttttttcct cttcgtttta
      361 gggtggatgg atggcaaggg ggtgtggggg aatcccccgc atagcgtatc cctataaaga
      421 aaaagagaac aacaacggac ggaagaattg cggttttgtc ggtacaatgg ctagaacaag
      481 ccgattacat aaatcctcgt tttaggtatg gtcggcttgg cgaatatgcc tccaccgaca
      541 aaatttctcc atgcaagggg gaaagggtgg agatttttcg gtggtctttc cgaaaaatac
      601 tacccgtacc ttgcatggag aaaagccctt ttgattaaga agagagtgtt ttttaaaaaa
      661 agtagtctaa aacgttgata cataaaggtt tttggagttt ttgttgtact gatgtaccgt
      721 tacacatgta ccgatatatc aatacaatcg tactgattta atcagaacat atttacagat
      781 ttttatacta catatataat gcgagaagga ggtgaaaaaa tggacaagaa tatgcaatat
      841 catgctgaca cacaaacatt attaggtaaa aaatcaaaaa catacgtcga cacagaaacc
      901 ggtgagaaaa ttcatgtcga gcaaataacc aaaagagcct atggtcaaaa acaattctgg
      961 aagatatatt taatggactt cctgcaagtt ttaggagtgc tagatagtaa acaagttgat
     1021 gtttttgttt acatactaga aaacacagaa caagccaata ataccttcat aggcagtcag
     1081 agagacatcg ctaaagaatg tggggtttca ctagataccg tctcaagaat catgaaaaaa
     1141 ttacaggaga atggatttgt aaaacaaata aaacgaagtg tatatcaaat ttctccaaac
     1201 gtaatgatga aaggttcaga acacaaaaaa agtttactct taaattacta tgacgatagt
     1261 aaggagcaag agaacgaata aaatgcgatt gcaaggcatt ttattcaaaa agcccccgca
     1321 ttagcagggg gcttttttta tcgttccatt cccatatctc gttgtttttc cctttgtggt
     1381 ttctccattt ttgccaatga gtcctggact aatttatctt gtctgtcatc cagggacttt
     1441 atcagttttg gttcaacatt attaatcctc ataaacgtct ttactcggtc aatttgagcg
     1501 tgtaaaactt tccccattct ctctttaaaa acagaaaact ccttgctaat ctgcataagt
     1561 ttttccttca attttccgtt ctctaatttc aattcgcgat tctcattttg gtaagacctg
     1621 ttctcaaact ttagggtgtc caactcttgt tttaactgct ttttctcctt caactggtca
     1681 ttgtatttat taacgagtcc attgtacttt tctatcaagt ctccatagtt ctttacaatg
     1741 tccgtttgct tcactttttg cagtgctttt ttctctgcta atgagtctaa attctcctca
     1801 atatccttgt aataatagcg aattttatct tgtccaatct tcatatctgc tttcagctgc
     1861 ataacctctt tttgagtttt ctccaactcc ttctgcagat tctctgcttc cagtttataa
     1921 gccacaccat ttgtcgcata attcactaga cttttataat cttgctcctt cattccaaca
     1981 agcccaagaa cctttttggc ttcgatattt ttaatcgatt tcgatttgtc atcaatctct
     2041 tgaatatgct ccattgtccg ttcatatttc tctttagctt ctttctccat gctttgaaac
     2101 gtttgagcct tatatttagc cgtttctacg tgtttacggc ttgatgaaac ccctcgctcc
     2161 aagtcaaaat cattttgctt caagtacttg ttaaaatcgt cctgaaggct caccagttgc
     2221 tttttctggc caaagaaatc tttagccgat agtctaccat cctccgttac tggaacaaaa
     2281 cctacgtgca tgtgaggggt tttctcgtca tagtgaacag tcgcataaat taaatttttt
     2341 tcaccatatc gatcagcaag gaactcatga gcagtttcaa agtaccgttc ctgctccttt
     2401 ggtgagatct tgtcaaagaa ctctttatca gaggtgataa ggaattctga tagaagaaca
     2461 gcatctttac gaatcttctt tccagaggtc acattgtttt caatcacttg cttaattttc
     2521 tcgttatagt caatctgctt ttgaccgttt atcaagtcat aattcagatg agatttttcc
     2581 ttatcgatgt cgggattcgt ttcactttcc ctctctcttt ggttgtgaaa ttgaatgcct
     2641 tttatggctg ttgatttcat tttctgcatt cgaataatgg agtaactcat gggggtacgc
     2701 accctttctt aaaatcaata gattaataga aagtgtaaca cactacactt tgcacgcaaa
     2761 gtttcatgtg gtctgcgacg ctaattcggc tttgccgaac catgcaaaag catggtaaaa
     2821 accaaacctc aggcttcgcc cgaacccact ggggaacttt tccccagacc ccctttatcg
     2881 aactccccaa cccctcaatc attgaggggc tccctcgccg gttggcaggt caaaaggtgg
     2941 acttttgaca aaccgagagg gtcttggtgt ggtgagtcta tcaactcctt aatgctccct
     3001 tcggtccgct taccgttgac agacactcca caatgcgaaa ctagcgacga aaagtggtcg
     3061 cctatagttt cactaccaaa agaaccttaa aagccttaga agcgaaaata gagcttcaaa
     3121 aagcaaagaa agagtgtagc taatcttatt ctaagtttag cagcactctt tcgactggtg
     3181 attgactaag ttgttgttaa cttcttgatt ttaaacaaga cgaacagagt agcaacgttg
     3241 ataacaattg cagttgcaga aataacgatg gttagtgtat taaacaatcc tttcccctcc
     3301 attcctgtta ttgcttataa attaaccgat atgacaatgt aggggtagag tcctgaaaat
     3361 gcggatttac aacggtaatg taattttcac ccataaataa agccgatttt aagaaatcga
     3421 ctgttgaact aaagctccct ttactttaag tacatttctt cacaatcttt agattaattt
     3481 attggattat ttttatgtga aagagtgata ttaaataaga gctttaattc gattgacaaa
     3541 aaaaccgtcc agacggtata ttatatgtat ctaagttatg gaggttatta tgtctatttc
     3601 aaagcatgct aataagaaaa aaaatatatt gattgctgca tctgaaattg taaaagaaga
     3661 aggagttgtc aaactcacat tagaggcagt cgcccaaaga gctggcgtga gtaaaggtgg
     3721 attattatac cattttccta gcaaggaagc attaattaaa ggaatggtgg aagattggac
     3781 aaataattat ttcgaatgca ttaatacgtt agttaataac gacgatgata acgcaatagg
     3841 aaaatggaat agggcttatc ttaaatctac attttccgat ttagaaaaca ataatctaaa
     3901 ttccgcacta atggctacta tgtttattaa tccagattta ttagatgaat tccggcagag
     3961 atacgatatt ttgcatacaa agttaataac tgatggaatt gatcctgtca aaataacaat
     4021 tacaagatta tccatagatg gtctttggtt ttcagaaata tttggaatgg caccattaaa
     4081 tgaagaacta aaaacacaag tttttgatga actaataaac atgattcagg aggacgaata
     4141 atgtcatatc tatatttagc acttgcaata gtgggggaga ttattggctc ttccctatta
     4201 aaagcatctg aaggcttttc aaaactctat cctacaatag gggtaataat agctttcgtt
     4261 ggttgttttt tctttttatc cttgtcgcta aaaacaatcc ccttaaacac tgcatatgca
     4321 ttatgggcag gtttaggact tgttttgact acagtaattt cagttttagt ttggaaggaa
     4381 aaacttaaca tggctagcat tgcaggaata acactgattc ttgtaggagt tgtaatattg
     4441 aatttgtttg gaccaggaca tggagaatct ggtcatgaag caagcgaaac tattacaatg
     4501 aatgagacaa tggaataaca cataaaaaag tccttcttaa taaagagaag gactttttag
     4561 cttaattaag ggtatttttt gaagtcctta ttcaacaaac ctgcgtacgt tagtttaagt
     4621 gtatcacttc acaattccaa tagagtagag tttattgaga cataatgaaa ttagcgtttt
     4681 aaatgacttc tgactgtctc ataagacttg tatcaaatgc ttaccgttgt aaatccgcat
     4741 ttccctgacg ctaccccatg tataacaatc aaccaaaaag aaaaagagcg cttttctaat
     4801 cttcatagca ctctttccta attttaatta tgaattaact taggcaatcc tagctccgca
     4861 tgatagtttt gcaaagtaga atatctatac tttgctaaaa tcctagaatc atttttctta
     4921 atcccttgct tatatctcga tacatacttt tcaaaagtct ttattatatg atgttcatcc
     4981 ttgttaattt tcacgtaatc acttttgttt tcaaggatga atattctgga ggtatcaaca
     5041 aatctttctt ctgtaataat aatcgctttt gaataatcaa gaccatgtcg aacatcttta
     5101 cccttatctt tataaattct ggtagtgaaa ttatccttat gctttatatg cattttagat
     5161 ctcaaaggta gggcgaattt caacccatct atatcgacca aaaacacacc ataacctcgc
     5221 tctttatttt taaaactacc atctttttct ttatcc
//
