LOCUS       NZ_AP022926             5049 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J1 plasmid pJ1_5, complete sequence.
ACCESSION   NZ_AP022926
VERSION     NZ_AP022926.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203330
            Sequence Read Archive: DRR206370, DRR206394
            Assembly: GCF_021655035.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 347x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655035.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:47:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,948
            CDSs (total)                      :: 5,796
            Genes (coding)                    :: 5,568
            CDSs (with protein)               :: 5,568
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 100 of 228
            Pseudo Genes (incomplete)         :: 137 of 228
            Pseudo Genes (internal stop)      :: 49 of 228
            Pseudo Genes (multiple problems)  :: 52 of 228
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022926.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J1"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ1_5"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            648..794
                     /locus_tag="L6479_RS29540"
                     /old_locus_tag="BCJMU01_p51"
     CDS             648..794
                     /locus_tag="L6479_RS29540"
                     /old_locus_tag="BCJMU01_p51"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796009.1"
                     /translation="MKKTKKQAKKQPKKQPKKQMKTAMKIVGITKDVVNFVGNLIRIYT
                     FFF"
                     /name="hypothetical protein"
     gene            complement(818..1018)
                     /locus_tag="L6479_RS29545"
                     /old_locus_tag="BCJMU01_p52"
     CDS             complement(818..1018)
                     /locus_tag="L6479_RS29545"
                     /old_locus_tag="BCJMU01_p52"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796011.1"
                     /translation="MDIEAKKEAFVTFLVEKVLFQTFAERTTSLEEKEVYEKFIKLYDQ
                     ELNRLNFELTIASVNRKRANL"
                     /name="hypothetical protein"
     gene            complement(1502..1741)
                     /locus_tag="L6479_RS29550"
                     /old_locus_tag="BCJMU01_p53"
     CDS             complement(1502..1741)
                     /locus_tag="L6479_RS29550"
                     /old_locus_tag="BCJMU01_p53"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796012.1"
                     /translation="MEVKKIKIKPKKIVRNEENHKLEFDKVRLSIEIPISIRDNFKEAV
                     EKNGKTMKEVLEAYMQRYSVKELLTDLKEETIDN"
                     /name="hypothetical protein"
     gene            complement(1722..2060)
                     /locus_tag="L6479_RS29555"
                     /old_locus_tag="BCJMU01_p54"
     CDS             complement(1722..2060)
                     /locus_tag="L6479_RS29555"
                     /old_locus_tag="BCJMU01_p54"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="WP_236796014.1"
                     /translation="MSDNVNFPLRIPRELRDKFKKVSQKTGTSMNSSICQLIAEHIYKM
                     EQLGLKDIEEPNVIQVTPVKEETKAFIKKAIKEADEAKELAETKTKKRLRKSPKKKIGG
                     ETDGSKKD"
                     /name="Arc family DNA-binding protein"
     gene            complement(2072..2395)
                     /locus_tag="L6479_RS29560"
                     /old_locus_tag="BCJMU01_p55"
     CDS             complement(2072..2395)
                     /locus_tag="L6479_RS29560"
                     /old_locus_tag="BCJMU01_p55"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796016.1"
                     /translation="MMMNLLSLQQEANSVIQDLNGKDISSMSLIQKANYSNRIVKVRMS
                     LEAYRNTPGVNEFINALDHLRFKLAEVQQQETRQQANKRRAEIIQNGIKETPYPTMPMF
                     LNN"
                     /name="hypothetical protein"
     gene            complement(2813..3658)
                     /locus_tag="L6479_RS29565"
                     /old_locus_tag="BCJMU01_p56"
     CDS             complement(2813..3658)
                     /locus_tag="L6479_RS29565"
                     /old_locus_tag="BCJMU01_p56"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_236796018.1"
                     /translation="METDISIDRLTLVGYDTGMIEGYLKYSEYISFVGFSKNYGFRHSY
                     QGIKGELLEIGEKGKVRLDFNPNTANMEQVRDVVSRLKYIYLTRLDIAIDYFGVDLSEV
                     EWTSPKRRKQNLWLDPEGQQETRYIGAPSSERRYRIYNKKLERLEKKEELDPRAVNGQW
                     RVEVQQRFKESNNILDPIDYFTGDLFDIRPYRKVVDLSHIEKPMERIIIAGLMARPSEL
                     KNLSKNARGKYKKMIQEAREKSGVAINPEPIEVYTKEKDLLAEQLTSLFAHCAKSVAQV
                     "
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(3861..4583)
                     /locus_tag="L6479_RS29570"
                     /old_locus_tag="BCJMU01_p57"
     CDS             complement(3861..4583)
                     /locus_tag="L6479_RS29570"
                     /old_locus_tag="BCJMU01_p57"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236796021.1"
                     /translation="MKKAFAENYINPVVERFSELDTPNGGFYTSIPQAVQYCWFLDDKE
                     LSVLLALSTYADENGRCKVYQRTLARKVKIKSTTTLRTKLKSLVKKRFILQVNTDGESS
                     IYQIKDYSTNPYILLSEAIEKVEQVAKKKYGVSVSKIAETMDELIASKIDYMAMITKIE
                     ESFNGYDVALKSVQGYVNEALGLEMDISLQTKKPRRERGFKMSKVIKPKQPKRNREFII
                     SEGESEAPVKREGLRLRL"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4676..4828)
                     /locus_tag="L6479_RS29575"
                     /old_locus_tag="BCJMU01_p58"
     CDS             complement(4676..4828)
                     /locus_tag="L6479_RS29575"
                     /old_locus_tag="BCJMU01_p58"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796023.1"
                     /translation="MPKVVSSKEKMQPKTFKLHPRTIEQIKELAEKKQMTQAEVIDKAL
                     KLLAR"
                     /name="hypothetical protein"
ORIGIN
        1 gggtttttcg acgtgtcgaa catccctata gttataaaaa atcagagcaa gaactagaat
       61 acatagttct taatcatccg aacaaaaccc aaactttttg ggagactatt ttcaaatgaa
      121 aaatcgttat tgttttgtat agaaagagta atggcacttt tgagctacta tttgggctac
      181 taaattagat acctttttca atctttatcc aagatacact tttctataga ggataatctc
      241 gataaaaaga cggataaatc acggataaat taaggataaa gagaaggata aatactatga
      301 agaaaagctc taaaatttaa ctaagaaaag aatcacacca acacgaacaa tataatgtgc
      361 ctatctcttt gaagaaaaaa actcttaata caaatctctc aaaaagtgtc acaaaacata
      421 gatgaaaaga cgaatagaaa actaggaggt gttattccat tgacaatcca aattgatatt
      481 tttactcaag ttttatacat atagcaaacc aaaaaacctt agtatctgcg cctatgttta
      541 taaaagaaat ttttttgaaa aagaataaaa aaaataagga tttctcaaaa aacgtcacaa
      601 gtaatagata gcaagacgaa caactactaa atagggggaa ttaatttatg aagaaaacta
      661 aaaaacaagc aaaaaaacaa cctaagaaac aacctaagaa acaaatgaag acagcaatga
      721 aaatagtcgg aataacaaaa gacgtggtca attttgttgg aaatctaatc cgaatttata
      781 cattcttctt ctaaacaaaa agagctaatc tataacatta taggttggct ctttttctat
      841 ttacagatgc tattgtcaat tcgaaattta gtctatttaa ttcttgatca tatagcttaa
      901 taaacttttc atacacctct ttttcttcta aagaagtagt cctttctgca aatgtttgaa
      961 atagaacttt ctctacaagg aaggtcacaa atgcttcttt cttagcttct atatccataa
     1021 cttacttcct ccaataagcg gaattcaatc attgacaacg agcctccagt ccgtgggtca
     1081 taagccaatg ccccactcct tcaactcgct aacgctcaaa caaggattgg caagccataa
     1141 caggctacca cggacattct cattgtaaat gaaccgctac gctttgattc cttagtggtc
     1201 ttccctaatc ttcgctaata ctcgatgaaa accaattaat ttaaccctta attcttcgcc
     1261 aatcctcgtt aaacctcgcc atttcttccc taaaaaccga cataccgtaa accgcaaaga
     1321 ctatgccctc acatgaatgc ctcctggttc accactatct taggaagaat acttatagtt
     1381 gtttagcgta aatttagcga tatttttatt gataggtcgt gttttttcta cttttaccaa
     1441 ctataaaatt taagccttaa aaatgtacat gtattcaagg tttaaacgag gtattcagat
     1501 tctaattatc tattgtttct tcttttaaat ccgttaatag ctctttaaca ctataccttt
     1561 gcatatatgc ttctaatact tccttcatcg tcttcccgtt cttttctacc gcttctttaa
     1621 agttatctct aatagaaata gggatttcaa tgcttaatct taccttatca aattctaatt
     1681 tatgattttc ttcattacgc actatttttt taggtttgat cttaatcttt tttacttcca
     1741 tctgtttcac cgcctatttt cttcttgggc gactttctta atcttttttt cgtcttagtt
     1801 tcagctaatt ccttagcttc atcagcttct tttatagcct tctttataaa cgcctttgtt
     1861 tcttctttaa ccggcgtaac ttgaattaca ttaggttctt ctatatcctt caatcctaat
     1921 tgctccattt tataaatgtg ttccgctatt agttggcaaa ttgatgagtt catactcgtc
     1981 ccagtcttct gtgaaacctt tttaaactta tcccgcaatt cacgaggtat tctcaatgga
     2041 aaattaacat tatcgctcat atcctatcct cctaattatt tagaaacatt ggcatcgttg
     2101 ggtacggtgt ttcttttatt ccattttgaa ttatttcagc tcttctttta ttagcctgtt
     2161 gtcgtgtttc ctgttgttgt acttcagcta acttaaatct taagtggtcc aatgcattta
     2221 taaattcatt tactcccgga gtatttctat atgcctcaag gctcattcta actttcacta
     2281 ttcgattaga ataattagcc ttctggatca aactcattga agaaatatcc ttaccgttta
     2341 aatcttgaat cacactgtta gcttcctgtt gtaaacttaa caaattcatc atcataatat
     2401 tttccctctt ttctgttgtg ataatcaata cgactattaa attgattatc aatatgatag
     2461 tcatttataa aaacatcgta actcttaacc aaaaatctgt atactgggtt acttaaaaaa
     2521 tttaaaattt atttttaaat tgtcacctta cctatataaa aattaacaaa atcgagaggg
     2581 tgtaggagcg cattttcaga tacagaacct aataagcggt gggtggggtg tttttcaaac
     2641 aatagcctaa atagacaact attaatccta aagcacgagc taacgcaata aacttaaagg
     2701 aaatacatat attcttaacg aatcccctta aaacaaagaa aaagagaggt gaattccctc
     2761 tcttcttttg ctatactaac attagatatt tacttgttat aagttttaaa gtttacactt
     2821 gtgctacgct cttagcacag tgggcgaaca ggcttgttag ttgctcagct aacaagtctt
     2881 tttcttttgt ataaacttct attggttcag gatttatcgc tacaccgctt ttttccctag
     2941 cctcttgtat catcttctta tacttacctc tagcgttctt actaagattc ttaagttcac
     3001 taggtcttgc cattaatccc gctattataa ttcgttccat aggcttttct atatgagata
     3061 aatcaaccac ctttcgatat ggtctaatat cgaatagatc acctgtaaaa taatcaatag
     3121 ggtctaatat gttattggac tctttaaaac gttgttgtac ctcaacacgc cactgtccat
     3181 taactgctct agggtctaac tcttctttct tttctaaccg ttctaacttc ttgttataaa
     3241 tacgatacct tctctcactt gatggcgcac ctatgtatct cgtttcttgt tgcccttctg
     3301 ggtctaacca taagttttgt tttcttcgtt tagggcttgt ccactcaacc tcagataaat
     3361 ctacaccaaa ataatctatc gctatatcta atcgcgttaa atagatgtat ttcagtcgag
     3421 aaacgacgtc tcttacctgc tccatatttg cggtattagg gttaaaatct aaacgtacct
     3481 tccctttctc gcctatctca agcaattctc cttttatccc ttggtatgaa tgacgaaaac
     3541 cataattctt agaaaacccc acgaatgaaa tatattccga gtatttaaga tacccttcta
     3601 tcattccagt atcgtatcca acgagagtta ggcggtctat actaatatct gtttccatcc
     3661 taaacactca tttccgtttt atttgctatc ttagtatgct tcagtaattt ctttgataga
     3721 tatatatatt aatttctttt tagtatttta tatttagtag taatcaaatt ttgattaaga
     3781 ggtacacaaa aattgtgtaa ggggtaatca atttttgact aggtattcaa gtcctataag
     3841 gtaatatata attaccttat tcaaagtcgt aatctaagac cttcccgttt cacaggagct
     3901 tcagattcac cctctgaaat aataaattct ctgttacgtt taggttgctt aggtttaata
     3961 actttactca tcttaaaacc tcgttcccgt cgaggttttt tcgtttgtaa agaaatatcc
     4021 atttctaatc ctaaagcctc atttacataa ccttgtacag attttaaagc tacatcataa
     4081 ccattaaaac tttcctctat cttagttatc atagccatat aatctatctt actagcaatt
     4141 aattcatcca tcgtttcagc tattttagat actgaaacac catatttctt tttagctacc
     4201 tgttcgactt tttcaatagc ctcactaaga agaatataag gattggtaga ataatcttta
     4261 atctgataaa tactagattc tccatctgta ttaacctgca atataaaacg cttcttcact
     4321 aaacttttca atttagttcg taatgttgtt gtacttttaa tcttaacttt cctagctaac
     4381 gttcgttgat ataccttgca acgtccattt tcgtctgcat aggtcgatag tgctaataat
     4441 acacttaact ctttatcatc taaaaaccaa cagtattgaa ctgcttgtgg aatggaagta
     4501 tagaaaccgc catttggagt atctaattca ctaaaacgtt ccacaacagg attaatataa
     4561 ttctctgcaa atgccttttt cattacagat caccttttcc ttttaatttt ccctccttgt
     4621 tatcttgttt gctatgttag tatgctacgt tatttgcgat gggatatata tttttttatc
     4681 gtgctaataa ctttaacgcc ttatcaatca cttcagcttg cgtcatttgc ttcttttcag
     4741 ctaattcctt tatttgttca atcgtcctag gatgcaattt aaatgttttg ggttgcatct
     4801 tttccttaga actaactact ttcggcataa tatcactcct caacacaatg ataggtactc
     4861 cctaaaaaca gcttaacatc aacgattcta tttgtccact tcaaaaacag tcaaaaaaca
     4921 cataaaaaaa tagaacaaac taataaggac atttttttga tcaaaaaaga aaggagaaaa
     4981 cctaaaacaa aaaacatttt gatatggtga atccctcgaa agaggacgct aacccgctgt
     5041 tagagaagc
//
