LOCUS       NZ_AP022925             8220 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain J1 plasmid pJ1_4, complete sequence.
ACCESSION   NZ_AP022925
VERSION     NZ_AP022925.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203330
            Sequence Read Archive: DRR206370, DRR206394
            Assembly: GCF_021655035.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 347x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655035.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:47:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,948
            CDSs (total)                      :: 5,796
            Genes (coding)                    :: 5,568
            CDSs (with protein)               :: 5,568
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 100 of 228
            Pseudo Genes (incomplete)         :: 137 of 228
            Pseudo Genes (internal stop)      :: 49 of 228
            Pseudo Genes (multiple problems)  :: 52 of 228
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022925.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="J1"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pJ1_4"
                     /geo_loc_name="Japan:Tochigi"
                     /collection_date="2006"
     gene            complement(join(8122..8220,1..249))
                     /locus_tag="L6479_RS29490"
     CDS             complement(join(8122..8220,1..249))
                     /locus_tag="L6479_RS29490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236798225.1"
                     /translation="MANYQKERLECHVYGRLIAIFICSSTMFKICKLLLQKNKRELSEY
                     KAIGMIQDHVSLLYQAIQRNTQNLTKILIRLFDLLQKNGRKSHRYEKKTIFDIMGVVYE
                     YNGFGKQKKAA"
                     /name="hypothetical protein"
     gene            complement(485..>757)
                     /locus_tag="L6479_RS29495"
                     /old_locus_tag="BCJMU01_p41"
                     /pseudo=""
     CDS             complement(485..>757)
                     /locus_tag="L6479_RS29495"
                     /old_locus_tag="BCJMU01_p41"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(775..1053)
                     /locus_tag="L6479_RS29500"
                     /old_locus_tag="BCJMU01_p42"
     CDS             complement(775..1053)
                     /locus_tag="L6479_RS29500"
                     /old_locus_tag="BCJMU01_p42"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_236798226.1"
                     /translation="MKYIFSALWKSKICETSAISSAALTYFQRIRILDATIFQVPKHLA
                     HVYPGSGGCAQTAGIKIQLEYDLHSGQFLNFQIGPGKIMIRPLEQNA"
                     /name="transposase"
     gene            complement(1019..1330)
                     /locus_tag="L6479_RS29505"
                     /old_locus_tag="BCJMU01_p43"
     CDS             complement(1019..1330)
                     /locus_tag="L6479_RS29505"
                     /old_locus_tag="BCJMU01_p43"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236798227.1"
                     /translation="MNLSIQDELQLFSEELYRHLTPSLLEELAKELGFVKRKRKFSGNE
                     LATICIWVSQRTASDSLVRLCSQLHAATGTLMSPEGLNKRFDKKRLNFEIYFFGIMEK"
                     /name="hypothetical protein"
     gene            complement(1660..2037)
                     /gene="qacH"
                     /locus_tag="L6479_RS29510"
                     /old_locus_tag="BCJMU01_p44"
     ARG             complement(1664..2037)
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2037..2588)
                     /locus_tag="L6479_RS29515"
                     /old_locus_tag="BCJMU01_p45"
     CDS             complement(2037..2588)
                     /locus_tag="L6479_RS29515"
                     /old_locus_tag="BCJMU01_p45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_000028030.1"
                     /translation="MSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVSKGGL
                     LYHFPSKEALIKGMVEDWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLENNNLN
                     SALMATMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFGMAPL
                     NEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            3783..4895
                     /locus_tag="L6479_RS29520"
                     /old_locus_tag="BCJMU01_p46"
     CDS             3783..4895
                     /locus_tag="L6479_RS29520"
                     /old_locus_tag="BCJMU01_p46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002019875.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_000036645.1"
                     /translation="MSKITNDIDLKIDEVYEKVKDMKITFDENNTTKKQLTDSTINTYC
                     GIVKSYNNWLIQTHGISVDRAKPRHAMEYLETKIQAFEKGNASAFSMRQMAHAFHSFRV
                     ASAETGTFKHTCKVADKRDMLNTLTERGIFRKSADSTVLKANHSDYEKVQKELNVATTS
                     KHLREDIKTVHELQRYAGMRVHEAVKLKVSDIDFKSGTVYIKGKGGHERTVKIESKELL
                     EKLKNLCTGKSERSQVIIARKRDKNQLTNEEFGKRAKEEIRGAAVNAGVDRNGKRYTTH
                     SGRKAYAQAQMDKYKSYSKNQLKKEVAKRCQDKKTKQKYDAVIKNIRKKITEGSKNEGR
                     KLTNKEMCQFLVSLDLGHYRLDVVRYYADY"
                     /name="tyrosine-type recombinase/integrase"
     gene            5708..6115
                     /locus_tag="L6479_RS29525"
                     /old_locus_tag="BCJMU01_p47"
     CDS             5708..6115
                     /locus_tag="L6479_RS29525"
                     /old_locus_tag="BCJMU01_p47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000037919.1"
                     /translation="MSKKISIEEMTQEMKEKFTISLEDAHKILLDHGVTKSTNTQVTMR
                     WIRDGKIDALLIGKGRVETRKWLINKESLMEFVAFERLTLGDFKAMKEELEMLRAFKFE
                     VEKKPKRATGTRQKKDAATNTSKEKKEVPTK"
                     /name="hypothetical protein"
     gene            6129..6566
                     /locus_tag="L6479_RS29530"
                     /old_locus_tag="BCJMU01_p48"
     CDS             6129..6566
                     /locus_tag="L6479_RS29530"
                     /old_locus_tag="BCJMU01_p48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000094076.1"
                     /translation="MSSFRWYEENEVIGKTIIEEDDLTLLEAGKIYIDAIFCERIRFPV
                     QKNEQPIGFNFVYEYNVKKYFGLYDRSFMKIIKQEDLEEYHMRMIDFHVHRLSLGEELD
                     DCPIIGICYGSGPLITPRLEQVYDVSKTKAYRKYRLEKLFD"
                     /name="hypothetical protein"
     gene            6817..7437
                     /locus_tag="L6479_RS29535"
                     /old_locus_tag="BCJMU01_p49"
     CDS             6817..7437
                     /locus_tag="L6479_RS29535"
                     /old_locus_tag="BCJMU01_p49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000963189.1"
                     /translation="MAIDFSQADRNARKRDLEKEKFFSDEEWELANDLQSRANARGMKL
                     VPERKIKNRAKFAQMLQDNVDYLEEIDYLDDKEIIFLWKISRKVGFLSNCLVDDIHKKS
                     PVPLTQTDIATLLKRTKNNVNPIVKSLVAKGILAVSKTGVQGNNSRANVYFMNPNILYA
                     GSKDDVNLTLQAMFSKVPKALKDMPVKLFELSCHSEEEEEKTN"
                     /name="hypothetical protein"
     ARG             complement(1661..2037)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="60.75"
                     /coverage="116.82"
ORIGIN
        1 attcttctgt aggaggtcga acaaacggat taaaatcttt gttagatttt gggtgtttct
       61 ctgtatcgct tgatataaca gcgatacatg atcttgaatc atcccaatgg ctttatattc
      121 acttaattct cttttgttct tttgcaaaag aagtttacat atcttaaaca tcgtagaaga
      181 acagataaag atggcaataa gtcttccata cacatgacat tctaatcgct ctttttgata
      241 gtttgccaat gatgaatttg aaataaggat ttccacgttt taaaaataat ttctatttgc
      301 cagcgaaggg agtaaagtca tgtatttgtt ccatcggaac aactccttca ggcgtatttg
      361 agacatatat gttgataccc gttaatcgtt tactcttttc tgaaaatgta ataccctttt
      421 tactttcagt gtaactttgt ttttctacgt tcctgtatct gtttgtctgt taaacgatag
      481 ataattacac gtgtgaatag ttttgattct ttccaatgta agcttctttt atttcgtatg
      541 tttgaccggg ttttaaattg ttcataatat gttctaaatc aacttggata tactgtgatt
      601 gcttttttac ggttccgttt cgaaagtatt ctggaaacgg attttttaca tacacggtat
      661 ggtttaattt taatctggat atataataag cacctcgttg atccatttga tctaagtctt
      721 ctaatgaaaa ataacctaaa tcacggatac ataaatcgct tggacgtaat gtgtctaagc
      781 attctgttcc aaaggtctta tcattatttt tcctggacct atctgaaagt ttagaaactg
      841 tccgctatgt aagtcatatt ctaattgaat cttgataccc gctgtttgtg cacaaccacc
      901 tgatccagga tatacatggg ctaaatgttt tggtacttgg aaaatcgtcg catctaaaat
      961 acgaattcgt tgaaaatacg tgagcgccgc gcttgaaatg gccgatgttt cacagatttt
     1021 acttttccat aatgccgaaa aaatatattt caaaattcaa ccgcttttta tcaaagcgtt
     1081 tattgagtcc ttctggactc ataagagtgc ctgtggccgc atgtaattga ctgcatagtc
     1141 gaacaagcga gtcactcgct gtacgttgac ttacccaaat acatatggta gctaattcat
     1201 ttccagaaaa ctttcgtttt ctttttacaa aacctaattc ttttgcgagt tcttccaaaa
     1261 gagaaggggt taaatgacga tacagttctt cagaaaatag ttgtaattca tcttgaatcg
     1321 aaagattcat aaaaaatgtc accctttcta gctagctttt tagaaagaat aacgtttttt
     1381 gtcacttggg ggtagtgaaa attcttaagt tgatgggcat ggggtagcgt cagggaaatg
     1441 cggatttaca acggtaagca tttgatacaa gtcttatgag acagtcagaa gtcatttaaa
     1501 acgctaattt cattatgtct caataaactc tactctattg gaattgtgaa gtgatacact
     1561 taaactaacg tacgcaggtt tgttgaataa ggacttcaaa aaataccctt aattaagcta
     1621 aaaagtcctt ctctttatta agaaggactt ttttatgtgt tattccattg tctcattcat
     1681 tgtaatagtt tcgcttgctt catgaccaga ttctccatgt cctggtccaa acaaattcaa
     1741 tattacaact cctacaagaa tcagtgttat tcctgcaatg ctagccatgt taagtttttc
     1801 cttccaaact aaaactgaaa ttactgtagt caaaacaagt cctaaacctg cccataatgc
     1861 atatgcagtg tttaagggga ttgtttttag cgacaaggat aaaaagaaaa aacaaccaac
     1921 gaaagctatt attaccccta ttgtaggata gagttttgaa aagccttcag atgcttttaa
     1981 tagggaagag ccaataatct cccccactat tgcaagtgct aaatatagat atgacattat
     2041 tcgtcctcct gaatcatgtt tattagttca tcaaaaactt gtgtttttag ttcttcattt
     2101 aatggtgcca ttccaaatat ttctgaaaac caaagaccat ctatggataa tcttgtaatt
     2161 gttattttga caggatcaat tccatcagtt attaactttg tatgcaaaat atcgtatctc
     2221 tgccggaatt catctaataa atctggatta ataaacatag tagccattag tgcggaattt
     2281 agattattgt tttctaaatc ggaaaatgta gatttaagat aagccctatt ccattttcct
     2341 attgcgttat catcgtcgtt attaactaac gtattaatgc attcgaaata attatttgtc
     2401 caatcttcca ccattccttt aattaatgct tccttgctag gaaaatggta taataatcca
     2461 cctttactca cgccagctct ttgggcgact gcctctaatg tgagtttgac aactccttct
     2521 tcttttacaa tttcagatgc agcaatcaat atattttttt tcttattagc atgctttgaa
     2581 atagacataa taacctccat aacttagata catataatat accgtctgga cggttttttt
     2641 gtcaatcgaa ttaaagctct tatttaatat cactctttca cataaaaata atccaataaa
     2701 ttaatctaaa gattgtgaag aaatgtactt aaagtaaagg gagctttagt tcaacagtcg
     2761 atttcttaaa atcggcttta tttatgggtg aaaattacat taccgttgta aatccgcatt
     2821 ttcaggactc taccccggaa agaaaaacat ttaaggtcgt aagacctttt caaaagcata
     2881 aaaaaataag gttttttcat caaaatcagt tgattaatgg taaaatttgt atatattcat
     2941 agtaatttta aaaccaatta cttatttgtt taaacgcttg cggttaagca aatatgaaat
     3001 acgaaatttt atactatgaa tcttgggacg tgattcaagt aatcgtgtat tttacaaaac
     3061 taaaaaattc acgtcataaa aatgaaaggg gatcattaca atagcaaagc aaaatgtttt
     3121 taagacgtgg gaaaaaataa tctaagaaag gagcaatata aagctttccc aagtagtttt
     3181 ataaatagct ttaaaagaaa atagaattct tgcttgcaag aatggaaaaa aatattataa
     3241 aaaataaaaa tttaaggaga tgacaaatca aaaaatacga agggaacaag ttttacaatt
     3301 aaatattttt acaactgaat aaatgtaaat ctgtaacaaa ggtttttaac cacaaaacaa
     3361 atcgcaagaa cagtaatttc tgggttactc aaaaattcca gaccgcaata ccgttgtacg
     3421 ctcatttgac tatacatttt ggtgcaaaat gtatgggaga gccttggagt ctatttatag
     3481 acgccaatcg caacaagatg gcgtagctat cttgagcctt tgtcttttaa tcttttgacc
     3541 ttgatttttg cttttggtct tttgattttg actttgactc tagtgagctt aaatcgcccg
     3601 aagggagatt tcgcgattgc ttttgtgtgt ttaatttggg tggttcggtc tatttttttt
     3661 ggtctttaaa aaaaatagat cgaacccacc caaatgagac ctcgtttttg ttgtgccttg
     3721 atattgtaag gttcacgggg gttgggttct tgctcttgat tttgtatata gggggtgacg
     3781 aaatgagcaa aattacaaat gatattgatt taaaaattga tgaagtctat gaaaaagtta
     3841 aagatatgaa aataactttt gatgaaaata atacaaccaa aaaacaatta actgattcaa
     3901 caataaacac atattgcgga atcgtgaaat catataacaa ttggttaata caaacgcatg
     3961 gaattagcgt ggatagggcg aaacctcgcc acgcaatgga atatctcgaa acaaaaattc
     4021 aagcctttga aaaaggaaat gcttccgcat tttctatgcg ccaaatggcg catgcgtttc
     4081 attcttttcg tgttgcgagt gctgaaacag gaacgtttaa acatacatgt aaggtcgctg
     4141 ataaacgaga tatgttaaat acactcacag agcgtggaat cttccgtaaa tcggctgatt
     4201 cgacggtatt aaaggcaaat cattcagact atgaaaaagt tcaaaaagaa ctaaatgtag
     4261 caacgacaag taaacatttg agagaagata taaaaacagt acacgaatta cagcggtacg
     4321 ctggaatgcg tgttcacgaa gccgtaaaac taaaagtgag tgatatagac tttaagagtg
     4381 gcactgtcta tataaaaggc aaaggcgggc acgagagaac ggttaaaatt gaaagtaaag
     4441 aattactaga gaagttgaaa aatttatgca ctggaaaaag tgaaagaagc caggtaatta
     4501 ttgctcgaaa acgtgataaa aatcaattaa caaatgagga atttggaaaa cgtgcaaaag
     4561 aagaaatacg aggtgcagcc gtcaatgctg gggtggatcg taacggaaaa agatacacaa
     4621 cacattcagg tcgcaaagca tatgcacaag cgcaaatgga taaatacaaa agttacagta
     4681 agaatcaatt aaaaaaagaa gttgcaaaac gttgtcagga caagaaaaca aagcaaaaat
     4741 atgatgcagt tataaaaaat atccgtaaaa aaataaccga aggttcgaag aacgaagggc
     4801 gtaaacttac aaataaagag atgtgccagt ttctagttag cttggatttg ggtcactatc
     4861 gtttagatgt tgtacgatat tatgctgatt attgataggt tttgacggaa aatcgggaag
     4921 acgagtgcgg agcacagttg gtactggcgc actggcttag aaacacaagg gctagagcct
     4981 cgttgtcaag ccatctttcc cggctttaca atgagaaagc cttgtgttag cctctcatgg
     5041 caatgccagt accttgtgcg gattcgagga aatcgccaaa aaacgggtcg atgaactaag
     5101 aagatacacg agagttttcc aaatcctcgc catttctcgc tttttttcga aaatcaaaaa
     5161 aaaatgaaac gcctattaaa tttactatac agtaaaagta ttttaaaata catttattgt
     5221 atagtaaatt tatttttgag taaaagtatt ttaaagtaat tttaatagtt ttacacaata
     5281 gtaaatttat atacctaaat tagatttaca caaaagtaaa aatatagata aaaatacatg
     5341 tattgtctcc gcaatttatt tttagtaatc gcaaaaaatc gttgatttaa caacgtttaa
     5401 gagatataaa atacatgtac aaaatagtaa agtcatggca ataaatagat ttacacaaaa
     5461 gtaaaagtaa tgacctaaaa tggatttaca taaaaataaa tttaatgtat aataaattta
     5521 aagaaggata tatataattt tgagtaaata taattaatag taaatgtaaa atagattaaa
     5581 tatatttttt aataaattta tttaaaatta tatttacttt tcaatacatt tactgtatat
     5641 taaaaatata gatggataca tttaattaaa aatacatgta cttaattgta attaggagga
     5701 aaaaacaatg tcaaaaaaaa tctcaattga agaaatgacg caagaaatga aggaaaaatt
     5761 cacgatttca ctggaagatg cacataaaat ccttttggat catggcgtaa cgaaaagtac
     5821 gaatacacaa gttacaatgc gttggattcg tgatgggaaa attgatgcat tattgattgg
     5881 aaaaggtcgt gtggagactc gaaaatggct tattaacaag gaaagtctaa tggaattcgt
     5941 tgcattcgag cgtttaacgt taggtgattt caaagcaatg aaagaagaat tagaaatgtt
     6001 acgtgctttt aaattcgaag tagagaaaaa gccaaagcgt gctactggta cacgtcaaaa
     6061 gaaagatgca gcaacaaata cttcaaaaga aaagaaagaa gtgccaacaa aataaagagg
     6121 tgaattgcat gtcatctttt agatggtatg aagaaaatga agtgattgga aaaactataa
     6181 tagaagaaga tgatttgact ttgctagaag ctgggaaaat atatattgat gcaatatttt
     6241 gtgagagaat aaggtttcca gttcaaaaaa acgaacagcc aattggattt aattttgttt
     6301 atgaatataa tgtaaaaaaa tatttcggat tatatgacag aagtttcatg aagataatta
     6361 aacaagaaga tttagaagaa tatcacatga gaatgataga ttttcatgtg cacagattat
     6421 ctttagggga agaattagat gattgtccaa tcattggtat ttgttatgga agtggacctt
     6481 tgataacacc gaggttggaa caagtttatg atgtttcgaa aacaaaagca tacagaaagt
     6541 atcgattaga aaaattattc gattaggtaa agcatcttac aaaatttgag tttttaagct
     6601 agaattgaat agacaagcat aaaaaaaagc cgagtgttgg agcactcgac tttgggtgat
     6661 tcatcgtaat ttatcaccaa aacgttgatt tctagtttta attttaatga attttcattt
     6721 aaaaatcaag ggtaaaaccc tttacggata ataaaaaaat aacggtgaat ctcctagaat
     6781 gcttgttgca ttttcttcag gagaggagca aaatcaatgg caattgattt tagtcaagcg
     6841 gatcgaaacg ctagaaaaag agacttagaa aaagaaaagt ttttttcaga tgaagaatgg
     6901 gaattagcaa atgatttgca atcaagagct aacgcgcgag gaatgaaact tgttccagag
     6961 cgtaaaatca agaatcgagc aaaatttgct caaatgcttc aggataacgt tgattatctc
     7021 gaagagattg attatttaga tgataaagag attatatttt tatggaaaat cagtcgtaaa
     7081 gttggttttt tatcaaattg tcttgtggat gatattcata aaaaaagtcc agttccttta
     7141 acacaaacag atatcgcaac tttgttgaaa agaactaaaa ataatgtcaa tcctattgtt
     7201 aaatctttag tagcaaaagg catactggct gtttcaaaaa caggtgttca aggtaataat
     7261 tcgagagcaa atgtgtattt tatgaatccg aacattttgt acgccggtag caaagatgat
     7321 gtcaatttaa cattacaagc gatgttttca aaagttccta aagcattaaa ggatatgcct
     7381 gtaaaattat tcgaactatc atgtcattca gaagaagaag aagagaaaac aaattaataa
     7441 taattaatac atgtattttt taatacatgt atttttttat ctttcaatta tgtaaaataa
     7501 tgtaaaaaat gtaaaaaatc cggtgttctt aaattcaaga actatggttc ttaaattcaa
     7561 gaactacgaa aagtgcaaca aaccattgat attattgact ttttaataca ttaacaatgt
     7621 tgttctatct cactctctat taacgctaat tttgagtaaa gaaacgagta gtattttttg
     7681 caaaaaccgc aaaaaatctc cactctttat cttgactcaa aattctccgc aattggcatg
     7741 gcttgtgcca agcgataaac caaaaaagtt atcacttttt cggattatca cttgaacaat
     7801 ccattttaat tgctccgtcg cttagtgcgt acatttccgt tctatcaata ttttgtgtaa
     7861 taaaaaccct tggggctagc cccaaacccc agccagctca acccaaaaaa gcatatgaaa
     7921 accgtatcaa atttaatacg gtaaaagaat aaaagactta aaaacatttt tgcttaggaa
     7981 aggggtagcg tcacatgccc atcaacttaa gaacgaaaac aaaatgaact ttcgttcaaa
     8041 caaactaaaa aacacgtaat gtacttttgg tattgaaaag tacacacacc aaataaactc
     8101 taacgagttt cattttaaaa attatgcagc tttcttttgt tttccaaacc cattatactc
     8161 atagacaaca cccataatat caaagatagt tttcttctca tatcgatgag atttccgtcc
//
