LOCUS       NZ_AP022920             5049 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0105 plasmid pMRY14-0105_5, complete
            sequence.
ACCESSION   NZ_AP022920
VERSION     NZ_AP022920.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203329
            Sequence Read Archive: DRR206369, DRR206393
            Assembly: GCF_021655015.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 354x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655015.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:26:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,896
            CDSs (total)                      :: 5,744
            Genes (coding)                    :: 5,528
            CDSs (with protein)               :: 5,528
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 95 of 216
            Pseudo Genes (incomplete)         :: 131 of 216
            Pseudo Genes (internal stop)      :: 48 of 216
            Pseudo Genes (multiple problems)  :: 51 of 216
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022920.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0105"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0105_5"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            complement(join(4946..5049,1..43))
                     /locus_tag="L6451_RS29300"
     CDS             complement(join(4946..5049,1..43))
                     /locus_tag="L6451_RS29300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796009.1"
                     /translation="MKKTKKQAKKQPKKQPKKQMKTAMKIVGITKDVVNFVGNLIRIYT
                     FFF"
                     /name="hypothetical protein"
     gene            912..1064
                     /locus_tag="L6451_RS29305"
                     /old_locus_tag="BCM0105_p51"
     CDS             912..1064
                     /locus_tag="L6451_RS29305"
                     /old_locus_tag="BCM0105_p51"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796023.1"
                     /translation="MPKVVSSKEKMQPKTFKLHPRTIEQIKELAEKKQMTQAEVIDKAL
                     KLLAR"
                     /name="hypothetical protein"
     gene            1157..1879
                     /locus_tag="L6451_RS29310"
                     /old_locus_tag="BCM0105_p52"
     CDS             1157..1879
                     /locus_tag="L6451_RS29310"
                     /old_locus_tag="BCM0105_p52"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236796021.1"
                     /translation="MKKAFAENYINPVVERFSELDTPNGGFYTSIPQAVQYCWFLDDKE
                     LSVLLALSTYADENGRCKVYQRTLARKVKIKSTTTLRTKLKSLVKKRFILQVNTDGESS
                     IYQIKDYSTNPYILLSEAIEKVEQVAKKKYGVSVSKIAETMDELIASKIDYMAMITKIE
                     ESFNGYDVALKSVQGYVNEALGLEMDISLQTKKPRRERGFKMSKVIKPKQPKRNREFII
                     SEGESEAPVKREGLRLRL"
                     /name="helix-turn-helix domain-containing protein"
     gene            2082..2927
                     /locus_tag="L6451_RS29315"
                     /old_locus_tag="BCM0105_p53"
     CDS             2082..2927
                     /locus_tag="L6451_RS29315"
                     /old_locus_tag="BCM0105_p53"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_236796018.1"
                     /translation="METDISIDRLTLVGYDTGMIEGYLKYSEYISFVGFSKNYGFRHSY
                     QGIKGELLEIGEKGKVRLDFNPNTANMEQVRDVVSRLKYIYLTRLDIAIDYFGVDLSEV
                     EWTSPKRRKQNLWLDPEGQQETRYIGAPSSERRYRIYNKKLERLEKKEELDPRAVNGQW
                     RVEVQQRFKESNNILDPIDYFTGDLFDIRPYRKVVDLSHIEKPMERIIIAGLMARPSEL
                     KNLSKNARGKYKKMIQEAREKSGVAINPEPIEVYTKEKDLLAEQLTSLFAHCAKSVAQV
                     "
                     /name="replication initiation factor domain-containing
                     protein"
     gene            3345..3668
                     /locus_tag="L6451_RS29320"
                     /old_locus_tag="BCM0105_p54"
     CDS             3345..3668
                     /locus_tag="L6451_RS29320"
                     /old_locus_tag="BCM0105_p54"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796016.1"
                     /translation="MMMNLLSLQQEANSVIQDLNGKDISSMSLIQKANYSNRIVKVRMS
                     LEAYRNTPGVNEFINALDHLRFKLAEVQQQETRQQANKRRAEIIQNGIKETPYPTMPMF
                     LNN"
                     /name="hypothetical protein"
     gene            3680..4018
                     /locus_tag="L6451_RS29325"
                     /old_locus_tag="BCM0105_p55"
     CDS             3680..4018
                     /locus_tag="L6451_RS29325"
                     /old_locus_tag="BCM0105_p55"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="WP_236796014.1"
                     /translation="MSDNVNFPLRIPRELRDKFKKVSQKTGTSMNSSICQLIAEHIYKM
                     EQLGLKDIEEPNVIQVTPVKEETKAFIKKAIKEADEAKELAETKTKKRLRKSPKKKIGG
                     ETDGSKKD"
                     /name="Arc family DNA-binding protein"
     gene            3999..4238
                     /locus_tag="L6451_RS29330"
                     /old_locus_tag="BCM0105_p56"
     CDS             3999..4238
                     /locus_tag="L6451_RS29330"
                     /old_locus_tag="BCM0105_p56"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796012.1"
                     /translation="MEVKKIKIKPKKIVRNEENHKLEFDKVRLSIEIPISIRDNFKEAV
                     EKNGKTMKEVLEAYMQRYSVKELLTDLKEETIDN"
                     /name="hypothetical protein"
     gene            4722..4922
                     /locus_tag="L6451_RS29335"
                     /old_locus_tag="BCM0105_p57"
     CDS             4722..4922
                     /locus_tag="L6451_RS29335"
                     /old_locus_tag="BCM0105_p57"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796011.1"
                     /translation="MDIEAKKEAFVTFLVEKVLFQTFAERTTSLEEKEVYEKFIKLYDQ
                     ELNRLNFELTIASVNRKRANL"
                     /name="hypothetical protein"
ORIGIN
        1 gtttcttagg ttgttttttt gcttgttttt tagttttctt cataaattaa ttccccctat
       61 ttagtagttg ttcgtcttgc tatctattac ttgtgacgtt ttttgagaaa tccttatttt
      121 ttttattctt tttcaaaaaa atttctttta taaacatagg cgcagatact aaggtttttt
      181 ggtttgctat atgtataaaa cttgagtaaa aatatcaatt tggattgtca atggaataac
      241 acctcctagt tttctattcg tcttttcatc tatgttttgt gacacttttt gagagatttg
      301 tattaagagt ttttttcttc aaagagatag gcacattata ttgttcgtgt tggtgtgatt
      361 cttttcttag ttaaatttta gagcttttct tcatagtatt tatccttctc tttatcctta
      421 atttatccgt gatttatccg tctttttatc gagattatcc tctatagaaa agtgtatctt
      481 ggataaagat tgaaaaaggt atctaattta gtagcccaaa tagtagctca aaagtgccat
      541 tactctttct atacaaaaca ataacgattt ttcatttgaa aatagtctcc caaaaagttt
      601 gggttttgtt cggatgatta agaactatgt attctagttc ttgctctgat tttttataac
      661 tatagggatg ttcgacacgt cgaaaaaccc gcttctctaa cagcgggtta gcgtcctctt
      721 tcgagggatt caccatatca aaatgttttt tgttttaggt tttctccttt cttttttgat
      781 caaaaaaatg tccttattag tttgttctat ttttttatgt gttttttgac tgtttttgaa
      841 gtggacaaat agaatcgttg atgttaagct gtttttaggg agtacctatc attgtgttga
      901 ggagtgatat tatgccgaaa gtagttagtt ctaaggaaaa gatgcaaccc aaaacattta
      961 aattgcatcc taggacgatt gaacaaataa aggaattagc tgaaaagaag caaatgacgc
     1021 aagctgaagt gattgataag gcgttaaagt tattagcacg ataaaaaaat atatatccca
     1081 tcgcaaataa cgtagcatac taacatagca aacaagataa caaggaggga aaattaaaag
     1141 gaaaaggtga tctgtaatga aaaaggcatt tgcagagaat tatattaatc ctgttgtgga
     1201 acgttttagt gaattagata ctccaaatgg cggtttctat acttccattc cacaagcagt
     1261 tcaatactgt tggtttttag atgataaaga gttaagtgta ttattagcac tatcgaccta
     1321 tgcagacgaa aatggacgtt gcaaggtata tcaacgaacg ttagctagga aagttaagat
     1381 taaaagtaca acaacattac gaactaaatt gaaaagttta gtgaagaagc gttttatatt
     1441 gcaggttaat acagatggag aatctagtat ttatcagatt aaagattatt ctaccaatcc
     1501 ttatattctt cttagtgagg ctattgaaaa agtcgaacag gtagctaaaa agaaatatgg
     1561 tgtttcagta tctaaaatag ctgaaacgat ggatgaatta attgctagta agatagatta
     1621 tatggctatg ataactaaga tagaggaaag ttttaatggt tatgatgtag ctttaaaatc
     1681 tgtacaaggt tatgtaaatg aggctttagg attagaaatg gatatttctt tacaaacgaa
     1741 aaaacctcga cgggaacgag gttttaagat gagtaaagtt attaaaccta agcaacctaa
     1801 acgtaacaga gaatttatta tttcagaggg tgaatctgaa gctcctgtga aacgggaagg
     1861 tcttagatta cgactttgaa taaggtaatt atatattacc ttataggact tgaataccta
     1921 gtcaaaaatt gattacccct tacacaattt ttgtgtacct cttaatcaaa atttgattac
     1981 tactaaatat aaaatactaa aaagaaatta atatatatat ctatcaaaga aattactgaa
     2041 gcatactaag atagcaaata aaacggaaat gagtgtttag gatggaaaca gatattagta
     2101 tagaccgcct aactctcgtt ggatacgata ctggaatgat agaagggtat cttaaatact
     2161 cggaatatat ttcattcgtg gggttttcta agaattatgg ttttcgtcat tcataccaag
     2221 ggataaaagg agaattgctt gagataggcg agaaagggaa ggtacgttta gattttaacc
     2281 ctaataccgc aaatatggag caggtaagag acgtcgtttc tcgactgaaa tacatctatt
     2341 taacgcgatt agatatagcg atagattatt ttggtgtaga tttatctgag gttgagtgga
     2401 caagccctaa acgaagaaaa caaaacttat ggttagaccc agaagggcaa caagaaacga
     2461 gatacatagg tgcgccatca agtgagagaa ggtatcgtat ttataacaag aagttagaac
     2521 ggttagaaaa gaaagaagag ttagacccta gagcagttaa tggacagtgg cgtgttgagg
     2581 tacaacaacg ttttaaagag tccaataaca tattagaccc tattgattat tttacaggtg
     2641 atctattcga tattagacca tatcgaaagg tggttgattt atctcatata gaaaagccta
     2701 tggaacgaat tataatagcg ggattaatgg caagacctag tgaacttaag aatcttagta
     2761 agaacgctag aggtaagtat aagaagatga tacaagaggc tagggaaaaa agcggtgtag
     2821 cgataaatcc tgaaccaata gaagtttata caaaagaaaa agacttgtta gctgagcaac
     2881 taacaagcct gttcgcccac tgtgctaaga gcgtagcaca agtgtaaact ttaaaactta
     2941 taacaagtaa atatctaatg ttagtatagc aaaagaagag agggaattca cctctctttt
     3001 tctttgtttt aaggggattc gttaagaata tatgtatttc ctttaagttt attgcgttag
     3061 ctcgtgcttt aggattaata gttgtctatt taggctattg tttgaaaaac accccaccca
     3121 ccgcttatta ggttctgtat ctgaaaatgc gctcctacac cctctcgatt ttgttaattt
     3181 ttatataggt aaggtgacaa tttaaaaata aattttaaat tttttaagta acccagtata
     3241 cagatttttg gttaagagtt acgatgtttt tataaatgac tatcatattg ataatcaatt
     3301 taatagtcgt attgattatc acaacagaaa agagggaaaa tattatgatg atgaatttgt
     3361 taagtttaca acaggaagct aacagtgtga ttcaagattt aaacggtaag gatatttctt
     3421 caatgagttt gatccagaag gctaattatt ctaatcgaat agtgaaagtt agaatgagcc
     3481 ttgaggcata tagaaatact ccgggagtaa atgaatttat aaatgcattg gaccacttaa
     3541 gatttaagtt agctgaagta caacaacagg aaacacgaca acaggctaat aaaagaagag
     3601 ctgaaataat tcaaaatgga ataaaagaaa caccgtaccc aacgatgcca atgtttctaa
     3661 ataattagga ggataggata tgagcgataa tgttaatttt ccattgagaa tacctcgtga
     3721 attgcgggat aagtttaaaa aggtttcaca gaagactggg acgagtatga actcatcaat
     3781 ttgccaacta atagcggaac acatttataa aatggagcaa ttaggattga aggatataga
     3841 agaacctaat gtaattcaag ttacgccggt taaagaagaa acaaaggcgt ttataaagaa
     3901 ggctataaaa gaagctgatg aagctaagga attagctgaa actaagacga aaaaaagatt
     3961 aagaaagtcg cccaagaaga aaataggcgg tgaaacagat ggaagtaaaa aagattaaga
     4021 tcaaacctaa aaaaatagtg cgtaatgaag aaaatcataa attagaattt gataaggtaa
     4081 gattaagcat tgaaatccct atttctatta gagataactt taaagaagcg gtagaaaaga
     4141 acgggaagac gatgaaggaa gtattagaag catatatgca aaggtatagt gttaaagagc
     4201 tattaacgga tttaaaagaa gaaacaatag ataattagaa tctgaatacc tcgtttaaac
     4261 cttgaataca tgtacatttt taaggcttaa attttatagt tggtaaaagt agaaaaaaca
     4321 cgacctatca ataaaaatat cgctaaattt acgctaaaca actataagta ttcttcctaa
     4381 gatagtggtg aaccaggagg cattcatgtg agggcatagt ctttgcggtt tacggtatgt
     4441 cggtttttag ggaagaaatg gcgaggttta acgaggattg gcgaagaatt aagggttaaa
     4501 ttaattggtt ttcatcgagt attagcgaag attagggaag accactaagg aatcaaagcg
     4561 tagcggttca tttacaatga gaatgtccgt ggtagcctgt tatggcttgc caatccttgt
     4621 ttgagcgtta gcgagttgaa ggagtggggc attggcttat gacccacgga ctggaggctc
     4681 gttgtcaatg attgaattcc gcttattgga ggaagtaagt tatggatata gaagctaaga
     4741 aagaagcatt tgtgaccttc cttgtagaga aagttctatt tcaaacattt gcagaaagga
     4801 ctacttcttt agaagaaaaa gaggtgtatg aaaagtttat taagctatat gatcaagaat
     4861 taaatagact aaatttcgaa ttgacaatag catctgtaaa tagaaaaaga gccaacctat
     4921 aatgttatag attagctctt tttgtttaga agaagaatgt ataaattcgg attagatttc
     4981 caacaaaatt gaccacgtct tttgttattc cgactatttt cattgctgtc ttcatttgtt
     5041 tcttaggtt
//
