LOCUS       NZ_AP022918             8313 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0105 plasmid pMRY14-0105_3, complete
            sequence.
ACCESSION   NZ_AP022918
VERSION     NZ_AP022918.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203329
            Sequence Read Archive: DRR206369, DRR206393
            Assembly: GCF_021655015.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 354x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655015.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:26:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,896
            CDSs (total)                      :: 5,744
            Genes (coding)                    :: 5,528
            CDSs (with protein)               :: 5,528
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 95 of 216
            Pseudo Genes (incomplete)         :: 131 of 216
            Pseudo Genes (internal stop)      :: 48 of 216
            Pseudo Genes (multiple problems)  :: 51 of 216
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022918.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0105"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0105_3"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            join(7763..8313,1..49)
                     /locus_tag="L6451_RS29195"
     CDS             join(7763..8313,1..49)
                     /locus_tag="L6451_RS29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562260.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_236788723.1"
                     /translation="MNKRFAYIRVSDKTQNENRQLDEMLQQEIEERDIFIDKASGKDFN
                     RPKYQALKQMLRAGDILYIKSIDRFGRNKDDILKEWNEITKEIGADIVVLDMPLLDTRQ
                     YKDVLGSFVSDLVLQILSFFAEEERRKIKERQREGIDAAQRRGAKFGRPAATFPPGFEE
                     VYKRWKDGEIKAIEAMEELELKRTTFYKLVNSFENK"
                     /name="recombinase family protein"
     gene            800..997
                     /locus_tag="L6451_RS29200"
                     /old_locus_tag="BCM0105_p301"
     CDS             800..997
                     /locus_tag="L6451_RS29200"
                     /old_locus_tag="BCM0105_p301"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788725.1"
                     /translation="MIGKDMITLDNPKKEYFQQYAQNNKEQINANARRRRQQQKEKVEK
                     YDRLVAALRSRGISVEELLK"
                     /name="hypothetical protein"
     gene            1420..2520
                     /locus_tag="L6451_RS29205"
                     /old_locus_tag="BCM0105_p302"
     CDS             1420..2520
                     /locus_tag="L6451_RS29205"
                     /old_locus_tag="BCM0105_p302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205124.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_098492605.1"
                     /translation="MNHEEFINSTEIDDKFLKQIIDNREFNDVIVSYYERLHKESRDDG
                     MKKKQENVKGCNDFWIIDKYHEHRLKEFKRTNLCKDKFCNNCKKVKQASRMGKFIPLIQ
                     PYAENMYQLTLTIPNVKGDELKDTLSKMQKAFARLIEYFKGKKKINGIDFEVLQYEGAI
                     RSLEITFNGNEYHPHYHALIVLKVNKFHDCMIRKHKNVYSIDFTGKREARLFSDIEVLI
                     QKVWYLIINGKRITETAIQSLKKGYSCQLDKFSENDFVEIFKYMTKATTEDGKTLSYKQ
                     FKTLYYALFKVRQIQGYGIFYNLKDDEEINFDEVNEAYAEFIEELQTKEKPVTVSEAPE
                     QVRKESDYTVFSRKTFQKYWKQVMSE"
                     /name="protein rep"
     gene            2988..3137
                     /locus_tag="L6451_RS29210"
                     /old_locus_tag="BCM0105_p303"
     CDS             2988..3137
                     /locus_tag="L6451_RS29210"
                     /old_locus_tag="BCM0105_p303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788727.1"
                     /translation="MEREKCSLKEAKNAYNRKWRAANRDKVKLYNDRYWEKKAKEMEEQ
                     ACQK"
                     /name="hypothetical protein"
     gene            3125..3397
                     /locus_tag="L6451_RS29215"
                     /old_locus_tag="BCM0105_p304"
     CDS             3125..3397
                     /locus_tag="L6451_RS29215"
                     /old_locus_tag="BCM0105_p304"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788729.1"
                     /translation="MSEVSMETVIKGKQQSELLKHLEKVGIELLGRRDELLEQWEKEGL
                     KEDSIFEDDLKFVEELMNRNDELMFDVKVELITTMDEIHHQKMGY"
                     /name="hypothetical protein"
     gene            3402..3653
                     /locus_tag="L6451_RS29220"
                     /old_locus_tag="BCM0105_p305"
     CDS             3402..3653
                     /locus_tag="L6451_RS29220"
                     /old_locus_tag="BCM0105_p305"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788731.1"
                     /translation="MLGKYDGLLEKERLLKRIDELSFALEASGDPMLEAMVKNSGNTDI
                     AVAKELITKGSELGMNEHVFKKALLAKVNELREEVTKL"
                     /name="hypothetical protein"
     gene            3650..4063
                     /locus_tag="L6451_RS29225"
                     /old_locus_tag="BCM0105_p306"
     CDS             3650..4063
                     /locus_tag="L6451_RS29225"
                     /old_locus_tag="BCM0105_p306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788733.1"
                     /translation="MKKDKQKDLPFNHKVVLSVLPLGRSKAITTKEIMARSGIKDKRAV
                     SAILSELVTVYKQPIATTSKKEFKGVFIVQDVIDYQIGKEGLVSRKRHIDKRDSAYTEA
                     CLEIFKGTVTLDESKNKVEGSVRACKQSASSSM"
                     /name="hypothetical protein"
     gene            4033..4740
                     /locus_tag="L6451_RS29230"
                     /old_locus_tag="BCM0105_p307"
     CDS             4033..4740
                     /locus_tag="L6451_RS29230"
                     /old_locus_tag="BCM0105_p307"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788735.1"
                     /translation="MQTISKFFNVDNESKLNELTIAKGELNSKESELQSKLSRVERALE
                     LAQTDLMIDDSTANKKVVAKYEEAQEKFQSELIATQEELNKVSDEIAAIVKEELHAELV
                     DAAKQDVMLIELPEKCRKAKKVINRDLYRATSFEDEGRFSRKYLHVSATVIDDARTFAD
                     IHRFADKPNLTRIGYQRGELIRWGGDNNLFKLNDEYETIYEEEAQKIKDKTDKEYEQFM
                     KAFNKYFAVSDSH"
                     /name="hypothetical protein"
     gene            4794..5219
                     /locus_tag="L6451_RS29235"
                     /old_locus_tag="BCM0105_p308"
     CDS             4794..5219
                     /locus_tag="L6451_RS29235"
                     /old_locus_tag="BCM0105_p308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788712.1"
                     /translation="MSKDTLKNIFHVYCFYTVRLQGDTEPRMRRNKLNYDNPIQNYYES
                     FIDCLRGFCEVRSDVSLYSFYQFVVDAVNSLNKQERKLIYERYLYKDHYKSDRQHYLAM
                     DITPQNYKKQMDPVRRKLIKNLGIEDLQLNIPDWMKR"
                     /name="hypothetical protein"
     gene            5350..5598
                     /locus_tag="L6451_RS29240"
                     /old_locus_tag="BCM0105_p309"
     CDS             5350..5598
                     /locus_tag="L6451_RS29240"
                     /old_locus_tag="BCM0105_p309"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236788716.1"
                     /translation="MKSLYRILELDKDEVSSSEKLILLTIAIYSDNKFIPFSITELENY
                     TNLSRATVTRLVKQLEEKGFVEVQRNGTATNNYKVTL"
                     /name="helix-turn-helix domain-containing protein"
     gene            5675..5851
                     /locus_tag="L6451_RS29245"
                     /old_locus_tag="BCM0105_p310"
     CDS             5675..5851
                     /locus_tag="L6451_RS29245"
                     /old_locus_tag="BCM0105_p310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176543923.1"
                     /translation="MLPQKEQLGHLAEKTELVKDLAWIAHDLLTEEDYTKENAAEALIK
                     VINRELSYVSKVR"
                     /name="hypothetical protein"
     gene            5893..6099
                     /locus_tag="L6451_RS29250"
                     /old_locus_tag="BCM0105_p311"
     CDS             5893..6099
                     /locus_tag="L6451_RS29250"
                     /old_locus_tag="BCM0105_p311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788718.1"
                     /translation="MANTVAKDKFEIVEKFVNACMLKGVSEDKSFKLVEELCTLIQQKR
                     HDEWFGLVDEALKEAGHEEGVTL"
                     /name="hypothetical protein"
     gene            6096..6227
                     /locus_tag="L6451_RS29480"
                     /old_locus_tag="BCM0105_p312"
     CDS             6096..6227
                     /locus_tag="L6451_RS29480"
                     /old_locus_tag="BCM0105_p312"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272880692.1"
                     /translation="MSEVEMVLHEFEELLHKIISAAQHGDEQAMNHLDIVKAWLKDN"
                     /name="hypothetical protein"
     gene            6304..6543
                     /locus_tag="L6451_RS29255"
                     /old_locus_tag="BCM0105_p313"
     CDS             6304..6543
                     /locus_tag="L6451_RS29255"
                     /old_locus_tag="BCM0105_p313"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788720.1"
                     /translation="MAEEHRYHVTFYFSNGKEFDGKVTSKYNKEDYLEGLEDLFLKEKT
                     LLIDHLGMLIQTKYITHVKVIEVGSKIGADKKDT"
                     /name="hypothetical protein"
     gene            6604..7140
                     /locus_tag="L6451_RS29260"
                     /old_locus_tag="BCM0105_p314"
     CDS             6604..7140
                     /locus_tag="L6451_RS29260"
                     /old_locus_tag="BCM0105_p314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_236788722.1"
                     /translation="MTDKTDSSQSIYISKDVAMMLKIQESTLRKYCIMLEEHGYKFHKN
                     EHGHRGFLDNDVITLRKLIEIKSHPDMTLKQACNAIMTWVKEKDMSEVDTNVITENEQH
                     DERYSELKEMIQQQNELLKKMAKKLDDQQRYIDEKLEKRDQQLMGAIREMQEEKRVLLE
                     TASASQEKKWYKFWK"
                     /name="DUF3967 domain-containing protein"
ORIGIN
        1 aaaacggact actttctata aactagtaaa ttcattcgaa aataagtaaa aaagtaataa
       61 taatattaaa ctttttacaa aattaagtcg aaaaagcaaa gttaaaaaag acaaacatac
      121 cgataacaag cattgatatt acacataatt ttatgccttt catgtcgaaa agtaaaagtt
      181 aaaagtaata aaaaagcaaa gtataaaagt atacttttaa gtttgcttgt ttctatgaaa
      241 atccgttggt ataatagttt taacgtgttt gagtgtttca aacgaaaaga aaaaagccga
      301 gttggggaac tcgacttaag tctttctaaa agtatgggga tacttttaga ttagattttg
      361 tgtctcgaca acactttcga ctaattagca ctaccaatgc ttattagtca ttgatgattt
      421 tacgaatcat cttaacgatt atttttttat ggattgacac ctattttatc gacctttgac
      481 aatcaaataa ttaggtacca tttatatcta agattgaatt aacaacctta tttaacgtat
      541 gaaaaagtac tgtaggtcaa gttgtaaact ataaaaagtt tcttcttagg aaaccttaag
      601 agaaaaaaat atagatacaa tttgtatcgc tttaatacga aaaaaagttc tcgttcagcg
      661 aacttcctat gtgacaagtt tacaatgtat ttttcgtttt gacaatactt taagtaaact
      721 catagttttt acatacattg aaaggtacct ctactatttg aggtgccttt tttatttatt
      781 tttgaaagga tgatagaaaa tgatagggaa agatatgatc acattagaca acccaaagaa
      841 agagtatttt cagcaatatg cacaaaataa taaagaacaa attaacgcca atgcaagaag
      901 acgaagacaa caacaaaaag aaaaggtaga gaagtatgat agattggttg cggcgttacg
      961 atctagagga attagtgtag aagaattatt gaaataagat ggacaagctg tattaaggcc
     1021 ggactaagac accaggagaa gatttgtata cggtctaggt tattcgtggg gcttatgcta
     1081 cagctgtcat tgtctagcgg tatcgcagtg cgtaagcacg agaataagga gcagacatac
     1141 accacaagcc ccgaacactt tcatgctctc caaaacaaca tgttttgggg atacctagtg
     1201 taacgtggcc gagtgcaacg gaaggtttta atgtttcgtt taaaaaatga gtttcttcta
     1261 ttacttgata tattagaaat aagtcggcta tttctctagt tttctagtaa tgacaaattg
     1321 tcgataacca tagtcatatc aagggtttaa gctattcgac ttattgctaa taaggcttaa
     1381 aaagccttcc aaaaattcca ctagaaatga ggtgagaaat tgaatcatga ggaatttata
     1441 aattcaactg aaatagatga taaattttta aagcaaatca ttgataatag ggaatttaat
     1501 gacgttatag tctcgtatta tgaacgacta cataaagaat cacgcgatga tggaatgaag
     1561 aagaagcagg aaaacgttaa aggttgcaat gatttttgga ttatagacaa atatcatgaa
     1621 catagattaa aagagtttaa acgaactaat ctttgcaagg acaagttttg taacaactgt
     1681 aaaaaggtta aacaagcttc acgcatgggt aaattcatac cacttattca gccttacgcg
     1741 gaaaatatgt atcaattaac gctaactatt cctaatgtta aaggcgatga attgaaagat
     1801 acactaagca agatgcaaaa agcttttgct cgtttaattg agtatttcaa aggcaaaaag
     1861 aaaataaacg gaattgattt tgaagtttta caatacgaag gcgctatacg atctttagaa
     1921 ataacattta acggtaatga gtatcatcca cattatcatg ctcttattgt tttaaaggtt
     1981 aataaattcc atgattgcat gataagaaaa cataaaaatg tttattcaat agacttcact
     2041 ggaaaacgtg aggcgcgttt atttagtgat atagaggttt tgatacagaa ggtttggtac
     2101 ttgattataa acggtaaacg gataacagaa acagcgatac agtcacttaa aaaaggttat
     2161 tcatgtcagt tggacaagtt tagcgaaaac gattttgtag agatatttaa gtacatgaca
     2221 aaagcaacaa cggaagacgg taaaacattg agttataagc aattcaaaac gttgtattat
     2281 gcgttattta aagttagaca aatacaaggg tatggcatct tttataattt gaaggatgat
     2341 gaagaaatta attttgatga agttaatgaa gcgtatgcgg aattcataga ggaattacag
     2401 acaaaagaaa aacctgttac ggtatcagaa gcaccagaac aggtcagaaa agaaagtgac
     2461 tatacagtgt ttagtcgaaa aacgttccaa aaatattgga agcaagtcat gagcgaatag
     2521 ccttactcat tccggcaagt ttgataaaag gctatcttta ccaccattaa tcaaataacg
     2581 gagggttttc taatatcatt atacataatt ttaattcgta tgatattagg aaattccttc
     2641 tatatcgtta attagtagag actatgaatt atttcgtagt ctttttttat tggaaaggaa
     2701 tgattgttgt tatatttaga ggtttataac aggaaaaagg cgcctgatct cgtttgaatc
     2761 agaacgaaat caagcgcctt tgggcgataa ttaaccaaga acaagttaat taaataaatc
     2821 taaaagtttt tggaagtact tatattatat cgtatatgga gttcccggac agtaatttta
     2881 gcctaaattt gcgtgaagca aatcattaat cataccacgt aaactatata ttcgtaaata
     2941 ggtaaatgtt aaaataagag taagaagaat gaaaagaagg tggaaaagtg gaaagagaaa
     3001 agtgttcttt gaaagaagct aaaaacgctt ataaccgaaa atggagagca gcgaatcgcg
     3061 ataaagtgaa gctgtataac gatagatact gggaaaagaa agctaaagaa atggaggaac
     3121 aagcatgtca gaagtaagta tggaaactgt tattaaagga aagcagcaga gtgaactgtt
     3181 gaagcattta gaaaaagtag gtattgaatt actggggcgt agagatgaat tgttagagca
     3241 gtgggaaaaa gaaggactta aagaagacag catttttgaa gatgatctta aattcgttga
     3301 agaattaatg aaccgaaatg acgaattaat gtttgatgta aaagtggaat taattacaac
     3361 tatggatgag attcatcatc agaaaatggg gtattgatag gatgttagga aagtatgatg
     3421 gattattaga aaaagaacgg ttacttaaac gaattgatga actgtcattc gcgttagaag
     3481 cttcaggtga ccctatgttg gaagcgatgg ttaaaaatag tggcaataca gacatcgcgg
     3541 ttgcaaaaga gttgattaca aaagggagtg aattaggtat gaatgagcac gtatttaaaa
     3601 aagcgttgct agcaaaagta aatgaacttc gtgaagaggt aacgaaacta tgaaaaaaga
     3661 caaacaaaaa gacctccctt ttaatcacaa agtagtattg agcgtactac ctttaggaag
     3721 atctaaagca ataacaacta aagaaattat ggcacgtagt ggaataaaag acaagcgtgc
     3781 agtatctgct attttaagtg aattagtgac tgtatataaa caacctattg ctacaacgtc
     3841 taaaaaagaa tttaagggcg tttttattgt acaggatgta atagattatc agattggtaa
     3901 agaaggcctt gtgagcagaa aaaggcatat agataagcgt gattcagctt atacagaagc
     3961 gtgcttggaa atattcaaag gtacagtgac attagatgag tcaaaaaaca aagtggaagg
     4021 aagtgttcgt gcatgcaaac aatcagcaag ttcttcaatg tagataacga gagtaagtta
     4081 aacgaattaa caatagcaaa aggggaattg aattccaaag aatcagaatt acaaagtaag
     4141 ctatctcgag tagaaagagc tctggaactg gcacaaactg acttaatgat tgatgatagt
     4201 acagctaata aaaaggttgt tgcaaaatat gaagaagctc aagagaagtt ccaatcagag
     4261 ttaatagcta ctcaagaaga acttaacaag gtttctgatg agattgcagc tatcgttaag
     4321 gaagaattgc atgctgaatt agtagatgcc gctaaacagg acgttatgct aatcgaacta
     4381 cctgaaaagt gcagaaaagc gaaaaaagta attaacaggg atctttatag ggcgacgtcc
     4441 tttgaagatg aaggtcgatt tagtagaaag tatttgcatg ttagtgctac agtgatcgac
     4501 gacgctcgta ctttcgccga tatacatcgt tttgctgaca aaccaaatct aacacgaata
     4561 gggtatcaac gcggtgaatt gatacgttgg ggtggcgata ataacctgtt caaattgaat
     4621 gatgaatatg aaacgatata tgaagaagaa gcacagaaaa tcaaagacaa aacggataaa
     4681 gagtatgagc agttcatgaa ggctttcaat aagtatttcg ctgtgtcgga ttctcattaa
     4741 tcaaaataca taagtcgcct aagggcggct tttttttatt aggagggatt ttcatgagta
     4801 aagatacatt aaaaaacatc tttcacgtgt attgttttta tacagtacgt ttgcaaggcg
     4861 atacagaacc acgtatgaga cgaaacaagc taaattatga taatcctata caaaactatt
     4921 atgaaagttt tatagactgt ctgagaggat tctgtgaggt taggagcgac gtttcattgt
     4981 attcgttcta tcaatttgtt gtagatgctg ttaattcgtt aaataagcag gaaagaaagc
     5041 tgatctatga gcggtattta tataaagatc actacaaatc agataggcaa cattaccttg
     5101 cgatggatat aacaccacag aattataaaa aacagatgga tccggtaaga aggaagttaa
     5161 ttaagaacct cggaatagaa gatttacagt tgaatattcc agattggatg aaacgataac
     5221 gggttaaatt acatgtatgg ttatattatt tttaaatttt attttgattc tattgcattt
     5281 tactatactc attgttatac tagataatgt aaacaacatg ttgatttata ggaggttact
     5341 agtttaatta tgaaatcact atatcgtatt cttgaattgg acaaagatga agtttcttct
     5401 tctgaaaaac taatcttgct aacgatagcg atctattcag acaacaagtt tatcccattc
     5461 tctattacag aattggagaa ttacacgaat ttgagtagag ctacggttac aaggctcgta
     5521 aaacagttag aagaaaaggg attcgtcgag gttcaaagga atggaacagc aacaaacaat
     5581 tacaaagtaa cactgtaaaa acaaagaaag agcaggagat tgcggctcct actctttcaa
     5641 aacacattat cccttatgga aaggatgtat tagtatgtta ccacaaaaag aacagttagg
     5701 acatcttgct gagaaaactg aattagttaa ggatttagct tggattgcac atgatttact
     5761 taccgaggaa gattacacca aagagaatgc agcggaagcg ttaattaagg tcattaatag
     5821 ggaattatca tacgtttcca aagtgcgtta aacgggatta tatgtaaata aaacaattcg
     5881 gaggtaatat tcatggcaaa tacagttgct aaagataaat ttgagatcgt agaaaagttt
     5941 gtgaatgctt gtatgttaaa aggtgtttcg gaagataaat cattcaaatt agtagaggaa
     6001 ttatgtacac ttattcaaca aaaacgtcat gatgaatggt ttggattagt agatgaagca
     6061 cttaaagaag cgggacatga agaaggtgta acgttatgag tgaagtagaa atggttttac
     6121 acgaattcga agaactatta cataaaatca tatcagcagc gcaacatggc gatgaacaag
     6181 cgatgaatca tttagatata gtaaaagcat ggttaaagga caattaaggc gtggtgatcc
     6241 acgtcttttt tttatactgt cttggggaat cctactgata tattttgaaa gtaggtggac
     6301 taaatggctg aagaacatag ataccatgta actttttatt tttctaacgg aaaagaattt
     6361 gatggtaagg taacaagcaa atacaacaaa gaagattatt tagaagggtt agaagatcta
     6421 ttccttaaag aaaagacatt actgattgat catctaggaa tgttaataca aaccaaatat
     6481 attacacatg tcaaagttat cgaagttgga tctaaaatag gagcagacaa aaaagatacg
     6541 tgaattatta aggaaaagga tatatcattg ttataacgaa tgttttggta gaggtgaagc
     6601 gttatgacag ataaaacaga cagctctcaa tccatatata taagcaaaga cgtagcgatg
     6661 atgcttaaaa ttcaagaatc gactttacgt aaatattgta tcatgcttga ggaacacggc
     6721 tacaaatttc ataagaatga gcacggccat agaggattcc ttgataacga tgttataacg
     6781 ttaagaaaat taatcgagat taaatcacat cctgatatga cattaaaaca ggcctgtaac
     6841 gcaattatga cgtgggttaa agagaaagat atgtcagagg ttgatacgaa tgttataacg
     6901 gaaaatgagc aacatgacga gcgatatagt gagcttaaag aaatgattca gcagcagaat
     6961 gaattattaa agaaaatggc gaagaaatta gatgatcagc agaggtatat tgatgaaaag
     7021 ttagaaaaac gagatcagca gttaatggga gctatccgag aaatgcaaga agaaaaacga
     7081 gttttgttag aaacggcttc cgctagtcaa gaaaagaaat ggtacaagtt ttggaagtag
     7141 gattccctaa ctatggggag tcctttttta ttttaaagtg agtttggatc acttagaggt
     7201 agattaaaca gaaagccctg caggtaacat cctcagaaac gcacaagaaa agtggtgcgt
     7261 ataactgggc actcgctttt ttctcgtgaa taagatctca ttgaatggag taattttgta
     7321 tactactccg ttggaatata gaacgtttta cggagcgttc caatatcgtt acctttgcaa
     7381 aacaaacacg aataccacgt tactttaatc cgcacacaag gcaccctttt tatgcagtac
     7441 tcattgtagt attgcacccc ttttaatgtt gtactgcaaa acaaacacaa ctcaactaat
     7501 aaagaacaaa gcacatatac cggtaattga atggtaacgg gtgttaagga atgataagga
     7561 gtattagtgc aaaatgaatg tagtagtatt ttagtaattt gcaaaacaaa cgaactcaat
     7621 taatgtattc gtaaaagtac acatttacga ataagaaaaa gaataagaaa agatattgtt
     7681 ttgtgttcgt ataagttgta tgattagttt acaaacattc gtaaattgaa aaacactttt
     7741 acgaacaaag gggaagagga aaatgaacaa acgtttcgct tatatccgtg tatcagataa
     7801 aactcaaaac gaaaatagac agttggacga gatgttgcag caggagattg aagaaagaga
     7861 tatcttcatt gacaaggcaa gcggtaaaga ttttaatcgt cctaaatacc aggctttaaa
     7921 acagatgcta cgtgccggag atatactgta tatcaaatct attgatcgtt ttggtagaaa
     7981 taaagatgat attttaaaag agtggaatga aataacgaag gagatcggtg cggatatcgt
     8041 tgtattggac atgcctttat tagatacaag acaatataaa gatgtattag gctcgtttgt
     8101 aagtgatttg gtactacaga tactatcatt ctttgcagaa gaagaaagac ggaaaatcaa
     8161 agagagacaa cgtgagggca ttgatgccgc acaaagacga ggtgcgaagt tcggaagacc
     8221 ggctgctaca ttcccgccag gattcgaaga agtgtacaag cgttggaaag atggagaaat
     8281 taaagcaata gaagctatgg aagaattaga gtt
//
