LOCUS       NZ_AP022917             8315 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0105 plasmid pMRY14-0105_2, complete
            sequence.
ACCESSION   NZ_AP022917
VERSION     NZ_AP022917.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203329
            Sequence Read Archive: DRR206369, DRR206393
            Assembly: GCF_021655015.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8315)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 354x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021655015.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:26:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,896
            CDSs (total)                      :: 5,744
            Genes (coding)                    :: 5,528
            CDSs (with protein)               :: 5,528
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 95 of 216
            Pseudo Genes (incomplete)         :: 131 of 216
            Pseudo Genes (internal stop)      :: 48 of 216
            Pseudo Genes (multiple problems)  :: 51 of 216
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022917.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8315
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0105"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0105_2"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            join(8181..8315,1..15)
                     /locus_tag="L6451_RS29125"
     CDS             join(8181..8315,1..15)
                     /locus_tag="L6451_RS29125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788727.1"
                     /translation="MEREKCSLKEAKNAYNRKWRAANRDKVKLYNDRYWEKKAKEMEEQ
                     ACQK"
                     /name="hypothetical protein"
     gene            56..274
                     /locus_tag="L6451_RS29130"
                     /old_locus_tag="BCM0105_p201"
     CDS             56..274
                     /locus_tag="L6451_RS29130"
                     /old_locus_tag="BCM0105_p201"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796811.1"
                     /translation="MKHLEKVGIELLGRRDELLEQWEKEGLKEDSIFEDDLKFVEELMN
                     RNDELMFDVKVELITTMDEIHHQKMGY"
                     /name="hypothetical protein"
     gene            279..530
                     /locus_tag="L6451_RS29135"
                     /old_locus_tag="BCM0105_p202"
     CDS             279..530
                     /locus_tag="L6451_RS29135"
                     /old_locus_tag="BCM0105_p202"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788731.1"
                     /translation="MLGKYDGLLEKERLLKRIDELSFALEASGDPMLEAMVKNSGNTDI
                     AVAKELITKGSELGMNEHVFKKALLAKVNELREEVTKL"
                     /name="hypothetical protein"
     gene            527..757
                     /locus_tag="L6451_RS29140"
                     /old_locus_tag="BCM0105_p203"
     CDS             527..757
                     /locus_tag="L6451_RS29140"
                     /old_locus_tag="BCM0105_p203"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796813.1"
                     /translation="MKKDKQKDLPFNHKVVLSVLPLGRSKAITTKEIMARSGIKDKRAV
                     SAILSELVTVYKQPIATTSKKNLRAFLLYRM"
                     /name="hypothetical protein"
     gene            909..1616
                     /locus_tag="L6451_RS29145"
                     /old_locus_tag="BCM0105_p205"
     CDS             909..1616
                     /locus_tag="L6451_RS29145"
                     /old_locus_tag="BCM0105_p205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788735.1"
                     /translation="MQTISKFFNVDNESKLNELTIAKGELNSKESELQSKLSRVERALE
                     LAQTDLMIDDSTANKKVVAKYEEAQEKFQSELIATQEELNKVSDEIAAIVKEELHAELV
                     DAAKQDVMLIELPEKCRKAKKVINRDLYRATSFEDEGRFSRKYLHVSATVIDDARTFAD
                     IHRFADKPNLTRIGYQRGELIRWGGDNNLFKLNDEYETIYEEEAQKIKDKTDKEYEQFM
                     KAFNKYFAVSDSH"
                     /name="hypothetical protein"
     gene            1670..2095
                     /locus_tag="L6451_RS29150"
                     /old_locus_tag="BCM0105_p206"
     CDS             1670..2095
                     /locus_tag="L6451_RS29150"
                     /old_locus_tag="BCM0105_p206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788712.1"
                     /translation="MSKDTLKNIFHVYCFYTVRLQGDTEPRMRRNKLNYDNPIQNYYES
                     FIDCLRGFCEVRSDVSLYSFYQFVVDAVNSLNKQERKLIYERYLYKDHYKSDRQHYLAM
                     DITPQNYKKQMDPVRRKLIKNLGIEDLQLNIPDWMKR"
                     /name="hypothetical protein"
     gene            2226..2474
                     /locus_tag="L6451_RS29155"
                     /old_locus_tag="BCM0105_p207"
     CDS             2226..2474
                     /locus_tag="L6451_RS29155"
                     /old_locus_tag="BCM0105_p207"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236788716.1"
                     /translation="MKSLYRILELDKDEVSSSEKLILLTIAIYSDNKFIPFSITELENY
                     TNLSRATVTRLVKQLEEKGFVEVQRNGTATNNYKVTL"
                     /name="helix-turn-helix domain-containing protein"
     gene            2551..2727
                     /locus_tag="L6451_RS29160"
                     /old_locus_tag="BCM0105_p208"
     CDS             2551..2727
                     /locus_tag="L6451_RS29160"
                     /old_locus_tag="BCM0105_p208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176543923.1"
                     /translation="MLPQKEQLGHLAEKTELVKDLAWIAHDLLTEEDYTKENAAEALIK
                     VINRELSYVSKVR"
                     /name="hypothetical protein"
     gene            2769..2975
                     /locus_tag="L6451_RS29165"
                     /old_locus_tag="BCM0105_p209"
     CDS             2769..2975
                     /locus_tag="L6451_RS29165"
                     /old_locus_tag="BCM0105_p209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788718.1"
                     /translation="MANTVAKDKFEIVEKFVNACMLKGVSEDKSFKLVEELCTLIQQKR
                     HDEWFGLVDEALKEAGHEEGVTL"
                     /name="hypothetical protein"
     gene            2972..3103
                     /locus_tag="L6451_RS29475"
                     /old_locus_tag="BCM0105_p210"
     CDS             2972..3103
                     /locus_tag="L6451_RS29475"
                     /old_locus_tag="BCM0105_p210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272880692.1"
                     /translation="MSEVEMVLHEFEELLHKIISAAQHGDEQAMNHLDIVKAWLKDN"
                     /name="hypothetical protein"
     gene            3180..3419
                     /locus_tag="L6451_RS29170"
                     /old_locus_tag="BCM0105_p211"
     CDS             3180..3419
                     /locus_tag="L6451_RS29170"
                     /old_locus_tag="BCM0105_p211"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788720.1"
                     /translation="MAEEHRYHVTFYFSNGKEFDGKVTSKYNKEDYLEGLEDLFLKEKT
                     LLIDHLGMLIQTKYITHVKVIEVGSKIGADKKDT"
                     /name="hypothetical protein"
     gene            3480..4016
                     /locus_tag="L6451_RS29175"
                     /old_locus_tag="BCM0105_p212"
     CDS             3480..4016
                     /locus_tag="L6451_RS29175"
                     /old_locus_tag="BCM0105_p212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_236788722.1"
                     /translation="MTDKTDSSQSIYISKDVAMMLKIQESTLRKYCIMLEEHGYKFHKN
                     EHGHRGFLDNDVITLRKLIEIKSHPDMTLKQACNAIMTWVKEKDMSEVDTNVITENEQH
                     DERYSELKEMIQQQNELLKKMAKKLDDQQRYIDEKLEKRDQQLMGAIREMQEEKRVLLE
                     TASASQEKKWYKFWK"
                     /name="DUF3967 domain-containing protein"
     gene            4639..5238
                     /locus_tag="L6451_RS29180"
                     /old_locus_tag="BCM0105_p213"
     CDS             4639..5238
                     /locus_tag="L6451_RS29180"
                     /old_locus_tag="BCM0105_p213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562260.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_236788723.1"
                     /translation="MNKRFAYIRVSDKTQNENRQLDEMLQQEIEERDIFIDKASGKDFN
                     RPKYQALKQMLRAGDILYIKSIDRFGRNKDDILKEWNEITKEIGADIVVLDMPLLDTRQ
                     YKDVLGSFVSDLVLQILSFFAEEERRKIKERQREGIDAAQRRGAKFGRPAATFPPGFEE
                     VYKRWKDGEIKAIEAMEELELKRTTFYKLVNSFENK"
                     /name="recombinase family protein"
     gene            5994..6191
                     /locus_tag="L6451_RS29185"
                     /old_locus_tag="BCM0105_p214"
     CDS             5994..6191
                     /locus_tag="L6451_RS29185"
                     /old_locus_tag="BCM0105_p214"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788725.1"
                     /translation="MIGKDMITLDNPKKEYFQQYAQNNKEQINANARRRRQQQKEKVEK
                     YDRLVAALRSRGISVEELLK"
                     /name="hypothetical protein"
     gene            6613..7713
                     /locus_tag="L6451_RS29190"
                     /old_locus_tag="BCM0105_p215"
     CDS             6613..7713
                     /locus_tag="L6451_RS29190"
                     /old_locus_tag="BCM0105_p215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205124.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_098492605.1"
                     /translation="MNHEEFINSTEIDDKFLKQIIDNREFNDVIVSYYERLHKESRDDG
                     MKKKQENVKGCNDFWIIDKYHEHRLKEFKRTNLCKDKFCNNCKKVKQASRMGKFIPLIQ
                     PYAENMYQLTLTIPNVKGDELKDTLSKMQKAFARLIEYFKGKKKINGIDFEVLQYEGAI
                     RSLEITFNGNEYHPHYHALIVLKVNKFHDCMIRKHKNVYSIDFTGKREARLFSDIEVLI
                     QKVWYLIINGKRITETAIQSLKKGYSCQLDKFSENDFVEIFKYMTKATTEDGKTLSYKQ
                     FKTLYYALFKVRQIQGYGIFYNLKDDEEINFDEVNEAYAEFIEELQTKEKPVTVSEAPE
                     QVRKESDYTVFSRKTFQKYWKQVMSE"
                     /name="protein rep"
ORIGIN
        1 gcatgtcaga agtaagtatg gaaactgtta ttaaggaaag cagcagagtg aactgttgaa
       61 gcatttagaa aaagtaggta ttgaattact ggggcgtaga gatgaattgt tagagcagtg
      121 ggaaaaagaa ggacttaaag aagacagcat ttttgaagat gatcttaaat tcgttgaaga
      181 attaatgaac cgaaatgacg aattaatgtt tgatgtaaaa gtggaattaa ttacaactat
      241 ggatgagatt catcatcaga aaatggggta ttgataggat gttaggaaag tatgatggat
      301 tattagaaaa agaacggtta cttaaacgaa ttgatgaact gtcattcgcg ttagaagctt
      361 caggtgaccc tatgttggaa gcgatggtta aaaatagtgg caatacagac atcgcggttg
      421 caaaagagtt gattacaaaa gggagtgaat taggtatgaa tgagcacgta tttaaaaaag
      481 cgttgctagc aaaagtaaat gaacttcgtg aagaggtaac gaaactatga aaaaagacaa
      541 acaaaaagac ctccctttta atcacaaagt agtattgagc gtactacctt taggaagatc
      601 taaagcaata acaactaaag aaattatggc acgtagtgga ataaaagaca agcgtgcagt
      661 atctgctatt ttaagtgaat tagtgactgt atataaacaa cctattgcta caacgtctaa
      721 aaagaattta agggcgtttt tattgtacag gatgtaatag attatcagat tggtaaagaa
      781 ggccttgtga gcagaaaaag gcatatagat aagcgtgatt cagcttatac agaagcgtgc
      841 ttggaaatat tcaaaggtac agtgacatta gatgagtcaa aaaacaaagt ggaaggaagt
      901 gttcgtgcat gcaaacaatc agcaagttct tcaatgtaga taacgagagt aagttaaacg
      961 aattaacaat agcaaaaggg gaattgaatt ccaaagaatc agaattacaa agtaagctat
     1021 ctcgagtaga aagagctctg gaactggcac aaactgactt aatgattgat gatagtacag
     1081 ctaataaaaa ggttgttgca aaatatgaag aagctcaaga gaagttccaa tcagagttaa
     1141 tagctactca agaagaactt aacaaggttt ctgatgagat tgcagctatc gttaaggaag
     1201 aattgcatgc tgaattagta gatgccgcta aacaggacgt tatgctaatc gaactacctg
     1261 aaaagtgcag aaaagcgaaa aaagtaatta acagggatct ttatagggcg acgtcctttg
     1321 aagatgaagg tcgatttagt agaaagtatt tgcatgttag tgctacagtg atcgacgacg
     1381 ctcgtacttt cgccgatata catcgttttg ctgacaaacc aaatctaaca cgaatagggt
     1441 atcaacgcgg tgaattgata cgttggggtg gcgataataa cctgttcaaa ttgaatgatg
     1501 aatatgaaac gatatatgaa gaagaagcac agaaaatcaa agacaaaacg gataaagagt
     1561 atgagcagtt catgaaggct ttcaataagt atttcgctgt gtcggattct cattaatcaa
     1621 aatacataag tcgcctaagg gcggcttttt tttattagga gggattttca tgagtaaaga
     1681 tacattaaaa aacatctttc acgtgtattg tttttataca gtacgtttgc aaggcgatac
     1741 agaaccacgt atgagacgaa acaagctaaa ttatgataat cctatacaaa actattatga
     1801 aagttttata gactgtctga gaggattctg tgaggttagg agcgacgttt cattgtattc
     1861 gttctatcaa tttgttgtag atgctgttaa ttcgttaaat aagcaggaaa gaaagctgat
     1921 ctatgagcgg tatttatata aagatcacta caaatcagat aggcaacatt accttgcgat
     1981 ggatataaca ccacagaatt ataaaaaaca gatggatccg gtaagaagga agttaattaa
     2041 gaacctcgga atagaagatt tacagttgaa tattccagat tggatgaaac gataacgggt
     2101 taaattacat gtatggttat attattttta aattttattt tgattctatt gcattttact
     2161 atactcattg ttatactaga taatgtaaac aacatgttga tttataggag gttactagtt
     2221 taattatgaa atcactatat cgtattcttg aattggacaa agatgaagtt tcttcttctg
     2281 aaaaactaat cttgctaacg atagcgatct attcagacaa caagtttatc ccattctcta
     2341 ttacagaatt ggagaattac acgaatttga gtagagctac ggttacaagg ctcgtaaaac
     2401 agttagaaga aaagggattc gtcgaggttc aaaggaatgg aacagcaaca aacaattaca
     2461 aagtaacact gtaaaaacaa agaaagagca ggagattgcg gctcctactc tttcaaaaca
     2521 cattatccct tatggaaagg atgtattagt atgttaccac aaaaagaaca gttaggacat
     2581 cttgctgaga aaactgaatt agttaaggat ttagcttgga ttgcacatga tttacttacc
     2641 gaggaagatt acaccaaaga gaatgcagcg gaagcgttaa ttaaggtcat taatagggaa
     2701 ttatcatacg tttccaaagt gcgttaaacg ggattatatg taaataaaac aattcggagg
     2761 taatattcat ggcaaataca gttgctaaag ataaatttga gatcgtagaa aagtttgtga
     2821 atgcttgtat gttaaaaggt gtttcggaag ataaatcatt caaattagta gaggaattat
     2881 gtacacttat tcaacaaaaa cgtcatgatg aatggtttgg attagtagat gaagcactta
     2941 aagaagcggg acatgaagaa ggtgtaacgt tatgagtgaa gtagaaatgg ttttacacga
     3001 attcgaagaa ctattacata aaatcatatc agcagcgcaa catggcgatg aacaagcgat
     3061 gaatcattta gatatagtaa aagcatggtt aaaggacaat taaggcgtgg tgatccacgt
     3121 ctttttttta tactgtcttg gggaatccta ctgatatatt ttgaaagtag gtggactaaa
     3181 tggctgaaga acatagatac catgtaactt tttatttttc taacggaaaa gaatttgatg
     3241 gtaaggtaac aagcaaatac aacaaagaag attatttaga agggttagaa gatctattcc
     3301 ttaaagaaaa gacattactg attgatcatc taggaatgtt aatacaaacc aaatatatta
     3361 cacatgtcaa agttatcgaa gttggatcta aaataggagc agacaaaaaa gatacgtgaa
     3421 ttattaagga aaaggatata tcattgttat aacgaatgtt ttggtagagg tgaagcgtta
     3481 tgacagataa aacagacagc tctcaatcca tatatataag caaagacgta gcgatgatgc
     3541 ttaaaattca agaatcgact ttacgtaaat attgtatcat gcttgaggaa cacggctaca
     3601 aatttcataa gaatgagcac ggccatagag gattccttga taacgatgtt ataacgttaa
     3661 gaaaattaat cgagattaaa tcacatcctg atatgacatt aaaacaggcc tgtaacgcaa
     3721 ttatgacgtg ggttaaagag aaagatatgt cagaggttga tacgaatgtt ataacggaaa
     3781 atgagcaaca tgacgagcga tatagtgagc ttaaagaaat gattcagcag cagaatgaat
     3841 tattaaagaa aatggcgaag aaattagatg atcagcagag gtatattgat gaaaagttag
     3901 aaaaacgaga tcagcagtta atgggagcta tccgagaaat gcaagaagaa aaacgagttt
     3961 tgttagaaac ggcttccgct agtcaagaaa agaaatggta caagttttgg aagtaggatt
     4021 ccctaactat ggggagtcct ttttatttta aagtgagttt ggatcactta gaggtagatt
     4081 aaacagaaag ccctgcaggt aacatcctca gaaacgcaca agaaaagtgg tgcgtataac
     4141 tgggcactcg cttttttctc gtgaataaga tctcattgaa tggagtaatt ttgtatacta
     4201 ctccgttgga atatagaacg ttttacggag cgttccaata tcgttacctt tgcaaaacaa
     4261 acacgaatac cacgttactt taatccgcac acaaggcacc ctttttatgc agtactcatt
     4321 gtagtattgc acccctttta atgttgtact gcaaaacaaa cacaactcaa ctaataaaga
     4381 acaaagcaca tataccggta attgaatggt aacgggtgtt aaggaatgat aaggagtatt
     4441 agtgcaaaat gaatgtagta gtattttagt aatttgcaaa acaaacgaac tcaattaatg
     4501 tattcgtaaa agtacacatt tacgaataag aaaaagaata agaaaaagat attgttttgt
     4561 gttcgtataa gttgtatgat tagtttacaa acattcgtaa attgaaaaac acttttacga
     4621 acaaagggga agaggaaaat gaacaaacgt ttcgcttata tccgtgtatc agataaaact
     4681 caaaacgaaa atagacagtt ggacgagatg ttgcagcagg agattgaaga aagagatatc
     4741 ttcattgaca aggcaagcgg taaagatttt aatcgtccta aataccaggc tttaaaacag
     4801 atgctacgtg ccggagatat actgtatatc aaatctattg atcgttttgg tagaaataaa
     4861 gatgatattt taaaagagtg gaatgaaata acgaaggaga tcggtgcgga tatcgttgta
     4921 ttggacatgc ctttattaga tacaagacaa tataaagatg tattaggctc gtttgtaagt
     4981 gatttggtac tacagatact atcattcttt gcagaagaag aaagacggaa aatcaaagag
     5041 agacaacgtg agggcattga tgccgcacaa agacgaggtg cgaagttcgg aagaccggct
     5101 gctacattcc cgccaggatt cgaagaagtg tacaagcgtt ggaaagatgg agaaattaaa
     5161 gcaatagaag ctatggaaga attagagtta aaacggacta ctttctataa actagtaaat
     5221 tcattcgaaa ataagtaaaa aaagtaataa taatattaaa ctttttacaa aattaagtcg
     5281 aaaaagcaaa gttaaaaaaa gacaaacata ccgataacaa gcattgatat tacacataat
     5341 tttatgcctt tcatgtcgaa aagtaaaagt taaaaaagta ataaaaaagc aaagtataaa
     5401 aagtatactt ttaagtttgc ttgtttctat gaaaatccgt tggtataata gttttaacgt
     5461 gtttgagtgt ttcaaacgaa aagaaaaaag ccgagttggg gaactcgact taagtctttc
     5521 taaaagtatg gggatacttt tagattagat tttgtgtctc gacaacactt tcgactaatt
     5581 agcactacca atgcttatta gtcattgatg attttacgaa tcatcttaac gattattttt
     5641 ttatggattg acacctattt tatcgacctt tgacaatcaa ataattaggt accatttata
     5701 tctaagattg aattaacaac cttatttaac gtatgaaaaa gtactgtagg tcaagttgta
     5761 aactataaaa agtttcttct taggaaacct taagagaaaa aaatatagat acaatttgta
     5821 tcgctttaat acgaaaaaaa gttctcgttc agcgaacttc ctatgtgaca agtttacaat
     5881 gtatttttcg ttttgacaat actttaagta aactcatagt ttttacatac attgaaaggt
     5941 acctctacta tttgaggtgc cttttttatt tatttttgaa aggatgatag aaaatgatag
     6001 ggaaagatat gatcacatta gacaacccaa agaaagagta ttttcagcaa tatgcacaaa
     6061 ataataaaga acaaattaac gccaatgcaa gaagacgaag acaacaacaa aaagaaaagg
     6121 tagagaagta tgatagattg gttgcggcgt tacgatctag aggaattagt gtagaagaat
     6181 tattgaaata agatggacaa gctgtattaa ggccggacta agacaccagg agaagatttg
     6241 tatacggtct aggttattcg tggggcttat gctacagctg tcattgtcta gcggtatcgc
     6301 agtgcgtaag cacgagaata aggagcagac atacaccaca agccccgaac actttcatgc
     6361 tctccaaaac aacatgtttt ggggatacct agtgtaacgt ggccgagtgc aacggaaggt
     6421 tttaatgttt cgtttaaaaa tgagtttctt ctattacttg atatattaga aataagtcgg
     6481 ctatttctct agttttctag taatgacaaa ttgtcgataa ccatagtcat atcaagggtt
     6541 taagctattc gacttattgc taataaggct taaaaagcct tccaaaaatt ccactagaaa
     6601 tgaggtgaga aattgaatca tgaggaattt ataaattcaa ctgaaataga tgataaattt
     6661 ttaaagcaaa tcattgataa tagggaattt aatgacgtta tagtctcgta ttatgaacga
     6721 ctacataaag aatcacgcga tgatggaatg aagaagaagc aggaaaacgt taaaggttgc
     6781 aatgattttt ggattataga caaatatcat gaacatagat taaaagagtt taaacgaact
     6841 aatctttgca aggacaagtt ttgtaacaac tgtaaaaagg ttaaacaagc ttcacgcatg
     6901 ggtaaattca taccacttat tcagccttac gcggaaaata tgtatcaatt aacgctaact
     6961 attcctaatg ttaaaggcga tgaattgaaa gatacactaa gcaagatgca aaaagctttt
     7021 gctcgtttaa ttgagtattt caaaggcaaa aagaaaataa acggaattga ttttgaagtt
     7081 ttacaatacg aaggcgctat acgatcttta gaaataacat ttaacggtaa tgagtatcat
     7141 ccacattatc atgctcttat tgttttaaag gttaataaat tccatgattg catgataaga
     7201 aaacataaaa atgtttattc aatagacttc actggaaaac gtgaggcgcg tttatttagt
     7261 gatatagagg ttttgataca gaaggtttgg tacttgatta taaacggtaa acggataaca
     7321 gaaacagcga tacagtcact taaaaaaggt tattcatgtc agttggacaa gtttagcgaa
     7381 aacgattttg tagagatatt taagtacatg acaaaagcaa caacggaaga cggtaaaaca
     7441 ttgagttata agcaattcaa aacgttgtat tatgcgttat ttaaagttag acaaatacaa
     7501 gggtatggca tcttttataa tttgaaggat gatgaagaaa ttaattttga tgaagttaat
     7561 gaagcgtatg cggaattcat agaggaatta cagacaaaag aaaaacctgt tacggtatca
     7621 gaagcaccag aacaggtcag aaaagaaagt gactatacag tgtttagtcg aaaaacgttc
     7681 caaaaatatt ggaagcaagt catgagcgaa tagccttact cattccggca agtttgataa
     7741 aaggctatct ttaccaccat taatcaaata acggagggtt ttctaatatc attatacata
     7801 attttaattc gtatgatatt aggaaattcc ttctatatcg ttaattagta gagactatga
     7861 attatttcgt agtctttttt tattggaaag gaatgattgt tgttatattt agaggtttat
     7921 aacaggaaaa aggcgcctga tctcgtttga atcagaacga aatcaagcgc ctttgggcga
     7981 taattaacca agaacaagtt aattaaataa atctaaaagt ttttggaagt acttatatta
     8041 tatcgtatat ggagttcccg gacagtaatt ttagcctaaa tttgcgtgaa gcaaatcatt
     8101 aatcatacca cgtaaactat atattcgtaa ataggtaaat gttaaaataa gagtaagaag
     8161 aatgaaaaga aggtggaaaa gtggaaagag aaaagtgttc tttgaaagaa gctaaaaacg
     8221 cttataaccg aaaatggaga gcagcgaatc gcgataaagt gaagctgtat aacgatagat
     8281 actgggaaaa gaaagctaaa gaaatggagg aacaa
//
