LOCUS       NZ_AP022906            10097 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0079 plasmid pMRY14-0079_3, complete
            sequence.
ACCESSION   NZ_AP022906
VERSION     NZ_AP022906.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203327
            Sequence Read Archive: DRR206367, DRR206391
            Assembly: GCF_021654975.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10097)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 335x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021654975.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:06:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,906
            CDSs (total)                      :: 5,754
            Genes (coding)                    :: 5,476
            CDSs (with protein)               :: 5,476
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 278
            CDSs (without protein)            :: 278
            Pseudo Genes (ambiguous residues) :: 0 of 278
            Pseudo Genes (frameshifted)       :: 156 of 278
            Pseudo Genes (incomplete)         :: 134 of 278
            Pseudo Genes (internal stop)      :: 47 of 278
            Pseudo Genes (multiple problems)  :: 53 of 278
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022906.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0079"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0079_3"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            82..405
                     /locus_tag="L6495_RS29300"
                     /old_locus_tag="BCM0079_p301"
     CDS             82..405
                     /locus_tag="L6495_RS29300"
                     /old_locus_tag="BCM0079_p301"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796016.1"
                     /translation="MMMNLLSLQQEANSVIQDLNGKDISSMSLIQKANYSNRIVKVRMS
                     LEAYRNTPGVNEFINALDHLRFKLAEVQQQETRQQANKRRAEIIQNGIKETPYPTMPMF
                     LNN"
                     /name="hypothetical protein"
     gene            417..755
                     /locus_tag="L6495_RS29305"
                     /old_locus_tag="BCM0079_p302"
     CDS             417..755
                     /locus_tag="L6495_RS29305"
                     /old_locus_tag="BCM0079_p302"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="WP_236796014.1"
                     /translation="MSDNVNFPLRIPRELRDKFKKVSQKTGTSMNSSICQLIAEHIYKM
                     EQLGLKDIEEPNVIQVTPVKEETKAFIKKAIKEADEAKELAETKTKKRLRKSPKKKIGG
                     ETDGSKKD"
                     /name="Arc family DNA-binding protein"
     gene            736..975
                     /locus_tag="L6495_RS29310"
                     /old_locus_tag="BCM0079_p303"
     CDS             736..975
                     /locus_tag="L6495_RS29310"
                     /old_locus_tag="BCM0079_p303"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796012.1"
                     /translation="MEVKKIKIKPKKIVRNEENHKLEFDKVRLSIEIPISIRDNFKEAV
                     EKNGKTMKEVLEAYMQRYSVKELLTDLKEETIDN"
                     /name="hypothetical protein"
     gene            1459..1659
                     /locus_tag="L6495_RS29315"
                     /old_locus_tag="BCM0079_p304"
     CDS             1459..1659
                     /locus_tag="L6495_RS29315"
                     /old_locus_tag="BCM0079_p304"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796011.1"
                     /translation="MDIEAKKEAFVTFLVEKVLFQTFAERTTSLEEKEVYEKFIKLYDQ
                     ELNRLNFELTIASVNRKRANL"
                     /name="hypothetical protein"
     gene            complement(1683..1829)
                     /locus_tag="L6495_RS29320"
                     /old_locus_tag="BCM0079_p305"
     CDS             complement(1683..1829)
                     /locus_tag="L6495_RS29320"
                     /old_locus_tag="BCM0079_p305"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796009.1"
                     /translation="MKKTKKQAKKQPKKQPKKQMKTAMKIVGITKDVVNFVGNLIRIYT
                     FFF"
                     /name="hypothetical protein"
     gene            2698..2850
                     /locus_tag="L6495_RS29325"
                     /old_locus_tag="BCM0079_p306"
     CDS             2698..2850
                     /locus_tag="L6495_RS29325"
                     /old_locus_tag="BCM0079_p306"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796023.1"
                     /translation="MPKVVSSKEKMQPKTFKLHPRTIEQIKELAEKKQMTQAEVIDKAL
                     KLLAR"
                     /name="hypothetical protein"
     gene            2943..3665
                     /locus_tag="L6495_RS29330"
                     /old_locus_tag="BCM0079_p307"
     CDS             2943..3665
                     /locus_tag="L6495_RS29330"
                     /old_locus_tag="BCM0079_p307"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236796021.1"
                     /translation="MKKAFAENYINPVVERFSELDTPNGGFYTSIPQAVQYCWFLDDKE
                     LSVLLALSTYADENGRCKVYQRTLARKVKIKSTTTLRTKLKSLVKKRFILQVNTDGESS
                     IYQIKDYSTNPYILLSEAIEKVEQVAKKKYGVSVSKIAETMDELIASKIDYMAMITKIE
                     ESFNGYDVALKSVQGYVNEALGLEMDISLQTKKPRRERGFKMSKVIKPKQPKRNREFII
                     SEGESEAPVKREGLRLRL"
                     /name="helix-turn-helix domain-containing protein"
     gene            3868..4713
                     /locus_tag="L6495_RS29335"
                     /old_locus_tag="BCM0079_p308"
     CDS             3868..4713
                     /locus_tag="L6495_RS29335"
                     /old_locus_tag="BCM0079_p308"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_236796018.1"
                     /translation="METDISIDRLTLVGYDTGMIEGYLKYSEYISFVGFSKNYGFRHSY
                     QGIKGELLEIGEKGKVRLDFNPNTANMEQVRDVVSRLKYIYLTRLDIAIDYFGVDLSEV
                     EWTSPKRRKQNLWLDPEGQQETRYIGAPSSERRYRIYNKKLERLEKKEELDPRAVNGQW
                     RVEVQQRFKESNNILDPIDYFTGDLFDIRPYRKVVDLSHIEKPMERIIIAGLMARPSEL
                     KNLSKNARGKYKKMIQEAREKSGVAINPEPIEVYTKEKDLLAEQLTSLFAHCAKSVAQV
                     "
                     /name="replication initiation factor domain-containing
                     protein"
     gene            5131..5454
                     /locus_tag="L6495_RS29340"
                     /old_locus_tag="BCM0079_p309"
     CDS             5131..5454
                     /locus_tag="L6495_RS29340"
                     /old_locus_tag="BCM0079_p309"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796016.1"
                     /translation="MMMNLLSLQQEANSVIQDLNGKDISSMSLIQKANYSNRIVKVRMS
                     LEAYRNTPGVNEFINALDHLRFKLAEVQQQETRQQANKRRAEIIQNGIKETPYPTMPMF
                     LNN"
                     /name="hypothetical protein"
     gene            5466..5804
                     /locus_tag="L6495_RS29345"
                     /old_locus_tag="BCM0079_p310"
     CDS             5466..5804
                     /locus_tag="L6495_RS29345"
                     /old_locus_tag="BCM0079_p310"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="WP_236796014.1"
                     /translation="MSDNVNFPLRIPRELRDKFKKVSQKTGTSMNSSICQLIAEHIYKM
                     EQLGLKDIEEPNVIQVTPVKEETKAFIKKAIKEADEAKELAETKTKKRLRKSPKKKIGG
                     ETDGSKKD"
                     /name="Arc family DNA-binding protein"
     gene            5785..6024
                     /locus_tag="L6495_RS29350"
                     /old_locus_tag="BCM0079_p311"
     CDS             5785..6024
                     /locus_tag="L6495_RS29350"
                     /old_locus_tag="BCM0079_p311"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796012.1"
                     /translation="MEVKKIKIKPKKIVRNEENHKLEFDKVRLSIEIPISIRDNFKEAV
                     EKNGKTMKEVLEAYMQRYSVKELLTDLKEETIDN"
                     /name="hypothetical protein"
     gene            6508..6708
                     /locus_tag="L6495_RS29355"
                     /old_locus_tag="BCM0079_p312"
     CDS             6508..6708
                     /locus_tag="L6495_RS29355"
                     /old_locus_tag="BCM0079_p312"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796011.1"
                     /translation="MDIEAKKEAFVTFLVEKVLFQTFAERTTSLEEKEVYEKFIKLYDQ
                     ELNRLNFELTIASVNRKRANL"
                     /name="hypothetical protein"
     gene            complement(6732..6878)
                     /locus_tag="L6495_RS29360"
                     /old_locus_tag="BCM0079_p313"
     CDS             complement(6732..6878)
                     /locus_tag="L6495_RS29360"
                     /old_locus_tag="BCM0079_p313"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796009.1"
                     /translation="MKKTKKQAKKQPKKQPKKQMKTAMKIVGITKDVVNFVGNLIRIYT
                     FFF"
                     /name="hypothetical protein"
     gene            7747..7899
                     /locus_tag="L6495_RS29365"
                     /old_locus_tag="BCM0079_p314"
     CDS             7747..7899
                     /locus_tag="L6495_RS29365"
                     /old_locus_tag="BCM0079_p314"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236796023.1"
                     /translation="MPKVVSSKEKMQPKTFKLHPRTIEQIKELAEKKQMTQAEVIDKAL
                     KLLAR"
                     /name="hypothetical protein"
     gene            7992..8606
                     /locus_tag="L6495_RS29370"
                     /old_locus_tag="BCM0079_p315"
     CDS             7992..8606
                     /locus_tag="L6495_RS29370"
                     /old_locus_tag="BCM0079_p315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236799402.1"
                     /translation="MKKAFAENYINPVVERFSELDTPNGGFYTSIPQAVQYCWFLDDKE
                     LSVLLALSTYADENGRCKVYQRTLARKVKIKSTTTLRTKLKSLVKKRFILQVNTDGESS
                     IYQIKDYSTNPYILLSEAIEKVEQVAKKKYGVSVSKIAETMDELIASKIDYMAMITKIE
                     ESFNGYDVALKSVQGYVNEALGLEMDISLQTKNLDGNEVLR"
                     /name="helix-turn-helix domain-containing protein"
     gene            8916..9761
                     /locus_tag="L6495_RS29375"
                     /old_locus_tag="BCM0079_p316"
     CDS             8916..9761
                     /locus_tag="L6495_RS29375"
                     /old_locus_tag="BCM0079_p316"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_236796018.1"
                     /translation="METDISIDRLTLVGYDTGMIEGYLKYSEYISFVGFSKNYGFRHSY
                     QGIKGELLEIGEKGKVRLDFNPNTANMEQVRDVVSRLKYIYLTRLDIAIDYFGVDLSEV
                     EWTSPKRRKQNLWLDPEGQQETRYIGAPSSERRYRIYNKKLERLEKKEELDPRAVNGQW
                     RVEVQQRFKESNNILDPIDYFTGDLFDIRPYRKVVDLSHIEKPMERIIIAGLMARPSEL
                     KNLSKNARGKYKKMIQEAREKSGVAINPEPIEVYTKEKDLLAEQLTSLFAHCAKSVAQV
                     "
                     /name="replication initiation factor domain-containing
                     protein"
ORIGIN
        1 atgtttttat aaatgactat catattgata atcaatttaa tagtcgtatt gattatcaca
       61 acagaaaaga gggaaaatat tatgatgatg aatttgttaa gtttacaaca ggaagctaac
      121 agtgtgattc aagatttaaa cggtaaggat atttcttcaa tgagtttgat ccagaaggct
      181 aattattcta atcgaatagt gaaagttaga atgagccttg aggcatatag aaatactccg
      241 ggagtaaatg aatttataaa tgcattggac cacttaagat ttaagttagc tgaagtacaa
      301 caacaggaaa cacgacaaca ggctaataaa agaagagctg aaataattca aaatggaata
      361 aaagaaacac cgtacccaac gatgccaatg tttctaaata attaggagga taggatatga
      421 gcgataatgt taattttcca ttgagaatac ctcgtgaatt gcgggataag tttaaaaagg
      481 tttcacagaa gactgggacg agtatgaact catcaatttg ccaactaata gcggaacaca
      541 tttataaaat ggagcaatta ggattgaagg atatagaaga acctaatgta attcaagtta
      601 cgccggttaa agaagaaaca aaggcgttta taaagaaggc tataaaagaa gctgatgaag
      661 ctaaggaatt agctgaaact aagacgaaaa aaagattaag aaagtcgccc aagaagaaaa
      721 taggcggtga aacagatgga agtaaaaaag attaagatca aacctaaaaa aatagtgcgt
      781 aatgaagaaa atcataaatt agaatttgat aaggtaagat taagcattga aatccctatt
      841 tctattagag ataactttaa agaagcggta gaaaagaacg ggaagacgat gaaggaagta
      901 ttagaagcat atatgcaaag gtatagtgtt aaagagctat taacggattt aaaagaagaa
      961 acaatagata attagaatct gaatacctcg tttaaacctt gaatacatgt acatttttaa
     1021 ggcttaaatt ttatagttgg taaaagtaga aaaaacacga cctatcaata aaaatatcgc
     1081 taaatttacg ctaaacaact ataagtattc ttcctaagat agtggtgaac caggaggcat
     1141 tcatgtgagg gcatagtctt tgcggtttac ggtatgtcgg tttttaggga agaaatggcg
     1201 aggtttaacg aggattggcg aagaattaag ggttaaatta attggttttc atcgagtatt
     1261 agcgaagatt agggaagacc actaaggaat caaagcgtag cggttcattt acaatgagaa
     1321 tgtccgtggt agcctgttat ggcttgccaa tccttgtttg agcgttagcg agttgaagga
     1381 gtggggcatt ggcttatgac ccacggactg gaggctcgtt gtcaatgatt gaattccgct
     1441 tattggagga agtaagttat ggatatagaa gctaagaaag aagcatttgt gaccttcctt
     1501 gtagagaaag ttctatttca aacatttgca gaaaggacta cttctttaga agaaaaagag
     1561 gtgtatgaaa agtttattaa gctatatgat caagaattaa atagactaaa tttcgaattg
     1621 acaatagcat ctgtaaatag aaaaagagcc aacctataat gttatagatt agctcttttt
     1681 gtttagaaga agaatgtata aattcggatt agatttccaa caaaattgac cacgtctttt
     1741 gttattccga ctattttcat tgctgtcttc atttgtttct taggttgttt cttaggttgt
     1801 ttttttgctt gttttttagt tttcttcata aattaattcc ccctatttag tagttgttcg
     1861 tcttgctatc tattacttgt gacgtttttt gagaaatcct tatttttttt attctttttc
     1921 aaaaaaattt cttttataaa cataggcgca gatactaagg ttttttggtt tgctatatgt
     1981 ataaaacttg agtaaaaata tcaatttgga ttgtcaatgg aataacacct cctagttttc
     2041 tattcgtctt ttcatctatg ttttgtgaca ctttttgaga gatttgtatt aagagttttt
     2101 ttcttcaaag agataggcac attatattgt tcgtgttggt gtgattcttt tcttagttaa
     2161 attttagagc ttttcttcat agtatttatc cttctcttta tccttaattt atccgtgatt
     2221 tatccgtctt tttatcgaga ttatcctcta tagaaaagtg tatcttggat aaagattgaa
     2281 aaaggtatct aatttagtag cccaaatagt agctcaaaag tgccattact ctttctatac
     2341 aaaacaataa cgatttttca tttgaaaata gtctcccaaa aagtttgggt tttgttcgga
     2401 tgattaagaa ctatgtattc tagttcttgc tctgattttt tataactata gggatgttcg
     2461 acacgtcgaa aaacccgctt ctctaacagc gggttagcgt cctctttcga gggattcacc
     2521 atatcaaaat gttttttgtt ttaggttttc tcctttcttt tttgatcaaa aaaatgtcct
     2581 tattagtttg ttctattttt ttatgtgttt tttgactgtt tttgaagtgg acaaatagaa
     2641 tcgttgatgt taagctgttt ttagggagta cctatcattg tgttgaggag tgatattatg
     2701 ccgaaagtag ttagttctaa ggaaaagatg caacccaaaa catttaaatt gcatcctagg
     2761 acgattgaac aaataaagga attagctgaa aagaagcaaa tgacgcaagc tgaagtgatt
     2821 gataaggcgt taaagttatt agcacgataa aaaaatatat atcccatcgc aaataacgta
     2881 gcatactaac atagcaaaca agataacaag gagggaaaat taaaaggaaa aggtgatctg
     2941 taatgaaaaa ggcatttgca gagaattata ttaatcctgt tgtggaacgt tttagtgaat
     3001 tagatactcc aaatggcggt ttctatactt ccattccaca agcagttcaa tactgttggt
     3061 ttttagatga taaagagtta agtgtattat tagcactatc gacctatgca gacgaaaatg
     3121 gacgttgcaa ggtatatcaa cgaacgttag ctaggaaagt taagattaaa agtacaacaa
     3181 cattacgaac taaattgaaa agtttagtga agaagcgttt tatattgcag gttaatacag
     3241 atggagaatc tagtatttat cagattaaag attattctac caatccttat attcttctta
     3301 gtgaggctat tgaaaaagtc gaacaggtag ctaaaaagaa atatggtgtt tcagtatcta
     3361 aaatagctga aacgatggat gaattaattg ctagtaagat agattatatg gctatgataa
     3421 ctaagataga ggaaagtttt aatggttatg atgtagcttt aaaatctgta caaggttatg
     3481 taaatgaggc tttaggatta gaaatggata tttctttaca aacgaaaaaa cctcgacggg
     3541 aacgaggttt taagatgagt aaagttatta aacctaagca acctaaacgt aacagagaat
     3601 ttattatttc agagggtgaa tctgaagctc ctgtgaaacg ggaaggtctt agattacgac
     3661 tttgaataag gtaattatat attaccttat aggacttgaa tacctagtca aaaattgatt
     3721 accccttaca caatttttgt gtacctctta atcaaaattt gattactact aaatataaaa
     3781 tactaaaaag aaattaatat atatatctat caaagaaatt actgaagcat actaagatag
     3841 caaataaaac ggaaatgagt gtttaggatg gaaacagata ttagtataga ccgcctaact
     3901 ctcgttggat acgatactgg aatgatagaa gggtatctta aatactcgga atatatttca
     3961 ttcgtggggt tttctaagaa ttatggtttt cgtcattcat accaagggat aaaaggagaa
     4021 ttgcttgaga taggcgagaa agggaaggta cgtttagatt ttaaccctaa taccgcaaat
     4081 atggagcagg taagagacgt cgtttctcga ctgaaataca tctatttaac gcgattagat
     4141 atagcgatag attattttgg tgtagattta tctgaggttg agtggacaag ccctaaacga
     4201 agaaaacaaa acttatggtt agacccagaa gggcaacaag aaacgagata cataggtgcg
     4261 ccatcaagtg agagaaggta tcgtatttat aacaagaagt tagaacggtt agaaaagaaa
     4321 gaagagttag accctagagc agttaatgga cagtggcgtg ttgaggtaca acaacgtttt
     4381 aaagagtcca ataacatatt agaccctatt gattatttta caggtgatct attcgatatt
     4441 agaccatatc gaaaggtggt tgatttatct catatagaaa agcctatgga acgaattata
     4501 atagcgggat taatggcaag acctagtgaa cttaagaatc ttagtaagaa cgctagaggt
     4561 aagtataaga agatgataca agaggctagg gaaaaaagcg gtgtagcgat aaatcctgaa
     4621 ccaatagaag tttatacaaa agaaaaagac ttgttagctg agcaactaac aagcctgttc
     4681 gcccactgtg ctaagagcgt agcacaagtg taaactttaa aacttataac aagtaaatat
     4741 ctaatgttag tatagcaaaa gaagagaggg aattcacctc tctttttctt tgttttaagg
     4801 ggattcgtta agaatatatg tatttccttt aagtttattg cgttagctcg tgctttagga
     4861 ttaatagttg tctatttagg ctattgtttg aaaaacaccc cacccaccgc ttattaggtt
     4921 ctgtatctga aaatgcgctc ctacaccctc tcgattttgt taatttttat ataggtaagg
     4981 tgacaattta aaaataaatt ttaaattttt taagtaaccc agtatacaga tttttggtta
     5041 agagttacga tgtttttata aatgactatc atattgataa tcaatttaat agtcgtattg
     5101 attatcacaa cagaaaagag ggaaaatatt atgatgatga atttgttaag tttacaacag
     5161 gaagctaaca gtgtgattca agatttaaac ggtaaggata tttcttcaat gagtttgatc
     5221 cagaaggcta attattctaa tcgaatagtg aaagttagaa tgagccttga ggcatataga
     5281 aatactccgg gagtaaatga atttataaat gcattggacc acttaagatt taagttagct
     5341 gaagtacaac aacaggaaac acgacaacag gctaataaaa gaagagctga aataattcaa
     5401 aatggaataa aagaaacacc gtacccaacg atgccaatgt ttctaaataa ttaggaggat
     5461 aggatatgag cgataatgtt aattttccat tgagaatacc tcgtgaattg cgggataagt
     5521 ttaaaaaggt ttcacagaag actgggacga gtatgaactc atcaatttgc caactaatag
     5581 cggaacacat ttataaaatg gagcaattag gattgaagga tatagaagaa cctaatgtaa
     5641 ttcaagttac gccggttaaa gaagaaacaa aggcgtttat aaagaaggct ataaaagaag
     5701 ctgatgaagc taaggaatta gctgaaacta agacgaaaaa aagattaaga aagtcgccca
     5761 agaagaaaat aggcggtgaa acagatggaa gtaaaaaaga ttaagatcaa acctaaaaaa
     5821 atagtgcgta atgaagaaaa tcataaatta gaatttgata aggtaagatt aagcattgaa
     5881 atccctattt ctattagaga taactttaaa gaagcggtag aaaagaacgg gaagacgatg
     5941 aaggaagtat tagaagcata tatgcaaagg tatagtgtta aagagctatt aacggattta
     6001 aaagaagaaa caatagataa ttagaatctg aatacctcgt ttaaaccttg aatacatgta
     6061 catttttaag gcttaaattt tatagttggt aaaagtagaa aaaacacgac ctatcaataa
     6121 aaatatcgct aaatttacgc taaacaacta taagtattct tcctaagata gtggtgaacc
     6181 aggaggcatt catgtgaggg catagtcttt gcggtttacg gtatgtcggt ttttagggaa
     6241 gaaatggcga ggtttaacga ggattggcga agaattaagg gttaaattaa ttggttttca
     6301 tcgagtatta gcgaagatta gggaagacca ctaaggaatc aaagcgtagc ggttcattta
     6361 caatgagaat gtccgtggta gcctgttatg gcttgccaat ccttgtttga gcgttagcga
     6421 gttgaaggag tggggcattg gcttatgacc cacggactgg aggctcgttg tcaatgattg
     6481 aattccgctt attggaggaa gtaagttatg gatatagaag ctaagaaaga agcatttgtg
     6541 accttccttg tagagaaagt tctatttcaa acatttgcag aaaggactac ttctttagaa
     6601 gaaaaagagg tgtatgaaaa gtttattaag ctatatgatc aagaattaaa tagactaaat
     6661 ttcgaattga caatagcatc tgtaaataga aaaagagcca acctataatg ttatagatta
     6721 gctctttttg tttagaagaa gaatgtataa attcggatta gatttccaac aaaattgacc
     6781 acgtcttttg ttattccgac tattttcatt gctgtcttca tttgtttctt aggttgtttc
     6841 ttaggttgtt tttttgcttg ttttttagtt ttcttcataa attaattccc cctatttagt
     6901 agttgttcgt cttgctatct attacttgtg acgttttttg agaaatcctt atttttttta
     6961 ttctttttca aaaaaatttc ttttataaac ataggcgcag atactaaggt tttttggttt
     7021 gctatatgta taaaacttga gtaaaaatat caatttggat tgtcaatgga ataacacctc
     7081 ctagttttct attcgtcttt tcatctatgt tttgtgacac tttttgagag atttgtatta
     7141 agagtttttt tcttcaaaga gataggcaca ttatattgtt cgtgttggtg tgattctttt
     7201 cttagttaaa ttttagagct tttcttcata gtatttatcc ttctctttat ccttaattta
     7261 tccgtgattt atccgtcttt ttatcgagat tatcctctat agaaaagtgt atcttggata
     7321 aagattgaaa aaggtatcta atttagtagc ccaaatagta gctcaaaagt gccattactc
     7381 tttctataca aaacaataac gatttttcat ttgaaaatag tctcccaaaa agtttgggtt
     7441 ttgttcggat gattaagaac tatgtattct agttcttgct ctgatttttt ataactatag
     7501 ggatgttcga cacgtcgaaa aacccgcttc tctaacagcg ggttagcgtc ctctttcgag
     7561 ggattcacca tatcaaaatg ttttttgttt taggttttct cctttctttt ttgatcaaaa
     7621 aaatgtcctt attagtttgt tctatttttt tatgtgtttt ttgactgttt ttgaagtgga
     7681 caaatagaat cgttgatgtt aagctgtttt tagggagtac ctatcattgt gttgaggagt
     7741 gatattatgc cgaaagtagt tagttctaag gaaaagatgc aacccaaaac atttaaattg
     7801 catcctagga cgattgaaca aataaaggaa ttagctgaaa agaagcaaat gacgcaagct
     7861 gaagtgattg ataaggcgtt aaagttatta gcacgataaa aaaatatata tcccatcgca
     7921 aataacgtag catactaaca tagcaaacaa gataacaagg agggaaaatt aaaaggaaaa
     7981 ggtgatctgt aatgaaaaag gcatttgcag agaattatat taatcctgtt gtggaacgtt
     8041 ttagtgaatt agatactcca aatggcggtt tctatacttc cattccacaa gcagttcaat
     8101 actgttggtt tttagatgat aaagagttaa gtgtattatt agcactatcg acctatgcag
     8161 acgaaaatgg acgttgcaag gtatatcaac gaacgttagc taggaaagtt aagattaaaa
     8221 gtacaacaac attacgaact aaattgaaaa gtttagtgaa gaagcgtttt atattgcagg
     8281 ttaatacaga tggagaatct agtatttatc agattaaaga ttattctacc aatccttata
     8341 ttcttcttag tgaggctatt gaaaaagtcg aacaggtagc taaaaagaaa tatggtgttt
     8401 cagtatctaa aatagctgaa acgatggatg aattaattgc tagtaagata gattatatgg
     8461 ctatgataac taagatagag gaaagtttta atggttatga tgtagcttta aaatctgtac
     8521 aaggttatgt aaatgaggct ttaggattag aaatggatat ttctttacaa acgaaaaacc
     8581 tcgacgggaa cgaggtttta agatgagtaa agttattaaa cctaagcaac ctaaacgtaa
     8641 cagagaattt attatttcag agggtgaatc tgaagctcct gtgaaacggg aaggtcttag
     8701 attacgactt tgaataaggt aattatatat taccttatag gacttgaata cctagtcaaa
     8761 aattgattac cccttacaca atttttgtgt acctcttaat caaaatttga ttactactaa
     8821 atataaaata ctaaaaagaa attaatatat atatctatca aagaaattac tgaagcatac
     8881 taagatagca aataaaacgg aaatgagtgt ttaggatgga aacagatatt agtatagacc
     8941 gcctaactct cgttggatac gatactggaa tgatagaagg gtatcttaaa tactcggaat
     9001 atatttcatt cgtggggttt tctaagaatt atggttttcg tcattcatac caagggataa
     9061 aaggagaatt gcttgagata ggcgagaaag ggaaggtacg tttagatttt aaccctaata
     9121 ccgcaaatat ggagcaggta agagacgtcg tttctcgact gaaatacatc tatttaacgc
     9181 gattagatat agcgatagat tattttggtg tagatttatc tgaggttgag tggacaagcc
     9241 ctaaacgaag aaaacaaaac ttatggttag acccagaagg gcaacaagaa acgagataca
     9301 taggtgcgcc atcaagtgag agaaggtatc gtatttataa caagaagtta gaacggttag
     9361 aaaagaaaga agagttagac cctagagcag ttaatggaca gtggcgtgtt gaggtacaac
     9421 aacgttttaa agagtccaat aacatattag accctattga ttattttaca ggtgatctat
     9481 tcgatattag accatatcga aaggtggttg atttatctca tatagaaaag cctatggaac
     9541 gaattataat agcgggatta atggcaagac ctagtgaact taagaatctt agtaagaacg
     9601 ctagaggtaa gtataagaag atgatacaag aggctaggga aaaaagcggt gtagcgataa
     9661 atcctgaacc aatagaagtt tatacaaaag aaaaagactt gttagctgag caactaacaa
     9721 gcctgttcgc ccactgtgct aagagcgtag cacaagtgta aactttaaaa cttataacaa
     9781 gtaaatatct aatgttagta tagcaaaaga agagagggaa ttcacctctc tttttctttg
     9841 ttttaagggg attcgttaag aatatatgta tttcctttaa gtttattgcg ttagctcgtg
     9901 ctttaggatt aatagttgtc tatttaggct attgtttgaa aaacacccca cccaccgctt
     9961 attaggttct gtatctgaaa atgcgctcct acaccctctc gattttgtta atttttatat
    10021 aggtaaggtg acaatttaaa aataaatttt aaatttttta agtaacccag tatacagatt
    10081 tttggttaag agttacg
//
