LOCUS       NZ_AP022905             8319 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0079 plasmid pMRY14-0079_2, complete
            sequence.
ACCESSION   NZ_AP022905
VERSION     NZ_AP022905.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203327
            Sequence Read Archive: DRR206367, DRR206391
            Assembly: GCF_021654975.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 335x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021654975.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:06:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,906
            CDSs (total)                      :: 5,754
            Genes (coding)                    :: 5,476
            CDSs (with protein)               :: 5,476
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 278
            CDSs (without protein)            :: 278
            Pseudo Genes (ambiguous residues) :: 0 of 278
            Pseudo Genes (frameshifted)       :: 156 of 278
            Pseudo Genes (incomplete)         :: 134 of 278
            Pseudo Genes (internal stop)      :: 47 of 278
            Pseudo Genes (multiple problems)  :: 53 of 278
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022905.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0079"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0079_2"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            join(7529..8319,1..310)
                     /locus_tag="L6495_RS29235"
     CDS             join(7529..8319,1..310)
                     /locus_tag="L6495_RS29235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205124.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_098492605.1"
                     /translation="MNHEEFINSTEIDDKFLKQIIDNREFNDVIVSYYERLHKESRDDG
                     MKKKQENVKGCNDFWIIDKYHEHRLKEFKRTNLCKDKFCNNCKKVKQASRMGKFIPLIQ
                     PYAENMYQLTLTIPNVKGDELKDTLSKMQKAFARLIEYFKGKKKINGIDFEVLQYEGAI
                     RSLEITFNGNEYHPHYHALIVLKVNKFHDCMIRKHKNVYSIDFTGKREARLFSDIEVLI
                     QKVWYLIINGKRITETAIQSLKKGYSCQLDKFSENDFVEIFKYMTKATTEDGKTLSYKQ
                     FKTLYYALFKVRQIQGYGIFYNLKDDEEINFDEVNEAYAEFIEELQTKEKPVTVSEAPE
                     QVRKESDYTVFSRKTFQKYWKQVMSE"
                     /name="protein rep"
     gene            799..927
                     /locus_tag="L6495_RS29545"
                     /old_locus_tag="BCM0079_p201"
     CDS             799..927
                     /locus_tag="L6495_RS29545"
                     /old_locus_tag="BCM0079_p201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272880717.1"
                     /translation="MKEAKNAYNRKWRAANRDKVKLYNDRYWEKKAKEMEEQACQK"
                     /name="hypothetical protein"
     gene            915..1187
                     /locus_tag="L6495_RS29240"
                     /old_locus_tag="BCM0079_p202"
     CDS             915..1187
                     /locus_tag="L6495_RS29240"
                     /old_locus_tag="BCM0079_p202"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788729.1"
                     /translation="MSEVSMETVIKGKQQSELLKHLEKVGIELLGRRDELLEQWEKEGL
                     KEDSIFEDDLKFVEELMNRNDELMFDVKVELITTMDEIHHQKMGY"
                     /name="hypothetical protein"
     gene            1192..1443
                     /locus_tag="L6495_RS29245"
                     /old_locus_tag="BCM0079_p203"
     CDS             1192..1443
                     /locus_tag="L6495_RS29245"
                     /old_locus_tag="BCM0079_p203"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788731.1"
                     /translation="MLGKYDGLLEKERLLKRIDELSFALEASGDPMLEAMVKNSGNTDI
                     AVAKELITKGSELGMNEHVFKKALLAKVNELREEVTKL"
                     /name="hypothetical protein"
     gene            1440..1853
                     /locus_tag="L6495_RS29250"
                     /old_locus_tag="BCM0079_p204"
     CDS             1440..1853
                     /locus_tag="L6495_RS29250"
                     /old_locus_tag="BCM0079_p204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788733.1"
                     /translation="MKKDKQKDLPFNHKVVLSVLPLGRSKAITTKEIMARSGIKDKRAV
                     SAILSELVTVYKQPIATTSKKEFKGVFIVQDVIDYQIGKEGLVSRKRHIDKRDSAYTEA
                     CLEIFKGTVTLDESKNKVEGSVRACKQSASSSM"
                     /name="hypothetical protein"
     gene            1823..2530
                     /locus_tag="L6495_RS29255"
                     /old_locus_tag="BCM0079_p205"
     CDS             1823..2530
                     /locus_tag="L6495_RS29255"
                     /old_locus_tag="BCM0079_p205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788735.1"
                     /translation="MQTISKFFNVDNESKLNELTIAKGELNSKESELQSKLSRVERALE
                     LAQTDLMIDDSTANKKVVAKYEEAQEKFQSELIATQEELNKVSDEIAAIVKEELHAELV
                     DAAKQDVMLIELPEKCRKAKKVINRDLYRATSFEDEGRFSRKYLHVSATVIDDARTFAD
                     IHRFADKPNLTRIGYQRGELIRWGGDNNLFKLNDEYETIYEEEAQKIKDKTDKEYEQFM
                     KAFNKYFAVSDSH"
                     /name="hypothetical protein"
     gene            2584..3009
                     /locus_tag="L6495_RS29260"
                     /old_locus_tag="BCM0079_p206"
     CDS             2584..3009
                     /locus_tag="L6495_RS29260"
                     /old_locus_tag="BCM0079_p206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788712.1"
                     /translation="MSKDTLKNIFHVYCFYTVRLQGDTEPRMRRNKLNYDNPIQNYYES
                     FIDCLRGFCEVRSDVSLYSFYQFVVDAVNSLNKQERKLIYERYLYKDHYKSDRQHYLAM
                     DITPQNYKKQMDPVRRKLIKNLGIEDLQLNIPDWMKR"
                     /name="hypothetical protein"
     gene            3140..3388
                     /locus_tag="L6495_RS29265"
                     /old_locus_tag="BCM0079_p207"
     CDS             3140..3388
                     /locus_tag="L6495_RS29265"
                     /old_locus_tag="BCM0079_p207"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236788716.1"
                     /translation="MKSLYRILELDKDEVSSSEKLILLTIAIYSDNKFIPFSITELENY
                     TNLSRATVTRLVKQLEEKGFVEVQRNGTATNNYKVTL"
                     /name="helix-turn-helix domain-containing protein"
     gene            3465..3641
                     /locus_tag="L6495_RS29270"
                     /old_locus_tag="BCM0079_p208"
     CDS             3465..3641
                     /locus_tag="L6495_RS29270"
                     /old_locus_tag="BCM0079_p208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176543923.1"
                     /translation="MLPQKEQLGHLAEKTELVKDLAWIAHDLLTEEDYTKENAAEALIK
                     VINRELSYVSKVR"
                     /name="hypothetical protein"
     gene            3683..3889
                     /locus_tag="L6495_RS29275"
                     /old_locus_tag="BCM0079_p209"
     CDS             3683..3889
                     /locus_tag="L6495_RS29275"
                     /old_locus_tag="BCM0079_p209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788718.1"
                     /translation="MANTVAKDKFEIVEKFVNACMLKGVSEDKSFKLVEELCTLIQQKR
                     HDEWFGLVDEALKEAGHEEGVTL"
                     /name="hypothetical protein"
     gene            3886..4017
                     /locus_tag="L6495_RS29550"
                     /old_locus_tag="BCM0079_p210"
     CDS             3886..4017
                     /locus_tag="L6495_RS29550"
                     /old_locus_tag="BCM0079_p210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272880692.1"
                     /translation="MSEVEMVLHEFEELLHKIISAAQHGDEQAMNHLDIVKAWLKDN"
                     /name="hypothetical protein"
     gene            4094..4333
                     /locus_tag="L6495_RS29280"
                     /old_locus_tag="BCM0079_p211"
     CDS             4094..4333
                     /locus_tag="L6495_RS29280"
                     /old_locus_tag="BCM0079_p211"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788720.1"
                     /translation="MAEEHRYHVTFYFSNGKEFDGKVTSKYNKEDYLEGLEDLFLKEKT
                     LLIDHLGMLIQTKYITHVKVIEVGSKIGADKKDT"
                     /name="hypothetical protein"
     gene            4394..4930
                     /locus_tag="L6495_RS29285"
                     /old_locus_tag="BCM0079_p212"
     CDS             4394..4930
                     /locus_tag="L6495_RS29285"
                     /old_locus_tag="BCM0079_p212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_236788722.1"
                     /translation="MTDKTDSSQSIYISKDVAMMLKIQESTLRKYCIMLEEHGYKFHKN
                     EHGHRGFLDNDVITLRKLIEIKSHPDMTLKQACNAIMTWVKEKDMSEVDTNVITENEQH
                     DERYSELKEMIQQQNELLKKMAKKLDDQQRYIDEKLEKRDQQLMGAIREMQEEKRVLLE
                     TASASQEKKWYKFWK"
                     /name="DUF3967 domain-containing protein"
     gene            5554..6153
                     /locus_tag="L6495_RS29290"
                     /old_locus_tag="BCM0079_p213"
     CDS             5554..6153
                     /locus_tag="L6495_RS29290"
                     /old_locus_tag="BCM0079_p213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562260.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_236788723.1"
                     /translation="MNKRFAYIRVSDKTQNENRQLDEMLQQEIEERDIFIDKASGKDFN
                     RPKYQALKQMLRAGDILYIKSIDRFGRNKDDILKEWNEITKEIGADIVVLDMPLLDTRQ
                     YKDVLGSFVSDLVLQILSFFAEEERRKIKERQREGIDAAQRRGAKFGRPAATFPPGFEE
                     VYKRWKDGEIKAIEAMEELELKRTTFYKLVNSFENK"
                     /name="recombinase family protein"
     gene            6909..7106
                     /locus_tag="L6495_RS29295"
                     /old_locus_tag="BCM0079_p214"
     CDS             6909..7106
                     /locus_tag="L6495_RS29295"
                     /old_locus_tag="BCM0079_p214"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788725.1"
                     /translation="MIGKDMITLDNPKKEYFQQYAQNNKEQINANARRRRQQQKEKVEK
                     YDRLVAALRSRGISVEELLK"
                     /name="hypothetical protein"
ORIGIN
        1 gtacatgaca aaagcaacaa cggaagacgg taaaacattg agttataagc aattcaaaac
       61 gttgtattat gcgttattta aagttagaca aatacaaggg tatggcatct tttataattt
      121 gaaggatgat gaagaaatta attttgatga agttaatgaa gcgtatgcgg aattcataga
      181 ggaattacag acaaaagaaa aacctgttac ggtatcagaa gcaccagaac aggtcagaaa
      241 agaaagtgac tatacagtgt ttagtcgaaa aacgttccaa aaatattgga agcaagtcat
      301 gagcgaatag ccttactcat tccggcaagt ttgataaaag gctatcttta ccaccattaa
      361 tcaaataacg gagggttttc taatatcatt atacataatt ttaattcgta tgatattagg
      421 aaattccttc tatatcgtta attagtagag actatgaatt atttcgtagt ctttttttat
      481 tggaaaggaa tgattgttgt tatatttaga ggtttataac aggaaaaagg cgcctgatct
      541 cgtttgaatc agaacgaaat caagcgcctt tgggcgataa ttaaccaaga acaagttaat
      601 taaataaatc taaaagtttt tggaagtact tatattatat cgtatatgga gttcccggac
      661 agtaatttta gcctaaattt gcgtgaagca aatcattaat cataccacgt aaactatata
      721 ttcgtaaata ggtaaatgtt aaaataagag taagaagaat gaaaagaagg tggaaaagtg
      781 gaaagagaaa agtgttcttt gaaagaagct aaaaacgctt ataaccgaaa atggagagca
      841 gcgaatcgcg ataaagtgaa gctgtataac gatagatact gggaaaagaa agctaaagaa
      901 atggaggaac aagcatgtca gaagtaagta tggaaactgt tattaaagga aagcagcaga
      961 gtgaactgtt gaagcattta gaaaaagtag gtattgaatt actggggcgt agagatgaat
     1021 tgttagagca gtgggaaaaa gaaggactta aagaagacag catttttgaa gatgatctta
     1081 aattcgttga agaattaatg aaccgaaatg acgaattaat gtttgatgta aaagtggaat
     1141 taattacaac tatggatgag attcatcatc agaaaatggg gtattgatag gatgttagga
     1201 aagtatgatg gattattaga aaaagaacgg ttacttaaac gaattgatga actgtcattc
     1261 gcgttagaag cttcaggtga ccctatgttg gaagcgatgg ttaaaaatag tggcaataca
     1321 gacatcgcgg ttgcaaaaga gttgattaca aaagggagtg aattaggtat gaatgagcac
     1381 gtatttaaaa aagcgttgct agcaaaagta aatgaacttc gtgaagaggt aacgaaacta
     1441 tgaaaaaaga caaacaaaaa gacctccctt ttaatcacaa agtagtattg agcgtactac
     1501 ctttaggaag atctaaagca ataacaacta aagaaattat ggcacgtagt ggaataaaag
     1561 acaagcgtgc agtatctgct attttaagtg aattagtgac tgtatataaa caacctattg
     1621 ctacaacgtc taaaaaagaa tttaagggcg tttttattgt acaggatgta atagattatc
     1681 agattggtaa agaaggcctt gtgagcagaa aaaggcatat agataagcgt gattcagctt
     1741 atacagaagc gtgcttggaa atattcaaag gtacagtgac attagatgag tcaaaaaaca
     1801 aagtggaagg aagtgttcgt gcatgcaaac aatcagcaag ttcttcaatg tagataacga
     1861 gagtaagtta aacgaattaa caatagcaaa aggggaattg aattccaaag aatcagaatt
     1921 acaaagtaag ctatctcgag tagaaagagc tctggaactg gcacaaactg acttaatgat
     1981 tgatgatagt acagctaata aaaaggttgt tgcaaaatat gaagaagctc aagagaagtt
     2041 ccaatcagag ttaatagcta ctcaagaaga acttaacaag gtttctgatg agattgcagc
     2101 tatcgttaag gaagaattgc atgctgaatt agtagatgcc gctaaacagg acgttatgct
     2161 aatcgaacta cctgaaaagt gcagaaaagc gaaaaaagta attaacaggg atctttatag
     2221 ggcgacgtcc tttgaagatg aaggtcgatt tagtagaaag tatttgcatg ttagtgctac
     2281 agtgatcgac gacgctcgta ctttcgccga tatacatcgt tttgctgaca aaccaaatct
     2341 aacacgaata gggtatcaac gcggtgaatt gatacgttgg ggtggcgata ataacctgtt
     2401 caaattgaat gatgaatatg aaacgatata tgaagaagaa gcacagaaaa tcaaagacaa
     2461 aacggataaa gagtatgagc agttcatgaa ggctttcaat aagtatttcg ctgtgtcgga
     2521 ttctcattaa tcaaaataca taagtcgcct aagggcggct tttttttatt aggagggatt
     2581 ttcatgagta aagatacatt aaaaaacatc tttcacgtgt attgttttta tacagtacgt
     2641 ttgcaaggcg atacagaacc acgtatgaga cgaaacaagc taaattatga taatcctata
     2701 caaaactatt atgaaagttt tatagactgt ctgagaggat tctgtgaggt taggagcgac
     2761 gtttcattgt attcgttcta tcaatttgtt gtagatgctg ttaattcgtt aaataagcag
     2821 gaaagaaagc tgatctatga gcggtattta tataaagatc actacaaatc agataggcaa
     2881 cattaccttg cgatggatat aacaccacag aattataaaa aacagatgga tccggtaaga
     2941 aggaagttaa ttaagaacct cggaatagaa gatttacagt tgaatattcc agattggatg
     3001 aaacgataac gggttaaatt acatgtatgg ttatattatt tttaaatttt attttgattc
     3061 tattgcattt tactatactc attgttatac tagataatgt aaacaacatg ttgatttata
     3121 ggaggttact agtttaatta tgaaatcact atatcgtatt cttgaattgg acaaagatga
     3181 agtttcttct tctgaaaaac taatcttgct aacgatagcg atctattcag acaacaagtt
     3241 tatcccattc tctattacag aattggagaa ttacacgaat ttgagtagag ctacggttac
     3301 aaggctcgta aaacagttag aagaaaaggg attcgtcgag gttcaaagga atggaacagc
     3361 aacaaacaat tacaaagtaa cactgtaaaa acaaagaaag agcaggagat tgcggctcct
     3421 actctttcaa aacacattat cccttatgga aaggatgtat tagtatgtta ccacaaaaag
     3481 aacagttagg acatcttgct gagaaaactg aattagttaa ggatttagct tggattgcac
     3541 atgatttact taccgaggaa gattacacca aagagaatgc agcggaagcg ttaattaagg
     3601 tcattaatag ggaattatca tacgtttcca aagtgcgtta aacgggatta tatgtaaata
     3661 aaacaattcg gaggtaatat tcatggcaaa tacagttgct aaagataaat ttgagatcgt
     3721 agaaaagttt gtgaatgctt gtatgttaaa aggtgtttcg gaagataaat cattcaaatt
     3781 agtagaggaa ttatgtacac ttattcaaca aaaacgtcat gatgaatggt ttggattagt
     3841 agatgaagca cttaaagaag cgggacatga agaaggtgta acgttatgag tgaagtagaa
     3901 atggttttac acgaattcga agaactatta cataaaatca tatcagcagc gcaacatggc
     3961 gatgaacaag cgatgaatca tttagatata gtaaaagcat ggttaaagga caattaaggc
     4021 gtggtgatcc acgtcttttt tttatactgt cttggggaat cctactgata tattttgaaa
     4081 gtaggtggac taaatggctg aagaacatag ataccatgta actttttatt tttctaacgg
     4141 aaaagaattt gatggtaagg taacaagcaa atacaacaaa gaagattatt tagaagggtt
     4201 agaagatcta ttccttaaag aaaagacatt actgattgat catctaggaa tgttaataca
     4261 aaccaaatat attacacatg tcaaagttat cgaagttgga tctaaaatag gagcagacaa
     4321 aaaagatacg tgaattatta aggaaaagga tatatcattg ttataacgaa tgttttggta
     4381 gaggtgaagc gttatgacag ataaaacaga cagctctcaa tccatatata taagcaaaga
     4441 cgtagcgatg atgcttaaaa ttcaagaatc gactttacgt aaatattgta tcatgcttga
     4501 ggaacacggc tacaaatttc ataagaatga gcacggccat agaggattcc ttgataacga
     4561 tgttataacg ttaagaaaat taatcgagat taaatcacat cctgatatga cattaaaaca
     4621 ggcctgtaac gcaattatga cgtgggttaa agagaaagat atgtcagagg ttgatacgaa
     4681 tgttataacg gaaaatgagc aacatgacga gcgatatagt gagcttaaag aaatgattca
     4741 gcagcagaat gaattattaa agaaaatggc gaagaaatta gatgatcagc agaggtatat
     4801 tgatgaaaag ttagaaaaac gagatcagca gttaatggga gctatccgag aaatgcaaga
     4861 agaaaaacga gttttgttag aaacggcttc cgctagtcaa gaaaagaaat ggtacaagtt
     4921 ttggaagtag gattccctaa ctatggggag tcctttttta ttttaaagtg agtttggatc
     4981 acttagaggt agattaaaca gaaagccctg caggtaacat cctcagaaac gcacaagaaa
     5041 agtggtgcgt ataactgggc actcgctttt ttctcgtgaa taagatctca ttgaatggag
     5101 taattttgta tactactccg ttggaatata gaacgtttta cggagcgttc caatatcgtt
     5161 acctttgcaa aacaaacacg aataccacgt tactttaatc cgcacacaag gcaccctttt
     5221 tatgcagtac tcattgtagt attgcacccc ttttaatgtt gtactgcaaa acaaacacaa
     5281 ctcaactaat aaagaacaaa gcacatatac cggtaattga atggtaacgg gtgttaagga
     5341 atgataagga gtattagtgc aaaatgaatg tagtagtatt ttagtaattt gcaaaacaaa
     5401 cgaactcaat taatgtattc gtaaaagtac acatttacga ataagaaaaa gaataagaaa
     5461 aagatattgt tttgtgttcg tataagttgt atgattagtt tacaaacatt cgtaaattga
     5521 aaaacacttt tacgaacaaa ggggaagagg aaaatgaaca aacgtttcgc ttatatccgt
     5581 gtatcagata aaactcaaaa cgaaaataga cagttggacg agatgttgca gcaggagatt
     5641 gaagaaagag atatcttcat tgacaaggca agcggtaaag attttaatcg tcctaaatac
     5701 caggctttaa aacagatgct acgtgccgga gatatactgt atatcaaatc tattgatcgt
     5761 tttggtagaa ataaagatga tattttaaaa gagtggaatg aaataacgaa ggagatcggt
     5821 gcggatatcg ttgtattgga catgccttta ttagatacaa gacaatataa agatgtatta
     5881 ggctcgtttg taagtgattt ggtactacag atactatcat tctttgcaga agaagaaaga
     5941 cggaaaatca aagagagaca acgtgagggc attgatgccg cacaaagacg aggtgcgaag
     6001 ttcggaagac cggctgctac attcccgcca ggattcgaag aagtgtacaa gcgttggaaa
     6061 gatggagaaa ttaaagcaat agaagctatg gaagaattag agttaaaacg gactactttc
     6121 tataaactag taaattcatt cgaaaataag taaaaaaagt aataataata ttaaactttt
     6181 tacaaaatta agtcgaaaaa gcaaagttaa aaaaagacaa acataccgat aacaagcatt
     6241 gatattacac ataattttat gcctttcatg tcgaaaagta aaagttaaaa aagtaataaa
     6301 aaagcaaagt ataaaaagta tacttttaag tttgcttgtt tctatgaaaa tccgttggta
     6361 taatagtttt aacgtgtttg agtgtttcaa acgaaaagaa aaaagccgag ttggggaact
     6421 cgacttaagt ctttctaaaa gtatggggat acttttagat tagattttgt gtctcgacaa
     6481 cactttcgac taattagcac taccaatgct tattagtcat tgatgatttt acgaatcatc
     6541 ttaacgatta tttttttatg gattgacacc tattttatcg acctttgaca atcaaataat
     6601 taggtaccat ttatatctaa gattgaatta acaaccttat ttaacgtatg aaaaagtact
     6661 gtaggtcaag ttgtaaacta taaaaagttt cttcttagga aaccttaaga gaaaaaaata
     6721 tagatacaat ttgtatcgct ttaatacgaa aaaaagttct cgttcagcga acttcctatg
     6781 tgacaagttt acaatgtatt tttcgttttg acaatacttt aagtaaactc atagttttta
     6841 catacattga aaggtacctc tactatttga ggtgcctttt ttatttattt ttgaaaggat
     6901 gatagaaaat gatagggaaa gatatgatca cattagacaa cccaaagaaa gagtattttc
     6961 agcaatatgc acaaaataat aaagaacaaa ttaacgccaa tgcaagaaga cgaagacaac
     7021 aacaaaaaga aaaggtagag aagtatgata gattggttgc ggcgttacga tctagaggaa
     7081 ttagtgtaga agaattattg aaataagatg gacaagctgt attaaggccg gactaagaca
     7141 ccaggagaag atttgtatac ggtctaggtt attcgtgggg cttatgctac agctgtcatt
     7201 gtctagcggt atcgcagtgc gtaagcacga gaataaggag cagacataca ccacaagccc
     7261 cgaacacttt catgctctcc aaaacaacat gttttgggga tacctagtgt aacgtggccg
     7321 agtgcaacgg aaggttttaa tgtttcgttt aaaaaatgag tttcttctat tacttgatat
     7381 attagaaata agtcggctat ttctctagtt ttctagtaat gacaaattgt cgataaccat
     7441 agtcatatca agggtttaag ctattcgact tattgctaat aaggcttaaa aagccttcca
     7501 aaaattccac tagaaatgag gtgagaaatt gaatcatgag gaatttataa attcaactga
     7561 aatagatgat aaatttttaa agcaaatcat tgataatagg gaatttaatg acgttatagt
     7621 ctcgtattat gaacgactac ataaagaatc acgcgatgat ggaatgaaga agaagcagga
     7681 aaacgttaaa ggttgcaatg atttttggat tatagacaaa tatcatgaac atagattaaa
     7741 agagtttaaa cgaactaatc tttgcaagga caagttttgt aacaactgta aaaaggttaa
     7801 acaagcttca cgcatgggta aattcatacc acttattcag ccttacgcgg aaaatatgta
     7861 tcaattaacg ctaactattc ctaatgttaa aggcgatgaa ttgaaagata cactaagcaa
     7921 gatgcaaaaa gcttttgctc gtttaattga gtatttcaaa ggcaaaaaga aaataaacgg
     7981 aattgatttt gaagttttac aatacgaagg cgctatacga tctttagaaa taacatttaa
     8041 cggtaatgag tatcatccac attatcatgc tcttattgtt ttaaaggtta ataaattcca
     8101 tgattgcatg ataagaaaac ataaaaatgt ttattcaata gacttcactg gaaaacgtga
     8161 ggcgcgttta tttagtgata tagaggtttt gatacagaag gtttggtact tgattataaa
     8221 cggtaaacgg ataacagaaa cagcgataca gtcacttaaa aaaggttatt catgtcagtt
     8281 ggacaagttt agcgaaaacg attttgtaga gatatttaa
//
