LOCUS       NZ_AP022893             3164 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0074 plasmid pMRY14-0074_7, complete
            sequence.
ACCESSION   NZ_AP022893
VERSION     NZ_AP022893.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203325
            Sequence Read Archive: DRR206365, DRR206389
            Assembly: GCF_021654935.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 382x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021654935.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:54:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,677
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,280
            CDSs (with protein)               :: 5,280
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 148 of 244
            Pseudo Genes (incomplete)         :: 110 of 244
            Pseudo Genes (internal stop)      :: 40 of 244
            Pseudo Genes (multiple problems)  :: 42 of 244
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022893.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0074"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0074_7"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            complement(330..563)
                     /locus_tag="L6477_RS28240"
                     /old_locus_tag="BCM0074_p71"
     CDS             complement(330..563)
                     /locus_tag="L6477_RS28240"
                     /old_locus_tag="BCM0074_p71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="WP_046199669.1"
                     /translation="MTKLEELEKDFNQMNLDLKAIQHDMKSLEVRILVAEKDVLTINKQ
                     LDKISANTTWILRLIISGLLTGVLGVVAKNLL"
                     /name="hemolysin XhlA family protein"
     gene            1368..1868
                     /locus_tag="L6477_RS28245"
                     /old_locus_tag="BCM0074_p72"
     CDS             1368..1868
                     /locus_tag="L6477_RS28245"
                     /old_locus_tag="BCM0074_p72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182216.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_001182216.1"
                     /translation="MQPERPLKKVSKVTKNKIIDINTGEIQEEEEIINAYIDKEPDYVK
                     IYLKDIVALNDLPKGLDRVINVLLKVMSYDNMIILNSFIKKQMAQELGYKTVQVLNNNI
                     NKLVNAEILFRKGTGTYQVNARYFGRGHWHDIQEIRLQQVYSTKGRELKTEITYKNDEP
                     SQN"
                     /name="replication/maintenance protein RepL"
     gene            complement(1882..3126)
                     /gene="mobV"
                     /locus_tag="L6477_RS28250"
                     /old_locus_tag="BCM0074_p73"
     CDS             complement(1882..3126)
                     /gene="mobV"
                     /locus_tag="L6477_RS28250"
                     /old_locus_tag="BCM0074_p73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562244.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000951644.1"
                     /translation="MAHVQKYAKGNVQGLSIHWDRKTENHSNQDIDNERSHLNYDLCEK
                     EGDTLSRMNDRLSEVHCLNRKDVKVCADWAVTLPESLKDASEKEQREFFEKTYEFLANR
                     YGGEKNVLSANVHNDETRPHMHFAFMPVVWDEKKQQAKVSAKEVLTRKDLKTFHQDLDE
                     FLKKEIPQIYKEGILNDKTIGVDTVKDLKRYSEEIQKQKDVMDAEVKVKERELEGKIQS
                     LDKELESKKNEFLNLSEALPSKINIKVKGKEKKTEVVKTGFLKSETVTTETGNWIVSDS
                     EMRRMQKVLSDANFVKEDYERLQRTDLVKENKELHDRVDSLADGYVKAINENTDLYEKN
                     RELRKEISSLKAHVKDLKENVKVLYHNTKKVLGEHFKAFRGLVKNELDIKGVDNQFDCE
                     YKKEIKKQRGYNMER"
                     /name="mobV"
     Typing          complement(1886..3126)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggatagaaga aaaaccaaat ttggtctagc acgttatacc aaatttcatt tggacgtgct
       61 ctgcgaggct ctttggctcc gccaagccat gctttgcctg gcaaaaaaca aaccttgggc
      121 tttgcccaaa cccactgggg aaactcttcc ccagaccccc tcaatcaaac tccccaaccc
      181 ctcaatcctt gaggggctcc ctcgccggtt agcaggaaca aacgattgtt tgttcaatgc
      241 caagagggtt tgggtgtagt acggttggtc aagtcaaata ctcccttcgc tttgctaggt
      301 ccgttttgat tgaccaacac ccttttttcc tataacaggt ttttagctac tacaccaagt
      361 actcctgtta acaatccgct tatgatcaat cgtaaaatcc atgtagtatt tgcactaatt
      421 ttatctaatt gtttattaat agttaaaaca tccttttcag ctactaatat tcttacttct
      481 aaacttttca tatcatgttg tatagctttt aaatctagat tcatttgatt aaaatctttt
      541 tctagttcct ccaattttgt catataacat acttctcctt ttaaattaat tttaaatata
      601 ttatatgtat atggtaatta gagaataatg atataaaaag agaactcaaa tctattatta
      661 agatttaagt tctctttaaa gataagggaa ttatacgaaa gcgtaatata gcatatgctt
      721 atccttttta atatgcatct agcaaaaaca cagtttctat aaatgtgctg ttctaagttc
      781 attctctcct tgtttttggg tgtgagtgct ggctgggggt ttgggggcta gccccctgga
      841 tcttaacgta aatgaatatg gaataagcgg attgtttaga acgatagagc aaggcggttc
      901 ggcaggtaca attgctagtc caagtcgaac aatctaaatc ctcgtttttg gtctgttcgg
      961 cttggcgaca tgctcacacc tgccgaattt cactatgcaa tagcaaaaga gtggagattt
     1021 tttgaatggt tttctcaaaa aatactactc gactattgca tagtgaaaag cccttttaaa
     1081 ttttataaaa taatagaata gaagggagaa cataggttga accgttgata tgactggatt
     1141 tctaggaaaa tactataatt tgtgggatta taagttataa tttgtgggat tataaccgta
     1201 atttgtggag ttatataccg taatttgtgg agttataaat taaatttatt tttatatcat
     1261 ttataatttg tgggattata aatgtgaaat cctataaaaa tattgcactt ttaaaatacc
     1321 cgatttataa tttaattaaa ttataaatcg ggtaggaggt agatttgatg caaccggaaa
     1381 gacccttgaa aaaagtttct aaagttacaa aaaataaaat aatagatata aatactggtg
     1441 aaattcaaga agaagaagaa attataaatg cttatatcga caaagaacca gactacgtaa
     1501 aaatatattt aaaagatatt gtagccttaa atgatttacc taaaggttta gatagagtta
     1561 ttaacgtttt attaaaagta atgtcttatg acaatatgat cattctaaat agctttataa
     1621 aaaaacaaat ggcacaagaa ctaggatata aaacagtaca agttcttaat aacaacatca
     1681 acaaacttgt aaatgcagaa attcttttta gaaaaggtac tggtacatat caagtaaatg
     1741 ctcgttattt tggtcgtggc cattggcacg atatacaaga aatccgatta caacaagtct
     1801 attcaactaa aggaagagag cttaaaacag aaataacata caaaaacgac gaaccgtctc
     1861 agaattaatt ctgagacggt tttaacgttc catgttatat ccacgttgtt tcttaatttc
     1921 tttcttatat tcacaatcaa attgattatc tacacctttt atatccaatt cattcttaac
     1981 aagtcctcta aacgctttaa aatgctctcc aaggactttt ttcgtattgt ggtataaaac
     2041 ctttacattt tcctttaaat cttttacatg ggcttttaac gagcttattt ccttacgtaa
     2101 ttctctattc ttctcataca aatctgtatt ctcattaata gctttaacat aaccatcggc
     2161 taaactatca actctatcat gtaattcttt gttttcttta actaaatctg tacgctgcaa
     2221 acgctcataa tcttccttta cgaaatttgc atcgcttaga actttttgca tccttctcat
     2281 ttcactatca gaaacaatcc aattcccagt ttctgtagtt actgtttctg attttaaaaa
     2341 acctgttttt acaacctctg tttttttctc ttttcctttt actttgatat taatttttga
     2401 agggagtgct tcactcaaat tcagaaactc atttttttta ctttccaatt ctttatctaa
     2461 tgattgaatt ttcccctcta actctctttc tttgaccttt acttccgcat ccattacatc
     2521 tttttgtttc tgtatttctt cagaatacct tttcaaatct ttaaccgtat caacgccaat
     2581 cgttttatcg tttaaaatac cttctttgta gatttgtggt atttcttttt tcaaaaactc
     2641 atctaaatct tggtgaaatg tttttaaatc ttttcttgtt aaaacttctt tagctgaaac
     2701 ctttgcctgt tgctttttct cgtcccaaac aacaggcata aaagcaaaat gcatatgcgg
     2761 tcttgtttcg tcattatgta cattggctga caacacattt ttttcaccac cataacggtt
     2821 cgctaaaaat tcataggttt tctcaaaaaa ttcacgttgt tccttctcag aagcatcttt
     2881 caaactttcc ggtaatgtaa cagcccaatc tgcacagact tttacatctt ttcgattcaa
     2941 gcaatgcact tcacttaacc gatcattcat gcgtgaaagt gtatctcctt ctttttcaca
     3001 taaatcataa ttcaaatgtg accgttcatt atcaatgtct tgattcgaat gattttctgt
     3061 ttttctgtcc cagtggatgg acaatccttg aacatttcct tttgcatatt tttgaacgtg
     3121 agccatgagc aagacccccc cttttggtaa ctagtttaac atgg
//
