LOCUS       NZ_AP022892             4697 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0074 plasmid pMRY14-0074_6, complete
            sequence.
ACCESSION   NZ_AP022892
VERSION     NZ_AP022892.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203325
            Sequence Read Archive: DRR206365, DRR206389
            Assembly: GCF_021654935.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 382x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021654935.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:54:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,677
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,280
            CDSs (with protein)               :: 5,280
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 148 of 244
            Pseudo Genes (incomplete)         :: 110 of 244
            Pseudo Genes (internal stop)      :: 40 of 244
            Pseudo Genes (multiple problems)  :: 42 of 244
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022892.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0074"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0074_6"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            complement(324..701)
                     /gene="qacH"
                     /locus_tag="L6477_RS28215"
                     /old_locus_tag="BCM0074_p61"
     ARG             complement(328..701)
                     /name="qacH"
                     /gene="qacH"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacH"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="99.2"
                     /coverage="100.0"
     gene            complement(701..1264)
                     /locus_tag="L6477_RS28220"
                     /old_locus_tag="BCM0074_p62"
     CDS             complement(701..1264)
                     /locus_tag="L6477_RS28220"
                     /old_locus_tag="BCM0074_p62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002078386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_088027078.1"
                     /translation="MEVIMSISKHANKKKNILIAASEIVKEEGVVKLTLEAVAQRAGVS
                     KGGLLYHFPSKEALIKGMVEEWTNNYFECINTLVNNDDDNAIGKWNRAYLKSTFSDLEN
                     NNLNSALMAAMFINPDLLDEFRQRYDILHTKLITDGIDPVKITITRLSIDGLWFSEIFG
                     MAPLNEELKTQVFDELINMIQEDE"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            2292..2849
                     /locus_tag="L6477_RS28225"
                     /old_locus_tag="BCM0074_p64"
     CDS             2292..2849
                     /locus_tag="L6477_RS28225"
                     /old_locus_tag="BCM0074_p64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016125306.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_048558832.1"
                     /translation="MPAEVRKLDEQRKINVELTPEQLQMLQRLIDLEQQENKDKEQFEE
                     KKKNHNFIQLYRDNMPELRWLMANHNFASSLLFFILEHMDNRNALACSYAVFEDYFGKS
                     RSTVYRAVKLLEENGFLNVLKMGTSNVYVVNEDLAWTDGNDKKKFAKYDGKILVSKKEN
                     KDFEYKSQFDKFKALRERENLK"
                     /name="replication/maintenance protein RepL"
     gene            complement(2863..3060)
                     /locus_tag="L6477_RS28230"
                     /old_locus_tag="BCM0074_p65"
     CDS             complement(2863..3060)
                     /locus_tag="L6477_RS28230"
                     /old_locus_tag="BCM0074_p65"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048558833.1"
                     /translation="MKVKQLVEKLEKLDQEGELMLFMRDRKRPHLGSGTLIPIKDIGVF
                     TSVDQDSNEGKYALEIKKGE"
                     /name="hypothetical protein"
     gene            complement(3060..4406)
                     /gene="mobV"
                     /locus_tag="L6477_RS28235"
                     /old_locus_tag="BCM0074_p66"
     CDS             complement(3060..4406)
                     /gene="mobV"
                     /locus_tag="L6477_RS28235"
                     /old_locus_tag="BCM0074_p66"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_053082646.1"
                     /translation="MSYSIIRMQKMKSNGIKGIQFHNQRERESETNPDINKADSHLNYD
                     LINGQRQIDYNEKISQVIEDNVTSGKKIRKDAVKLAEFLITSDKEFFDKISPKEQKRYF
                     ETAHEFLADRYGEKNLIYATVHYDEKTPHMHVGFVPVTEDGRLSAKDFFGQKKQLVSLQ
                     DDFNQYLKQNDFNLERGVSSSRKHVETAKYKAQTFQSMEKEAQEKYERTMGHIQEIDDK
                     SKSIANIEAKKVLGLVGMKEQDYKSLVDYATNGVAYQLQAENLQKELEKTQKEVTQLKA
                     DMQIGQDKIRYYYKDIEENLDSLAEKKALQKVKQTDIVKNYGDLIEKYNGLVNKYNDQL
                     KEKKELKQQVDTLKIENRSYQNENRELKLENGKLKGKLVQISKEFSVFKERVGKVLHAQ
                     LDRVKTFLRMNDVDRSTIKFLDDRQDKLVQDSLEKMEKPQRQKGMEMER"
                     /name="mobV"
     ARG             complement(325..701)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="61.68"
                     /coverage="116.82"
     Typing          2293..2849
                     /name="rep_cluster_1925"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.818"
                     /coverage="100.0"
                     /evalue="1.87e-127"
ORIGIN
        1 tgacagacgc catacgggcc tctctcgcct ttgtaagagg ttgcccctag taaaaaccct
       61 taaaaagcaa aaaagacgct tctaggggta gcgtcaggga aatgcggatt tacaacggta
      121 agcatttgat acaagtctta tgagacagtc agaagtcatt taaaacgcta atttcattat
      181 gtctcaataa actctactct attggaattg tgaagtgata cacttaaact aacgtacgca
      241 ggtttgttga ataaggactt caaaaaatac ccttaattaa gctaaaaagt ccttctcttt
      301 attaagaagg acttttttat gtgttattcc attgtctcat tcattgtaat agtttcgctt
      361 gcttcatgac cagattctcc atgtcctggt ccaaacaaat tcaatattac aactcctaca
      421 acaatcagtg ttattcctgc aatgctagcc atgttaagtt tttccttcca aactaaaact
      481 gaaattactg tagtcaaaac aagtcctaaa cctgcccata atgcatatgc agtgtttaag
      541 gggattgttt ttagcgacaa ggataaaaag aaaaaacaac caacgaaagc tattattacc
      601 cctattgtag gatagagttt tgaaaagcct tcagatgctt ttaataggga agagccaata
      661 atctccccca ctattgcaag tgctaaatat agatatgaca ttattcgtcc tcctgaatca
      721 tgtttattag ttcatcaaaa acttgtgttt ttagttcttc atttaatggt gccattccaa
      781 atatttctga aaaccaaaga ccatctatgg ataatcttgt aattgttatt ttgacaggat
      841 caattccatc agttattaac tttgtatgca aaatatcgta tctctgccgg aattcatcta
      901 ataaatctgg attaataaac atagcagcca ttagtgcgga atttagatta ttgttttcta
      961 aatcggaaaa tgtagattta agataagccc tattccattt tcctattgcg ttatcatcgt
     1021 cgttattaac taacgtatta atgcattcga aataattatt tgtccattct tccaccattc
     1081 ctttaattaa tgcttccttg ctaggaaaat ggtataataa tccaccttta ctcacgccag
     1141 ctctttgggc gactgcctct aatgtgagtt tgacaactcc ttcttctttt acaatttcag
     1201 atgcagcaat caatatattt tttttcttat tagcatgctt tgaaatagac ataataacct
     1261 ccataactta gatacatata atataccttc tggacggttt ttttgtcaat cgaattaaag
     1321 ctcttattta atatcactct ttcacataaa aataatccaa taaattaatc taaagattgt
     1381 gaagaaatgt acttaaagta aagggagctt tagttcaaca gtcgatttct taaaatcggc
     1441 tttatttatg ggtgaaaatt acattaccgt tgtaaatccg cattttcagg actctacccc
     1501 ttctagagcg cctctcattg ttttagcgat taattattat tccccttatt atttttttga
     1561 aaatgttctt tgtaatataa ataatcaact ttatgttttg tggacattaa ttgagtcgct
     1621 aatattgcgg tgtaaattgt cgtcaatcct agtgctacta cggctaataa atttaaatta
     1681 cttttattaa tgctactgtt actattcatg aaagctcaac tcccttcttt aaaattagat
     1741 ttctaaatat caaagatttt tattcgaaca ccatcaattc tccccttgtt ttagggtgga
     1801 ggctggcaag ggggtttggg ggaagccccc atattgcgta gatccataag gcaaaagaga
     1861 aaatttatga acggaagctc tggcggtttt ggcggtataa tggctcgagc aagccgattt
     1921 cctgaattcc tcgttttagg tacgtcggct tggcgaaagt acagcaccgc caaaatttcg
     1981 gaatgcaagg agaaaagggt ggagattttt cggtggtctg tccgaaaaat actacccgaa
     2041 ccttgcgtgg agaaaagccc tttaggagat atagaaggga acacagaaaa aatgttgata
     2101 tatcaatgtt ttagttaagg taatgtgtca tagcttgtga cacgtatatg tgtcatagct
     2161 tgtgacacat agttgaatta agatgtgtca ttttttgctt gactatagtt catttttgac
     2221 ctatgatgtg tcataaatta tacatatagg tatcacagct gatgatacat aacaagggag
     2281 agaaggattc tatgccagca gaggtgcgta aattggacga gcagagaaaa attaatgttg
     2341 agttaacccc tgaacaactt caaatgcttc aaagacttat tgatcttgaa cagcaagaaa
     2401 acaaagacaa agagcaattt gaagagaaaa agaaaaatca taatttcatc cagctttatc
     2461 gtgacaacat gccagaatta agatggttaa tggcaaatca taacttcgca agttctttat
     2521 tattttttat cttggaacac atggataaca gaaatgcttt agcttgttct tatgcagttt
     2581 ttgaagatta ttttggcaaa tcaaggtcaa cagtttacag agcagtaaag cttttagagg
     2641 agaatggatt tttaaatgtt ttaaaaatgg gaacatcaaa cgtctatgta gtgaacgaag
     2701 accttgcttg gacagatggc aatgacaaaa agaaatttgc taaatacgat ggtaaaatcc
     2761 ttgtcagcaa aaaggaaaac aaggactttg aatacaaaag ccaatttgat aaatttaaag
     2821 cccttagaga acgtgaaaac ttaaaataaa gcgagtagat aactactcgc ctttctttat
     2881 ttcaagagca tatttgccct catttgaatc ttggtcaaca cttgtaaaaa caccaatatc
     2941 ttttattgga ataagagttc cagaacctaa atgaggtctc tttctatccc tcataaaaag
     3001 cattaattct ccctcttggt ctagtttctc caacttctca accaattgtt tcaccttcat
     3061 cagcgttcca tctccatccc tttttgcctt tgtggcttct ccattttttc taaagagtcc
     3121 tggactaatt tatcttgcct atcatccagg aacttgattg tacttcggtc tacatcattc
     3181 atcctcaaga acgtttttac tcggtcaagt tgagcgtgta aaactttgcc caccctctct
     3241 ttaaaaacag aaaactcctt gctgatctgc acgagttttc ccttcaattt tccgttctct
     3301 aatttcaatt cccgattctc attttggtaa gaccggttct caatttttag agtgtccacc
     3361 tgttgtttta actccttttt ctccttcaac tggtcattat atttattaac gagtccattg
     3421 tacttttcta tcaagtctcc atagttcttt acaatgtctg tttgcttcac tttttgcagt
     3481 gcttttttct ctgctaagga gtctaaattc tcttcaatat ctttgtagta atagcgaatt
     3541 ttatcttgtc caatttgcat atcagctttc agctgcgtaa cttctttttg agtcttttcc
     3601 aactctttct gcagattctc tgcttgtagc tgataagcca caccattggt cgcataatcg
     3661 actaggcttt tgtagtcttg ttccttcatt ccaacaagcc ctagaacctt tttagcttcg
     3721 atattcgcaa tcgatttcga tttatcatca atctcttgaa tatgccccat tgtccgctca
     3781 tatttctctt gggcttcctt ctccatgctt tgaaacgttt gagccttata tttagccgtc
     3841 tccacgtgtt tacggctcga cgagacccct ctttccaaat taaagtcatt ttgcttcagg
     3901 tactgattaa aatcgtcctg aagactcacc agttgctttt tctggccaaa gaaatcttta
     3961 gccgatagtc ggccatcctc cgttactgga acgaagccta cgtgcatgtg aggggttttc
     4021 tcgtcatagt gaacagtcgc atagattaaa ttcttttcac cgtatcgatc agcaaggaac
     4081 tcatgagccg tttcaaaata ccttttctgc tctttgggtg aaattttatc gaagaattcc
     4141 ttgtcagagg taattaagaa ttcagccagc ttaactgcat ctttacggat cttttttcca
     4201 gaggtcacat tgtcctcaat tacttggcta attttctcgt tgtaatcaat ctgcctttga
     4261 ccgtttatca agtcataatt cagatgagaa tcagctttat tgatgtcggg attcgtttca
     4321 ctttccctct ctctttggtt gtgaaattga atccctttta tcccatttga tttcattttc
     4381 tgcattcgga taatggagta actcatgggg tacgcaccct ttctgaaaat cgatagatta
     4441 atagaaagtg taacacacta cactttgcac gcaaagtttc atgtggtctg cgacgctaaa
     4501 agccaaacct tgggctttgc ccaaacccac tggggaactc ttccccagac ccccttaatc
     4561 gaactcccca acccctcaat ccttgagggg ctccctcgcc ggtaggcagc tcaaaaggtg
     4621 ttgccttttg agacaccaag agggtcttgg tgaagtgagt ctatcaactc cttatcctcc
     4681 cttcggtccg gtaccgt
//
