LOCUS       NZ_AP022891             5214 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0074 plasmid pMRY14-0074_5, complete
            sequence.
ACCESSION   NZ_AP022891
VERSION     NZ_AP022891.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203325
            Sequence Read Archive: DRR206365, DRR206389
            Assembly: GCF_021654935.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 382x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021654935.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:54:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,677
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,280
            CDSs (with protein)               :: 5,280
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 148 of 244
            Pseudo Genes (incomplete)         :: 110 of 244
            Pseudo Genes (internal stop)      :: 40 of 244
            Pseudo Genes (multiple problems)  :: 42 of 244
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022891.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0074"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0074_5"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            join(4796..5214,1..694)
                     /locus_tag="L6477_RS28180"
     CDS             join(4796..5214,1..694)
                     /locus_tag="L6477_RS28180"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_236798018.1"
                     /translation="MEEYWIPLLVDSEEKLQKNVDPRICNTGVQNTIDEGLSPCVDWVQ
                     VTFKNFNSTKKVFEILGLNPDMFSELPKGGLGYKKCTAFSNIRIFYDGREDMGVHLQIS
                     GSGCREYEHFGAYNWITLFQTIIGAGGQFTRVDLALDDVSKGNRKLFFTVESIARRVSK
                     ALLISKFKESDERLKRDIATGKSKGMSIYFGSEKSAIRVRMYDKKQEQGDKTPEDVEQW
                     VRTEIQTRDERAQMVAELIAFSKEETFGTVCKGILKHYLRFVVKGKDKNKSRWETAPFW
                     DKFLGEVQATKLTVAPKVRTIENSVHWIEKNVAPTLAMLHEAFGNDMDAIARIVNKGRA
                     NMNNQQKRMIETYHAEIAMIKKKANQQLTD"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            926..1429
                     /locus_tag="L6477_RS28185"
                     /old_locus_tag="BCM0074_p51"
     CDS             926..1429
                     /locus_tag="L6477_RS28185"
                     /old_locus_tag="BCM0074_p51"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="WP_236798019.1"
                     /translation="MRETTFEHIAKHIDRLEIDYDNGLILNRKPLLNECGYYRVYIGGK
                     YVLVHNVIAFVKYGTMAVGSLKQVQCNHMDGNKLNNRPENIVIVSARTNIRHAYGMNLI
                     NVNRGEKNHKAKINEDIVRDILHSHLAGVSRSAIAVKHGISNSVVQKVISGQTWSHVPL
                     YKRD"
                     /name="HNH endonuclease"
     gene            1686..2132
                     /locus_tag="L6477_RS28190"
                     /old_locus_tag="BCM0074_p52"
     CDS             1686..2132
                     /locus_tag="L6477_RS28190"
                     /old_locus_tag="BCM0074_p52"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236798020.1"
                     /translation="MNVKEAFEILKAEGLTSNIAIVRLWIRERKLKATMQEGYRKGGYD
                     IKEKDLEEFILSKIPPIRLEEYKRRKQLEKEKAKYKDDLELITKKVMDDILRNLEQTGD
                     SDKIAEIIAGDKEQLKHFTTSLIKQSVKTAVEIMGDLEKSKDSK"
                     /name="hypothetical protein"
     gene            2208..2390
                     /locus_tag="L6477_RS28195"
                     /old_locus_tag="BCM0074_p53"
     CDS             2208..2390
                     /locus_tag="L6477_RS28195"
                     /old_locus_tag="BCM0074_p53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236798021.1"
                     /translation="MALSAAALEAKREYQRQWRENNRERCNAYMNKWRKENPEKVKGYM
                     NAHWEKKAAEANVLD"
                     /name="hypothetical protein"
     gene            2456..3097
                     /locus_tag="L6477_RS28200"
                     /old_locus_tag="BCM0074_p54"
     CDS             2456..3097
                     /locus_tag="L6477_RS28200"
                     /old_locus_tag="BCM0074_p54"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236798022.1"
                     /translation="MKPQIIGHVYSGFAGMDDEYKALIAEHHKIKSQLGVYTQEYINEH
                     SLKTVVAIASLRGKYADKVNGLLDKLEDLYSVKKDVKAEPRDTNERLLYELQRSNDMRI
                     FDLKLRTNDPLELMALYDEYSSNEDFLVMLDARMHDVAPVTKSRLEAHIVASKRNEFLE
                     VIDLERRTITTLAKADSYPSNAVNGDAAIKFRSLSADLSNPILAGKEWVS"
                     /name="hypothetical protein"
     gene            3094..3240
                     /locus_tag="L6477_RS28205"
                     /old_locus_tag="BCM0074_p55"
     CDS             3094..3240
                     /locus_tag="L6477_RS28205"
                     /old_locus_tag="BCM0074_p55"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236798015.1"
                     /translation="MSKIIRAEIEAIRKAKETDHRNNEQQKVIQQMVNDEMAKVRKEFG
                     LQG"
                     /name="hypothetical protein"
     gene            4514..4771
                     /locus_tag="L6477_RS28210"
                     /old_locus_tag="BCM0074_p56"
     CDS             4514..4771
                     /locus_tag="L6477_RS28210"
                     /old_locus_tag="BCM0074_p56"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236798017.1"
                     /translation="MRKAEMKPFLNEMDSYKLSPTARLLYLVLEISGSSGKLLKNKEVS
                     EMTGINSSDTIKKAAAELESVKLIDRKIRNYGVEYTLLQK"
                     /name="hypothetical protein"
ORIGIN
        1 tgacgatgtg agcaaaggaa accgtaaatt gttttttaca gttgaaagca tagcgcgccg
       61 agtatcgaaa gctttattga tttctaagtt taaagagtcg gacgaacgcc ttaaacgcga
      121 tattgcaacg ggtaaaagca agggtatgtc tatctatttt gggtctgaaa agtccgctat
      181 acgagtgagg atgtacgata agaagcagga acaaggcgat aagacgccgg aagacgttga
      241 acaatgggta cgtaccgaaa tacagacgcg cgacgaacgg gcgcaaatgg ttgcggaatt
      301 aatcgcattc agcaaagagg aaacgttcgg aacggtttgc aaaggtattt taaaacacta
      361 tttgcggttt gtggtgaagg gaaaagacaa aaataagtcg cgttgggaaa ccgcgccatt
      421 ttgggacaag tttttaggtg aagtacaagc tacgaaatta acagtagcgc caaaagtaag
      481 gacgatagaa aattctgttc actggattga aaaaaacgta gcgcccacat tagcaatgct
      541 gcatgaagcg tttggaaacg atatggacgc gatagcgcga atcgttaaca aaggacgcgc
      601 taacatgaac aatcaacaaa agagaatgat tgaaacgtat cacgctgaaa ttgcgatgat
      661 aaagaaaaaa gccaatcagc aattaaccga ttgaccattt taaaaagtat tgttctgtat
      721 aaagtgtttc gccgcactca tacaggttgc tgcatttagt ttcgccccta aatacagcga
      781 aatgatttac ccctggaaaa ttcaagttat tacttacgta catattttat catatttcgc
      841 cgtgtgataa acatgtgaaa tctttccggg ggtttgtttt tagttacgtt gatcgttacg
      901 aaaaaaacca aaaggagaga atgacatgcg tgaaacaaca tttgaacaca ttgcaaagca
      961 cattgacagg ttagaaattg actacgataa cgggttaatc ctaaatagaa aaccgttatt
     1021 gaacgaatgc gggtattatc gcgtgtatat cggcggtaag tatgtactag ttcataatgt
     1081 tatcgcgttc gtaaagtacg gtacaatggc agtaggtagc cttaaacaag tacaatgcaa
     1141 ccatatggac ggaaacaaac tgaataatcg tcctgaaaat atagttatag tatcagcgcg
     1201 aacgaatata agacacgctt atggtatgaa tcttatcaat gtgaatcgtg gtgaaaagaa
     1261 ccacaaagca aagataaacg aggatattgt ccgcgacata ctacactcac atttagcagg
     1321 tgttagccgt tccgcaatag ctgttaaaca tggtatatcc aatagcgtgg tgcaaaaagt
     1381 tatttccggg cagacatgga gccatgtccc gttgtataag cgcgattaga agcagcagga
     1441 ctataactat ataaacctaa ttaaaagccg ttaggcgtca atttgaggcg tcaaacggct
     1501 atttttttat acagtcagat aatcacgcgt ctttttaggt gtctagaaag tccgttttgt
     1561 tgttatgtat ggttacgttt gctgagtagc aatatgcagg aaggacaaaa aaatatgatt
     1621 tgtgttattt gaatttttaa tgttataatt atgataataa cataagtaag aaatgaggaa
     1681 aaaagatgaa cgttaaagaa gcttttgaga ttttaaaggc tgaagggctc acatctaata
     1741 tcgcgattgt taggctttgg atacgagaaa gaaaactgaa agcgactatg caagaggggt
     1801 atcgaaaagg cggctatgac ataaaagaaa aggatttaga ggaattcatt ttgtcgaaga
     1861 ttccgccaat taggttagag gaatacaagc gaagaaaaca attagagaaa gaaaaggcga
     1921 agtataaaga tgatttggaa ttaatcacga aaaaagttat ggatgatatt ttgcgaaatc
     1981 tcgaacaaac aggagactca gacaaaatcg cggaaatcat cgcgggggat aaggagcaat
     2041 tgaaacactt cactacttct ctgataaaac aaagtgtaaa aacagcagtt gaaataatgg
     2101 gagacctaga aaaatctaag gatagcaaat aacgcattat gctgatgtta taaatcggat
     2161 aaatgcttta tatatatttc ctcaaaaatt ataagggagt tgataaggtg gcgctatcag
     2221 cagcagcact agaggcgaaa agggaatatc agcggcaatg gagggaaaat aaccgtgaac
     2281 gatgcaatgc ctatatgaat aagtggcgca aagagaaccc ggaaaaagtg aaggggtata
     2341 tgaatgcaca ctgggaaaag aaagcagcag aagcgaacgt attagattga tcgctacgaa
     2401 aattaaaaac aaattggagg ttttgtaaat gaaaaaagtg acggatatta aacagttgaa
     2461 gccacaaatc ataggacatg tgtattctgg ttttgcaggt atggacgacg aatacaaagc
     2521 cttaattgct gaacatcaca aaattaagtc acaattaggt gtatacacgc aagaatacat
     2581 caatgaacac tcgttaaaaa cagttgttgc tatcgcttct ttacgtggca aatatgctga
     2641 taaggtaaat ggactgttgg acaaactaga ggatttatac agtgttaaaa aggatgtaaa
     2701 ggcagagccg cgcgatacaa acgagcgtct tttatatgaa ttgcagcgtt ctaatgatat
     2761 gagaatattt gatttaaagt taagaactaa tgacccgcta gaattaatgg cgctatacga
     2821 cgaatacagc agtaatgagg attttcttgt tatgttagac gcaaggatgc atgacgttgc
     2881 cccggttact aaatcacgat tagaagcgca tattgtagca agcaaacgaa acgaatttct
     2941 agaagttatc gacctagaac gccgtacaat cacaacgtta gcgaaagccg attcataccc
     3001 gtctaatgct gttaatggtg atgcagcgat taaattccgt agtctatcag ctgatttaag
     3061 taacccgatt ctagcaggca aggagtgggt ttcgtgagta aaattatacg agcggaaata
     3121 gaggccattc gcaaagcaaa agaaaccgat catcgcaaca acgaacaaca aaaggttata
     3181 caacaaatgg ttaacgatga aatggcaaaa gttcgaaaag agtttggatt acaagggtag
     3241 gcagctattg ttgcttatcc ttttttattt gtatcgggat cacataggcg attgttcgtc
     3301 ttaataagtt cattttcaac ttctttcaac aagtgcagcc ctcgcgcgcg taaagaatgt
     3361 taacttttgt atggtttctt tttgttttcc atacgatttt cacaacgtgt ttttactatg
     3421 ccgaaccgct aaatgtcttg ctatcactag ctttatgaag ccttatgact tggtgatcca
     3481 tataatacaa gtccattgcc gtgtctaata tgcacgttta tgtaacctac cttttagaag
     3541 tggtcacgaa cattttgtat acttgaaccg ttctaatgcg tacagaagac atacagtatc
     3601 ccgacgcgtt ttacctatga aatgcattaa cgggattcta aacgcgctta aaaacgattc
     3661 tgaacagcca aacgcgtata cctgactgac acttaaactt tttgttgtaa ttccatttgt
     3721 taacgtacgc gtcaactacc cataacattg attatgttaa ctagaaaacc acaatgaaaa
     3781 accgccaatc ccttgtgttg caagactttc agcgttttaa ctcaaattat gtatatttat
     3841 cttccttaca gttcattcat tctcatgcat actaatgcgc tattttcgct atcatcgcta
     3901 ttattgcgtt tcttttatgc gcgattccat acatttgcta ttagcagcct tgctacatag
     3961 caatatgcaa atatagcccg gttatattcc tgttgcaaag tcatttattt tttgcgctgt
     4021 tgcttttgta tgacacggcg aaccatatcc ctaaaacggc tcattttgcc gcatacggac
     4081 ttttattttt tttggtgtgc aaatagataa aatccggcac tatacgcggc gcatttgatt
     4141 gtatgcagtc taggttatcg cgtagggctt atgctgcata tggcatatgt ctagcggtag
     4201 cggaacggct tgccgtgagc ataaggagca gacaggaagc acaagaccca aaccctttca
     4261 gtcctccaaa acccacgttt tggggatgcc taccggcgag ggatcgccta gcgcagcgcc
     4321 atgttttgta agttgatggt gtgcgaaata aagttgttat tttctacatg ttaaagcaga
     4381 taaaatgtat ttcggtgcaa gcattctaca cgtgggtgat ttatcgcgtg tgctatggtt
     4441 gtgcaaagtt tgcgaatatg tgctagtgta ctaggtgtaa agaaattaaa tacctgaatg
     4501 gaggtaaggc attatgcgta aagctgaaat gaagccattt ttaaacgaaa tggatagtta
     4561 caaattgtca cctaccgcaa gattgttata tctagtattg gagataagcg gttctagcgg
     4621 gaagcttttg aagaataagg aagtttccga aatgacagga attaattctt ccgacacgat
     4681 taaaaaagcg gcggcagaat tagaatcagt taagttgatc gataggaaaa ttcgaaatta
     4741 tggcgttgaa tacacgttgc tacaaaaata agaatgattt tagggggttt tgatgatgga
     4801 agaatattgg attccactac tcgttgatag cgaagaaaag ttgcagaaaa atgtagaccc
     4861 ccgtatatgt aacacggggg tacaaaatac aatcgatgag ggtttaagtc cttgtgtaga
     4921 ctgggttcag gtcactttta aaaatttcaa ttctacaaaa aaagtttttg agattttagg
     4981 cttgaatcct gacatgtttt ccgagttacc aaaaggcggt ttaggttata aaaaatgcac
     5041 agcgtttagt aacattagaa ttttttacga tggcagggaa gacatgggtg tgcatttaca
     5101 gatttcgggt tcgggttgtc gtgaatatga gcatttcggc gcttataact ggatcacatt
     5161 gtttcaaact attatcggtg caggtggaca atttacgcgt gtagatttag cgct
//
