LOCUS       NZ_AP022890            15480 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0074 plasmid pMRY14-0074_4, complete
            sequence.
ACCESSION   NZ_AP022890
VERSION     NZ_AP022890.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203325
            Sequence Read Archive: DRR206365, DRR206389
            Assembly: GCF_021654935.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15480)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 382x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021654935.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:54:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,677
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,280
            CDSs (with protein)               :: 5,280
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 148 of 244
            Pseudo Genes (incomplete)         :: 110 of 244
            Pseudo Genes (internal stop)      :: 40 of 244
            Pseudo Genes (multiple problems)  :: 42 of 244
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022890.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..15480
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0074"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0074_4"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            complement(414..1076)
                     /locus_tag="L6477_RS28125"
                     /old_locus_tag="BCM0074_p401"
     CDS             complement(414..1076)
                     /locus_tag="L6477_RS28125"
                     /old_locus_tag="BCM0074_p401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002035308.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000162379.1"
                     /translation="MTKKIIDFGQAEKKAKERDSKINSIYEKLEGSGGLSEEERVIMLQ
                     VLSKMSGGEEYFIGKKKKPTDRVRFVQIITDNINYLCKTGYLTNAEKAFLIDLTPYIEF
                     KTNILIECSNEDSEEVDADAATPSYLARKLGKDRSNLSHLMNGLLEKGVLAVAESGMTT
                     EDGRICSSRTWFVNPNVLCCSPKDGVDKATMKIFKKSLRNFKITGDKKKHNLPIYLF"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1297..2763)
                     /locus_tag="L6477_RS28130"
                     /old_locus_tag="BCM0074_p402"
     CDS             complement(1297..2763)
                     /locus_tag="L6477_RS28130"
                     /old_locus_tag="BCM0074_p402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901551.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_000901539.1"
                     /translation="MLGSISFNQSHQSSLSHNNRENIHGNPGIDPARSEENIYFVQKDI
                     RSVYKDVFQEAVDKYNEKQKRNDRKIENYYDKIHKDDKTHEQRELVVAIGEGKDDPKYR
                     EAKKEALKRYAEAFQERNPNLAVYNMVLHDDEANPHLHINYVPNFESSRGLTRRVGMDR
                     ALQQQGVEGTGRKLIAHWRERETAYIEQLAKEYIPNFERANVGSHKYMKVRQYKEYAEA
                     KSTIENQVEEKETKLQTIDDHLKNVEEKTNELEMTKKSLESDVVDTYKELEIVKQQVES
                     ENEKLQLIGQRHVELEKRVEQMQKELDSASDRVPNEPVKIPFLRKEVVVEVQDKMFGKA
                     EITKKQTRNYVLSPEQYQEVTKQVNAAVTIKKDHERLRETDFVKEYESLKMTADGWMKE
                     NRTLKQEKNQLQKEVGVLNREISSLKAHIKGLQTNIRVLYVQTKKVLKEQFKGFRESVK
                     NELGSKGVDNQFEREHKKEVNKHRGFDMER"
                     /name="plasmid recombination protein"
     gene            3395..4832
                     /locus_tag="L6477_RS28135"
                     /pseudo=""
     CDS             3395..4832
                     /locus_tag="L6477_RS28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053955.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /name="IS4 family transposase"
     gene            <4975..5450
                     /locus_tag="L6477_RS28140"
                     /old_locus_tag="BCM0074_p405"
                     /pseudo=""
     CDS             <4975..5450
                     /locus_tag="L6477_RS28140"
                     /old_locus_tag="BCM0074_p405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016136977.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /name="MarR family transcriptional regulator"
     gene            5826..8627
                     /locus_tag="L6477_RS28145"
                     /old_locus_tag="BCM0074_p406"
     CDS             5826..8627
                     /locus_tag="L6477_RS28145"
                     /old_locus_tag="BCM0074_p406"
                     /inference="COORDINATES: protein motif:HMM:NF026488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4573 domain-containing protein"
                     /protein_id="WP_236798014.1"
                     /translation="MGFRPHTTSQHSSQKAKFYIINIICNNINVDPVGPVAPVDPVAPV
                     GPVAPVDPVAPVAPVAPVDPVDPVDPVAPVAPVDPVDPVAPVDPVAPVDPVAPVAPVDP
                     VAPVAPVDPVAPVDPVDPVGPVAPVDPVAPVGPVAPVAPVGPVAPVGPVAPVGPVAPVA
                     PVDPVAPVDPVAPVDPVDPVAPVDPVGPVAPVAPVDPVGPVDPVAPVAPVDPVAPVDPV
                     DPVAPVDPVDPVGPVDPVAPVAPVDPVAPVAPVDPVAPVDPVDPVGPVAPVDPVAPVGP
                     VAPVAPVGPVAPVGPVAPVGPVAPVAPVDPVGPVDPVAPVAPVDPVAPVDPVDPVAPVD
                     PVGPVAPVAPVGPVAPVAPVDPVGPVDPVAPVAPVDPVAPVDPVGPVDPVAPVAPVDPV
                     APVAPVDPVAPVDPVDPVGPVAPVDPVAPVDPVGPVDPVAPVAPVDPVAPVDPVDPVAP
                     VDPVGPVAPVAPVGPVDPVAPVDPVDPVGPVAPVAPVGPVAPVAPVAPVAPVGPVDPVD
                     PVAPVDPVDPVAPVDPVDPVDPVAPVDPVDPVGPVAPVAPVDPVDPVDPVAPVAPVDPV
                     APVDPVDPVAPVDPVDPVGPVAPVDPVDPVAPVDPVAPVAPVDPVAPVAPVAPVGPVAP
                     VGPVDPVGPVDPVAPVAPVGPVGPVAPVAPVGPVDPVAPVDPVDPVGPVAPVAPVGPVA
                     PVAPVAPVAPVGPVAPVDPVDPVAPVGPVDPVAPVDPVDPVAPVDPVDPVDPVAPVDPV
                     DPVGPVAPVDPVDPVDPVDPVAPVAPVDPVAPVDPVDPVAPVDPVDPVGPVAPVDPVDP
                     VDPVDPVAPVAPVDPVAPVAPVAPVGPVAPVGPVDPVGPVDPVAPVAPVGPVAPVGPVG
                     PVGPVAPVGPVDPVAPVDPVGPVAPVGPVGKVSGGIGGNVGPINSGLKLLKKNGDGMLL
                     FDI"
                     /name="DUF4573 domain-containing protein"
     gene            complement(9329..10766)
                     /locus_tag="L6477_RS28150"
                     /pseudo=""
     CDS             complement(9329..10766)
                     /locus_tag="L6477_RS28150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053955.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /name="IS4 family transposase"
     gene            complement(11119..11565)
                     /locus_tag="L6477_RS28155"
                     /old_locus_tag="BCM0074_p409"
     CDS             complement(11119..11565)
                     /locus_tag="L6477_RS28155"
                     /old_locus_tag="BCM0074_p409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620148.1"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_000620148.1"
                     /translation="MIRLEKINADNYQKVFDLELVGEQENFVSSNMRSLAQAWVFYERA
                     KPYAIYNDDTIVGFIMFDYKIENRKVEIWRFMLGSAYQGKGYGKEALNKAIEFLKQENL
                     FDYIQINYVEENLVAKKLYQKVGFSETGEMEGNEVVMRLSIVKE"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(<11868..12077)
                     /locus_tag="L6477_RS28160"
                     /old_locus_tag="BCM0074_p410"
                     /pseudo=""
     CDS             complement(<11868..12077)
                     /locus_tag="L6477_RS28160"
                     /old_locus_tag="BCM0074_p410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002188100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /name="DUF421 domain-containing protein"
     gene            complement(12346..12867)
                     /locus_tag="L6477_RS28165"
                     /old_locus_tag="BCM0074_p411"
     CDS             complement(12346..12867)
                     /locus_tag="L6477_RS28165"
                     /old_locus_tag="BCM0074_p411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_000079496.1"
                     /translation="MAQTYWGSEVAKNLGIGSSTLRKYCLALEEVGYPFERGSNNSRIF
                     YHKDVAIIERLLTAMNKKNVTLEQAVNLAMTSVIENEIATVATDSVVDTEQIIVLSERI
                     ERLEQLNLELIQRLDQQSKFLQETDAQRIIREEQRDIQLMQVLREIQDSKRLIAASEQR
                     KSLWSRLFGK"
                     /name="DUF3967 domain-containing protein"
     gene            complement(13048..13659)
                     /locus_tag="L6477_RS28170"
                     /old_locus_tag="BCM0074_p412"
     CDS             complement(13048..13659)
                     /locus_tag="L6477_RS28170"
                     /old_locus_tag="BCM0074_p412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002188102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_000558980.1"
                     /translation="MHYIPFMYRYQYPYYVNVPIPMYNYGRQSVYCTFPNEIEHANRFD
                     FFRFSNGDGSISLKDYGSEPFVVNINEAAKQNNTYRTALWTGKHLQVTLMSINVGEDIG
                     LEIHPNVDQFLRIEQGQGIVQMGKSKDNLNFKRNAYDDFAIMIPAGTWHNVINTGNIPL
                     KLYSIYAPPNHSFGTVHVTKADALAVEEDYSHGNRNTVGY"
                     /name="cupin domain-containing protein"
     gene            14264..15214
                     /locus_tag="L6477_RS28175"
                     /old_locus_tag="BCM0074_p413"
     CDS             14264..15214
                     /locus_tag="L6477_RS28175"
                     /old_locus_tag="BCM0074_p413"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016107168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="WP_046199663.1"
                     /translation="MPKKVLLFTDLGIDDAFAILYTFFRNDIQLVGIVADYGNVSRENA
                     IKNINYLKYISGRKEIPVFLGASVPLTGILVQYFPEVHGKVGLGPIIPPEIPYPIYPMN
                     DIYKIINSNLDDLTIINLGRLSSLATSFILNLEAMRNVKEYICMGGAFFYPGNVTAVAE
                     ANFYADPYAANLILQHAKNLTIIPLNVTQHAIVTPEMVQQIDAFHRNTQDLAGLIIKPM
                     LDYYYNFYSKSNPGIGGSPMHDFVTIWYLLNPDAVRLSKVPIKVIPDQGEGFGQSIADF
                     RFVTNPGYKTHNIAFQFNYERFKRDIMETFLRKRL"
                     /name="nucleoside hydrolase"
     Typing          complement(415..1076)
                     /name="rep_cluster_1821"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1301..2763)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.598"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taggacgaat gatctaggcg gttcggcttg tatacttact agtccaagtc gatcggcatt
       61 gctttgcttt ttgtctgtcg gcttggcgag aaaagcatac aagccgaaaa aagttttgca
      121 aggacataat aaaaaagaaa agacggtagt ctttaggtga ggatgaacga accgactttt
      181 ttattatgaa ggtggagatt tttgagcgtt gtttgctcaa aaatactacc tgttccttgc
      241 ggaactttta gccctatata taagagtgag aagagcaaag taaagaaagt cagtaatatc
      301 aagggtttaa caagggtagg gtgtgggaaa attcccacac atgtgttgtt aaattcccac
      361 acctaaaaaa tggaaaataa taaaaaaaaa aagatgtcaa taacaaatga catctaaaat
      421 aaataaatcg gtaagttatg ttttttctta tctccagtta ttttaaaatt tctaagagac
      481 ttcttaaaaa tcttcatagt agccttgtct actccatctt taggggaaca acataaaaca
      541 ttaggattca caaaccaagt acgagaacta caaatgcgac catcttcagt tgtcattcca
      601 gattctgcaa cagctaaaac acctttttcc aataaaccat tcattaaatg agaaagatta
      661 cttctatctt ttcctaactt ccttgctaaa taacttggtg tagctgcatc tgcatctact
      721 tcttcagaat cttcatttga acattcaatt aaaatattag ttttaaactc tatatacggt
      781 gttaaatcaa tcaaaaaagc tttttcagca tttgtcagat atcccgtttt acatagataa
      841 ttaatattat cagtaattat ctgcacaaat ctcacccgat ctgtaggttt tttcttttta
      901 ccaataaaat attcttctcc accagacatt ttacttaata cttgtagcat aatgactctt
      961 tcttcttcag aaagtccgcc actaccttca agtttttcat aaatactatt aattttacta
     1021 tctctttctt ttgctttttt ttcagcttgt ccaaaatcaa taattttctt tgtcatatct
     1081 aagttaattc ctccttaatt gagggaatca acaaaaatac ctattttttg ctatttgatt
     1141 ataaatcaaa ttatgataat ctataatcag ataagtgaag tttaggtatt ttcgtaaaat
     1201 accctatgaa aagagtcatg ttcccgcatg gctctttttt tcattctatc atgtttttaa
     1261 caaccttaaa gataaaaaca aagtttccct gtttttttac cgttccatat caaatccacg
     1321 atgtttattc acctcttttt tatgctcacg ttcaaattga ttatctactc ccttgctacc
     1381 caattcattt ttaacactct ctctaaaccc cttaaattgc tcttttaaaa cttttttcgt
     1441 ttgtacatat aaaactctta tattcgtctg caaacccttt atatgagctt ttaacgagct
     1501 aatttctcta tttaatacac caacttcctt ttgcaactgg tttttttcct gtttcaatgt
     1561 tctattttct ttcatccaac cgtctgcagt catttttaaa ctttcatact ctttcacaaa
     1621 gtctgtttct ctcaaacgct catgatcctt tttgatagtg accgctgcat tcacttgctt
     1681 tgttacttct tgatattgtt ctggtgataa cacataattt cttgtctgtt tctttgtaat
     1741 ttcagctttt ccaaacattt tatcctgtac ttctactact acttctttac gtaaaaatgg
     1801 aattttaaca ggttcatttg gcactcgatc actagcacta tctaattctt tttgcatttg
     1861 ttctactctt ttttctaatt ccacatgacg ctggccaatt agctgcagct tttcattttc
     1921 actctctacc tgttgcttta ctatctctaa ttctttgtac gtatcaacaa catcactttc
     1981 caaactcttt ttcgtcattt ctaattcgtt tgttttctct tcaacatttt ttaaatgatc
     2041 gtcaatcgtt tgtaacttag tttctttttc ctctacttga ttttcaatag ttgactttgc
     2101 ttctgcatac tccttgtatt gacggacttt catgtactta tgtgaaccta cattagcacg
     2161 ttcaaaattc ggaatatatt ctttagccaa ttgctctata taagcggttt ccctttctct
     2221 ccaatgcgca attaactttc ttcccgttcc ttcgacacct tgttgttgca aagctctatc
     2281 cattccgaca cgcctcgtta atcctcgact actttcaaag ttcggtacat agttaatatg
     2341 taaatgtgga tttgcttcgt catcatgtaa aaccatgtta tagactgcca gattgggatt
     2401 ccgctcttga aacgcttcag cataccgttt taacgcttct ttcttagctt ccctatactt
     2461 cgggtcgtct ttaccttctc caatcgctac gaccaattct cgttgctcat gcgttttatc
     2521 atctttatgt attttgtcat agtaattctc aatcttacgg tcattccgtt tttgcttctc
     2581 gttatacttg tccaccgctt cttgaaagac atccttgtat acacttcgga tgtccttttg
     2641 aacaaagtaa atattctcct ctgacctagc tggatcaatg ccaggattcc catgaatatt
     2701 ttccctatta ttgtggctta atgaactttg gtgagattga ttaaatgaga tagaacccaa
     2761 cataaagtgc caccactttc cctagattaa ttgataccat aagtctagca aagcgacgaa
     2821 atggtgtcaa ttagagtaac cctatacgta ttttgacgct ttcgcatcaa aatcgatagg
     2881 ggtctgcgac gcttttcggc ttcgccgagc catgcttagc atggaaaaaa caaccttgga
     2941 ctctgtccaa acctgctggg gaaatcttcc ccagaccccc ttatccaact ccccaacccc
     3001 tcaaatcctt gagggcctcc ctcgccggtt ggcgtcccca aaacaatttg ttttggggaa
     3061 ctgccaagag gttggaaaat cagatgcacg agtcaattcc ttaatgctcc tttcaagaga
     3121 tcagcaaaaa aacgctgatc tctttcagtc cgcttaccat tgactcttac cacttatttt
     3181 tcttctccca ataatcactt tactaattat tcaatactac acttattaaa aagtaaaaag
     3241 tttttgagtt acttttttta aacgtatttc aaatgctttt aagttgtatt aggggtcacc
     3301 atacatgccc atcaacttaa gaattttcac tacccccaag tgacaaaaaa cgttattctt
     3361 tctaaaaagc tagctagaaa gggtgacatt ttttatgaat ctttcgattc aagatgaatt
     3421 acaactattt tctgaagaac tgtatcgtca tttaacccct tctcttttgg aagaactcgc
     3481 aaaagaatta ggttttgtaa aaagaaaacg aaagttttct ggaaatgaat tagctaccat
     3541 atgtatttgg gtaagtcaac gtacagcgag tgactcgctt gttcgactat gcagtcaatt
     3601 acatgcggcc acaggcactc ttatgagtcc agaaggactc aataaacgct ttgataaaaa
     3661 agcggttgaa tttttgaaat atattttttc ggcattatgg aaaagtaaaa tctgtgaaac
     3721 atcggccatt tcaagcgcgg cgctcacgta ttttcaacga attcgtattt tagatgcgac
     3781 gattttccaa gtaccaaaac atttagccca tgtatatcct ggatcaggtg gttgtgcaca
     3841 aacagcgggt atcaagattc aattagaata tgacttacat agcggacagt ttctaaactt
     3901 tcagataggt ccaggaaaaa ataatgataa gacctttgga acagaatgct tagacacatt
     3961 acgtccaagc gatttatgta tccgtgattt aggttatttt tcattagaag acttagatca
     4021 aatggatcaa cgaggtgctt attatatatc cagattaaaa ttaaaccata ccgtgtatgt
     4081 aaaaaatccg tttccagaat actttcgaaa cggaaccgta aaaaaagcaa tcacagtata
     4141 tccaagttga tttagaacat attatgaaca atttaaaacc cggtcaaaca tacgaaataa
     4201 aagaagctta cattggaaag aatcaaaaac tattcacacg tgtaattatc tatcgtttaa
     4261 cagacaaaca gatacaggaa cgtagaaaaa aacaaagtta cactgaaagt aaaaagggta
     4321 ttacattttc agaaaagagt aaacgattaa cgggtatcaa catatatgtc tcaaatacgc
     4381 ctgaaggagt tgttccgatg gaacaaatac atgactttta ctcccttcgc tggcaaatag
     4441 aaattatttt taaaacgtgg aaatccttat ttcaaattca tcattggcaa actatcaaaa
     4501 aagagcgatt agaatgtcat gtgtatggaa gacttattgc catctttatc tgttcttcta
     4561 cgatgtttaa gatgcgtaaa cttcttttgc aaaagaacaa aagagaatta agtgaatata
     4621 aagccattgg gatgattcaa gatcatgtat cgctgttata tcaagcgata cagagaaaca
     4681 cccaaaatct aacaaagatt ttaatccgtt tgttcgacct cctacagaag aatggacgga
     4741 aatctcatcg atatgagaag aaaactatct ttgatattat gggtgttgtc tatgagtata
     4801 atgggtttgg aaaacaaaag aaagctgcat aatttttaaa atgaaactcg ttagagttta
     4861 tttggtgtgt gtacttttca ataccaaaag tacattacgt gttttttagt ttgtttgaac
     4921 gaaagttcat tttgttttcg ttcttaagtt gatgggcatg tgacgctacc ccttatagga
     4981 aaaaagagaa aacctttaga tcgggtgagg tttgtgcaat taataatgga taatatcaat
     5041 tatttgactg agattggtta tttgagtagt aaagaagaag cttttttatt taaattagct
     5101 tcatttgtgg aatttaagac taatgttatt gtagaacgtg aaacaggtag tccagcatct
     5161 cccacatatt tgagtgaaaa gtttaaaata gctcgtcaaa atatttcagg tacaatgaat
     5221 actttgttaa aaaaaggagt attagctgtt gcgcaagcag gggtaacaac agaagatggt
     5281 cgggtgtgta caagtcgtac atggtttgta aatcctaata ttatgtgttg tagtccaaag
     5341 gatggtatag acaaagcaac acaacatatt tttagagatt cattacaaaa ttttaaattg
     5401 gaagaaaaag ggaaaaaaag aagtataaac ttcctgttta tttgttttaa agaatacaaa
     5461 tttctattgt attctttttt tttttgttat ttcccaattg ttaggtgtca tgaaaacatg
     5521 acacctaaca tcttatgaac ccttgatatt attaactttt ttcattttgt tcttctcact
     5581 cttatatata gagctaaaag ttccgcaagg aacaggtagt atttttgagc aaataacgct
     5641 caaaaatctc cacctttaaa aaaattatcc ttgcggaact tttttcggct tgtatgattt
     5701 tttcgccaag ccgacagaca aaaagcaaaa caatggttct cgacttgaac aaacaagtat
     5761 acaagccgaa tcgcttagat caatcgtcca aaaatagttt ttattctaaa atagatacgt
     5821 aattcgtggg gtttcgcccc cacacgacga gccagcactc tagccaaaaa gcaaaatttt
     5881 atataattaa tattatttgt aataatatta atgtagatcc agtaggtcca gtagctccag
     5941 tagatcctgt agctcctgta ggtccagtag ctccagtaga tcctgtagct cctgtagctc
     6001 ctgtagctcc tgtagatcct gtagatccag tagatccagt agctcctgta gctcctgtag
     6061 atccagtaga tcctgtagct ccagtagatc ctgtagctcc tgtagatcca gtagctcctg
     6121 tagctccagt agatcctgta gctcctgtag ctcctgtaga tccagtagct ccagtagatc
     6181 ctgtagatcc agtaggtcca gtagctccag tagatcctgt agctcctgta ggtccagtag
     6241 ctcctgtagc tcctgtaggt ccagtagctc cagtaggtcc agtagctcct gtaggtccag
     6301 tagctcctgt agctcctgta gatcctgtag ctccagtaga tcctgtagct cctgtagatc
     6361 ctgtagatcc agtagctcca gtagatccag taggtccagt agctcctgta gctcctgtag
     6421 atccagtagg tccagtagat ccagtagctc ctgtagctcc agtagatcct gtagctcctg
     6481 tagatcctgt agatccagta gctccagtag atcctgtaga tccagtaggt ccagtagatc
     6541 cagtagctcc tgtagctcca gtagatcctg tagctcctgt agctcctgta gatccagtag
     6601 ctccagtaga tcctgtagat ccagtaggtc cagtagctcc agtagatcct gtagctcctg
     6661 taggtccagt agctcctgta gctcctgtag gtccagtagc tccagtaggt ccagtagctc
     6721 ctgtaggtcc agtagctcct gtagctcctg tagatccagt aggtccagta gatccagtag
     6781 ctcctgtagc tccagtagat cctgtagctc ctgtagatcc tgtagatcca gtagctccag
     6841 tagatccagt aggtccagta gctccagtag ctcctgtagg tccagtagct cctgtagctc
     6901 ctgtagatcc agtaggtcca gtagatccag tagctcctgt agctccagta gatcctgtag
     6961 ctcctgtaga tccagtaggt ccagtagatc cagtagctcc tgtagctcca gtagatcctg
     7021 tagctcctgt agctcctgta gatccagtag ctccagtaga tcctgtagat ccagtaggtc
     7081 cagtagctcc agtagatcct gtagctcctg tagatccagt aggtccagta gatccagtag
     7141 ctcctgtagc tccagtagat cctgtagctc ctgtagatcc tgtagatcca gtagctccag
     7201 tagatccagt aggtccagta gctccagtag ctccagtagg tccagtagat ccagtagctc
     7261 cagtagatcc tgtagatcca gtaggtccag tagctcctgt agctccagta ggtccagtag
     7321 ctccagtagc tcctgtagct cctgtagctc ctgtaggtcc agtagatcca gtagatcctg
     7381 tagctcctgt agatcctgta gatccagtag ctccagtaga tcctgtagat cctgtagatc
     7441 cagtagctcc agtagatcct gtagatccag taggtccagt agctccagta gctcctgtag
     7501 atccagtaga tccagtagat ccagtagctc ctgtagctcc agtagatcct gtagctcctg
     7561 tagatcctgt agatccagta gctccagtag atcctgtaga tccagtaggt ccagtagctc
     7621 cagtagatcc tgtagatcca gtagctccag tagatccagt agctcctgta gctccagtag
     7681 atcctgtagc tccagtagct ccagtagctc cagtaggtcc agtagctcct gtaggtccag
     7741 tagatccagt aggtccagta gatccagtag ctcctgtagc tccagtaggt ccagtaggtc
     7801 cagtagctcc agtagctcca gtaggtccag tagatccagt agctccagta gatcctgtag
     7861 atccagtagg tccagtagct cctgtagctc cagtaggtcc agtagctcca gtagctcctg
     7921 tagctcctgt agctcctgta ggtccagtag ctcctgtaga tcctgtagat cctgtagctc
     7981 ctgtaggtcc agtagatcct gtagctcctg tagatcctgt agatccagta gctccagtag
     8041 atcctgtaga tcctgtagat ccagtagctc cagtagatcc tgtagatcca gtaggtccag
     8101 tagctccagt agatcctgta gatccagtag atccagtaga tccagtagct cctgtagctc
     8161 cagtagatcc tgtagctcct gtagatcctg tagatccagt agctccagta gatcctgtag
     8221 atccagtagg tccagtagct ccagtagatc ctgtagatcc agtagatcca gtagatccag
     8281 tagctcctgt agctccagta gatcctgtag ctccagtagc tccagtagct ccagtaggtc
     8341 cagtagctcc tgtaggtcca gtagatccag taggtccagt agatccagta gctcctgtag
     8401 ctccagtagg tccagtagct cctgtaggtc cagtaggtcc agtaggtcca gtagctccag
     8461 taggtccagt agatccagta gctcctgtag atcctgtagg tccagtagct ccagtaggtc
     8521 cagtaggtaa agtaagtgga ggaataggag ggaatgttgg acctattaat tctggattga
     8581 aattattgaa aaaaaacggt gatggaatgt tgttatttga catatagaaa cctcctatct
     8641 agtttattaa aaaaatatat tatatatagt aaatgtataa agatataaaa ttgattatta
     8701 taaatgtatt aattttttga cataaaaaag agatgaagga ccagttcatc tctttttccc
     8761 tattcaggtg aatttatgtc aatatttatt atatgtataa gttttaaaaa agttcttata
     8821 ttgaatttta taattaagta ttgttaaaaa gtgtaattat aaagtctgct gaaataaatg
     8881 tttcatttct tttaataggg gtcaccatat tattttggga aaattgtacg ctaaggacta
     8941 caaaacctta ttcttacagt atctttaaag gtaaatacat gttcagaaag atagaaacct
     9001 aagtttaatt gaacattgat ttgtttacat gctttatgaa catgattata gtcagaaaac
     9061 gtgttgatat tgttgagttt taccctctgt tcataaatat aaggttttct ggacagatat
     9121 ctcttagcgt acaggaaacg cttggaatgt gtccgaattg tgtctaaacg tacaattttc
     9181 ctaaaatggt atggtgaccc catgcccatc aacttaagaa cgaaaacaaa atgaactttc
     9241 gttcaaacaa actaaaaaac acgtaatgta cttttggtat tgaaaagtac acacaccaaa
     9301 taaactctaa cgagtttcat tttaaaaatt atgcagcttt cttttgtttt ccaaacccat
     9361 tatactcata gacaacaccc ataatatcaa agatagtttt cttctcatat cgatgagatt
     9421 tccgtccatt cttctgtagg aggtcgaaca aacggattaa aatctttgtt agattttggg
     9481 tgtttctctg tatcgcttga tataacagcg atacatgatc ttgaatcatc ccaatggctt
     9541 tatattcact taattctctt ttgttctttt gcaaaagaag tttacgcatc ttaaacatcg
     9601 tagaagaaca gataaagatg gcaataagtc ttccatacac atgacattct aatcgctctt
     9661 ttttgatagt ttgccaatga tgaatttgaa ataaggattt ccacgtttta aaaataattt
     9721 ctatttgcca gcgaagggag taaaagtcat gtatttgttc catcggaaca actccttcag
     9781 gcgtatttga gacatatatg ttgatacccg ttaatcgttt actcttttct gaaaatgtaa
     9841 tacccttttt actttcagtg taactttgtt tttttctacg ttcctgtatc tgtttgtctg
     9901 ttaaacgata gataattaca cgtgtgaata gtttttgatt ctttccaatg taagcttctt
     9961 ttatttcgta tgtttgaccg ggttttaaat tgttcataat atgttctaaa tcaacttgga
    10021 tatactgtga ttgctttttt tacggttccg tttcgaaagt attctggaaa cggatttttt
    10081 acatacacgg tatggtttaa ttttaatctg gatatataat aagcacctcg ttgatccatt
    10141 tgatctaagt cttctaatga aaaataacct aaatcacgga tacataaatc gcttggacgt
    10201 aatgtgtcta agcattctgt tccaaaggtc ttatcattat tttttcctgg acctatctga
    10261 aagtttagaa actgtccgct atgtaagtca tattctaatt gaatcttgat acccgctgtt
    10321 tgtgcacaac cacctgatcc aggatataca tgggctaaat gttttggtac ttggaaaatc
    10381 gtcgcatcta aaatacgaat tcgttgaaaa tacgtgagcg ccgcgcttga aatggccgat
    10441 gtttcacaga ttttactttt ccataatgcc gaaaaaatat atttcaaaaa ttcaaccgct
    10501 tttttatcaa agcgtttatt gagtccttct ggactcataa gagtgcctgt ggccgcatgt
    10561 aattgactgc atagtcgaac aagcgagtca ctcgctgtac gttgacttac ccaaatacat
    10621 atggtagcta attcatttcc agaaaacttt cgttttcttt ttacaaaacc taattctttt
    10681 gcgagttctt ccaaaagaga aggggttaaa tgacgataca gttcttcaga aaatagttgt
    10741 aattcatctt gaatcgaaag attcataaaa aatgtcaccc tttctagcta gctttttaga
    10801 aagaataacg ttttttgtca cttgggggta gtgaaaattc ttaagttgat gggcatgggg
    10861 tagcgtcagg aaaatgcgta tttacaacgc taaggaagtt ccaatcataa aaaaagccga
    10921 tttccagtct ctcaaggaat cgacttttta atttagattg ttgaactaaa gcacccttta
    10981 gtttaagtgt aactattcac aaaacaagaa tacaaaaagg gctagcagct gccaacctaa
    11041 aacgttctat ctatttgaaa tatactttta gctattcatc actgctgtta ggtaatgaaa
    11101 ctggaatata tttgacacct attctttcac tattgagagc ctcataacaa cttcgtttcc
    11161 ttccatttct cccgtttctg aaaaaccaac tttttgataa agtttttttg ccactaaatt
    11221 ttcttcaaca taatttattt ggatatagtc gaataaattt tcttgcttca aaaattctat
    11281 agctttgttt aatgcttctt taccatagcc ttttccttga tatgcactcc ctaacataaa
    11341 tcgccaaatc tctactttcc tattttctat tttatagtca aacattataa atcctacaat
    11401 tgtatcatca ttataaatag cataaggctt ggctctttcg taaaataccc aggcttgtgc
    11461 caacgatctc atatttgatg aaacaaagtt ttcctgttct cctacaagtt ctaagtcaaa
    11521 aactttttgg tagttatcag cgtttatttt ctctaatcta atcatcttat acatctccga
    11581 gttttgttta aatacttgtt cttgcgataa ttataacata atttaaaaga ctgggcaggg
    11641 gtttatatat aacactaaaa tttgttgaag tcttaatata tattttaaat aacttgcctt
    11701 tcactaacct gccccattag tttaagtaca ttttttcaca atctcaatag agtaagccta
    11761 ttggtacata attaaattat cgttaaatta ggacttcata atttctcata agacttgtat
    11821 caaaatgctt aacgttgtaa atccgcattt ttctgacgct accccaatac taacataaga
    11881 aaagcaatga ctgcatgatg agtttttata tttaaattaa atgccaaatt cgcaataata
    11941 cttccgatag agatagcacc aataaaatca aaagtattca tttcagcaat aatttgcttt
    12001 cctagtagtt tagctccgat aaataaaatc gaaaaagcta atagtgttct caaaataatc
    12061 tctatatgtt cagacacaag tagctaaccc cttttgaatt aagtgttcgt attataacaa
    12121 tttatataag gatagtatgt gtcattgtaa aagaatttat tatgttcaat agtattaaat
    12181 aattcaaaat taatgtcatg gttattcctc tgtagctccc cttgacatcg gggagctact
    12241 cccaatttta gaaattataa taacaccact tataagtaac aaaaaagctc actccttatt
    12301 tatttttata taggagtaag cttttctcta ttattaaatt ctaatttatt taccaaatag
    12361 acgactccaa agcgatttcc tctgttctga agctgctatt aatcgcttac tgtcttgtat
    12421 ttctcttaat acttgcatta attgaatatc tctttgttct tctcttatta ttctttgagc
    12481 atctgtttcc tgtaaaaatt tactttgttg atccaatctt tgaattaatt ctaaattaag
    12541 ctgttctaaa cgttctatac gttctgataa tactattatc tgttctgtat ctactacgct
    12601 atctgtagca acagttgcta tctcattttc tataacactt gtcattgcta gatttacagc
    12661 ctgttctagt gttacgttct tcttgttcat agctgttaat agccgttcta taattgctac
    12721 atccttatga taaaaaattc tagaattatt actaccgcgc tcgaaaggat acccaacttc
    12781 ctccagagca aggcaatatt tacgcaaagt acttgagcca atccctaaat tcttggctac
    12841 ctcagatccc caataagttt gagccatatt ttgtcctccc tctgcttcaa gctgttctcc
    12901 agttgctata tagtcttttc cttctttgaa aaggtaacac cttcttttta aaatttaaag
    12961 aagagttttc aattgttttt taagattaac aaaagtttat ttttttgctt tttaaaacac
    13021 agttatagat tttataataa atcttgatta ataaccaact gtattcctat tgccatgact
    13081 ataatcttct tccacagcca atgcatctgc tttagtaaca tgaacggtac caaatgaatg
    13141 atttggagga gcatatatcg aataaagttt taacggaata ttacctgtgt taattacatt
    13201 atgccatgtt cctgcaggta tcattattgc aaaatcatca taagcatttc ttttgaaatt
    13261 taaattatct ttactctttc ccatttgaac aatcccctga ccttgttcaa tacgtaaaaa
    13321 ttgatcaaca ttaggatgta tttccaaacc gatatcttca ccaacattaa tgctcattaa
    13381 ggtaacttgt aaatgtttcc ctgtccataa agcagtacga tacgtattat tttgctttgc
    13441 tgcttcattg atattaacaa caaatggttc tgatccataa tcttttaacg aaatacttcc
    13501 atcaccattg gaaaaacgaa aaaaatcaaa tctgtttgca tgctctatct cattaggaaa
    13561 agtacaataa acagactgtc ttccatagtt atacattggt attggtacat taacataata
    13621 aggatattga tatcgataca taaaaggaat atagtgcatt tttctcaatc cttcacaatt
    13681 ttataaaatt atcctatgca ctggttaagt caatgtactt aaaattcaaa gtgacttttg
    13741 taaagaaaga tttttagcct aaaacactta tttcattaaa tggaccttta acttaacaat
    13801 acaattgaag tttaaaaatc aaaagaaaaa aactatatca acaaagtttt tcatcaaact
    13861 ttgttttaaa attatcaatc ttttttatac aagattttat acattttttt gtactaatac
    13921 aggacattcg taaaccggaa aaaccatagt atctcaacaa tgtataaaat catgcatagg
    13981 atgacaaaca aagaaaaagc ggaatcctta ttatatcaat ggattccgct ttttccttgc
    14041 tgccgataat gagacgttat gtcaacttca catgaatatg atatacaaaa caaaaatact
    14101 attttagatg caaacacata taagatatct aaagttatag atgaacagaa tgtactattg
    14161 aagaatatat aaagaatata taaaatatat atcaaatata gtacaagcct attcattaca
    14221 tccttagtaa aaataaaatt ttatggcttt tggaggaaaa tatatgccca aaaaagttct
    14281 cttgtttaca gatttgggaa ttgatgatgc gtttgctatt ctgtacacct tttttcgtaa
    14341 tgatatccaa cttgtgggga ttgtagctga ttatggaaat gtatcgagag agaatgctat
    14401 aaaaaatatt aactatttga agtatatttc gggaagaaaa gagatacccg tatttctcgg
    14461 tgcttctgta cctttaacag gtatattggt tcaatatttt cctgaagtac atggaaaagt
    14521 aggactagga cctatcattc cacctgaaat cccttatcca atttatccca tgaatgatat
    14581 ttacaaaatt ataaactcaa atttagacga tcttacaatt atcaatttgg gaagactttc
    14641 ctcattggct acgtcattta ttttgaattt agaagcaatg cggaatgtga aagaatacat
    14701 ttgcatgggt ggagcctttt tttaccccgg aaacgtgact gctgtggctg aagctaactt
    14761 ttacgcagac ccttatgcag caaacttaat tctgcagcat gcgaagaatt tgacaattat
    14821 tccattaaac gtcacccaac acgcgattgt tacacctgaa atggttcaac aaattgatgc
    14881 atttcaccgg aatacacaag accttgcagg actcatcatc aaacctatgt tagactacta
    14941 ctataatttc tactctaaat cgaatcctgg cataggagga agccctatgc atgattttgt
    15001 aacaatatgg tatttgttga atccagatgc agttcgtctg tcaaaagtgc ccatcaaagt
    15061 aattcccgat caaggagaag gatttggtca aagtatcgca gactttcgtt ttgtgacaaa
    15121 tccaggttat aaaacacata atatcgcttt tcagttcaat tatgaaagat ttaagagaga
    15181 tattatggaa acatttttaa ggaaaagact atagcatatc ctaagtaaag ataaaaatac
    15241 atatttaaaa aagaattatt tatagttgga tgtattgtag ttgctatgag tttactaaaa
    15301 tagaacttcc tatatcgtga attatgtaaa taagccgtcc atatggacgg cttatttttg
    15361 tttagcctga ttttgtcctt gttttttggt ctactttttg ctttttggct agagtgctgg
    15421 ctcgtcgtgt gggggcgaaa ccccacgtat tccgtatcaa ttttggagta agcactattt
//
