LOCUS       NZ_AP022889            23000 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0074 plasmid pMRY14-0074_3, complete
            sequence.
ACCESSION   NZ_AP022889
VERSION     NZ_AP022889.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203325
            Sequence Read Archive: DRR206365, DRR206389
            Assembly: GCF_021654935.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23000)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 382x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021654935.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:54:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,677
            CDSs (total)                      :: 5,524
            Genes (coding)                    :: 5,280
            CDSs (with protein)               :: 5,280
            Genes (RNA)                       :: 153
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 148 of 244
            Pseudo Genes (incomplete)         :: 110 of 244
            Pseudo Genes (internal stop)      :: 40 of 244
            Pseudo Genes (multiple problems)  :: 42 of 244
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022889.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..23000
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0074"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0074_3"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            join(22423..23000,1..700)
                     /locus_tag="L6477_RS28020"
     CDS             join(22423..23000,1..700)
                     /locus_tag="L6477_RS28020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236798011.1"
                     /translation="MGNYKVLSKEEMSEQFTNDDELIQYILSGELSKKPFVVEKRELQE
                     TENKRAAEEGREPVHISDLPDVQITDFIVTKGFDFENKDDGNLIYPYYNEREDSFYDLL
                     FQGLKMLPAKGFFWLVDTTVENKPFGMYRTETSISKVMRKQNDFRFPNVQKLRDNPREG
                     KSYIDEKYFYYDFEEEKYIQVKIELDKEGNPVNDETEELYRRRETINYITVRFSKVLRE
                     YREFFIRKYEYIQSKSKDVNNTKTYIEIFTAYRELLTEAKQKLPVKLILGHVDSQEYVV
                     LSEHIDSKISEVLENIATIDEILKHENTEIIVFTQEEWAIKYKAIFERLGSSFKSGYLK
                     SVLDFITRRPEYEKQPKYTVSGYIVTKDEKGFVRRLIPNLGTVESRDFFTEFQSSDDKV
                     VEYERKLIEHLNHELVDYLNKKYPLN"
                     /name="hypothetical protein"
     gene            2325..3788
                     /locus_tag="L6477_RS28025"
                     /old_locus_tag="BCM0074_p302"
     CDS             2325..3788
                     /locus_tag="L6477_RS28025"
                     /old_locus_tag="BCM0074_p302"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_151154387.1"
                     /translation="MARKGAKRKKIKEIKQQEKQQKPTGEIRSKKKDGDHALNQMQFKS
                     RANESMYDKLAIEFLQNHLPKIMDIVPKEVREERGETGRTPGKLFDENLKTISTKTTYV
                     KRIKTYIKWHVINKGISSLGEITPESTKEFFSVMASNVGDGKGEYSTKTYDSYVNGVDK
                     FVHATTKSPESAKTKLEGREFSKPIESLSGMKSSELKKELKDMVPNYSQDNYKRGKEGG
                     YSVRDSQIIMKQAEKHMDIDKQLLVATFVYGGVRLDEARQFSLDCYNAQNNRLDMLKPH
                     MNKQDRPRVIMNVDQKLFNLVEKYKQEHQDVSPDANVFAKYSPNEVREIVKECCRLGKI
                     GYSGVHDLRKSFTAKSDTNLYKAVLKGQTTKKDLADTLMQQVSVDSRLNPMVPKKEWKM
                     RKTKSGKTKYYQKNVPAPGGGFVMEQKFTHEKLMNMNIENLVELFISEQLGHNKMETNM
                     EYRDKESRERRKDFRQRIKDMRKSGMKGL"
                     /name="site-specific integrase"
     gene            3833..4210
                     /locus_tag="L6477_RS28030"
                     /old_locus_tag="BCM0074_p303"
     CDS             3833..4210
                     /locus_tag="L6477_RS28030"
                     /old_locus_tag="BCM0074_p303"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_151154388.1"
                     /translation="MKKAYAYYCGLSIGKTIETQQKMVRKYAKSNNIQIIEEFEEVVIP
                     LNITKREELTKMLEKISSNKDIDYILVHSFEYITKDVMHMGYVMTALELDNGKTRIHSI
                     TEENDYDDPTKLMLLMIKIHG"
                     /name="recombinase family protein"
     gene            4434..5561
                     /locus_tag="L6477_RS28035"
                     /old_locus_tag="BCM0074_p304"
     CDS             4434..5561
                     /locus_tag="L6477_RS28035"
                     /old_locus_tag="BCM0074_p304"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_151154389.1"
                     /translation="MELTKELYTVKEVGEVLVQFGLVTRKGSNGYHTAHKLIQNGKIKS
                     EMPGARSQGRRVTKEDLEQYLKKHAPSYSYFGEKSKREAGLEAEIAALQSQIQQMQENA
                     NATEPEVDEFDKILGEMPKNIKNKIYQHFESLYIDKIQEEKENTKSLLAKREELMNEIS
                     VLKEHNKKLEKGIKPLKEEDFYPLETETKVLHVNIFGERKKAQEFYEDLKAVKGYHIQF
                     IEGSFKNKKREQQYTKLRITNEKKQVIHVPKAKKWETKYSAIEIIKVTSSKSHVSSEKN
                     YEITFKYKHMGIDSVIFVYDFSWGEDWDENINRSESIYCRYNKDDFEMSNVTYEAFQNR
                     LEHNEYTKKAVRDILERKLERIDDYKMLVPFIFTE"
                     /name="helix-turn-helix domain-containing protein"
     gene            5583..6062
                     /locus_tag="L6477_RS28040"
                     /old_locus_tag="BCM0074_p305"
     CDS             5583..6062
                     /locus_tag="L6477_RS28040"
                     /old_locus_tag="BCM0074_p305"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151154390.1"
                     /translation="MWRKIREKMSKNWLTKEEALNSGKLSSADESYRNAVNSGRRFLSK
                     TRCADIGLAVKKNEEPGAYVYSDEGVRPNFYMPIFERTDFFQTHQKLDKNEINREDNLH
                     LVPRSQMKDWGFKRYKGLPVEGYIEDENELEVVYNILETAEYQENKLKMLLEVNS"
                     /name="hypothetical protein"
     gene            6212..7084
                     /locus_tag="L6477_RS28045"
                     /old_locus_tag="BCM0074_p306"
     CDS             6212..7084
                     /locus_tag="L6477_RS28045"
                     /old_locus_tag="BCM0074_p306"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236798012.1"
                     /translation="MSNRYQHQTLYDLMQKQVMNLFQEGSHPMSLFGIETKTLKEAVPT
                     ELPMINDYSIDYGFLDEEDILHHIEFQTAYDKEDVYRFNIYHSLLKNMYKKKVKKVITY
                     IIFHSKIEPSGDKIEAMKLVEDDSQQFTPQVIFMRDEKSSKEAEQVWEKVRGCEHIELK
                     QEELAMLIYSMFGDMKRSFLEVAKELLTVSDKFINEDQQKMLAGCVISLSNRVLKKEEF
                     EHLEGMLNGMGQLLEKFGNDMVIERMIQTAAEALLQGINEKQVKAFTHLDDEQMKKAKE
                     LCKEMKKGQ"
                     /name="hypothetical protein"
     gene            complement(7278..7994)
                     /locus_tag="L6477_RS28050"
     CDS             complement(7278..7994)
                     /locus_tag="L6477_RS28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016105077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="WP_236798013.1"
                     /translation="MTGPTGPTGPTGATGVTGPTGPTGPTGATGITGPTGPTGPTGATG
                     ITGPTGPAATACCPCTNRLDNPGFDVPATPGFPVPGWAITGDVSEVGPPNVHSGRFLPD
                     GTFSILAASIGPGGIMTQSVGVGEGCCFTLSFAADVRDGGFLIASVSFPELGQGCPPNP
                     TTLGPLNIPHIVPVGNQPQSSFQHYVLVVCIPATVTTACITFQNTATGGAGATAFVDNV
                     VFQPTGGPCTSCSQNF"
                     /name="collagen-like protein"
     gene            complement(<8106..8321)
                     /locus_tag="L6477_RS28360"
                     /pseudo=""
     CDS             complement(<8106..8321)
                     /locus_tag="L6477_RS28360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016120346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /name="collagen-like protein"
     gene            9069..9587
                     /locus_tag="L6477_RS28055"
                     /old_locus_tag="BCM0074_p308"
     CDS             9069..9587
                     /locus_tag="L6477_RS28055"
                     /old_locus_tag="BCM0074_p308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151154374.1"
                     /translation="MDRFFKSQGCGCCGKFLPARAPEVNTIEFLPAYRNFWQNEFVTIP
                     NGQDIPFNNQSVAEAGGIVLLSPTTVFIPLAGDYEISYVITSVVFETINQEQQVTAILN
                     GAPVPGFQTSFGVITETEESCDQFSGTAILRIPAKSTFSLRNTSFNGSSISLCDLVESG
                     AALIIKKLS"
                     /name="hypothetical protein"
     gene            9758..10084
                     /locus_tag="L6477_RS28060"
     CDS             9758..10084
                     /locus_tag="L6477_RS28060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151154375.1"
                     /translation="MPKPPPKPPKPPSGGGGNTNNCNALCNAIISSLALVNALNTAMPG
                     GNYYVELNYNLSATQTVVLVVKSVNLQNCTIRGVELGTTNNITVSCAFAFANFVELKVT
                     SGPH"
                     /name="hypothetical protein"
     gene            complement(10308..10481)
                     /locus_tag="L6477_RS28065"
                     /old_locus_tag="BCM0074_p310"
     CDS             complement(10308..10481)
                     /locus_tag="L6477_RS28065"
                     /old_locus_tag="BCM0074_p310"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_192452099.1"
                     /translation="MTNNNKNIFSINDKEKVGEYLEDKRKQEKEVEETLRKNTETAVEN
                     AKQVIRKAFPNG"
                     /name="hypothetical protein"
     gene            11673..11891
                     /locus_tag="L6477_RS28070"
                     /old_locus_tag="BCM0074_p311"
     CDS             11673..11891
                     /locus_tag="L6477_RS28070"
                     /old_locus_tag="BCM0074_p311"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236797997.1"
                     /translation="MIERIQDGLFFWACSVYLPAIFCAFGISVHWVAIAFCFVAAINLS
                     KKLCGFLKKKIVAAVVKELQNDNNLKG"
                     /name="hypothetical protein"
     gene            12062..12280
                     /locus_tag="L6477_RS28075"
                     /old_locus_tag="BCM0074_p312"
     CDS             12062..12280
                     /locus_tag="L6477_RS28075"
                     /old_locus_tag="BCM0074_p312"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151154377.1"
                     /translation="MDIRIIDLLIGMACGFYLPPTVPVCGVNIHWVLLALGIILAIRFI
                     KKLGGFLKKKIVAAVVKELQNDNNFKG"
                     /name="hypothetical protein"
     gene            12880..13167
                     /locus_tag="L6477_RS28080"
                     /old_locus_tag="BCM0074_p313"
     CDS             12880..13167
                     /locus_tag="L6477_RS28080"
                     /old_locus_tag="BCM0074_p313"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236797999.1"
                     /translation="MDFVSINFRLKNYENESFQFLITKAPIEKRHFEIFIHGQLGHPAP
                     EETYSDNSMKKFKKYMEEERIDAIKEKILESIESNDEFAEQKFDFLLLLS"
                     /name="hypothetical protein"
     gene            13215..14105
                     /locus_tag="L6477_RS28085"
                     /old_locus_tag="BCM0074_p314"
     CDS             13215..14105
                     /locus_tag="L6477_RS28085"
                     /old_locus_tag="BCM0074_p314"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_236798000.1"
                     /translation="MEEVLKQYIAEKLFNKSKNTIDTYVRSIKQFLTWVEDSGLEVASI
                     KENKDGLTRLEMQDFVRYLEKQKKKSAQTVNKDWAALVSFMEWKGLKELTEDISLPQFE
                     KYTKTAPDFIEDTIINKIKREEYRKALRTGSWRDAAIINILIYFGIRIEELVTIDLSSV
                     ELSDRKGNLHIVGKRNKHRDIPFPKAEDNRSLREIISNYLSERLQMKTDEEALFLSNRG
                     NRLSKRAAQLIVEKHNVNCHAFRHTYITKLVRQGVDIPLIMSLTGHSSAEMIARYSKPN
                     EENRIDAQGKVSFDR"
                     /name="tyrosine-type recombinase/integrase"
     gene            14117..14359
                     /locus_tag="L6477_RS28090"
                     /old_locus_tag="BCM0074_p315"
     CDS             14117..14359
                     /locus_tag="L6477_RS28090"
                     /old_locus_tag="BCM0074_p315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236798001.1"
                     /translation="MFHSNTPDSDDFSMIEKLFAKYKFNSLYEQVRLRLLVVKDSFRDK
                     DEFENIVEEVLLISDQEGRKLDVNSFYFSKECKKT"
                     /name="hypothetical protein"
     repeat_region   14463..14752
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=14593..14622
                     /rpt_unit_seq="gttgaacaacaacataggatgtttttaaat"
     gene            14946..15131
                     /locus_tag="L6477_RS28095"
                     /old_locus_tag="BCM0074_p316"
     CDS             14946..15131
                     /locus_tag="L6477_RS28095"
                     /old_locus_tag="BCM0074_p316"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236798002.1"
                     /translation="MKKVGRPEVPEKKYRQKKIRFSKFEDFITTIVEEIDDEKSKNKIR
                     DLFIAERDKVISRTVI"
                     /name="hypothetical protein"
     gene            15485..17305
                     /locus_tag="L6477_RS28100"
                     /old_locus_tag="BCM0074_p317"
     CDS             15485..17305
                     /locus_tag="L6477_RS28100"
                     /old_locus_tag="BCM0074_p317"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236798003.1"
                     /translation="MKKIEEKLVLQDVQILNNARKQIEFLKLIYPDATFENGYKDGTIE
                     IRFLKRTSEGTRPLKSFNTWTLFETDIEYLQKTLSVYNGLPCCTYYSSYTFNRMNKTGE
                     KSISINNENALHTSILAMDFDGIGEVDFLVQKKRLLDIGIETVDVFTGHGYQSIILLKE
                     KSYDKELLKKFTELLLRKSFPVDSQIKDAARVLRLPYTYNCKAFLGGDKYYNPVNPEGI
                     QTFVYDTTTKRYDDKWVFEQLEGLEDAPNELEEFFDEFFPKSEEIIPVEVPASKVSDDI
                     IEEVKNEKKGVVFKTDALKEQINVEIKTKKEYEELYKHFNYIRLPEAIQNMLMGTPFSG
                     IGNDTLKFLVPFLKNHLGLSLEKQIETLQTWGELCKPVWDKEEIKKEVKRLNKYEIKAQ
                     YGEYTENMQKVYGDLVFDKWVNDDEIIIMNEIFEKIKDITDGAFRLYLSIKLLVHEEKR
                     SNFTNEEICERAEIAIRTFEKHIGHLRKQRLVGRKISNRSKGDKYEYYLNPYENTVKGF
                     TKIKASLIELLFLKGLNDAQITFYLYLKHMVGVSEESCWASQTYIAKELGKKSQTTISK
                     MTDELHKKKIIYKETKKVGKVVHSTYLLLK"
                     /name="helix-turn-helix domain-containing protein"
     gene            18123..18689
                     /locus_tag="L6477_RS28105"
                     /old_locus_tag="BCM0074_p318"
     CDS             18123..18689
                     /locus_tag="L6477_RS28105"
                     /old_locus_tag="BCM0074_p318"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236798005.1"
                     /translation="MDKNNVEFSEFGHLIAREAYYKNFYDKGLGEPLCQTIRGYAPDAS
                     ELEKFLDCKLGVDMIFEVDSIITTRLGTSYIGKRKRTAQERFVRNMRDSITISIQTGSG
                     HEGELFRLDLASHFIYGEYNGTDITDMIFITNTTLLADAIFDKKILFTTHENPNNGTIF
                     IAIKIEELDRNNIPYVRFKNKNKLH"
                     /name="hypothetical protein"
     gene            19455..20135
                     /locus_tag="L6477_RS28110"
                     /old_locus_tag="BCM0074_p320"
     CDS             19455..20135
                     /locus_tag="L6477_RS28110"
                     /old_locus_tag="BCM0074_p320"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_236798007.1"
                     /translation="MELLRKKDLEKRIGKPRTTINGWINDYNMYIPQTEDGKTTYFTKE
                     SVEVLLKIKELKEKGLEKPLIFEELQREGFTIQATEILEVPVSKEVFFSEDDNFQIFMK
                     KLFENTASKEEMQVLNKLLNKIDGRMTDIELEGSKRDRRMTDMKKTSIQYMKKVNELER
                     NFSEIEERIKDANNSDEKYYELEKENAILKTQLENFEKKLKDVEDRQYKQLEKKSFFQR
                     LFGK"
                     /name="MerR family transcriptional regulator"
     gene            20534..21754
                     /locus_tag="L6477_RS28115"
                     /old_locus_tag="BCM0074_p321"
     CDS             20534..21754
                     /locus_tag="L6477_RS28115"
                     /old_locus_tag="BCM0074_p321"
                     /inference="COORDINATES: protein motif:HMM:NF044815.1"
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="WP_236798009.1"
                     /translation="MTKTYVLSVANDNGNSEHKLTVNGTKVRQPNVYARISGPKFEKNN
                     TLEQAIQELHGNIDVTISSPAIADADNTRFLIGHAAMMGVGASKVKNMNLTKGKKHEED
                     LPIINTLGIIATKTVQKHFEETKEVKNNDMLEVTVNMSTCLPASVYKDATAKKFKERFQ
                     QNTHLVTVNIKSDLKVIVKINFGYVGVTAEGTSPLFEIIEDGQGNYRTGEMFKEFSEEY
                     KMEEVDGSYFADKNLMHVDIGDGTTELILTHGYDVKHSSGLKYGLGVALEEVSKKWEDK
                     KSYTRQDISDIAKSESHAFHSDIIEDLNGAPLQNLASDISHDVKQFLDELRAYQIQVMV
                     VYGGGSILLKNHLYNKLKEIAEEERFQLLWIPSKFSVDMNVNGLDTFNKIYMEELIAEA
                     AATAEEE"
                     /name="ParM/StbA family protein"
     gene            21826..22239
                     /locus_tag="L6477_RS28120"
                     /old_locus_tag="BCM0074_p322"
     CDS             21826..22239
                     /locus_tag="L6477_RS28120"
                     /old_locus_tag="BCM0074_p322"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236798010.1"
                     /translation="MTEAKKPTIQIKTSSPGCPDMVTKWSLEQQNVNDSVKELISIIAT
                     YTGYVELLDPETLRKIHAAFNNGTTSGISNDASTIEKQIHSETKTVEGKKQEENGTKDN
                     KKVKKEEPKQQNTDETNKVKPVLNRKRGTNFMK"
                     /name="hypothetical protein"
ORIGIN
        1 tccggtaaat gacgaaacag aagaattgta tagaagaaga gaaactatta attatataac
       61 tgtgcgattt tctaaggtat tacgagaata tagagagttt ttcatccgaa agtatgaata
      121 catccaaagc aaaagcaaag atgtaaataa cacaaaaaca tatattgaaa ttttcacggc
      181 atatcgtgaa ctattaactg aagcaaagca aaaacttcct gtgaaattaa ttttaggaca
      241 tgtagattct caagagtatg tcgttttaag tgagcatata gacagtaaaa tttcggaggt
      301 attagaaaac atagctacta ttgatgaaat tttaaagcat gagaatacgg aaattattgt
      361 attcacacaa gaggaatggg caataaaata taaagcgata tttgagagat tgggttcttc
      421 atttaaaagt ggatatttaa aaagtgtttt ggatttcata acaagaagac cagaatacga
      481 aaaacagccg aaatacactg tttcaggata tatagtaaca aaggatgaaa aaggcttcgt
      541 aagaagattg attcctaatt tagggaccgt ggaatcgaga gatttcttca ctgaattcca
      601 aagttccgat gataaagttg ttgaatatga aagaaaatta attgaacatc taaatcatga
      661 attagttgat tatttgaaca agaaatatcc actaaattaa gccgttctaa aaagaacggc
      721 tctttttatt tggttgcata taaaaatgta aaaaacggta taaaaaatga gttttttcac
      781 aaaaaggatt tttttccagt acaatatagg ttgaattata ttataagaac tcgaatacct
      841 tgagtttttc aagcaagtct tatcggacgt gctttttgag cattttataa gctaaccatg
      901 cttatttaac cctgttcaag tcaaaccaac acaatatatg aaaggagttt tctcattgaa
      961 aacatcagca aattttcaca ctaacagtcc gtaaggacat aatttcatgt accgtattaa
     1021 ttatagaaaa aataactttt ttaccacaag gatgaattca ttatcgttta gatattgaag
     1081 aaaaaaacaa aaaatataaa catgtggaat atgaaaaatc caaaatgatg cagcttaatc
     1141 ataataggaa aaaatatata ctcttggagg taaatagtta tgaaatcaat atttctatgg
     1201 taattcgtca aagtttttgg gagaaataaa attaaaaata gaaagggaag aagttaaata
     1261 caaaataaaa aagtctcttg cggaaacaag tggatattcg ttttgattag aaattcagag
     1321 aaaaatagtt caggacaaaa atgtatgaca cataactcct ggtgcaaatg gaaaataaaa
     1381 aacggatagg aacaatattg gaaaagattt taggataaaa agaaactgaa ataagaatta
     1441 ctgtcaacaa ttcccgtata tatttgccca ttgaaatcaa tggaagtatt tcttgcacac
     1501 catcgagtgt tcttgtgtca gcgacaaggt agaaatgcga atatcgtaca cacgactgta
     1561 ggggcataga cgttcttgtt aatcaggaaa atagccacac agattgttga aaaagaagag
     1621 agaaagactc tttcagggag gagaactaga caccactacc cctctgaaat ttttttaaaa
     1681 tttcagaggg gtagtggctt ttgtcacgtc agagacgtga caaaagaggg gatatcactg
     1741 agtgagcttg tatccaaagg ggtatttggc tgctgcttta gcagcagcca aagccctttt
     1801 tggatacaaa tgcgaacgga ttatggggta tgtgttttag tgagtcttgt gatggaaatg
     1861 ttgatgcggc gggcgttagc ccagccgtat cgacgttttc attgcaatgg cgaataaaag
     1921 aggaagtcga tttgaaatcg actgacgggg gttttatctt ttggggcacc cctctaccta
     1981 aacaggtaga gggggatagg aagtctgaaa cctctgggga gaatcccctt acgtttcagc
     2041 cgcgacgcac tttacgcggt gcatttgacc ttatgatttt gtggctcaaa attgtcggaa
     2101 aaaaatcccc tttttttgaa aagcactata taattgatga aatttaaaag tgaaaaagag
     2161 gtttgatttc accccttagg gtggaattaa tctaaaatgt tttccgattt ttatgtttga
     2221 atgtttatgt gaaaatgaaa aatgtttgaa ggacgagcgt gccccttgtg ggtatggttg
     2281 tctacttgtt ttggctttct tttttgaatg agaggtgaat atttatggca agaaagggtg
     2341 cgaaaagaaa aaagattaaa gaaataaaac aacaagaaaa acaacaaaaa ccaactggag
     2401 aaataagaag taaaaagaaa gatggggacc atgcattaaa ccaaatgcaa ttcaaatcaa
     2461 gagcaaacga gtcgatgtat gataagttgg cgattgaatt tttgcaaaac cacctaccaa
     2521 aaattatgga tattgttccg aaagaagtta gggaagaaag aggagagaca ggaagaactc
     2581 ctggaaaatt gtttgatgaa aatctaaaaa caattagtac aaaaacaacg tatgtaaagc
     2641 gaatcaaaac atatataaag tggcatgtaa ttaacaaagg aatctcttct cttggggaaa
     2701 ttacaccaga atctacgaaa gaattttttt ctgtaatggc aagcaatgtg ggggatggaa
     2761 aaggggaata tagcaccaaa acctatgata gctatgtaaa cggggtcgat aaattcgtac
     2821 atgctactac aaaatcacca gaatcggcaa aaactaaatt agaaggaaga gagtttagca
     2881 agcctataga gtctttatct gggatgaaaa gtagcgaatt gaaaaaagaa ttgaaagata
     2941 tggttccgaa ctattcacag gacaattata aaagaggaaa agaaggtgga tatagcgtta
     3001 gagactctca gattatcatg aaacaggcgg aaaagcacat ggacatagat aaacaactgt
     3061 tggtcgcaac gtttgtatat ggaggtgtga ggcttgatga agcgcgtcaa ttttctttgg
     3121 attgttataa cgcccaaaat aatagattag acatgctgaa accacatatg aacaaacagg
     3181 accgtccgag agttatcatg aatgtggacc agaagttatt taatttagta gaaaaatata
     3241 aacaagaaca ccaagatgtt tccccagatg ctaatgtttt tgctaaatat tcaccgaatg
     3301 aagttcgtga aattgtaaaa gaatgctgta ggttagggaa aataggatat agtggcgttc
     3361 acgatttgcg taaaagcttc acggcaaaat cagataccaa tctatacaaa gcggttttga
     3421 aggggcaaac cacgaaaaaa gacttagcgg atacactgat gcagcaagtt agcgtggact
     3481 cacgtttgaa tccaatggtt ccaaagaaag agtggaaaat gagaaaaacg aaaagcggaa
     3541 aaacgaaata ttatcaaaaa aatgtaccgg ctcctggtgg gggatttgtt atggaacaga
     3601 aatttaccca tgaaaaacta atgaacatga atatcgaaaa tctagttgaa ttgttcattt
     3661 ccgaacagtt aggacacaac aaaatggaga caaatatgga gtatagggat aaagaatcaa
     3721 gggagcgtag gaaggatttt agacagagaa tcaaagacat gagaaagagt gggatgaaag
     3781 gattataagt tgaaaaagat agatttaatt ttaaattata ggaggggatt aattgaaaaa
     3841 agcatatgcc tattattgtg ggcttagtat tgggaaaacc atagaaactc aacaaaaaat
     3901 ggttcgtaag tacgctaaaa gtaataatat tcaaatcatc gaagaatttg aagaagtagt
     3961 aataccatta aatataacaa aacgagaaga actaaccaaa atgctcgaaa aaataagttc
     4021 aaataaggat attgattaca ttttagtaca tagttttgaa tatattacaa aggatgtcat
     4081 gcatatgggt tatgttatga cagcattgga attggataat gggaaaacca gaatacattc
     4141 aataaccgaa gaaaacgatt atgatgaccc tacaaaactg atgttactta tgataaaaat
     4201 tcacgggtaa ttagaatcca agaagagtaa ttttctactc tttttttgtt ttattcctat
     4261 tggactttta tccaaaaggg tgatttaatt gattgtaaga attcttttgg ataaaaatcc
     4321 aaaaggagaa ggtggaaaat cttttttata gaaattaata tatagaatga atcggtctat
     4381 ttttttatga ttgatttgga tttaaatcca aatgaagaga gagggacaat agaatggaac
     4441 taactaaaga gttatatacg gtaaaagaag ttggagaggt tcttgtgcag ttcggtttag
     4501 taactcgaaa aggctccaac gggtatcata ccgcacataa gttaatacaa aatggtaaaa
     4561 taaaaagtga aatgccaggt gcgaggagcc aagggagaag ggttacgaaa gaggatttag
     4621 aacagtattt gaaaaagcat gctccatctt atagttattt tggagaaaaa tccaaaagag
     4681 aggctggatt ggaggcggaa atagcagcat tgcaatctca gatacaacaa atgcaagaaa
     4741 atgcaaatgc aactgaacca gaagttgatg aatttgataa aatactgggt gaaatgccca
     4801 aaaatataaa gaataagata tatcaacatt ttgagagttt atatattgat aaaatacaag
     4861 aggaaaaaga gaatacgaaa tctctgcttg ctaaacgtga agaattaatg aacgaaatct
     4921 ctgtattaaa agaacataat aaaaaactag aaaaggggat taaaccgtta aaagaagaag
     4981 acttttatcc tttagaaacc gaaacgaagg tcttgcatgt aaatatattc ggagaaagaa
     5041 aaaaggcaca agaattttac gaagatttga aagcggtaaa gggatatcac attcaattta
     5101 tcgaaggttc gttcaagaac aagaaaagag aacaacaata taccaaatta agaattacga
     5161 atgaaaaaaa gcaagttatt catgtaccca aggcgaaaaa atgggaaaca aaatattccg
     5221 cgattgaaat tataaaagta acgagtagta aaagtcatgt ttcttctgag aaaaattatg
     5281 agataacttt taagtacaaa cacatgggaa tagattctgt tatttttgtt tatgatttct
     5341 cctggggaga ggattgggac gaaaatatca acagaagcga aagtatatac tgtagataca
     5401 ataaagatga ttttgaaatg tctaatgtga cgtacgaggc atttcaaaat cgactagaac
     5461 acaatgaata tacgaaaaag gctgttcggg atattttgga acgaaagtta gaaagaatag
     5521 atgattacaa aatgttagta ccgtttattt ttacggagta atacataaaa ggggataatg
     5581 aaatgtggag aaagattcga gaaaaaatga gtaagaattg gttaacaaaa gaggaagctt
     5641 tgaattctgg taagttgagt tcagccgatg aaagttaccg aaatgcagtc aattctggtc
     5701 gtcgtttttt aagtaaaacg cgttgtgctg atataggact agctgttaag aaaaatgaag
     5761 aaccaggtgc atatgtttat agtgatgaag gtgtacgacc taatttctac atgccgattt
     5821 ttgaaaggac tgactttttt caaacacatc aaaaactaga taaaaatgaa attaacaggg
     5881 aagataattt acacctggtc ccgcgcagcc agatgaaaga ttggggattt aaacggtaca
     5941 aaggacttcc tgttgaggga tacatagaag atgaaaatga attagaggtt gtttacaata
     6001 ttttagaaac ggcggagtac caggaaaaca aattgaaaat gttgttggaa gttaactctt
     6061 aattcatatt gaatttttaa aaggaaataa atcgactctt ttgttttcat gataaaatca
     6121 ttgaaattgc aaaggagaat gccatgaatt aataaatgta gaaatgcttc agaaacaggg
     6181 aaaataaatg gcggaaagga ataatataat tatgagtaac cgatatcagc accaaacgct
     6241 ttatgatttg atgcaaaaac aagtgatgaa tttgtttcaa gaaggtagtc acccaatgtc
     6301 attgtttgga attgaaacga agacattgaa agaagctgtg cctacggaat taccaatgat
     6361 aaatgattat tcaattgatt atggtttttt agatgaagag gatattcttc atcatattga
     6421 gttccaaacg gcatatgaca aggaagatgt atatcgattt aacatatacc attcactttt
     6481 aaaaaatatg tacaaaaaga aagtcaaaaa agtcataact tatatcattt tccattcgaa
     6541 aatagaacca agcggtgata agattgaagc aatgaaatta gtggaagatg atagtcaaca
     6601 gttcactcct caagtgattt ttatgagaga tgaaaaatcc tctaaagaag ccgaacaggt
     6661 ttgggaaaag gttcgaggtt gtgaacatat tgaattaaaa caggaagaat tagccatgct
     6721 aatatatagt atgtttggag atatgaaacg gtcttttctt gaagttgcaa aggaactact
     6781 taccgttagt gacaagttta taaatgaaga ccaacaaaaa atgcttgctg gttgcgtaat
     6841 atcattgtca aatcgggtgt taaaaaagga agaatttgaa catttagaag ggatgttgaa
     6901 tggaatggga caacttttag agaagtttgg aaatgatatg gtgattgaaa gaatgattca
     6961 aacagctgct gaagcacttt tacaaggtat aaatgaaaaa caagtaaagg cttttacaca
     7021 tttggatgac gaacaaatga aaaaagcaaa agaattatgt aaagagatga aaaaaggaca
     7081 ataaaaataa tgtaaagagc ggattcccgc tctttttttg ttgcgaatac ccctcaggat
     7141 ttttaaaata gggttctttt tttgtcagat acctggtacc tatcaagcta gttaattaga
     7201 tgccctaaca tcttcttgga aaataatctg ttttttagga ccttagcgaa accaaaaact
     7261 gtaaacgcat caatacatta gaaattttgt gagcatgaag tacatggtcc accagtaggc
     7321 tggaatacaa cattatcaac aaaggctgtt gcacctgctc caccagtcgc agtgttttgg
     7381 aaagtaatac atgctgttgt aacagtagca ggtatacaca caacaagaac ataatgttgg
     7441 aaacttgatt gtggttgatt tccaacaggt acaatgtgtg gaatgtttaa tggtccaagc
     7501 gttgtggggt taggagggca tccttgccca agctctggaa acgatacaga agcaataaga
     7561 aatcctccgt ctcttacatc cgcagcaaag gaaagagtaa agcaacaacc ttcgcccaca
     7621 ccgacagatt gagtcatgat tccgccaggt ccaatagaag cagctaagat actaaatgtt
     7681 ccatctggaa gaaaacgacc actgtgcaca tttggaggtc ctacttctga aacatctccc
     7741 gttatagccc accctggaac cggaaagcct ggtgttgcag gtacatcaaa tccagggtta
     7801 tcaagacgat tcgtacatgg acaacatgcc gtagcagctg gtcccgtagg tccagtaatt
     7861 cctgtagccc cggttggtcc agtcggtccc gtaggtccag taattcctgt agccccggtt
     7921 ggtccagtcg gtcccgtagg tccagtaact cctgtagccc cggttggtcc agtcggtccc
     7981 gtaggtccag taattcctgt agccccggtt ggtccagtcg gtcccgtagg tccagtaatt
     8041 cctgtagccc cggttggtcc agtcggtccc gtaggtccag taactcctgt agccccggtt
     8101 ggtccagtcg gtcccgtagg tccagtaact cctgtagccc cggttggtcc agtcggtccc
     8161 gtaggtcccg taggtcccgt aggtccagtt ggtccagttg gtccagttgg tcctgtagcc
     8221 ccggttggtc ctgtaggtcc cgtaggtcca acttggggta gaggtacagg gggaagaaaa
     8281 cactcacacg gaatttcaaa tctcttttta aaattaccca tctacatatc atcctatctt
     8341 acaccaactt acaccctata acttatgaat tagtaaacga acaagtataa gaaaagaacc
     8401 taaaaaaaga aaaatataaa cagcaagtcc ttatatcaca agcatagatt aatttcataa
     8461 tctataaggg ggaataaatt tcatcaaaaa acgtataaat aacgtacagg aggttcctgt
     8521 tcctcctgtt gttcctggtg taccttgtga aggatagagc taatattcat gggcacaaat
     8581 attagctcta tcgtatacgc gcgccctata ataaaaataa aaagtgaatt gttagtgggg
     8641 tttctttcta tgtaaaaact acataattaa gatgatttct ttatgtatca attaaaaagg
     8701 aacatgtagc tgttaattag aataaaatat tacagacatg tttttcacgt tatcattaaa
     8761 agctgaccta atagcattgg gttggttttt ttatgctgga attttaattg atttttattt
     8821 ttttaaagaa aaataatgaa accgcttccg ttatatgcat atactgtact gacataaaaa
     8881 tctctccaaa aaacactata taaaaagaga tgagttggtc cttcatctct tttttaaatt
     8941 cagtaacaaa cacgaacctt gaaaccaact tcagggcttg tttgttttgt cggacaaaca
     9001 tccaagctga attttttgga ttgaatatac ataattatat ccatttcaaa aaaaggaagt
     9061 gaattatttt ggaccgattt tttaaatcgc aaggttgcgg ttgctgtggt aaatttcttc
     9121 ctgctcgtgc acctgaggtt aacacaatag aatttttacc agcttatcgt aacttctggc
     9181 aaaatgaatt tgttactata cctaacgggc aggatatccc atttaataac caaagtgtag
     9241 ctgaagcagg aggaatcgtt ttattatcgc ctactacggt attcattcca ttagctggag
     9301 attatgaaat tagctatgta attacaagcg ttgtttttga aacaataaat caagaacagc
     9361 aagtaacggc tatcttaaat ggagctccgg ttcctggttt tcaaacatct tttggagtaa
     9421 taacggagac tgaagaatca tgtgaccaat tttcaggaac agctatttta agaatccctg
     9481 ctaaatcgac tttttcacta agaaatacaa gcttcaatgg aagttctata tctctttgtg
     9541 atttagttga aagtggtgca gcactaatca ttaaaaaatt gagttaaaag cttagaaaaa
     9601 ggacaaggtg ccttacctgt cctttttaat tttaggtaaa catatctgta gtgcataaaa
     9661 ttgttaatta ttcataggac aaactacaca ggcaattatt ttcgcggaga atattatgta
     9721 ttgattgtaa atttcattga aaaggaactg atgaaaagtg ccaaaaccac caccaaaacc
     9781 cccgaaacca ccatcaggcg gcggaggaaa tactaataat tgcaatgctt tgtgcaatgc
     9841 aattattagt agcttagctt tggttaatgc cctaaataca gctatgcctg gaggcaacta
     9901 ctacgtagaa ttaaattata atttaagcgc aacgcagaca gtggtgcttg ttgttaaaag
     9961 tgttaatctc caaaattgta caatcagagg ggtagaacta ggaactacaa ataatataac
    10021 agtaagctgt gcttttgcat ttgctaattt tgtagaactt aaagtaactt ccggaccaca
    10081 ctaattaaaa atacagtccc caccttaatt ggtgcatata agtaggtggg gaagggataa
    10141 ttcaccttgt taaagataaa agaatgaata tgattttaaa acatacatat aattgattaa
    10201 gttgatacaa aattccctcg actgttaaat aagaaaaaga gatggaagcc tactccatct
    10261 ctttttctta ttgtaaatga atgcagagaa gatgtaccgc aaaagaatta tccattcggg
    10321 aatgcttttc gtattacttg ttttgcgttt tccactgcgg tctctgtgtt ttttcgtaac
    10381 gtctcctcga cctctttttc ttgttttctt ttatcttcta gatactctcc tactttctct
    10441 ttatcgttaa tagaaaatat gtttttatta ttgtttgtca tggtgtttcc tctatttcat
    10501 tgtataaaat cataaagaaa agacactcta aagtgccttc ctccgacttg aaccacttta
    10561 attttaataa cattggactc ccatccaaat agtattttat catatatccc atgtattgga
    10621 aacgcattca ttaacttaat taattatttt gtccgcaact tctttataat tattaaactc
    10681 ttcatttttt tatctgcaag aggaagtttc ctagtcctac atgtgttgag taaacaggga
    10741 agtcatatgt ttattgtaga ggtatttaag gttgctccga ttgatggaat aattagagaa
    10801 gaaatagtta taatgtaata tctagtgagc aaggtattgg ttatgcgaaa tgataaagaa
    10861 taaaagatgt tggaatgtgc gtgtgtgtaa gtgccttttt ttgttataat gaaagaatgc
    10921 aataaaatat gcaataatca gattaattgt tcactttgca aaaaaaatat taaatatttt
    10981 tatcgtacca cttgtatttt ctgtgattag tagggttata gaaggaaaca acaagttttt
    11041 cattaataaa ggggggcgga gtgagaaaaa agtgtaacgc gattagtaat aatggttagt
    11101 agaaagaggg ttaaccgaaa agaacatatt tttttaaagt aataaaggac aaactaataa
    11161 attatttaat gtagtaatag tagaacaagg tactaccaat aaagtgttta attttaagaa
    11221 gaaaaccggc tatactagta gataagaagt attttttctg aaaatagaaa gtttttctgt
    11281 aaacggaaat aggatggtgt agaatattta tagaagaact ttttcccaaa gtagaaattg
    11341 aatcgtctat aataaaaaag agggtaatta tttccatgaa atagaaatag cctcttggcg
    11401 aatggtgctg aaatttggtg tgtatcacag gtcttgccat ccatttttac atgcaccgat
    11461 aaaaacggca aaaaaaatag ccgtttacgg cttggtttct ttagtttgcg ctttccaggg
    11521 tcttccctgt ttatatagat agaaagttgt tgacacggaa tagctaaatg gttacgttaa
    11581 gtcgtgtcag gatagaaaaa tgctgaaggt ccttgaaaat aaagaatagt gtactcttta
    11641 tagttcctct cagtggaaag gagaaaacca atatgattga aagaatccaa gatggactat
    11701 tcttttgggc atgtagtgtt tacttgccag caattttctg cgcatttggt ataagtgtgc
    11761 attgggttgc gatagccttt tgtttcgtag ctgcgattaa tctctcaaag aaactttgtg
    11821 ggtttctaaa gaagaagatt gttgccgcag tagttaaaga gttacaaaac gacaataatt
    11881 taaaagggta acaaaggtaa cacacatatt gattgggact tggtgggtcc ttggctggtc
    11941 ttgaattttg gtagattcga gacttgccta caatatgtaa ttaaaaaatg ttgaaaggtt
    12001 attagtctga caatgaaata tgcaattctt gtcattcctt tcgacagaaa ggagaacaaa
    12061 catggatatt cgaataatag acttacttat tgggatggcg tgtggttttt atctgccccc
    12121 aactgtgcct gtctgcggtg tgaatataca ttgggtttta ttagccctag gtatcattct
    12181 agcaattcgc ttcatcaaga aacttggtgg gtttctaaag aagaagattg ttgccgcagt
    12241 agttaaagag ttacaaaatg acaataattt caaagggtaa catacatata aggacttgat
    12301 gggtccgtgg tgagtcttga attggtagat tcgggactcg cctgttatat gtactacaag
    12361 tattacgttt aataaataac tttataactt gtaaactaat catagcaaac aaaaacttgg
    12421 aattcaacaa aaacctaggg aaaagtgagc ttttaccaag tatgtttgct ggtaaaagtt
    12481 aaattcctca tttattttgt tggtaaaagt gttaaatcct tcattaaaca gtatattcac
    12541 ctgcaaaaaa tatatacagg cgaatttaaa ggattatatt tttttaacag aaatactaca
    12601 cagaattaga attccgtgca gttattgtgt taaaatgaaa atgtaaggga aaaataaagc
    12661 attgaaataa aaacatttta catttttttg caaacgtata actgtgaaaa taaactgcac
    12721 agaattcgaa actgtatttt tttaaccaaa agcaagtccg ataatcattt tattttctgg
    12781 tgaaagggat atttaattta tgaaaagaag aaaaaatttc aagttaccaa aggaaataaa
    12841 tttaaaagta accaagtaat aacttataaa gcggctcgta tggattttgt tagtattaat
    12901 ttccgcttaa aaaattatga aaacgaatca tttcaattcc ttataactaa agcaccaata
    12961 gaaaagagac attttgaaat ttttatacac ggacaattgg gacatccagc acccgaagaa
    13021 acatactcgg ataactcgat gaaaaaattt aaaaaataca tggaagaaga aagaatagat
    13081 gcgattaaag aaaaaatatt agaatcaatc gaatctaatg atgaatttgc tgagcaaaaa
    13141 ttcgattttc ttctattgtt atcataatag ttatatttat tttttattta aaggaaagca
    13201 aggtgtaatt aatagtggaa gaagttttaa aacaatacat agcagaaaaa ttgtttaaca
    13261 aatcgaaaaa tacgatagat acatatgtaa ggtcgattaa acaattttta acctgggtag
    13321 aagattctgg tttggaagta gcttctatta aagagaataa agatggttta acgcgactag
    13381 aaatgcaaga cttcgttaga tatttggaaa aacaaaagaa aaagtctgct cagacagtaa
    13441 acaaagattg ggcagctctt gttagtttta tggaatggaa gggtcttaaa gaactgacag
    13501 aggatattag tctgccgcaa tttgaaaaat atacaaagac agcgccggat tttatagaag
    13561 atactattat taacaaaatc aaacgagaag agtatcgaaa agcacttcga acaggaagtt
    13621 ggcgagatgc agccattata aacattttga tttattttgg aatccgaatt gaagaactgg
    13681 taacaatcga tttgtccagt gttgaactaa gcgacagaaa aggtaatctg catattgttg
    13741 gaaaacgaaa caagcataga gatattcctt tcccgaaagc agaagataac aggtctttgc
    13801 gagaaattat ttctaactat ttgagcgaac gattacaaat gaagacggac gaagaagcgt
    13861 tatttttgag taatcgaggt aatcgattaa gtaagagagc ggcgcaattg atagtggaaa
    13921 aacataatgt aaattgccat gcctttagac atacttacat tactaaattg gtacgtcagg
    13981 gagtagatat tccgttaatc atgagtttaa caggacatag ttctgctgaa atgatagctc
    14041 gatacagcaa accaaacgaa gaaaatagga ttgatgctca aggaaaggta agtttcgata
    14101 gataggagaa ttataaatgt tccattctaa cacaccagat tccgatgatt tttcgatgat
    14161 tgagaaattg tttgctaagt acaaatttaa ttctctgtat gaacaagtaa gattacggct
    14221 acttgttgtt aaggactcat ttcgtgataa ggatgaattt gaaaatatag tagaggaagt
    14281 tttacttatt agtgaccaag aaggtagaaa actagatgtt aattctttct acttttcgaa
    14341 agagtgcaag aagacttaaa aaaggatgga gaacttgaat ttcaggggga gaggtagttt
    14401 atttcttgat gtgatagttt tttttatgta tagttacggt agaaaaggtt gatttatttg
    14461 gggttgaaca gtaacatatg atgtttttaa attagcgttt tgttgtatga gtataaacat
    14521 atgttggagt tgaagaataa cataggatgt ttttaaatta cataacaata gtaatagttc
    14581 gccactatac atgttgaaca acaacatagg atgtttttaa atctgtgctt cacttcgtaa
    14641 ctgtgcgtca gggttagttg aacaataaca taggatgttt ttaaatttca atgcggctga
    14701 aatcttcgcc atagtcaatt ttgttgaaca ataacatagg atgtttttga ataaaaaagg
    14761 ggactttctt atcgggaagt ctcttttttc ttgttttttt ccatttttta ctcattttcg
    14821 catgtatttc ggctgaactt tctacctttt tgtcaaaaaa agaaggacgt tgaagaaaaa
    14881 aatagtatat aattaatgta ccccaaaaaa agggcgttat atttttaagg gagatgaaca
    14941 aataaatgaa aaaagtaggc agacctgaag ttccagagaa gaaatatcga cagaaaaaaa
    15001 tacgattttc gaaatttgag gactttataa caacaatagt cgaagaaatc gatgatgaaa
    15061 aatctaaaaa taaaattcgc gatttgttta ttgcggaaag agataaagtt atatctcgaa
    15121 cagtaattta gataggaaat aacaaaatgg atgaaatttc atcaaaaaat acaaataaaa
    15181 agtatcattt ggcaacaatt aatgtattat tggttctaga aaagaaaaaa accagagcct
    15241 cggaaactct ggtcttttag gagaagatac tcatatcaac tcactcaaac ggattgcacc
    15301 ccgttattaa attcaattat ctaaagtaat aatatcatat ttttaaaagg attcaattaa
    15361 attctattat attttttcta gtatttaaaa caaatccttt gaaaaaatga cattacttta
    15421 aaataacgag ttgaatgagt accttctcca gaaaattcat tttttaatgg agaggttttt
    15481 taatatgaaa aaaatagaag aaaaattagt tttacaagat gttcaaattt taaataatgc
    15541 aagaaagcaa attgaatttt taaagttaat atatccagat gctacttttg aaaatggata
    15601 caaagacgga acgatagaaa ttagattttt aaaacgaaca agcgaaggga caagaccttt
    15661 aaaaagtttt aacacatgga ctttgtttga aacagatatt gaatatttac aaaaaacatt
    15721 gtccgtttac aatggtttgc catgttgcac ttactacagt tcttatactt ttaatcgtat
    15781 gaataaaaca ggtgaaaaaa gtattagtat caacaatgaa aatgctctac atacaagtat
    15841 ccttgcaatg gattttgatg gaattggtga agtggatttt cttgttcaaa agaaacgatt
    15901 actagatata gggattgaaa cggtggatgt atttacaggt cacgggtatc aatcaatcat
    15961 tcttttgaaa gaaaaaagtt atgataaaga gttattaaaa aagtttacgg aattattatt
    16021 aagaaaaagc tttcctgttg attcacaaat caaagacgct gctcgtgttt tgcgtcttcc
    16081 ttatacatat aattgcaaag catttttagg aggcgacaaa tattataatc ctgttaatcc
    16141 tgaagggatt caaacctttg tttatgatac aacaacgaag agatatgatg ataagtgggt
    16201 atttgaacaa ttagaaggtt tagaagatgc accaaatgaa ttggaagaat tttttgatga
    16261 attcttccca aaatctgaag aaataatccc ggtagaagta cctgcatcga aagtttctga
    16321 tgacattatt gaagaagtaa aaaatgaaaa aaaaggtgta gtctttaaaa cagatgcact
    16381 aaaagaacaa ataaatgtcg aaattaaaac aaaaaaagaa tacgaagaat tgtacaagca
    16441 ttttaattat attcgtctac ctgaagctat ccaaaacatg cttatgggta cgcctttttc
    16501 aggaattggc aatgacactt taaaattttt agtaccattt ttaaagaatc atcttggttt
    16561 aagtcttgaa aaacaaatcg aaaccttgca aacttgggga gaactttgta agccggtttg
    16621 ggataaagaa gaaatcaaaa aagaggttaa gcgtcttaat aaatacgaaa tcaaagctca
    16681 atatggtgaa tatactgaga acatgcaaaa agtatatggt gatttagttt ttgataaatg
    16741 ggtaaatgat gatgaaatca tcataatgaa tgaaatcttc gaaaaaatta aagacatcac
    16801 cgacggtgca tttcggctat acttatcaat aaaattactt gttcatgaag aaaaacgcag
    16861 taattttacc aatgaagaaa tttgcgaacg tgctgaaatc gcaattcgta cttttgaaaa
    16921 gcatattgga catttaagaa agcaaagatt ggtaggtaga aagatttcaa atcgttccaa
    16981 aggcgataaa tatgaatatt acctaaatcc atatgaaaat acagttaaag gttttacaaa
    17041 aattaaggca agcttgatag aattattatt tttaaaaggt ttaaatgatg ctcaaataac
    17101 tttctatctt tatcttaaac acatggttgg tgtatctgaa gaaagttgct gggcttctca
    17161 aacatacata gctaaagaat tgggtaaaaa aagtcaaaca accatttcta aaatgacaga
    17221 tgaattacat aaaaaaaaga taatttataa agagacaaaa aaagtaggaa aagttgttca
    17281 ttcaacatat ttattgttga agtaagaagg tcgaaatcga ccttcttttt ttatttcagg
    17341 agtaaaacat tgatgtagca agggttcatg ccccttaacc gcacgatttt catacgtaaa
    17401 tcttaagctt aaccgcacga ttttcatata taaaaaagct tgtttttcaa gggttcatgc
    17461 cccttaaccg cacgattttc atactaacaa taaaacaggg ggggagggga gaaggagaga
    17521 gaactatgtt ttatgtgttt taaagatgga ttagaactta tggatattta tacaatatca
    17581 gtaatacaac ttactatttg taattaactt agagatagat tacataatga taacactatc
    17641 tatttaggga tatggggttt atggtttcgt ggtttgaaaa caaaatatag aataataaat
    17701 aaaatatgga tagtggttat gaattagttt aggaggaagt ttaccccatg tgtcccttta
    17761 aaagacgaat ttatatagta gaagtgattt ataaaaaatt aaactgatat gtccctttag
    17821 aaagaatcat atagtaagta gagtgttaag agttttctct ggaatagaga tggttctttt
    17881 tattgcggta cttgaaatta ttaaagataa attaatcctc tgcataatga aaaattctgt
    17941 ataaagtatt gtcatattta aaaacatctt tcatagaata gtaaaaacta aatagaactt
    18001 ggcgcaaaat aaaaaatcca attgcagcca tcctattgtt tttcaagaag gaatggctgc
    18061 aattgtaacg tagccattcc ttcttgtttt tttgtaagta aaaaataagg aggaatacaa
    18121 aaatggacaa aaataatgta gagttttcgg agtttgggca tttaattgca agagaggcat
    18181 attacaaaaa tttttatgac aaagggctag gagaaccact ctgccaaaca attcgcggat
    18241 atgcaccaga tgctagtgaa cttgaaaaat ttcttgattg caagcttggg gtcgatatga
    18301 tttttgaagt ggattcaata attacaactc gcttgggaac atcatatatt ggtaaacgta
    18361 aacgtacagc tcaagaacgg tttgtccgaa atatgcgaga ttcaattaca atttctattc
    18421 aaacaggttc aggacatgaa ggagagcttt tccgattgga ccttgctagt cactttattt
    18481 atggagaata caatggaaca gacatcacag acatgatttt tatcacaaat acaacacttt
    18541 tagctgatgc gatttttgat aagaaaattc tttttacgac tcatgaaaat ccaaacaacg
    18601 gtacaatctt cattgcaatt aaaatcgaag aactagaccg aaacaatatt ccttacgttc
    18661 gatttaaaaa caaaaacaaa ttgcattaaa caaaagaaga aggaattccc ttcttctttt
    18721 gtttttatag taattaatat atgatatgcc tttttatatt agacttgcat gcttaacatg
    18781 tgggttcttt ttaattataa cgggtttaac ggctccattt agacgatttt aaaatatata
    18841 tagtgttatt tatcgttttg acaaaaaaac gcgtttaacg gcaaatttag aaccgcttcc
    18901 ctatcattct agttttttgt tttagggaag catgtcgatt ccaaagaaag acttataaag
    18961 gaaattcggt cccgaaaatt tcaatttaat atatttataa attgaaattg aataatatag
    19021 aatttaataa taaaaaaagt gcggctgaag ccgtgacccg ttcctcgaca aatgtagtct
    19081 acagttcgac gaatcttagg gaaacttatt taaatgtgtc aaaagaaaaa agccattcca
    19141 tatgttcgtg aaatgggatt cccttgatat tagaggttgt tctgtacgga aactgttttt
    19201 tcttctggga ctcctaaact tcgcttccac aattgaatag aatcttgcag cgcttgttct
    19261 attattcttg ggtggtggga ttgttgacat tagcttaaaa ttaacgtatt caatttcatc
    19321 tttatttaat atagaattga attcgtcgtt tttattaaag gacgacgagt gacggatgtt
    19381 gaaataataa gaagtataaa tgacgacgag tgacggtagc tttgatgacg atgatttagg
    19441 aggtgaaatt ttaaatggaa cttttaagaa aaaaagattt agaaaaacga atcgggaaac
    19501 cgaggaccac aataaatggt tggataaacg attataacat gtacatacca caaacggagg
    19561 atggtaaaac tacatatttt actaaagaat cagtggaagt tttgctgaaa atcaaagaac
    19621 tgaaagaaaa aggtctcgaa aaaccactaa tctttgaaga attgcaacga gaaggattta
    19681 caattcaggc aacagaaatt ttagaggttc cggtttcaaa agaagtgttc ttttcagaag
    19741 atgataattt tcaaatcttc atgaaaaagc tatttgaaaa cacagcctcc aaagaagaaa
    19801 tgcaagtgct gaataaatta ctgaataaaa ttgacggacg aatgacggat attgaattgg
    19861 aaggctctaa acgtgaccga cgaatgacgg atatgaaaaa gacgagcatc cagtacatga
    19921 aaaaagtaaa tgaattagaa cgtaatttca gtgaaataga agaaagaatt aaagatgcaa
    19981 ataactcaga tgagaaatac tacgagctag aaaaagagaa tgctattttg aaaacacagc
    20041 tggaaaattt tgagaagaaa ttaaaagatg tggaagatag acagtacaaa caactagaaa
    20101 agaaatcgtt ctttcagcgg ttgtttggaa aataaaagag accatagcgc attatggtct
    20161 ctttttgttt tttaatagaa tctcactatc aatctttctg aaataaacga aagccccctc
    20221 ccctcttcta ttaactgtaa ctaatggaga tgttttgata gtaatattta gtattattta
    20281 ataatataat actcgttatc tataatattt attcttgtaa tcttcgataa taatatagaa
    20341 ttctagtatc ttttcgggag ttataaggat aatacaggcg gtcgaacaaa tatgtaatac
    20401 aaaatattat taaatattat tttgtattac ataataatat ttatcttgat tcgtccttag
    20461 gaataatggt aaaatatact tatacttact taattactta cttaactact taatttaaag
    20521 ggagagattt tttatgacaa agacttatgt attaagtgtt gccaatgaca atggtaattc
    20581 tgaacacaaa ttaaccgtga atggaacaaa agtgagacaa ccaaacgtgt atgctcgaat
    20641 ttcaggtccg aaattcgaaa agaataatac cttagaacaa gctattcagg aattacacgg
    20701 aaatatcgat gtaacaatca gtagtccagc aattgcggat gctgataata ctagattcct
    20761 aattggacat gctgcaatga tgggtgtggg agcatctaaa gttaaaaata tgaatctaac
    20821 aaaaggtaag aagcatgaag aagacttacc aatcattaat acgctaggaa tcattgctac
    20881 taaaactgtt caaaaacatt ttgaggaaac aaaagaagtt aaaaataatg atatgttaga
    20941 ggttacggtg aacatgtcga cttgtttgcc agcttctgtg tataaagatg ccacagcaaa
    21001 gaaatttaaa gaacgattcc aacaaaatac ccatctagtt actgttaaca ttaagagtga
    21061 tttaaaagta atcgtgaaaa ttaattttgg ctatgtagga gtgacggcag aagggacttc
    21121 accactattt gaaatcatag aggatggaca aggaaactat cgcacagggg aaatgttcaa
    21181 agaattctcg gaagaatata aaatggagga agttgatggt tcttattttg cggacaagaa
    21241 cttaatgcat gttgatattg gtgatggaac gacagaatta attttgacgc acggctacga
    21301 tgtcaaacat agtagtggtt tgaaatatgg tttaggtgta gcgttagaag aagtttcgaa
    21361 gaaatgggaa gataaaaaat cctatacgag acaggatatc agtgacattg caaaatcaga
    21421 atctcatgca ttccatagtg atattataga agatttaaat ggtgcgccgc tgcaaaactt
    21481 ggcgtctgat atttcgcatg atgttaaaca attccttgat gaattacgag catatcaaat
    21541 tcaggttatg gttgtgtatg gtgggggttc gattttattg aaaaatcacc tgtataataa
    21601 gttaaaagaa attgctgagg aagagagatt ccaactgttg tggattccat cgaagttctc
    21661 tgtggacatg aacgtaaatg gtttagatac ttttaacaag atttatatgg aagaactaat
    21721 tgctgaggct gctgctacag ctgaagaaga ataaaatgga tgataggggg aattgttcgt
    21781 tgattcctcc tatatattaa aatttcagga aaggagatag tgattatgac tgaagcgaag
    21841 aaaccaacta tacaaattaa aactagtagt ccaggttgtc cagatatggt aacgaaatgg
    21901 tcactagaac agcaaaacgt aaacgattct gtaaaagaat taatttctat cattgccact
    21961 tatacaggtt atgtagaatt attggacccg gaaactttaa gaaaaattca cgctgcattt
    22021 aataatggaa caacaagcgg gatttctaat gatgcttcaa cgattgaaaa acaaatacat
    22081 agtgaaacaa aaactgtaga gggaaaaaaa caagaagaaa acggaactaa ggataataaa
    22141 aaagttaaaa aggaagaacc taaacaacag aataccgatg aaacgaataa agtgaaacct
    22201 gttttgaaca gaaaaagagg aactaacttt atgaaataaa aaataccacg ccaataggag
    22261 tggtattttt tattacttaa tcacttaatt tacttgttta aaggttctat gaacattata
    22321 aataatttat aggattcctt caatcaaaaa catcgaaaac aatcaaaatc ttgaattatt
    22381 aacaaacgag attataatac tctaagtttg ggaggtggaa acttggggaa ttataaggtg
    22441 ttgtcaaaag aagaaatgag tgaacaattt acaaatgatg atgaactaat tcaatatatt
    22501 ttatctggag aattatccaa aaagcctttt gttgttgaaa aaagagagtt acaagaaaca
    22561 gaaaataaaa gagctgcgga agagggaagg gaaccggtac atatttcgga tttaccagat
    22621 gtgcaaataa cagacttcat cgttacgaaa ggttttgatt ttgaaaataa agatgatggg
    22681 aatttaattt atccatacta taacgaaaga gaagactctt tttatgattt gttgttccag
    22741 ggattgaaga tgttacctgc gaagggtttt ttctggctag tggatactac ggtagagaat
    22801 aaaccatttg gtatgtatcg taccgaaaca agtatatcta aggtaatgag aaaacaaaat
    22861 gattttcggt ttcccaatgt acaaaaattg agagacaatc ctagagaggg gaagtcgtat
    22921 attgatgaaa aatactttta ttatgatttc gaagaggaaa aatatattca ggtaaagata
    22981 gaattagata aagaaggaaa
//
