LOCUS       NZ_AP022885             3163 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0060 plasmid pMRY14-0060_8, complete
            sequence.
ACCESSION   NZ_AP022885
VERSION     NZ_AP022885.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203324
            Sequence Read Archive: DRR206364, DRR206388
            Assembly: GCF_021654915.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 332x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021654915.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:10:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,948
            CDSs (total)                      :: 5,800
            Genes (coding)                    :: 5,583
            CDSs (with protein)               :: 5,583
            Genes (RNA)                       :: 148
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 120 of 217
            Pseudo Genes (incomplete)         :: 108 of 217
            Pseudo Genes (internal stop)      :: 46 of 217
            Pseudo Genes (multiple problems)  :: 47 of 217
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022885.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0060"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0060_8"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            114..347
                     /locus_tag="L6481_RS29575"
                     /old_locus_tag="BCM0060_p81"
     CDS             114..347
                     /locus_tag="L6481_RS29575"
                     /old_locus_tag="BCM0060_p81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="WP_046199669.1"
                     /translation="MTKLEELEKDFNQMNLDLKAIQHDMKSLEVRILVAEKDVLTINKQ
                     LDKISANTTWILRLIISGLLTGVLGVVAKNLL"
                     /name="hemolysin XhlA family protein"
     gene            715..1959
                     /gene="mobV"
                     /locus_tag="L6481_RS29580"
                     /old_locus_tag="BCM0060_p82"
     CDS             715..1959
                     /gene="mobV"
                     /locus_tag="L6481_RS29580"
                     /old_locus_tag="BCM0060_p82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562244.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000951644.1"
                     /translation="MAHVQKYAKGNVQGLSIHWDRKTENHSNQDIDNERSHLNYDLCEK
                     EGDTLSRMNDRLSEVHCLNRKDVKVCADWAVTLPESLKDASEKEQREFFEKTYEFLANR
                     YGGEKNVLSANVHNDETRPHMHFAFMPVVWDEKKQQAKVSAKEVLTRKDLKTFHQDLDE
                     FLKKEIPQIYKEGILNDKTIGVDTVKDLKRYSEEIQKQKDVMDAEVKVKERELEGKIQS
                     LDKELESKKNEFLNLSEALPSKINIKVKGKEKKTEVVKTGFLKSETVTTETGNWIVSDS
                     EMRRMQKVLSDANFVKEDYERLQRTDLVKENKELHDRVDSLADGYVKAINENTDLYEKN
                     RELRKEISSLKAHVKDLKENVKVLYHNTKKVLGEHFKAFRGLVKNELDIKGVDNQFDCE
                     YKKEIKKQRGYNMER"
                     /name="mobV"
     gene            complement(1973..2471)
                     /locus_tag="L6481_RS29585"
                     /old_locus_tag="BCM0060_p83"
                     /pseudo=""
     CDS             complement(1973..2471)
                     /locus_tag="L6481_RS29585"
                     /old_locus_tag="BCM0060_p83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182216.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /name="replication/maintenance protein RepL"
     Typing          716..1956
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagagaactt aaatcttaat aatagatttg agttctcttt ttatatcatt attctctaat
       61 taccatatac atataatata tttaaaatta atttaaaagg agaagtatgt tatatgacaa
      121 aattggagga actagaaaaa gattttaatc aaatgaatct agatttaaaa gctatacaac
      181 atgatatgaa aagtttagaa gtaagaatat tagtagctga aaaggatgtt ttaactatta
      241 ataaacaatt agataaaatt agtgcaaata ctacatggat tttacgattg atcataagcg
      301 gattgttaac aggagtactt ggtgtagtag ctaaaaacct gttataggaa aaaagggtgt
      361 tggtcaatca aaacggacct agcaaagcga agggagtatt tgacttgacc aaccgtacta
      421 cacccaaacc ctcttggcat tgaacaaaca atcgtttgtt cctgctaacc ggcgagggag
      481 cccctcaagg attgaggggt tggggagttt gattgagggg gtctggggaa gagtttcccc
      541 agtgggtttg ggcaaagccc aaggtttgtt ttttgccagg caaagcatgg cttggcggag
      601 ccaaagagcc tcgcagagca cgtccaaatg aaatttggta taacgtgcta gaccaaattt
      661 ggtttttctt ctatccccat gttaaactag ttaccaaaag ggggggtctt gctcatggct
      721 cacgttcaaa aatatgcaaa aggaaatgtt caaggattgt ccatccactg ggacagaaaa
      781 acagaaaatc attcgaatca agacattgat aatgaacggt cacatttgaa ttatgattta
      841 tgtgaaaaag aaggagatac actttcacgc atgaatgatc ggttaagtga agtgcattgc
      901 ttgaatcgaa aagatgtaaa agtctgtgca gattgggctg ttacattacc ggaaagtttg
      961 aaagatgctt ctgagaagga acaacgtgaa ttttttgaga aaacctatga atttttagcg
     1021 aaccgttatg gtggtgaaaa aaatgtgttg tcagccaatg tacataatga cgaaacaaga
     1081 ccgcatatgc attttgcttt tatgcctgtt gtttgggacg agaaaaagca acaggcaaag
     1141 gtttcagcta aagaagtttt aacaagaaaa gatttaaaaa catttcacca agatttagat
     1201 gagtttttga aaaaagaaat accacaaatc tacaaagaag gtattttaaa cgataaaacg
     1261 attggcgttg atacggttaa agatttgaaa aggtattctg aagaaataca gaaacaaaaa
     1321 gatgtaatgg atgcggaagt aaaggtcaaa gaaagagagt tagaggggaa aattcaatca
     1381 ttagataaag aattggaaag taaaaaaaat gagtttctga atttgagtga agcactccct
     1441 tcaaaaatta atatcaaagt aaaaggaaaa gagaaaaaaa cagaggttgt aaaaacaggt
     1501 tttttaaaat cagaaacagt aactacagaa actgggaatt ggattgtttc tgatagtgaa
     1561 atgagaagga tgcaaaaagt tctaagcgat gcaaatttcg taaaggaaga ttatgagcgt
     1621 ttgcagcgta cagatttagt taaagaaaac aaagaattac atgatagagt tgatagttta
     1681 gccgatggtt atgttaaagc tattaatgag aatacagatt tgtatgagaa gaatagagaa
     1741 ttacgtaagg aaataagctc gttaaaagcc catgtaaaag atttaaagga aaatgtaaag
     1801 gttttatacc acaatacgaa aaaagtcctt ggagagcatt ttaaagcgtt tagaggactt
     1861 gttaagaatg aattggatat aaaaggtgta gataatcaat ttgattgtga atataagaaa
     1921 gaaattaaga aacaacgtgg atataacatg gaacgttaaa accgtctcag aattaattct
     1981 gagacggttc gtcgtttttg tatgttattt ctgttttaag ctctcttcct ttagttgaat
     2041 agacttgttg taatcggatt tcttgtatat cgtgccaatg gccacgacca aaataacgag
     2101 catttacttg atatgtacca gtaccttttc taaaaagaat ttctgcattt acaagtttgt
     2161 tgatgttgtt attaagaact tgtactgttt tatatcctag ttcttgtgcc atttgttttt
     2221 ttataaagct atttagaatg atcatattgt cataagacat tacttttaat aaaacgttaa
     2281 taactctatc taaaccttta ggtaaatcat ttaaggctac aatatctttt aaatatattt
     2341 ttacgtagtc tggttctttg tcgatataag catttataat ttcttcttct tcttgaattt
     2401 caccagtatt tatatctatt attttatttt tgtaacttta gaaacttttt caagggtctt
     2461 tccggttgca tcaaatctac ctcctacccg atttataatt taattaaatt ataaatcggg
     2521 tattttaaaa gtgcaatatt ttttatagga tttcacattt ataatcccac aaattataaa
     2581 tgatataaaa ataaatttaa tttataactc cacaaattac ggtatataac tccacaaatt
     2641 acggttataa tcccacaaat tataacttat aatcccacaa attatagtat tttcctagaa
     2701 atccagtcat atcaacggtt caacctatgt tctcccttct attctattat tttataaaat
     2761 ttaaaagggc ttttcactat gcaatagtcg agtagtattt tttgagaaaa ccattcaaaa
     2821 aatctccact cttttgctat tgcatagtga aattcggcag gtgtgagcat gtcgccaagc
     2881 cgaacagacc aaaaacgagg atttagattg ttcgacttgg actagcaatt gtacctgccg
     2941 aaccgccttg ctctatcgtt ctaaacaatc cgcttattcc atattcattt acgttaagat
     3001 ccagggggct agcccccaaa cccccagcca gcactcacac ccaaaaacaa ggagagaatg
     3061 aacttagaac agcacattta tagaaactgt gtttttgcta gatgcatatt aaaaaggata
     3121 agcatatgct atattacgct ttcgtataat tcccttatct tta
//
