LOCUS       NZ_AP022884             4021 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0060 plasmid pMRY14-0060_7, complete
            sequence.
ACCESSION   NZ_AP022884
VERSION     NZ_AP022884.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203324
            Sequence Read Archive: DRR206364, DRR206388
            Assembly: GCF_021654915.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 332x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021654915.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:10:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,948
            CDSs (total)                      :: 5,800
            Genes (coding)                    :: 5,583
            CDSs (with protein)               :: 5,583
            Genes (RNA)                       :: 148
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 120 of 217
            Pseudo Genes (incomplete)         :: 108 of 217
            Pseudo Genes (internal stop)      :: 46 of 217
            Pseudo Genes (multiple problems)  :: 47 of 217
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022884.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0060"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0060_7"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            join(3652..4021,1..365)
                     /gene="erm(C)"
                     /locus_tag="L6481_RS29560"
     CDS             join(3652..4021,1..365)
                     /gene="erm(C)"
                     /locus_tag="L6481_RS29560"
                     /EC_number="2.1.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /protein_id="WP_236807551.1"
                     /translation="MNKKNIKDSQNFITSKRNVDKIMTNISLNEHDNIFEIGSGKGHFT
                     LELVQRCNFVTAIEIDHKLCKTTENKLVNHDNFQVLNKDILQFKFPKNQSYKIFGNIPY
                     NISTDIIRKVVFESIADESYLIVEYEFAKRLLNTKRSLALLLMAEVDISILSMVPREYF
                     HPKPKVNSSLIRLNRKKSRISYKDKQKYNYFVMKWVNKEYKKIFTKNQFDKSLKHAGID
                     DLNNISFEQFLSLFNSYKLFNK"
                     /name="erm(C)"
     gene            complement(438..1421)
                     /locus_tag="L6481_RS29565"
                     /old_locus_tag="BCM0060_p71"
     CDS             complement(438..1421)
                     /locus_tag="L6481_RS29565"
                     /old_locus_tag="BCM0060_p71"
                     /function="involved in plasmid replication"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_014125933.1"
                     /translation="MCNNNSINSDEKSSVLIDKTKSGKIRPWREKKMANVDYFELLHIL
                     EFKKAERVKDCAEVLEYKVSYETGEKTLYRVWFCKSRLCPMCNWRRAMKHGIQSQKVVA
                     EVIKQKPTVRWLFLTLTVRNVYDGTELNKSLSDMSKGFNRMMKYKKINKNLVGFMRATE
                     VTVNNIDNSYNQHMHVLVCVESTYFYNTENYVNQKQWIQFWKRAMKLDYDPNVKVQMIR
                     PKNKHKSDIKSAIDETAKYPVKDTDFMTDDEERNLQRLADLEEGLYRKRLISYGGLLKE
                     IHKELNLDDAEDGDLIHTDDEEKADEDGFSIIAMWNWERKNYFIKE"
                     /name="protein rep"
     gene            complement(1665..2927)
                     /gene="mobV"
                     /locus_tag="L6481_RS29570"
                     /old_locus_tag="BCM0060_p72"
     CDS             complement(1665..2927)
                     /gene="mobV"
                     /locus_tag="L6481_RS29570"
                     /old_locus_tag="BCM0060_p72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_236794100.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYD
                     LKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFE
                     ESYKLFSERYGKQNIAYTTVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKF
                     PEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVK
                     SDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDND
                     RLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK
                     ISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE
                     FELTHNREVKKERSRDQGMSL"
                     /name="mobV"
     ARG             4..365
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="95.0"
                     /coverage="49.18"
     Typing          complement(439..1421)
                     /name="rep_cluster_2104"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1669..2927)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.952"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atttaatcgt ggaatacgag tttgctaaaa ggttattgaa tacaaagcgc tcattggcgt
       61 tacttttaat ggcagaagtt gatatttcta tattaagtat ggttccaaga gaatattttc
      121 atcctaaacc taaagtgaat agctcgctta tcagattaaa tagaaaaaaa tcaagaatat
      181 cgtacaaaga taaacagaag tataattatt tcgttatgaa atgggttaac aaagaataca
      241 agaaaatatt tacaaagaat caatttgaca aatccttaaa acatgcggga attgacgatt
      301 taaacaatat tagctttgag caattcttgt ctcttttcaa tagctataaa ttatttaata
      361 agtaagttaa ggggtgcgta aactgcaccc cttaacttgt cttgttttgc ttaaacaatc
      421 ttggcccttt tgttgaatta ctctttaata aaataatttt tccgttccca attccacatt
      481 gcaataatag aaaagccatc ttcatctgct ttttcttcat catctgtatg tattaaatcg
      541 ccatcttctg cgtcatcaag atttaattcc ttatgaattt cctttaataa accaccatat
      601 gaaattaatc tttttcgata aagtccttcc tccaaatcag ctaaacgctg caaatttctt
      661 tcttcgtcat cagtcataaa atctgtatct tttactggat attttgcagt ttcatcaatg
      721 gctgatttta tatcagattt atgtttattt tttggtcgaa tcatttggac tttaacgttt
      781 ggatcataat ctaatttcat agctcttttc caaaattgaa tccattgctt ttgattcaca
      841 tagttttcag tgttataaaa ataagtgctt tctacacaaa ccaacacatg catgtgttgg
      901 ttataagaat tatctatatt atttacagtt acttctgtcg ctcgcataaa accaactaag
      961 tttttattaa ttttcttata tttcatcata cgattaaaac cttttgacat atccgataaa
     1021 cttttattta attcagtacc atcataaaca tttctaacgg tcaacgtaag gaaaagccaa
     1081 cgaactgttg gtttttgttt gataacttct gcaacaacct tttgtgactg aataccatgc
     1141 ttcattgctc tcctccagtt gcacattgga cataatcgag atttacaaaa ccacacacga
     1201 tataacgttt tttctcccgt ttcataactc actttatatt caagtacctc tgcacaatct
     1261 tttacacgtt cagccttttt aaattcaaga atatgcaaga gttcaaaata atctacattt
     1321 gccattttct tttctcgcca aggtcttatt tttccacttt ttgttttgtc gattagtaca
     1381 cttgattttt catctgaatt aatgctatta ttattgcaca taattaatac agaaaccccc
     1441 tatgtaaatt tgtttttgtg tggtaactta aacaataaca tagggtattt ttctgtgcaa
     1501 ggatttttaa aggttcatta accttataaa gccaatcaca ccaacacttt aaacgcgatg
     1561 taaactataa aaataaaaac ttgttgtttc tatatgtatc aagataagaa agaacaagtt
     1621 caaaaccatc aaaaaaagac accttttcag gtgctttttt tattttataa actcattccc
     1681 tgatctcgac ttcgttcttt ttttacctct cggttatgag ttagttcaaa ttcgttcttt
     1741 ttaggttcta aatcgtgttt ttcttggaat tgtgctgttt tatcctttac cttgtctaca
     1801 aaccccttaa aaacgttttt aaaggctttt aagccgtctg tacgttcctt aaggaattcc
     1861 ttagtgcttt catagattaa actcacatca cgctttaaat cgcttatttt agactttaaa
     1921 gacttgtttt cttcaagcaa ctcattataa tcatttacat tttcattaaa tcgctctaca
     1981 agaccactat atttttcttt aacttgccca tgttctttac ttaatttttt atattctctc
     2041 gccatatcag tactcatgag atttctaaca tgctgtttta acctatcgtt atctctcgca
     2101 gcagtcacta agtttttata atcacgctcc gatataacaa catttttggt tggtttcttt
     2161 tctgttttca ttatttcttt tcccaaacca aacatggact tttcacccgt tggcacttca
     2221 acacttttca tgtgtcgttt cgctggtact tctaaatctg atttaacttt atcgctataa
     2281 gcagtccatt catctttttt aactgctaaa tttttttcta gaaaatcaat ctctttttcc
     2341 aaagtttgtt ttttaaattt agctgtctca atatgtttac ggtcagagcc acgttcacca
     2401 cgcttcaact caaaaccctg ttttttcata tgctcgggga atttatcttg tagccataac
     2461 agttcttgac gattaaacac attttttcct tgcagttttc catcacgcat aggcacaaca
     2521 cctaaatgca tgtgaggggt ttgctcatca ttatgaactg ttgtataagc aatattttgc
     2581 ttgccatatc gttcggaaaa taatttataa ctttcctcaa aaaatcgttt ttgttctcct
     2641 ggatccagtt gctcaaaaaa atctcggtca gatgttacta gcaactcatt tacaagaaca
     2701 gcatctttcc tcgtttttct tgtacctgtt ttttgtgatt caataatttc tttgacacgt
     2761 tcgttgtaat caatattttt atcatttttc aaatcataat tttcacgtgt tcgctcatgg
     2821 tcaatatcat cattcgttct actttttcgc tctctttgat tatgaaattg catgcccttt
     2881 agtccagctg atttcacttt ttgcattcta caaactgcat aactcatatg taaatcgctc
     2941 ctttttaggt ggcacaaatg tgaggcattt ttgctctttt ccggcaatta cttccatgta
     3001 aagtataaca cactatactt tatatccata aagtgtgtgc tctgcgaggc tattggctct
     3061 gccaacaaaa ccctgcatac tgattaccag tatgcacaaa acctttaaga cctttctttt
     3121 ttttacgaga aaaaagaaac aaaaaaacct gacctctgcc acctcagcaa aagggggatt
     3181 tgctctcgtg gctcgtttaa aaaagacaag ggacaggtcg tatttttcaa aacgctgcgc
     3241 tgaaaaatct ccaccttaaa cccttgtctt ttttattttg tccgttatgt ttttcgtacc
     3301 gaaagccgta ctcagcaaaa tcaaaatttg ctcgactttc ggttttccag gataacggac
     3361 aaaaccactt aaaataacaa aagacacgag aatgatctct cgtatctttt gtattcttat
     3421 tcaacaatcg cgcccgattg cagtataaat ttaacggtcg ttcatcatgt tcttatttat
     3481 cagagcttgt gctataatta ttttaagttt ataagaagga gaattaatat atgggcattt
     3541 tttgtttggt tgataatcaa cacagttgat tatcaaccaa acaaaaaata agtggttata
     3601 atgaatcgtt gataagcaaa attcattata accgcattaa agagggttat aatgaacaaa
     3661 aaaaatataa aagacagtca aaactttatt acttcgaaac gtaatgtaga taaaataatg
     3721 acaaatataa gcttaaatga acatgataat atctttgaaa ttggctcagg aaaagggcat
     3781 tttacccttg aattagtaca aaggtgtaat ttcgtaactg ctattgaaat agaccataaa
     3841 ttatgcaaga ctacagaaaa taaacttgtt aatcacgata attttcaagt tttaaacaag
     3901 gatatattgc agtttaaatt tcctaaaaac caatcctata aaatatttgg taatatacct
     3961 tacaacataa gtacggatat aatacgcaaa gttgtttttg aaagtatagc tgatgagagt
     4021 t
//
