LOCUS       NZ_AP022883             4045 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0060 plasmid pMRY14-0060_6, complete
            sequence.
ACCESSION   NZ_AP022883
VERSION     NZ_AP022883.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203324
            Sequence Read Archive: DRR206364, DRR206388
            Assembly: GCF_021654915.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 332x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021654915.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:10:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,948
            CDSs (total)                      :: 5,800
            Genes (coding)                    :: 5,583
            CDSs (with protein)               :: 5,583
            Genes (RNA)                       :: 148
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 120 of 217
            Pseudo Genes (incomplete)         :: 108 of 217
            Pseudo Genes (internal stop)      :: 46 of 217
            Pseudo Genes (multiple problems)  :: 47 of 217
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022883.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0060"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0060_6"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            join(3576..4045,1..583)
                     /locus_tag="L6481_RS29535"
     CDS             join(3576..4045,1..583)
                     /locus_tag="L6481_RS29535"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071727126.1"
                     /translation="MDYKEQHRYKKKLQRYQEMMIEQETAYEKTKESYPNYVEPYDEKL
                     VISGFHIIHSQYEKPIWRGFKRNDEAVEQGAKNRKISMTIRKELDNELQNYVDRFPSAT
                     KHDKQAMKELKRIKDAYKDVLQIREDNEKRKIRTLKNKIQANFDEWTHFLTLTFKENLI
                     DITRAKEKLKEWVKKMKVLYPDFLYVYVMEFQERVAIHFHVLCRMDKGKVVPKKKFMQT
                     RKTWDKLGNIDIKGIRYKYLPKKHVNEAKTELQELSASERMVTIWSVGNYLTSYLEKGA
                     DDVLLFGSKMYGASSGLKEEIVLRDKKRIAAILLELGVERLKEKTYEIQQTETDNTITM
                     KCYNQLILKN"
                     /name="hypothetical protein"
     gene            complement(882..1484)
                     /locus_tag="L6481_RS29540"
                     /old_locus_tag="BCM0060_p61"
     CDS             complement(882..1484)
                     /locus_tag="L6481_RS29540"
                     /old_locus_tag="BCM0060_p61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562236.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_061684763.1"
                     /translation="MNIKKFGYVRVSSKDQNEARQIEAMKEQGIEERDLFIDKQSGKDF
                     SRDKYQALKSCLREGDILYIHSLDRFGRNKDEIEKEWKEITKDIKADIVVLDMPLLDTT
                     KHKDNMGTFISDLVLQILSWMAEEERKKIKQRQREGIDVALKQGVRFGRPKTEATEEFY
                     QAYGKWKAGEITATKAIEEAGMKRTTFYKLAKQIEEQ"
                     /name="recombinase family protein"
     gene            1815..2069
                     /locus_tag="L6481_RS29545"
                     /old_locus_tag="BCM0060_p62"
     CDS             1815..2069
                     /locus_tag="L6481_RS29545"
                     /old_locus_tag="BCM0060_p62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562235.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_197242842.1"
                     /translation="MIFDEKNVLVDVDGLPLYQRLRIKRVLDGFTQPQLCELLGLPNAP
                     YLSRMERGERPIPKRCMERIEQYLYKEVYKDGQLQKNVG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2041..2250
                     /locus_tag="L6481_RS29550"
                     /old_locus_tag="BCM0060_p63"
     CDS             2041..2250
                     /locus_tag="L6481_RS29550"
                     /old_locus_tag="BCM0060_p63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080448278.1"
                     /translation="MVSYKRMLVEVDTIPDTEDGRKQADFLSKLKEAVTREEQPHVPYK
                     NLDVIAERLEDDSYRCTPNKLLQS"
                     /name="hypothetical protein"
     gene            3060..3326
                     /locus_tag="L6481_RS29555"
                     /old_locus_tag="BCM0060_p64"
     CDS             3060..3326
                     /locus_tag="L6481_RS29555"
                     /old_locus_tag="BCM0060_p64"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236807549.1"
                     /translation="MTTNRQLNMGEISSIQLDRLTIVGTIRRDKENYSPLFMKLLNGID
                     KRLQVDPYYKNHLYDRKIGVRTEESTAFIELSKNKTMYASELM"
                     /name="hypothetical protein"
ORIGIN
        1 attcaaagaa aatttgattg atataacacg agctaaagag aaattgaaag aatgggtaaa
       61 gaaaatgaaa gtgttgtatc ctgatttctt gtatgtgtac gttatggagt ttcaagaacg
      121 agtagctatt cattttcatg tgttatgtag aatggataaa ggaaaagtgg taccgaaaaa
      181 gaagtttatg cagactagaa agacatggga caagctaggg aatattgata taaaagggat
      241 cagatacaaa tatctcccta aaaagcacgt gaatgaagca aaaacggagt tacaggaact
      301 ttctgcgagt gaacgcatgg ttactatatg gagtgttggt aattatctca ccagctattt
      361 ggagaagggt gcggatgatg tactcttgtt tggttccaaa atgtatgggg cttcaagtgg
      421 tttgaaagaa gaaattgttt taagagataa aaaaagaatc gctgcaatcc tattggagtt
      481 aggagtagag cgactcaagg aaaaaactta tgagatacaa caaactgaga ctgataacac
      541 tattacaatg aagtgctaca atcagttaat actcaagaac taatcccact gtttgccgac
      601 agtgaaatgt taatatgata ctgatagtat attacaacct ttatgaataa tacaataaca
      661 tgcaataact tgtcataaac ttacatggct ctgtcgctta gacagggctt ttttaattta
      721 ccttgctaat acggagaaaa acaggtgcca actgctatat tatgcatgag gtgaatacac
      781 atggaaaaat gtatgcttat catagcgtta gttggtttag tcgctgatgt agtgactatt
      841 atcagtgttg taaaagacct gttttaattg caggtctctt tttattgttc ttctatctgt
      901 tttgctagct tatagaacgt tgtacgcttc atccctgctt cttctatagc tttggtggcg
      961 gtaatttctc ctgctttcca ctttccgtat gcttggtaaa actcttctgt agcttctgtt
     1021 ttaggtctgc cgaaccgcac accttgtttt aaagctacgt ctattccttc acgttgcctt
     1081 tgcttaatct tcttacgttc ttcttctgcc atccatgaaa gtatttgtag gacaaggtct
     1141 gatatgaatg tgcccatgtt atctttatgc tttgttgtat ctagtaatgg catatccagt
     1201 acaacaatat ctgctttaat atctttagta atctctttcc actctttttc tatctcatct
     1261 ttgtttcttc caaagcgatc cagagaatgt atgtatagta tgtcgccttc acgtaggcat
     1321 gatttcaatg cttggtattt atcacggctg aagtcttttc cactttgctt atcaatgaat
     1381 aggtcacgtt cttctatgcc ttgttccttc atagcttcaa tctgtcttgc ttcgttttgg
     1441 tctttactgc tgacacggac atatccaaac ttttttatgt tcatttccct tcctcccatc
     1501 aattgttcct taattaaatt atatcacttt tgttcgtaaa actccataaa cgttaacgaa
     1561 tattcgtaaa ctattttcga acattaacga acaacagaaa cgtgataaaa ccgtgtcttt
     1621 atatctgttc gcaaaagtgt acttaaatga acaattttat tgtcaaaaag tattgcacct
     1681 tcactttcta agggttaaaa ataaattgat cttgagtaaa tgttggttat tgttctatct
     1741 gagttattgt catagagccc acttcctagg ctgggtgggc tacataatta cactccgaaa
     1801 gggtttgatg atagatgatt tttgatgaga aaaatgtact tgttgatgta gatggattac
     1861 cgctttatca acgcttacgt ataaagaggg ttcttgatgg attcacacaa cctcaactat
     1921 gtgaattgtt aggactgcca aacgctccat atttaagccg tatggaacga ggggagagac
     1981 ctatccctaa acgatgtatg gaacgtatag agcaatactt atacaaggag gtctataaag
     2041 atggtcagtt acaaaagaat gttggttgaa gttgatacta ttcccgatac agaagatgga
     2101 cgaaagcaag cggactttct ttctaagtta aaagaagcgg taacaaggga agaacaaccg
     2161 catgtgcctt acaagaactt agatgtcatt gcagagcgtc tagaagacga ttcttacaga
     2221 tgtacgccaa ataaactact ccaatcatag tgaaacgatt atgttcggaa ggcagttgtg
     2281 agaaagaggg gttaatgcgt tagcattgac ggaatgcttg ccgtgttcct ttaaacggct
     2341 ggcagtacgg caaaggtgtt ggattaacag agtctctact catcacctgg agtcagaagg
     2401 ataaatagtg gaacgaacaa cgatagtacg ccaaaggtct ccttatttca gagcgtggat
     2461 cataacggca gcagccatca atattatggg gcgtatactg ccgtaggctt acccccctgg
     2521 gtgggcatgg aaagatgaat agggcaacaa gtaacacata ccttgcgaca tatggcgagg
     2581 atattttttt atcctagtag caattttatt gcctatgtgt agatgatgac atctaaaagg
     2641 cctagtttgg cttgtaagca gacttctgac aaacgttgat ataacaacat tcttagctat
     2701 taaagtagga ggtgtgccct ggggagaggg tcaaacggca aactctgata agatagaggt
     2761 aatggcaaat gtcttcccta tggttcggca aagttcctta tgcttcccta aaacagtcct
     2821 aaaagcgtca tattacaact tttagcggag ataggagcga atccttgggc ggtgccattg
     2881 ccgtgcccta cagcttcgtt atgatgtata gaaaataaca tttgtctttt gtaatgatat
     2941 tgagttaagg tggattacaa gggacttcaa gttgacccta ttctaatagc aggtcaacgg
     3001 tctatttttt agactttcca ctacaaccat taaatctttt tgaaaggagt aactattgca
     3061 tgacaacaaa cagacaactg aatatgggtg aaatatcctc aatccaattg gacaggctta
     3121 ctatagttgg taccatacga cgtgataaag agaattacag tccgttgttt atgaaactac
     3181 tgaatggaat agataagcgt ctgcaagtag acccgtatta caagaatcat ttgtatgacc
     3241 gtaaaattgg tgtaagaaca gaggaaagta cagccttcat tgaattgagt aagaataaaa
     3301 cgatgtatgc atctgagtta atgtgaaata caaaggtggg atttaaccgc tatatctgaa
     3361 gtgcggtttc acgatgcatt ggttaaaagt gagactgtgg gggcgggggt aatagattta
     3421 atatacaact caaatcaggg gaaaaaacta ttctccctgt agagctcttg aaccttagaa
     3481 ccataagggt ttaagccacc ttaacagaaa aatcttttgc actaattttg agctaatttg
     3541 aactaatttt aaaagaaaag gaggtttgca ctgaggtgga ttacaaagaa caacatagat
     3601 acaagaaaaa gcttcaacgg tatcaagaga tgatgattga acaagaaact gcttatgaga
     3661 aaacaaaaga atcttatccg aattacgttg aaccttacga tgagaagctt gttatatctg
     3721 gctttcatat catacacagt caatacgaga aacctatatg gcgtggattt aaaagaaatg
     3781 atgaagcggt agaacaggga gctaagaatc gtaaaattag tatgactata cgtaaagagt
     3841 tggataatga gcttcagaat tacgtggatc gtttcccttc agctactaaa catgataaac
     3901 aagcaatgaa agaactgaaa agaataaaag atgcttataa agatgtctta cagattcgag
     3961 aagataacga aaagagaaag atacgtacat tgaaaaataa gatacaagct aactttgatg
     4021 aatggactca ttttctcacg ttaac
//
