LOCUS       NZ_AP022882             5289 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0060 plasmid pMRY14-0060_5, complete
            sequence.
ACCESSION   NZ_AP022882
VERSION     NZ_AP022882.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203324
            Sequence Read Archive: DRR206364, DRR206388
            Assembly: GCF_021654915.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 332x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021654915.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 07:10:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,948
            CDSs (total)                      :: 5,800
            Genes (coding)                    :: 5,583
            CDSs (with protein)               :: 5,583
            Genes (RNA)                       :: 148
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 120 of 217
            Pseudo Genes (incomplete)         :: 108 of 217
            Pseudo Genes (internal stop)      :: 46 of 217
            Pseudo Genes (multiple problems)  :: 47 of 217
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022882.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0060"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0060_5"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            complement(194..535)
                     /locus_tag="L6481_RS29500"
                     /old_locus_tag="BCM0060_p51"
     CDS             complement(194..535)
                     /locus_tag="L6481_RS29500"
                     /old_locus_tag="BCM0060_p51"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2538 family protein"
                     /protein_id="WP_080501341.1"
                     /translation="MYFENATHEQNYNDMMKFYSLAFGENPAFESAIYIAAYSQIHDKI
                     SKSKMDKNANPLAIALESDYLSNSAESLALLGQSLFNGEKVDVSSVVTRGKEIFEIFVQ
                     ACKIRGSLN"
                     /name="DUF2538 family protein"
     gene            complement(554..907)
                     /locus_tag="L6481_RS29505"
                     /old_locus_tag="BCM0060_p52"
     CDS             complement(554..907)
                     /locus_tag="L6481_RS29505"
                     /old_locus_tag="BCM0060_p52"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_076868344.1"
                     /translation="MASKNKHKIIEENRDIFLKRTSGMAHDLESVSNILVNFGLAKNRR
                     IAQRWVRENRIKAEWLSGEDDRRAGKIVKESEIFKTITTEIPALKVMFNAIEELQKEND
                     ELKKQIEQSSKQK"
                     /name="hypothetical protein"
     gene            complement(921..1076)
                     /locus_tag="L6481_RS29510"
                     /old_locus_tag="BCM0060_p53"
     CDS             complement(921..1076)
                     /locus_tag="L6481_RS29510"
                     /old_locus_tag="BCM0060_p53"
                     /inference="COORDINATES: protein motif:HMM:NF024513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3970 family protein"
                     /protein_id="WP_175556117.1"
                     /translation="MIKIRLQGLSQEMQEMIEDLKKHYDILSISDFYQNRNSQYIRCYI
                     EMRLKS"
                     /name="DUF3970 family protein"
     gene            complement(1314..1550)
                     /locus_tag="L6481_RS29515"
                     /old_locus_tag="BCM0060_p54"
     CDS             complement(1314..1550)
                     /locus_tag="L6481_RS29515"
                     /old_locus_tag="BCM0060_p54"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_076868345.1"
                     /translation="MKPNRYGRFRKEDVLKNGYEIVEYGITATEIEKQLGVKLTMKQRL
                     KHCIGFRCEKSWRGCVPVFDILDIALKNDRNQL"
                     /name="hypothetical protein"
     gene            complement(1582..1845)
                     /locus_tag="L6481_RS29520"
                     /old_locus_tag="BCM0060_p55"
     CDS             complement(1582..1845)
                     /locus_tag="L6481_RS29520"
                     /old_locus_tag="BCM0060_p55"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_220784068.1"
                     /translation="MKERYSVTQKYMVAGVDEKEKVSLYTLIEVPAIKKRMVVCIGNKV
                     DGLVVGNTYEVELDISIETKKFDTKDGVKYVDVASKFVRDIR"
                     /name="hypothetical protein"
     gene            complement(2082..4622)
                     /locus_tag="L6481_RS29525"
                     /old_locus_tag="BCM0060_p56"
     CDS             complement(2082..4622)
                     /locus_tag="L6481_RS29525"
                     /old_locus_tag="BCM0060_p56"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_220784067.1"
                     /translation="METHECGKQSVLVQDKATKEMYGFVAQCKRMDCDSCGTRIQTYVQ
                     NKIAMYTKKFDLKHFATITMGDSDIKNLLKKFGSLVDKTRELEKEYFIKRYISRKRSTR
                     EIAEKKYYEYVHSLYDQEHYYRWLYGKDLSKKEKNINYVPISNIGYQITKMITQRPARL
                     QDQIKIILEMKQGKKLRKNQLEKYYKDNVESQRKKWVGWSYADTKKEGRREEFLNDFHV
                     LLTDIAMNMYTQLYPNEEERTIYRSKIPKENTEMAYIRTIEFHNTVDAAHIHSLFNFYI
                     PIKLLKSVMGNHVYDMAEISNLASENLEEKCEEEHDFHQYVARYVSKYITKSSLEVYEK
                     LKEEGEDRVNIISSSDNISIVLEEAFETPYKGKFQLLSVVKGVLPQHYMKFSTIQEFQY
                     FILDSIADEHPNQYIDKTISCFKDYFKEARKKGLSNRDREILKDELKANENELLQNALK
                     LELERRSKEIFVPKQLKLRKTLKQGSLTTEQFEALQGITSSDLFSFLIGYAGTGKTYTL
                     STLLDYIDFEKHDVAVVSYTAQATQRVRSMLKEQNINVDNLEINTIHKLCGAKHSMIDY
                     PIFYNDWSKPLPYSFVIIDEFSLIDIRVLTSFLASLKPTTKVLFVGDPKQLNPIHSKHP
                     YFYLKDFAGVYELTKVLRSGDRIIDVATSVRNGDIEKVPFEVMDFKNSEEKIAEYKKRG
                     YQVLSNSVALKDWINNLFQKDKKQIQSKFNYAQFDNIIFIKNDQYKRYVNGEKGQIIDY
                     NEFGVTIRTENNNEVVLKDYEFFNRVEPNHCLTVHKSQGSEYDKVLVLLDATKTLLLNR
                     NLLYTAITRAKTDVVVMINKVDDKVKTLLKQEQE"
                     /name="AAA family ATPase"
     gene            complement(4798..4989)
                     /locus_tag="L6481_RS29530"
                     /old_locus_tag="BCM0060_p57"
     CDS             complement(4798..4989)
                     /locus_tag="L6481_RS29530"
                     /old_locus_tag="BCM0060_p57"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_076868343.1"
                     /translation="MQKAKRGRPRMNKTYRQNVVRFSYMEDTIKNIMDLVDSEERDKIN
                     ALLSNLVDHEKQKVAMKS"
                     /name="hypothetical protein"
ORIGIN
        1 ccttgctgac tcgtcagcaa accacgaacc agtacctcta cccaaaaagt ctcgaaaacc
       61 cttcttctat gtattttaac aaagttcaat gttaaacttt agacttttta attataccaa
      121 ttccctcaaa gttcaacctt atacattgta tatattatat tttagtaaaa aacacttagt
      181 ttattactaa gtgttaattc aaactaccac ggattttaca agcttgaaca aaaatttcaa
      241 agatttcttt acctcttgta acaacagaag aaacatctac tttttcccca ttgaaaagcg
      301 attgccccaa taatgccaat gattctgctg aatttgataa gtaatcagat tccaatgcaa
      361 ttgccaatgg attcgcattt ttgtccattt tacttttaga aattttatca tgaatttgcg
      421 aataagctgc gatataaatc gcactctcaa atgctggatt ttcaccaaaa gctagtgaat
      481 aaaatttcat catatcatta taattttgct catgagttgc attttcaaag tacatacaaa
      541 cacccctatt ttcttatttt tgtttgctcg attgttcgat ttgctttttt aactcatcat
      601 tttctttttg taattcctca atggcattga acattacctt caacgctgga atctcagttg
      661 taattgtttt gaatatctca ctctctttaa caattttccc tgctcggcga tcatcctcgc
      721 cacttagcca ttcggcttta atacgatttt ctcgcaccca acgttgtgca atcctacgat
      781 ttttagctag tccaaaattc actaaaatat ttgaaactga ttctaaatca tgtgccatgc
      841 cacttgttct ttttaagaaa atgtcacgat tctcttcaat gattttgtgc ttattttttg
      901 atgccataat aacaacctcc ctatgatttt aaacgcattt ctatataaca tcgtatgtat
      961 tggctattgc ggttttggta gaaatcagaa atggataata tgtcataatg cttcttcaag
     1021 tcctcaatca tttcctgcat ttcttgagat aaaccttgca aacgaatttt gatcatattc
     1081 aattcctccc ataattccat tttacaacaa agtcatacaa agttcaacct taaactttat
     1141 attttattta tatatgtatc atgcaaggca tgatggttgt gagtggtacg gtgtaggtgt
     1201 gtctttttag ctgtatagct gtgcttccca agcacggcac agtaatccag tgacacccca
     1261 gcaccccacc tctagcaacg ctacgcaaat ttaatggtaa gcttacatgt gttttataat
     1321 tgatttctat cattcttcaa cgctatatct agaatgtcaa agacaggcac acagccacgc
     1381 catgatttct cacaacggaa tcctatacaa tgtttcaatc gttgcttcat ggtcaatttt
     1441 acgcctaatt gcttctcaat ttccgttgct gttatgccat attccacgat ttcataacca
     1501 ttcttcaaaa catcttcctt gcggaatcgc ccataacgat taggtttcaa ttgaaccacc
     1561 tccaccatga aaattcataa attagcgaat atcacgaaca aacttactag caacatctac
     1621 atattttact ccatcttttg tatcaaactt ctttgtttca attgaaatat ctaactcaac
     1681 ctcatatgta ttccctacta ctaatccatc aactttatta ccaatacaaa ctaccatacg
     1741 tttcttaatt gctggtactt caatcaatgt ataaagtgaa actttctctt tctcatcaac
     1801 acctgcaacc atatactttt gtgtaactga ataacgttct ttcatgtttt aagcacccct
     1861 tttttaatat aaatttgtta aattataaat acatttttct tcttgtcaag tttttctttc
     1921 tttttccttt atataacata caaagttgaa ctttatacgt tttacattta tttattttaa
     1981 ttttatttta ttttatcctt tttttatttt atattttttt actcagaaaa agacaaggtt
     2041 tcccttgcct tttatagaaa ttaatataag ataaaataaa tttactcttg ttcttgtttt
     2101 aacaatgttt ttaccttgtc atccactttg tttatcatta caacaacatc agtttttgcc
     2161 ctggtgatcg ctgtatacaa taggtttcta ttgagtaata atgttttggt tgcatccagt
     2221 aataccaata ctttgtcata ctctgaacct tgtgatttat gaacagttaa gcaatgattt
     2281 ggctctactc tattaaagaa ttcataatct tttagaacaa cctcattatt gttttccgtt
     2341 ctaattgtta cgccaaattc attgtaatca ataatttgcc ctttttcacc attcacataa
     2401 cgtttatatt ggtcattttt gataaatatg atattatcaa attgagcata attaaatttt
     2461 gattgaattt gttttttatc tttttgaaat aaattgttta tccagtcttt taacgctacg
     2521 gagttcgata gaacttggta gcctcttttt ttatattcag caattttttc ttcgctgttt
     2581 ttaaaatcca tcacttcaaa tggaactttt tctatatctc cattcctcac agaagttgct
     2641 acatcaatga ttctgtcacc gcttcgcaat acttttgtta attcataaac accagcaaaa
     2701 tcctttaaat aaaagtatgg gtgttttgaa tgtattggat tgagttgctt tggatcacct
     2761 acaaacaata cttttgttgt tggtttcaat gaagccaaaa atgaagttaa cactcttata
     2821 tcaatcaatg agaactcatc aatgattaca aatgaatatg gcaatggttt gctccagtca
     2881 ttataaaata ttggataatc aatcattgaa tgttttgccc cacagagttt gtgtattgtg
     2941 tttatttcca aattatcaac attaatattt tgttctttca acatgcttct aactctttgt
     3001 gttgcttgtg ctgtatagct tacaacagct acatcatgtt tttcaaagtc tatataatcc
     3061 aacaatgttg atagtgtata agtttttcct gttcctgcat atccaatcaa aaatgaaaat
     3121 aaatcacttg aagtaatccc ttgtaaggct tcaaattgtt ctgttgtcaa tgaaccctgt
     3181 ttcaatgttt ttcgcaactt caattgtttt ggtacaaaaa tttctttact tcttctttca
     3241 agttctaact tcaaagcatt ttgtaacaat tcattttcat ttgccttcaa ttcatctttt
     3301 aatatttctc tatctctatt tgataatccc tttttccttg cttctttgaa gtaatcttta
     3361 aaacaagaaa ttgttttatc aatatattga tttgggtgtt catctgctat gctatcaaga
     3421 ataaaatatt gaaattcttg aatcgttgaa aacttcatat aatgttgtgg caacacacct
     3481 tttacaacac tcaacaattg aaatttccct ttatatggag tttcgaaagc ttcttccaac
     3541 acaattgaaa tattatcact ggaagatata atgttcacac ggtcttcgcc ttcttctttc
     3601 aatttctcat aaacttctaa actagatttt gtaatgtact ttgatacata tctagcaaca
     3661 tattgatgaa aatcatgttc ttcttcacat ttctcttcca aattttcact tgctaaatta
     3721 ctaatttcag ccatgtcata cacatgattt cccatgacac ttttcaataa tttaattggg
     3781 atatagaaat taaaaagaga atgaatgtgt gctgcgtcaa cagtattgtg aaattcaatc
     3841 gttcgaatgt acgccatttc tgtattttcc tttggaattt tcgaacgata aatcgttctt
     3901 tcttcttcat ttggatacaa ttgtgtgtac atattcattg caatatcagt aagcaaaaca
     3961 tgaaaatcat tcaaaaattc ttcacgtctt ccttcttttt tagtatctgc ataactccat
     4021 ccaacccact tttttctttg gctttcaaca ttgtctttat aatatttttc taattggttt
     4081 tttcggagtt ttttgccctg cttcatttcc aaaattattt taatttggtc ttgaagtctt
     4141 gctggacgtt gcgttatcat ttttgtgatt tgataaccaa tgttactgat tggaacataa
     4201 tttatatttt tctctttttt actcaaatct tttccatata accatcgata ataatgttct
     4261 tgatcataaa gagaatggac atattcataa tactttttct ctgcaatttc tcttgttgag
     4321 cgttttcgac ttatataacg tttgataaaa tattcttttt ctaattctct tgttttatca
     4381 accaaacttc caaatttctt caaaagattt tttatgtctg aatcacccat tgtgattgtt
     4441 gcaaaatgtt tcaaatcaaa ttttttggtg tacattgcaa ttttattttg tacatatgtt
     4501 tgtattcttg ttccacaaga atcacaatcc attctcttgc attgtgcaac aaaaccatac
     4561 atctcctttg ttgctttatc ctgtaccaaa acactctgtt tcccacattc atgcgtttcc
     4621 atcgactctc cctcccttct tgttgtctaa ttgagaatga aaaaaaacgt acatggcttt
     4681 tttgccccga aacccaacgg gctgtaaggc tcatagctgt tttctaattg agaaagtgac
     4741 ttttgtgcgg gtattttcgc cattactgta atcaagttaa gggaaaagtc gctcacctca
     4801 acttttcatt gcaacttttt gtttctcatg gtctactaaa ttacttaata aagcattgat
     4861 cttatcacgt tcttctgaat ctactaaatc cataatgttt tttattgtat cttccatgta
     4921 cgagaaacga actacgtttt gacgatatgt tttattcatt cttggtcttc ctcttttggc
     4981 tttctgcatc tatcacacct cctttctatt tttttgttat acaataaata gtttagcgca
     5041 aaattgcaaa attcctagtt ttttttgcaa aaatttttac tttcataggg aatccgcttg
     5101 ttcctcgtca aatgataggt agtatttttg cgaagaccat gcaaaaatct ccacctaaac
     5161 atcatttgac aagtaccatt ttggaacgct ttttgtcatg caccaagccg acataccaaa
     5221 aagctcacta atttgttttc gtcttgtttg cctattgtag cgtcccaaaa acgctcggca
     5281 aaagcaaaa
//
