LOCUS       NZ_AP022876             8319 bp    DNA     circular CON 13-MAR-2024
DEFINITION  Bacillus cereus strain MRY14-0057 plasmid pMRY14-0057_2, complete
            sequence.
ACCESSION   NZ_AP022876
VERSION     NZ_AP022876.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00203323
            Sequence Read Archive: DRR206363, DRR206387
            Assembly: GCF_021654895.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8319)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 316x
            Sequencing Technology             :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021654895.1-RS_2024_03_13
            Annotation Date                   :: 03/13/2024 06:47:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,880
            CDSs (total)                      :: 5,728
            Genes (coding)                    :: 5,499
            CDSs (with protein)               :: 5,499
            Genes (RNA)                       :: 152
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 106 of 229
            Pseudo Genes (incomplete)         :: 133 of 229
            Pseudo Genes (internal stop)      :: 47 of 229
            Pseudo Genes (multiple problems)  :: 51 of 229
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022876.1.
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8319
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0057"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0057_2"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            664..861
                     /locus_tag="L6489_RS29200"
                     /old_locus_tag="BCM0057_p201"
     CDS             664..861
                     /locus_tag="L6489_RS29200"
                     /old_locus_tag="BCM0057_p201"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788725.1"
                     /translation="MIGKDMITLDNPKKEYFQQYAQNNKEQINANARRRRQQQKEKVEK
                     YDRLVAALRSRGISVEELLK"
                     /name="hypothetical protein"
     gene            1284..2384
                     /locus_tag="L6489_RS29205"
                     /old_locus_tag="BCM0057_p202"
     CDS             1284..2384
                     /locus_tag="L6489_RS29205"
                     /old_locus_tag="BCM0057_p202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205124.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_098492605.1"
                     /translation="MNHEEFINSTEIDDKFLKQIIDNREFNDVIVSYYERLHKESRDDG
                     MKKKQENVKGCNDFWIIDKYHEHRLKEFKRTNLCKDKFCNNCKKVKQASRMGKFIPLIQ
                     PYAENMYQLTLTIPNVKGDELKDTLSKMQKAFARLIEYFKGKKKINGIDFEVLQYEGAI
                     RSLEITFNGNEYHPHYHALIVLKVNKFHDCMIRKHKNVYSIDFTGKREARLFSDIEVLI
                     QKVWYLIINGKRITETAIQSLKKGYSCQLDKFSENDFVEIFKYMTKATTEDGKTLSYKQ
                     FKTLYYALFKVRQIQGYGIFYNLKDDEEINFDEVNEAYAEFIEELQTKEKPVTVSEAPE
                     QVRKESDYTVFSRKTFQKYWKQVMSE"
                     /name="protein rep"
     gene            2852..3001
                     /locus_tag="L6489_RS29210"
                     /old_locus_tag="BCM0057_p203"
     CDS             2852..3001
                     /locus_tag="L6489_RS29210"
                     /old_locus_tag="BCM0057_p203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003205126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788727.1"
                     /translation="MEREKCSLKEAKNAYNRKWRAANRDKVKLYNDRYWEKKAKEMEEQ
                     ACQK"
                     /name="hypothetical protein"
     gene            2989..3261
                     /locus_tag="L6489_RS29215"
                     /old_locus_tag="BCM0057_p204"
     CDS             2989..3261
                     /locus_tag="L6489_RS29215"
                     /old_locus_tag="BCM0057_p204"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788729.1"
                     /translation="MSEVSMETVIKGKQQSELLKHLEKVGIELLGRRDELLEQWEKEGL
                     KEDSIFEDDLKFVEELMNRNDELMFDVKVELITTMDEIHHQKMGY"
                     /name="hypothetical protein"
     gene            3266..3517
                     /locus_tag="L6489_RS29220"
                     /old_locus_tag="BCM0057_p205"
     CDS             3266..3517
                     /locus_tag="L6489_RS29220"
                     /old_locus_tag="BCM0057_p205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788731.1"
                     /translation="MLGKYDGLLEKERLLKRIDELSFALEASGDPMLEAMVKNSGNTDI
                     AVAKELITKGSELGMNEHVFKKALLAKVNELREEVTKL"
                     /name="hypothetical protein"
     gene            3514..3927
                     /locus_tag="L6489_RS29225"
                     /old_locus_tag="BCM0057_p206"
     CDS             3514..3927
                     /locus_tag="L6489_RS29225"
                     /old_locus_tag="BCM0057_p206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788733.1"
                     /translation="MKKDKQKDLPFNHKVVLSVLPLGRSKAITTKEIMARSGIKDKRAV
                     SAILSELVTVYKQPIATTSKKEFKGVFIVQDVIDYQIGKEGLVSRKRHIDKRDSAYTEA
                     CLEIFKGTVTLDESKNKVEGSVRACKQSASSSM"
                     /name="hypothetical protein"
     gene            3897..4604
                     /locus_tag="L6489_RS29230"
                     /old_locus_tag="BCM0057_p207"
     CDS             3897..4604
                     /locus_tag="L6489_RS29230"
                     /old_locus_tag="BCM0057_p207"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788735.1"
                     /translation="MQTISKFFNVDNESKLNELTIAKGELNSKESELQSKLSRVERALE
                     LAQTDLMIDDSTANKKVVAKYEEAQEKFQSELIATQEELNKVSDEIAAIVKEELHAELV
                     DAAKQDVMLIELPEKCRKAKKVINRDLYRATSFEDEGRFSRKYLHVSATVIDDARTFAD
                     IHRFADKPNLTRIGYQRGELIRWGGDNNLFKLNDEYETIYEEEAQKIKDKTDKEYEQFM
                     KAFNKYFAVSDSH"
                     /name="hypothetical protein"
     gene            4658..5083
                     /locus_tag="L6489_RS29235"
                     /old_locus_tag="BCM0057_p208"
     CDS             4658..5083
                     /locus_tag="L6489_RS29235"
                     /old_locus_tag="BCM0057_p208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788712.1"
                     /translation="MSKDTLKNIFHVYCFYTVRLQGDTEPRMRRNKLNYDNPIQNYYES
                     FIDCLRGFCEVRSDVSLYSFYQFVVDAVNSLNKQERKLIYERYLYKDHYKSDRQHYLAM
                     DITPQNYKKQMDPVRRKLIKNLGIEDLQLNIPDWMKR"
                     /name="hypothetical protein"
     gene            5214..5462
                     /locus_tag="L6489_RS29240"
                     /old_locus_tag="BCM0057_p209"
     CDS             5214..5462
                     /locus_tag="L6489_RS29240"
                     /old_locus_tag="BCM0057_p209"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_236788716.1"
                     /translation="MKSLYRILELDKDEVSSSEKLILLTIAIYSDNKFIPFSITELENY
                     TNLSRATVTRLVKQLEEKGFVEVQRNGTATNNYKVTL"
                     /name="helix-turn-helix domain-containing protein"
     gene            5539..5715
                     /locus_tag="L6489_RS29245"
                     /old_locus_tag="BCM0057_p210"
     CDS             5539..5715
                     /locus_tag="L6489_RS29245"
                     /old_locus_tag="BCM0057_p210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176543923.1"
                     /translation="MLPQKEQLGHLAEKTELVKDLAWIAHDLLTEEDYTKENAAEALIK
                     VINRELSYVSKVR"
                     /name="hypothetical protein"
     gene            5757..5963
                     /locus_tag="L6489_RS29250"
                     /old_locus_tag="BCM0057_p211"
     CDS             5757..5963
                     /locus_tag="L6489_RS29250"
                     /old_locus_tag="BCM0057_p211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788718.1"
                     /translation="MANTVAKDKFEIVEKFVNACMLKGVSEDKSFKLVEELCTLIQQKR
                     HDEWFGLVDEALKEAGHEEGVTL"
                     /name="hypothetical protein"
     gene            5960..6091
                     /locus_tag="L6489_RS29415"
                     /old_locus_tag="BCM0057_p212"
     CDS             5960..6091
                     /locus_tag="L6489_RS29415"
                     /old_locus_tag="BCM0057_p212"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272880692.1"
                     /translation="MSEVEMVLHEFEELLHKIISAAQHGDEQAMNHLDIVKAWLKDN"
                     /name="hypothetical protein"
     gene            6168..6407
                     /locus_tag="L6489_RS29255"
                     /old_locus_tag="BCM0057_p213"
     CDS             6168..6407
                     /locus_tag="L6489_RS29255"
                     /old_locus_tag="BCM0057_p213"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236788720.1"
                     /translation="MAEEHRYHVTFYFSNGKEFDGKVTSKYNKEDYLEGLEDLFLKEKT
                     LLIDHLGMLIQTKYITHVKVIEVGSKIGADKKDT"
                     /name="hypothetical protein"
     gene            6468..7004
                     /locus_tag="L6489_RS29260"
                     /old_locus_tag="BCM0057_p214"
     CDS             6468..7004
                     /locus_tag="L6489_RS29260"
                     /old_locus_tag="BCM0057_p214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_236788722.1"
                     /translation="MTDKTDSSQSIYISKDVAMMLKIQESTLRKYCIMLEEHGYKFHKN
                     EHGHRGFLDNDVITLRKLIEIKSHPDMTLKQACNAIMTWVKEKDMSEVDTNVITENEQH
                     DERYSELKEMIQQQNELLKKMAKKLDDQQRYIDEKLEKRDQQLMGAIREMQEEKRVLLE
                     TASASQEKKWYKFWK"
                     /name="DUF3967 domain-containing protein"
     gene            7628..8227
                     /locus_tag="L6489_RS29265"
                     /old_locus_tag="BCM0057_p215"
     CDS             7628..8227
                     /locus_tag="L6489_RS29265"
                     /old_locus_tag="BCM0057_p215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562260.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_236788723.1"
                     /translation="MNKRFAYIRVSDKTQNENRQLDEMLQQEIEERDIFIDKASGKDFN
                     RPKYQALKQMLRAGDILYIKSIDRFGRNKDDILKEWNEITKEIGADIVVLDMPLLDTRQ
                     YKDVLGSFVSDLVLQILSFFAEEERRKIKERQREGIDAAQRRGAKFGRPAATFPPGFEE
                     VYKRWKDGEIKAIEAMEELELKRTTFYKLVNSFENK"
                     /name="recombinase family protein"
ORIGIN
        1 tacacataat tttatgcctt tcatgtcgaa aagtaaaagt taaaaaagta ataaaaaagc
       61 aaagtataaa aagtatactt ttaagtttgc ttgtttctat gaaaatccgt tggtataata
      121 gttttaacgt gtttgagtgt ttcaaacgaa aagaaaaaag ccgagttggg gaactcgact
      181 taagtctttc taaaagtatg gggatacttt tagattagat tttgtgtctc gacaacactt
      241 tcgactaatt agcactacca atgcttatta gtcattgatg attttacgaa tcatcttaac
      301 gattattttt ttatggattg acacctattt tatcgacctt tgacaatcaa ataattaggt
      361 accatttata tctaagattg aattaacaac cttatttaac gtatgaaaaa gtactgtagg
      421 tcaagttgta aactataaaa agtttcttct taggaaacct taagagaaaa aaatatagat
      481 acaatttgta tcgctttaat acgaaaaaaa gttctcgttc agcgaacttc ctatgtgaca
      541 agtttacaat gtatttttcg ttttgacaat actttaagta aactcatagt ttttacatac
      601 attgaaaggt acctctacta tttgaggtgc cttttttatt tatttttgaa aggatgatag
      661 aaaatgatag ggaaagatat gatcacatta gacaacccaa agaaagagta ttttcagcaa
      721 tatgcacaaa ataataaaga acaaattaac gccaatgcaa gaagacgaag acaacaacaa
      781 aaagaaaagg tagagaagta tgatagattg gttgcggcgt tacgatctag aggaattagt
      841 gtagaagaat tattgaaata agatggacaa gctgtattaa ggccggacta agacaccagg
      901 agaagatttg tatacggtct aggttattcg tggggcttat gctacagctg tcattgtcta
      961 gcggtatcgc agtgcgtaag cacgagaata aggagcagac atacaccaca agccccgaac
     1021 actttcatgc tctccaaaac aacatgtttt ggggatacct agtgtaacgt ggccgagtgc
     1081 aacggaaggt tttaatgttt cgtttaaaaa atgagtttct tctattactt gatatattag
     1141 aaataagtcg gctatttctc tagttttcta gtaatgacaa attgtcgata accatagtca
     1201 tatcaagggt ttaagctatt cgacttattg ctaataaggc ttaaaaagcc ttccaaaaat
     1261 tccactagaa atgaggtgag aaattgaatc atgaggaatt tataaattca actgaaatag
     1321 atgataaatt tttaaagcaa atcattgata atagggaatt taatgacgtt atagtctcgt
     1381 attatgaacg actacataaa gaatcacgcg atgatggaat gaagaagaag caggaaaacg
     1441 ttaaaggttg caatgatttt tggattatag acaaatatca tgaacataga ttaaaagagt
     1501 ttaaacgaac taatctttgc aaggacaagt tttgtaacaa ctgtaaaaag gttaaacaag
     1561 cttcacgcat gggtaaattc ataccactta ttcagcctta cgcggaaaat atgtatcaat
     1621 taacgctaac tattcctaat gttaaaggcg atgaattgaa agatacacta agcaagatgc
     1681 aaaaagcttt tgctcgttta attgagtatt tcaaaggcaa aaagaaaata aacggaattg
     1741 attttgaagt tttacaatac gaaggcgcta tacgatcttt agaaataaca tttaacggta
     1801 atgagtatca tccacattat catgctctta ttgttttaaa ggttaataaa ttccatgatt
     1861 gcatgataag aaaacataaa aatgtttatt caatagactt cactggaaaa cgtgaggcgc
     1921 gtttatttag tgatatagag gttttgatac agaaggtttg gtacttgatt ataaacggta
     1981 aacggataac agaaacagcg atacagtcac ttaaaaaagg ttattcatgt cagttggaca
     2041 agtttagcga aaacgatttt gtagagatat ttaagtacat gacaaaagca acaacggaag
     2101 acggtaaaac attgagttat aagcaattca aaacgttgta ttatgcgtta tttaaagtta
     2161 gacaaataca agggtatggc atcttttata atttgaagga tgatgaagaa attaattttg
     2221 atgaagttaa tgaagcgtat gcggaattca tagaggaatt acagacaaaa gaaaaacctg
     2281 ttacggtatc agaagcacca gaacaggtca gaaaagaaag tgactataca gtgtttagtc
     2341 gaaaaacgtt ccaaaaatat tggaagcaag tcatgagcga atagccttac tcattccggc
     2401 aagtttgata aaaggctatc tttaccacca ttaatcaaat aacggagggt tttctaatat
     2461 cattatacat aattttaatt cgtatgatat taggaaattc cttctatatc gttaattagt
     2521 agagactatg aattatttcg tagtcttttt ttattggaaa ggaatgattg ttgttatatt
     2581 tagaggttta taacaggaaa aaggcgcctg atctcgtttg aatcagaacg aaatcaagcg
     2641 cctttgggcg ataattaacc aagaacaagt taattaaata aatctaaaag tttttggaag
     2701 tacttatatt atatcgtata tggagttccc ggacagtaat tttagcctaa atttgcgtga
     2761 agcaaatcat taatcatacc acgtaaacta tatattcgta aataggtaaa tgttaaaata
     2821 agagtaagaa gaatgaaaag aaggtggaaa agtggaaaga gaaaagtgtt ctttgaaaga
     2881 agctaaaaac gcttataacc gaaaatggag agcagcgaat cgcgataaag tgaagctgta
     2941 taacgataga tactgggaaa agaaagctaa agaaatggag gaacaagcat gtcagaagta
     3001 agtatggaaa ctgttattaa aggaaagcag cagagtgaac tgttgaagca tttagaaaaa
     3061 gtaggtattg aattactggg gcgtagagat gaattgttag agcagtggga aaaagaagga
     3121 cttaaagaag acagcatttt tgaagatgat cttaaattcg ttgaagaatt aatgaaccga
     3181 aatgacgaat taatgtttga tgtaaaagtg gaattaatta caactatgga tgagattcat
     3241 catcagaaaa tggggtattg ataggatgtt aggaaagtat gatggattat tagaaaaaga
     3301 acggttactt aaacgaattg atgaactgtc attcgcgtta gaagcttcag gtgaccctat
     3361 gttggaagcg atggttaaaa atagtggcaa tacagacatc gcggttgcaa aagagttgat
     3421 tacaaaaggg agtgaattag gtatgaatga gcacgtattt aaaaaagcgt tgctagcaaa
     3481 agtaaatgaa cttcgtgaag aggtaacgaa actatgaaaa aagacaaaca aaaagacctc
     3541 ccttttaatc acaaagtagt attgagcgta ctacctttag gaagatctaa agcaataaca
     3601 actaaagaaa ttatggcacg tagtggaata aaagacaagc gtgcagtatc tgctatttta
     3661 agtgaattag tgactgtata taaacaacct attgctacaa cgtctaaaaa agaatttaag
     3721 ggcgttttta ttgtacagga tgtaatagat tatcagattg gtaaagaagg ccttgtgagc
     3781 agaaaaaggc atatagataa gcgtgattca gcttatacag aagcgtgctt ggaaatattc
     3841 aaaggtacag tgacattaga tgagtcaaaa aacaaagtgg aaggaagtgt tcgtgcatgc
     3901 aaacaatcag caagttcttc aatgtagata acgagagtaa gttaaacgaa ttaacaatag
     3961 caaaagggga attgaattcc aaagaatcag aattacaaag taagctatct cgagtagaaa
     4021 gagctctgga actggcacaa actgacttaa tgattgatga tagtacagct aataaaaagg
     4081 ttgttgcaaa atatgaagaa gctcaagaga agttccaatc agagttaata gctactcaag
     4141 aagaacttaa caaggtttct gatgagattg cagctatcgt taaggaagaa ttgcatgctg
     4201 aattagtaga tgccgctaaa caggacgtta tgctaatcga actacctgaa aagtgcagaa
     4261 aagcgaaaaa agtaattaac agggatcttt atagggcgac gtcctttgaa gatgaaggtc
     4321 gatttagtag aaagtatttg catgttagtg ctacagtgat cgacgacgct cgtactttcg
     4381 ccgatataca tcgttttgct gacaaaccaa atctaacacg aatagggtat caacgcggtg
     4441 aattgatacg ttggggtggc gataataacc tgttcaaatt gaatgatgaa tatgaaacga
     4501 tatatgaaga agaagcacag aaaatcaaag acaaaacgga taaagagtat gagcagttca
     4561 tgaaggcttt caataagtat ttcgctgtgt cggattctca ttaatcaaaa tacataagtc
     4621 gcctaagggc ggcttttttt tattaggagg gattttcatg agtaaagata cattaaaaaa
     4681 catctttcac gtgtattgtt tttatacagt acgtttgcaa ggcgatacag aaccacgtat
     4741 gagacgaaac aagctaaatt atgataatcc tatacaaaac tattatgaaa gttttataga
     4801 ctgtctgaga ggattctgtg aggttaggag cgacgtttca ttgtattcgt tctatcaatt
     4861 tgttgtagat gctgttaatt cgttaaataa gcaggaaaga aagctgatct atgagcggta
     4921 tttatataaa gatcactaca aatcagatag gcaacattac cttgcgatgg atataacacc
     4981 acagaattat aaaaaacaga tggatccggt aagaaggaag ttaattaaga acctcggaat
     5041 agaagattta cagttgaata ttccagattg gatgaaacga taacgggtta aattacatgt
     5101 atggttatat tatttttaaa ttttattttg attctattgc attttactat actcattgtt
     5161 atactagata atgtaaacaa catgttgatt tataggaggt tactagttta attatgaaat
     5221 cactatatcg tattcttgaa ttggacaaag atgaagtttc ttcttctgaa aaactaatct
     5281 tgctaacgat agcgatctat tcagacaaca agtttatccc attctctatt acagaattgg
     5341 agaattacac gaatttgagt agagctacgg ttacaaggct cgtaaaacag ttagaagaaa
     5401 agggattcgt cgaggttcaa aggaatggaa cagcaacaaa caattacaaa gtaacactgt
     5461 aaaaacaaag aaagagcagg agattgcggc tcctactctt tcaaaacaca ttatccctta
     5521 tggaaaggat gtattagtat gttaccacaa aaagaacagt taggacatct tgctgagaaa
     5581 actgaattag ttaaggattt agcttggatt gcacatgatt tacttaccga ggaagattac
     5641 accaaagaga atgcagcgga agcgttaatt aaggtcatta atagggaatt atcatacgtt
     5701 tccaaagtgc gttaaacggg attatatgta aataaaacaa ttcggaggta atattcatgg
     5761 caaatacagt tgctaaagat aaatttgaga tcgtagaaaa gtttgtgaat gcttgtatgt
     5821 taaaaggtgt ttcggaagat aaatcattca aattagtaga ggaattatgt acacttattc
     5881 aacaaaaacg tcatgatgaa tggtttggat tagtagatga agcacttaaa gaagcgggac
     5941 atgaagaagg tgtaacgtta tgagtgaagt agaaatggtt ttacacgaat tcgaagaact
     6001 attacataaa atcatatcag cagcgcaaca tggcgatgaa caagcgatga atcatttaga
     6061 tatagtaaaa gcatggttaa aggacaatta aggcgtggtg atccacgtct tttttttata
     6121 ctgtcttggg gaatcctact gatatatttt gaaagtaggt ggactaaatg gctgaagaac
     6181 atagatacca tgtaactttt tatttttcta acggaaaaga atttgatggt aaggtaacaa
     6241 gcaaatacaa caaagaagat tatttagaag ggttagaaga tctattcctt aaagaaaaga
     6301 cattactgat tgatcatcta ggaatgttaa tacaaaccaa atatattaca catgtcaaag
     6361 ttatcgaagt tggatctaaa ataggagcag acaaaaaaga tacgtgaatt attaaggaaa
     6421 aggatatatc attgttataa cgaatgtttt ggtagaggtg aagcgttatg acagataaaa
     6481 cagacagctc tcaatccata tatataagca aagacgtagc gatgatgctt aaaattcaag
     6541 aatcgacttt acgtaaatat tgtatcatgc ttgaggaaca cggctacaaa tttcataaga
     6601 atgagcacgg ccatagagga ttccttgata acgatgttat aacgttaaga aaattaatcg
     6661 agattaaatc acatcctgat atgacattaa aacaggcctg taacgcaatt atgacgtggg
     6721 ttaaagagaa agatatgtca gaggttgata cgaatgttat aacggaaaat gagcaacatg
     6781 acgagcgata tagtgagctt aaagaaatga ttcagcagca gaatgaatta ttaaagaaaa
     6841 tggcgaagaa attagatgat cagcagaggt atattgatga aaagttagaa aaacgagatc
     6901 agcagttaat gggagctatc cgagaaatgc aagaagaaaa acgagttttg ttagaaacgg
     6961 cttccgctag tcaagaaaag aaatggtaca agttttggaa gtaggattcc ctaactatgg
     7021 ggagtccttt tttattttaa agtgagtttg gatcacttag aggtagatta aacagaaagc
     7081 cctgcaggta acatcctcag aaacgcacaa gaaaagtggt gcgtataact gggcactcgc
     7141 ttttttctcg tgaataagat ctcattgaat ggagtaattt tgtatactac tccgttggaa
     7201 tatagaacgt tttacggagc gttccaatat cgttaccttt gcaaaacaaa cacgaatacc
     7261 acgttacttt aatccgcaca caaggcaccc tttttatgca gtactcattg tagtattgca
     7321 ccccttttaa tgttgtactg caaaacaaac acaactcaac taataaagaa caaagcacat
     7381 ataccggtaa ttgaatggta acgggtgtta aggaatgata aggagtatta gtgcaaaatg
     7441 aatgtagtag tattttagta atttgcaaaa caaacgaact caattaatgt attcgtaaaa
     7501 gtacacattt acgaataaga aaaagaataa gaaaaagata ttgttttgtg ttcgtataag
     7561 ttgtatgatt agtttacaaa cattcgtaaa ttgaaaaaca cttttacgaa caaaggggaa
     7621 gaggaaaatg aacaaacgtt tcgcttatat ccgtgtatca gataaaactc aaaacgaaaa
     7681 tagacagttg gacgagatgt tgcagcagga gattgaagaa agagatatct tcattgacaa
     7741 ggcaagcggt aaagatttta atcgtcctaa ataccaggct ttaaaacaga tgctacgtgc
     7801 cggagatata ctgtatatca aatctattga tcgttttggt agaaataaag atgatatttt
     7861 aaaagagtgg aatgaaataa cgaaggagat cggtgcggat atcgttgtat tggacatgcc
     7921 tttattagat acaagacaat ataaagatgt attaggctcg tttgtaagtg atttggtact
     7981 acagatacta tcattctttg cagaagaaga aagacggaaa atcaaagaga gacaacgtga
     8041 gggcattgat gccgcacaaa gacgaggtgc gaagttcgga agaccggctg ctacattccc
     8101 gccaggattc gaagaagtgt acaagcgttg gaaagatgga gaaattaaag caatagaagc
     8161 tatggaagaa ttagagttaa aacggactac tttctataaa ctagtaaatt cattcgaaaa
     8221 taagtaaaaa aagtaataat aatattaaac tttttacaaa attaagtcga aaaagcaaag
     8281 ttaaaaaaag acaaacatac cgataacaag cattgatat
//
