LOCUS       NZ_AP022819             7939 bp    DNA     circular CON 03-MAR-2024
DEFINITION  Escherichia coli strain JE86-ST05 plasmid JE86-ST05_p4, complete
            sequence.
ACCESSION   NZ_AP022819
VERSION     NZ_AP022819.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00197820
            Assembly: GCF_015509005.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Kimata,K., Lee,K., Watahiki,M., Isobe,J., Ohnishi,M. and Iyoda,S.
  TITLE     Global distribution of epidemic-related Shiga toxin 2 encoding
            phages among enteroaggregative Escherichia coli
  JOURNAL   Sci Rep 10 (1), 11738 (2020)
   PUBMED   32678145
  REMARK    DOI:10.1038/s41598-020-68462-9 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Kimata,K., Lee,K., Watahiki,M., Isobe,J., Ohnishi,M. and Iyoda,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2020) Contact:Ken-ichi Lee National Institute of
            Infectious Diseases, Deaprtment of Bacteriology I; 1-23-1,Toyama,
            Shinjuku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Coverage                   :: 135.0X
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/03/2024 12:11:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,632
            CDSs (total)                      :: 5,499
            Genes (coding)                    :: 5,174
            CDSs (with protein)               :: 5,174
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 166 of 325
            Pseudo Genes (incomplete)         :: 170 of 325
            Pseudo Genes (internal stop)      :: 58 of 325
            Pseudo Genes (multiple problems)  :: 63 of 325
            Pseudo Genes (short protein)      :: 5 of 325
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022819.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JE86-ST05"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="JE86-ST05_p4"
                     /geo_loc_name="Japan"
                     /collection_date="2014"
     gene            complement(join(7651..7939,1..410))
                     /locus_tag="JL003_RS27745"
     CDS             complement(join(7651..7939,1..410))
                     /locus_tag="JL003_RS27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000939065.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     gene            complement(958..1116)
                     /locus_tag="JL003_RS28505"
     CDS             complement(958..1116)
                     /locus_tag="JL003_RS28505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            1446..1633
                     /locus_tag="JL003_RS28510"
                     /pseudo=""
     CDS             1446..1633
                     /locus_tag="JL003_RS28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1776..1967)
                     /locus_tag="JL003_RS27750"
                     /old_locus_tag="JE86ST05C_10"
     CDS             complement(1776..1967)
                     /locus_tag="JL003_RS27750"
                     /old_locus_tag="JE86ST05C_10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2032..2355
                     /locus_tag="JL003_RS27755"
                     /old_locus_tag="JE86ST05C_20"
     CDS             2032..2355
                     /locus_tag="JL003_RS27755"
                     /old_locus_tag="JE86ST05C_20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955993.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2564..>2995
                     /locus_tag="JL003_RS28295"
                     /pseudo=""
     CDS             2564..>2995
                     /locus_tag="JL003_RS28295"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3033..3551
                     /locus_tag="JL003_RS27765"
                     /old_locus_tag="JE86ST05C_30"
     CDS             3033..3551
                     /locus_tag="JL003_RS27765"
                     /old_locus_tag="JE86ST05C_30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            3558..3791
                     /locus_tag="JL003_RS27770"
                     /old_locus_tag="JE86ST05C_40"
     CDS             3558..3791
                     /locus_tag="JL003_RS27770"
                     /old_locus_tag="JE86ST05C_40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4501..4740
                     /locus_tag="JL003_RS27775"
                     /old_locus_tag="JE86ST05C_50"
     CDS             4501..4740
                     /locus_tag="JL003_RS27775"
                     /old_locus_tag="JE86ST05C_50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            4737..5099
                     /locus_tag="JL003_RS27780"
                     /old_locus_tag="JE86ST05C_60"
     CDS             4737..5099
                     /locus_tag="JL003_RS27780"
                     /old_locus_tag="JE86ST05C_60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(5124..6017)
                     /locus_tag="JL003_RS27785"
                     /old_locus_tag="JE86ST05C_70"
     CDS             complement(5124..6017)
                     /locus_tag="JL003_RS27785"
                     /old_locus_tag="JE86ST05C_70"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(6020..7612)
                     /locus_tag="JL003_RS27790"
                     /old_locus_tag="JE86ST05C_80"
     CDS             complement(6020..7612)
                     /locus_tag="JL003_RS27790"
                     /old_locus_tag="JE86ST05C_80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     Typing          1613..1700
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1045..1593)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.479"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2000..2349
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.42e-67"
     Typing          2346..3895
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.595"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tattcgttaa aacgatattc ttggaatgtt ataccatgct cattaacatg ccttatctca
       61 ttttttagca tgtcatttgt atttggataa tggtggaggt aagcaccaag atatttttca
      121 caaaagctgg tataagcatc tgtaaattga ataaacgtat gccacatttc atctataatt
      181 tgtaattgct cgtgaacttt tatattgtag cattccgtta ggcatgttga agctagccag
      241 atgaatttct ttgtttcatt aaagatatca tcagcttctt ctggtgttac attccattct
      301 ttacgaaatt ttagaagaac atcatcattg tgataactca tgatatcttc taatgagcta
      361 acattatgaa cttttgcata agaactgctg ttcatcttat gttgtagcat aacaccccca
      421 attatatatt tgaatgtaga tgagaatagt tatcatttgt tatagtattg ttaaatttgt
      481 gcttgtgtca atatgcctaa ttctataaat gagatagtaa tcaagttttt taatggttat
      541 aggatccatt agtaatacaa ggtttttgca gccagttttt acgggtaggg gctgcaagga
      601 aaacaaaatt ttcccactct aagagaagag aattgagttg gttgaatgag tggatgcatc
      661 tagggccgct aattcaccga gtgtaaaggc tgtacgaaat gtgttgcagt ccgataatag
      721 ccccctccat tgacgtttta cacatgtgtt gtaggttcca ggagtgggcg gatctcaaaa
      781 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc cagtaaacga
      841 aaaaaccgcc tggggaggcg gtttagtcga aggttaagtc agttggggac tgcttaaccg
      901 ggtaactggc ttggtggagc acagatacca aatactgtcc ttctagtgta gccgcagtta
      961 ggccaccact tcaagaactc ttaatatctc aatccacctt gtccagttac cagtggctgc
     1021 tgccagtggc gctttgtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1081 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1141 ctacaccgag ccgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1201 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1261 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     1321 tgagcgtcga tttttatgat gctcgtcagg ggggcggagc ctatggaaaa acgccaacgg
     1381 cgcggccttt tgttggcctt tgcctttttg ctcacatgtt ctttccggcc ttatcccctg
     1441 attctgtgga taaccgtatt acgcctttga gtgcgctgat accgctcgcc gcagccgaac
     1501 gaccgagcgt agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggcattttct
     1561 ccttacgcat ctgtgcggta tttcacaccg catatggtgc tcttttctta taatctgcac
     1621 tgatgccgca tagttaagcc agtatacact ccgctatccg ctacgtgact ggttcagggc
     1681 ttcgccccga aaccccctga cgcgccctta cgggcttgtc tgctcccggc atccgctcac
     1741 agacaagctg tgaccgtctc cgggagctgt atgtgtcaga ggttttcacc gtcatccccg
     1801 aagcgtgcga ggcagctgcg gtaaagctcg tcggcgtggt cgtgaagtga ttcacaaata
     1861 tcggcctgtt catctgcgtc cagttcgttg agtttctcca gcagcgttaa tgtctggctt
     1921 ctgataaaac gggccatgtt aagggcggtt ttttcctgtt tagtcactga tgcctccgtg
     1981 taagggggat ttctgtttta tggggtaatg ataccgatga aacgcgagag gatgctcacc
     2041 atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg taaacagctg
     2101 gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cacgctccgg gaagctgccg
     2161 acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct gaatcagaca
     2221 gcgaggaagg tgaacagcgg gcagtggtct tccggtgatc gggttcaggt gttggctgca
     2281 ctgatggcca tcggggagga gctgcgtcgg ctgcgtctgg ccgtcaggga acagggcgcg
     2341 cgggatgatt gttaaatttc atgccagggg aaaaggcggt ggcagtggtc cggttgatta
     2401 cctgctggga agggagcgaa accgggaagg ggcaacggtg cttcagggtg atccggaaga
     2461 agttcgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtcag gtgttctgtc
     2521 gttcgcggag aaggagctgc cgccgggagg acgtgaaaag gtgatggcga gctttgagcg
     2581 tgtactgatg cccggtctcg aaaagaatca gtacagcatc ctgtgggtgg agcaccagga
     2641 caagggacgg cttgagctga attttgtcat cccgaacatg gagctacaga ccggaaaacg
     2701 cctccagccg tactacgacc gggcagacag gccgagaatt gatgcctggc agacgctggt
     2761 taatcaccat tacgggctgc atgaccccaa cgccccggag aaccgccgga tactgactct
     2821 ccctgataac ctgccggaaa cgaaacaggc gcttgcagag agcgtcacac gcgggataga
     2881 cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg cgctcacaga
     2941 ggcagggctt gaagtggtca gggtgatgcg aagcagtatc agcattgcag acccgaacgg
     3001 cgggaagaat atcaggctga aaggagcatt ttatgagcaa tcttttacag acgggcgcgg
     3061 agttcgagaa aaagctgaaa gagagagccg aatctacaga gacaatgctg aacgacgagt
     3121 tcagcaagct cgaaaaatct gtaagcaagg ctgtgacatc aaacgagacg aaaatcaaag
     3181 acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacac cgggaagggg
     3241 tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg aatacgggca
     3301 tgatgttact ggggatggtc cttttcctgt ttaccgtgag tggcgggacg ctctggtatc
     3361 ttggcgggat gatacaggcg aacctggaag aaatcaggat acaggaagaa acattgcaga
     3421 aactgaacgc gaagacatgg ggcgtggagt ttgtgcagga cgggagaagg aaatttctgg
     3481 ttatcccgca ggggaaatcg gcaacggtga ttccctatca ggggaaggac tgggtacaac
     3541 tgacggagtg acacagagtg acggagctgg aaagacattt gctgaacgcc ttagagcaac
     3601 tgcaacagga ctatatgcag cggctgaacg aatgggagag cgccttcgtg gaattgcaga
     3661 aaatgttttc gcttacgcaa cgggacaacg cgatgctgaa cgagcgggtc atgcagttga
     3721 gtcagcaggt gcagcacttg agcgagcaga cagaacgctt gagccagtta tacagcgaga
     3781 acaggagata agggatgaac ggctgataca ggaacgcgaa catgagttat ccctggagcg
     3841 tgagcgccag ctggaaatac aggaacggac acaggatggc ccttcgctgg gatggtaatc
     3901 agagacaggc taaaatgttg tcagcgcgtt tccggtgcat tttttgggat aatggttggt
     3961 tcggggcacc gggaacgcgc ttcagtcaga ccttgctgaa aacacaggaa cgcgttaaaa
     4021 cgcatacggg cgcgattctg gtgtgtcttt tgttgctgtg ttttgcctct attgccagat
     4081 cgcgctcatt cccctgaact tctgttttcc cgctccggtc cccgtcttct tcgcttccga
     4141 aagcatgaaa aaaatccgtt tcagctgtct ctggctggca attcctgcgt gattcacatg
     4201 gctgcatagc tatgcatgtg gttaaaattt accgggtgcg atcgcggcag tttttcgggt
     4261 ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ccgaacgcgt ttcagcggtg
     4321 cgtacaataa gggattatgg taaattatct tgttccttag agtatgattc gaggtggtat
     4381 cctgcactta tttaaatatg cttgcttggg aaattccagg ttgcagtatg gttgagttgt
     4441 aattttccta tgataggaaa aatgttatat tattaattta tttgtgaggc ttcataaaga
     4501 atgaaaattt ctgatattga aaaaaacata tataaaggac tagctgaatt tatttcacaa
     4561 aaaatcagag atgaacatga tatatgcctt ggacagtttg aatcagagga attacttgat
     4621 gagattctta aaagagtaat cccagtcata tataatgtgg ctattgatga tgctataaaa
     4681 tcagtaagga atgttgggga tagattagag gaggaattag atatgaggaa ggtagtgtga
     4741 ctaattttaa atcagactta aatctaataa gaaaagaatg gtataatgca ttttattcag
     4801 gtaatactga aatgctagat tacttggaag ctgattggtt tatttcaaca aatggtcaaa
     4861 aagttatcta taaaaaacat cagttacgaa agattgatat gcgtccagcg aggattttac
     4921 ctagcataga aagatatgaa tatgatgtcg taatgcgtga gtttaaaggc attgcttgtg
     4981 tttcagggaa agctgaaata aagcaaaatg atggaaatat attaatggca tttattgaga
     5041 attggataaa ggttaatggt ggatggaaaa tgcagtttat atcgtatgaa aaaatctaag
     5101 gggaagcccc cttagattaa tggttatata tgttcaagtt ttattttttt atagcgataa
     5161 gttctgcttt gagctttaag catgttttct gcaaactgaa tatcaaaact atttatagca
     5221 ttagttatta tagtttttat tttttcttta tcagataatg aatcaccatg ctctataggt
     5281 tcccgcatat atggattgtt ttttaattca tcaaataatg ttttaaatag ttctaatcga
     5341 cgaggtctta taactgtaaa agatatatga agagagtgtt tgctctgagt cgtaacattg
     5401 tgccagtatc cagcaggaag atatatggcc tgacctgggg ttatatcaaa ctcataatct
     5461 ggggaggtat taggtggtag ataatcgaag cttctttcat cttcgagagg gtatgaatag
     5521 gtagggttgt aaactttcca tttttttatt ccctcaattt gaacagctat tacatcatga
     5581 ttgtcaaaat gaagcccaaa gcttggtgtt gctgtaaatg cagcatatag attagcacta
     5641 catgtacatt ctaactcttt agataaccat agtctgcttt catctacaga ttcaaagaat
     5701 gactggcatc gatctactat aagggttgcg ccatcacgta agcatttata tagctgatgg
     5761 cgatttatat gtggtgttct atcgcctgtt tggctatatg tgtatcttat aaatccttta
     5821 taccccctaa tttcgggaaa gttgtcattt gccattctaa ctctaggata gtgcaatata
     5881 tctttttcaa gcaatttatt aagatcattc cagttgatta agtttttata attaacatcc
     5941 gtgaatacaa acggatattt ctggaaatta tatttcttaa atttttctat gatttccttt
     6001 tttataagat tattcatttt taaacctcat gttttgtgat atctataatc tgtgctttag
     6061 gtatattatt tataagttgt tcatcatgag atataacaat gatatgcttt tccttgtttt
     6121 cctcttttat ataatcactg attattttta taccaagtga atcaaaccct tttgtcggtt
     6181 catcgaaaat aatcaagtct acgttttttc cagatgtaag agatactcct aatttttgtt
     6241 tttgtccacc agataaatta tgtccgtaac ctttgatctc ataatctagt ggtttgtcga
     6301 acttaaatct gtgtagcagc tttttaattg tatttaaatt attaatgccg gaataatgta
     6361 atatattttc ctctatagtt ccaggcatga gatctagatc ttgaggaacg tataagacat
     6421 taacaaaagg agaatttgct ttttcttgtt gtataccatt gatttttatt gtgttttctt
     6481 ctgttgttat attgccagag agaatttttg caatagttgt ttttcccgaa ccattctcac
     6541 cggttataaa ggtaacaacg ccttttgtta atattaattt atggttgaat gatttgttgt
     6601 atatttcaat attgcaaata ttgtctttta catgaagata ttgcttgtct gtgtaatttg
     6661 ttttttttat gtcgtttatt tcggcacagt ataaatcaat ttctgttctt gccattttta
     6721 tttgtctaaa tagataacca atgcgattta atgggagaaa aacttggaat aaaaaagtgg
     6781 caagcataat aatactacca cggcttgctc taccatgaag aaacatccag tatgctgata
     6841 ggataatcag actactaaaa atgactgaaa gaaaagcgga gctgatacca aacaggaaaa
     6901 attcattatt tacttttctt tgtttttctg aatgtgtatt tacagcttga attactcgtt
     6961 tacgactttt atccgtggta tggaaagctt tatccatagg atacatgtgc atcatatcaa
     7021 acattcttcc agatgcgaat ttggagctaa gtgcaactga tttcatcatt ggaaatctac
     7081 gttgtgtcat ttttatgaca aatattatat agattgccat tattattata aactcaatgg
     7141 ctattaatgt attaacactt atacaaattg ttatgattat tccaatcatt tgtattaata
     7201 atggtactac atcagaacat attgtataaa ataaagatga tgcagaagaa agcatgcttt
     7261 ctataacctt atttagttct cctttatttt tctttaaaaa atgctcttgg tgagaatttt
     7321 gtatattatc aataacttgc tctctaagct ttacagataa ttctatattt gttttttgag
     7381 tcaagtattc cgatatgcta tatccagcag gcataaggaa tctaattgat aagaaacatg
     7441 ctataaatat taataatgag tttatttctc ctattgaatc tttattgtca ataactgttt
     7501 ttattaaata tccgaataga acgaaaagaa aactacttat agttgataat aatataataa
     7561 aaaacaaatt acatttatta aatgtgatga tgtacttttc aataaaattc aatttataat
     7621 ccttaaagcc cgtatattac gggctttatg ttatctagaa ttacatgagc acccagcgcc
     7681 acatccttta ccgctgcaac cacaaacaga accattatca ttccaaacat cttttcgcat
     7741 tattattttt acaaattctg cggctggaag gtgagtgata ctctttactt tttcgatgta
     7801 agaatcactg gatatggatt cttttggaat tcttatagta ttaatgttct ttatgctgta
     7861 tcttacagca taatcaccat accactttgc gacagtttca tgacctaggt aaaaagcgat
     7921 tttttcaatt tgattttta
//
