LOCUS       NZ_AP022670             1934 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Kluyvera ascorbata strain TP1631 plasmid pKATP5, complete sequence.
ACCESSION   NZ_AP022670
VERSION     NZ_AP022670.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00204448
            Assembly: GCF_015099135.1
KEYWORDS    RefSeq.
SOURCE      Kluyvera ascorbata
  ORGANISM  Kluyvera ascorbata
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Kluyvera.
REFERENCE   1
  AUTHORS   Wajima,T., Hirai,Y., Otake,T., Momose,Y., Nakaminami,H. and
            Noguchi,N.
  TITLE     First isolation of an IMP-1 metallo-beta-lactamase-producing
            Kluyvera ascorbata in Japan
  JOURNAL   J Glob Antimicrob Resist 23, 228-231 (2020)
   PUBMED   33065331
  REMARK    DOI:10.1016/j.jgar.2020.09.026
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Wajima,T. and Noguchi,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2020) Contact:Takeaki Wajima Meijo University,
            Faculty of Pharmacy; 150 Yagotoyama, Tempaku, Nagoya 468-8503, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Coverage                   :: 295x
            Sequencing Technology             :: Illumina Novaseq6000; GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:19:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,171
            CDSs (total)                      :: 5,049
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 43 of 121
            Pseudo Genes (incomplete)         :: 87 of 121
            Pseudo Genes (internal stop)      :: 11 of 121
            Pseudo Genes (multiple problems)  :: 17 of 121
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022670.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Kluyvera ascorbata"
                     /mol_type="genomic DNA"
                     /strain="TP1631"
                     /db_xref="taxon:51288"
                     /plasmid="pKATP5"
     gene            complement(join(1596..1934,1..378))
                     /locus_tag="KATP_RS25780"
     CDS             complement(join(1596..1934,1..378))
                     /locus_tag="KATP_RS25780"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_064761962.1"
                     /translation="MWQARFYQSLPRIVADYPDARWMFLTLTVRNCAIGELGETLNRMN
                     MAFQRLKDRKEFRPVQGWIRTTEVTRGSDGSAHPHFHTLMMVPPSMFTRDYVKHDRWVE
                     LWRECLRVNYDPNVDVRAVKPRKPKDGESLASATAELVRGAVAETLKYSTKPADMVADP
                     EWFLELTKQTHKRRFVATGGALKDILKLDQETDADMVIGDDIPEGDDDGSRIAFEWKTE
                     SKKYRRSPSKDKAESD"
                     /name="protein rep"
     gene            complement(635..937)
                     /locus_tag="KATP_RS25785"
     CDS             complement(635..937)
                     /locus_tag="KATP_RS25785"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     Typing          1679..1870
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.601"
                     /coverage="100.0"
                     /evalue="1.39e-59"
ORIGIN
        1 gcggggtttt actgctcgaa catcaacatt cggatcgtaa ttcacacgca aacactctcg
       61 ccaaagctct acccagcggt catgtttaac gtaatctcgc gtgaacattg agggaggaac
      121 catcatcaga gtgtgaaagt gcgggtgcgc actaccgtca cttccacgtg taacttccgt
      181 ggtgcggatc cagccctgaa caggtcgaaa ctctttgcga tccttaagtc gctggaaggc
      241 catgttcatt cggttaagtg tctcacccaa ttccccgatc gcgcagtttc gcactgtgag
      301 ggtcaggaac atccagcgtg catctggata atccgcaaca atacgcggca gggactgata
      361 aaaacgggct tgccacatca gggatcgcct ccactggcag acaggacagt gccggacacg
      421 gcagaaatgc gcctcacgta gcctaaggcg agtttctcca gtttctttga gggtagacca
      481 accaaacctc agtaaaccgc cacaggaagc catacgcgcc ccgtagcgct catattcggc
      541 tgcgagaagg taaatcccac ctacgtcgtc agaaacgctt ttgtgagcgt cccagggcgc
      601 gtctgagggg gagtaatccg tgaggttctg gagtttaggg ttttgcatgc acaccgtccc
      661 atgctgggcg atggctcatt tgacgtgtta tcattgccgt atcacctgtt ggttgtcgct
      721 tcgaatcgaa tttctgcagg tggtcacacc cccgaacgat ggccgtcgtt gcgggggtcg
      781 cttttaaggc gaccactccc aacagaatac ttccaaaacc ttctacaacg caaataagcc
      841 ccgaagcctt ggtacgcaag gcttgatgcc attttttatc gccagagtta tcccttaagt
      901 ataaatacaa ggatcgcgcg cggatcgaac cagccattca acgcccaccc gccaccaaaa
      961 actgccacaa cgccaggagc gaaacagcaa cgctcaacgc actatcaaca tgattaagaa
     1021 cctcaaaaaa cataacaacc tcactcgaat ggaattgggt aaatctattt agtctgtgat
     1081 ttgcgaaccc atactttttc gcatcacgac agaaccaact ccattcgttc tggttgtatc
     1141 cggtaccaga caattaaaaa ccgtcttcca ggtggttggc ctcacttcgt gaatccaacc
     1201 acctcagcca atttttcaat cagcaatgtc gtcaatcact aaatcggaac ccactggcaa
     1261 gccagcggga ccccacgatt tagagtttga cgatttcgga aacggcgagc gctggcagat
     1321 tttaatctga ctgcgtgagg tgttggcagg gtgtcagggc gcagccctga cgaatcgttt
     1381 ttggcaccga catcgtgatg gcggtgtacc aaaaacacat cttgtcccaa ctgagttcgg
     1441 aaaccgaact cagtaattga gtgacgtcac tttgcggcgt tagccctatc ctgcatcgga
     1501 gtagaaccaa taggacaaga tgtgtttttg gagtaccgcc gacaagcggc gtcctctcaa
     1561 aaacgtcgtg gtcagggctg cgccccgaca ccctgtcagt ctgattcagc tttatctttt
     1621 gacggtgagc gacggtactt tttggattcg gttttccact caaaagcaat gcgtgaacca
     1681 tcatcatcgc cttcgggaat atcatccccg attaccatat cggcatcagt ttcctgatcg
     1741 agtttcagta tgtctttgag tgcgccaccg gtagccacaa atcggcgctt atgtgtctgc
     1801 ttagtcaatt cgaggaacca ttcaggatca gcaaccatgt cagccggctt cgtggaatat
     1861 ttcagggttt cagccactgc gccccgtaca agttccgcag ttgcagaagc caatgattct
     1921 ccgtccttag gctt
//
