LOCUS       NZ_AP022669             4294 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Kluyvera ascorbata strain TP1631 plasmid pKATP4, complete sequence.
ACCESSION   NZ_AP022669
VERSION     NZ_AP022669.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00204448
            Assembly: GCF_015099135.1
KEYWORDS    RefSeq.
SOURCE      Kluyvera ascorbata
  ORGANISM  Kluyvera ascorbata
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Kluyvera.
REFERENCE   1
  AUTHORS   Wajima,T., Hirai,Y., Otake,T., Momose,Y., Nakaminami,H. and
            Noguchi,N.
  TITLE     First isolation of an IMP-1 metallo-beta-lactamase-producing
            Kluyvera ascorbata in Japan
  JOURNAL   J Glob Antimicrob Resist 23, 228-231 (2020)
   PUBMED   33065331
  REMARK    DOI:10.1016/j.jgar.2020.09.026
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Wajima,T. and Noguchi,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2020) Contact:Takeaki Wajima Meijo University,
            Faculty of Pharmacy; 150 Yagotoyama, Tempaku, Nagoya 468-8503, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Coverage                   :: 295x
            Sequencing Technology             :: Illumina Novaseq6000; GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:19:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,171
            CDSs (total)                      :: 5,049
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 43 of 121
            Pseudo Genes (incomplete)         :: 87 of 121
            Pseudo Genes (internal stop)      :: 11 of 121
            Pseudo Genes (multiple problems)  :: 17 of 121
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022669.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Kluyvera ascorbata"
                     /mol_type="genomic DNA"
                     /strain="TP1631"
                     /db_xref="taxon:51288"
                     /plasmid="pKATP4"
     gene            join(4108..4294,1..1100)
                     /locus_tag="KATP_RS25760"
     CDS             join(4108..4294,1..1100)
                     /locus_tag="KATP_RS25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899670.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_239509525.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWKRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTRKGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            1876..2067
                     /locus_tag="KATP_RS25765"
                     /old_locus_tag="KATP_50840"
     CDS             1876..2067
                     /locus_tag="KATP_RS25765"
                     /old_locus_tag="KATP_50840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_163219962.1"
                     /translation="MTKQQKTALNMAKFIQAQSLLLLEKLNELDLDTEADLCEALHEDA
                     EHLFRSLTTRIDGLQEKY"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2067..2474
                     /locus_tag="KATP_RS25770"
                     /old_locus_tag="KATP_50850"
     CDS             2067..2474
                     /locus_tag="KATP_RS25770"
                     /old_locus_tag="KATP_50850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440114.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_035897801.1"
                     /translation="MAQVSISEAARLTGKSRTTLHRLIKAGELSTCHGERNAKMLDTSE
                     LLRVFGALRGLPDEQVVEQVTGQRVTASLSENEQVVRALKQEVEHLKSLVSAQNSHIDS
                     LKQAMQLLEHKQSASDPQKVPAKPWWQFWKG"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3138..3383)
                     /locus_tag="KATP_RS25775"
                     /old_locus_tag="KATP_50870"
     CDS             complement(3138..3383)
                     /locus_tag="KATP_RS25775"
                     /old_locus_tag="KATP_50870"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_163219960.1"
                     /translation="MKKWLTEFFDDMRYIWGEFVREVNSATTDDDTDALDAPGAEYSAE
                     WHATPDFDPQKAINNIDNVYTHDTLHAHIYGHDYDH"
                     /name="hypothetical protein"
     Typing          complement(1142..1641)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.604"
                     /coverage="99.0"
                     /evalue="1.02e-154"
ORIGIN
        1 taagttcacc ggaatacagc cagttctgga agcgccttgc caggggagaa agctttagtg
       61 gcaagtacct gcggctggca aagggaaatc gttccgtatg gcttgaagcc agttatattc
      121 ccgtttctga caggcgcggc cgtgttatca gagtcattaa aattgctgcc gatatttctg
      181 agcgcgtgca ttctgctctt gagcaggaag cagtcgtaaa tgccataaac cgttctatgg
      241 ccatcatcac cttcaatcct gaagggatcg tgcttgaagc aaatgaaaat ttcgtcaatg
      301 ccactggcta taagcgggat gaaatcatag gtaagcatca tcgtctgttc tgtgccgaga
      361 cgctttacaa aagtgatgaa taccgacatt tctgggagag tctgaatcaa ggtgagtttt
      421 tttctggtct gtttccacgc cttaaccgtc agggagaccc tctgtggttc cgtgcaactt
      481 ataaccctgt ctttaacagt gatgggcagc tttataaaat tgtgaaattc gctacagatg
      541 ttacggctga tgtcctgcga aaccagagag agcaagaggc tgctgtgcat gcatgggata
      601 tggccgtgca gacccgagaa agtgcgcaga acggtgccaa tgttattgaa aatagcatcc
      661 ttatgattga cagaattgcg caggggatgg gggctgtctc caccgatatc tcacggctca
      721 acaatcagtc tgaaagtatt gacgatatgg tggaaaccat ccggaagttt gccatgcaga
      781 caagacttat tgcactgaat gccgcgattg aagcagcaag ggctggcgca tccggaagaa
      841 gttttgcggt tgttgccgca gaagtgcgta atcttgcggc gagcgtcagt agcgcgactg
      901 aagaaattga gcaagtcgtg gccagcaata gtcaactggc caaggatgtt ctctgtggca
      961 ttgaaaacag cctgatgaac actcggaaag gggttacact catgcgcgaa gcgggtgaag
     1021 ttatgaccag cattcagagg aatgctgcag gggttgagac tgcggttaag gatgtcgcca
     1081 tttcagttaa agccggatag gccatggtgc atatccccat atctgtcgaa catcacagat
     1141 gatcttcttg agatcctttc cgttgcgtcg taatctcttg ctctgaaaac gaaaaaacca
     1201 cccggcaagg tggctttttc gaaggttcgc aagagttgac gctcttttga accgcggtaa
     1261 ctggcttgga ggagcacagt caccaaaact gtcctttcag tgtagcctta tcgcacccga
     1321 aacttcaaga ctctcctcct ctaaacctgt taccagtggc tgctgccagt ggcgctttgt
     1381 cgtgtttttc cggattggac tcaagttgat agttaccgga taaggcgcag cggtcggact
     1441 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgccttcccg gaaccgagtg
     1501 tcaggcgtgg aatgagataa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
     1561 gcaggaacag gagagcgcac gagggagccg ccagggggaa acgcctggta tctttatagt
     1621 cctgtcgggt ttcgccacca ctgatttgaa cgtcatattt tgtgatgctc gtcagggggg
     1681 cgaagcctat gaaaaaacgc ccggatgggc gtgagcttat cgtccttgtc ctgttttttg
     1741 gttacgcttg tcgtctatct tacttgtcac tttgaaagac cggtccgctc gccgcagaca
     1801 agtgaccgag cgtagcgagc gaacggtcga ggaagcggaa aagaaccgga tataacctgg
     1861 agcactgacg cagtgatgac taaacaacag aaaacagcac tgaatatggc gaaattcatt
     1921 caggcgcaat ctctgctttt gctcgagaag ctcaacgaac tggatctcga taccgaggcc
     1981 gacttgtgcg aagcattaca tgaggatgcg gaacaccttt ttcgtagcct cacgacacgg
     2041 attgatggtc ttcaggagaa gtactaatgg ctcaggtatc tatcagtgaa gcagctcgtc
     2101 ttaccgggaa aagtcgtacc acgctgcaca ggttgattaa agcaggtgag ttgtccacct
     2161 gtcacggcga acgaaatgca aaaatgttag atacatcaga gttattgcgc gtatttggtg
     2221 cgttacgtgg tcttcctgat gaacaggttg ttgaacaggt cactggacag cgtgttacag
     2281 catcattgtc tgagaatgaa caggtggtca gggcgttgaa acaggaagtt gaacacctga
     2341 aatcgctggt ttctgctcag aattctcata tcgatagtct gaaacaggcg atgcagctcc
     2401 tggagcataa acagagcgct tctgacccgc agaaggttcc tgcaaagcca tggtggcagt
     2461 tctggaaggg ctgattaccc ctgaaattct gcctttccag tacgattttt tgcatgcaaa
     2521 aaatctgttt atcctgtcag ggcggcgccc cgacacccgc cagcgcctca ccgccacgca
     2581 ataaattgcg tgcccgttca ccgcttatgt ttcctgtctg atcgataagt cttctggtgg
     2641 ctgaaaacga ccagaaaatc gtgtcacacc tgacgcacta tacagtactg ctccgaaagc
     2701 cttgtgctgc aaggctttcg ggcgctacac ccccactgtc tgaagtgtag cgaaaagccc
     2761 ctaaaaacgc agttatctca ttgttttata tggaataaaa agtgtagcga tgagcgctac
     2821 actttttatc ttaaacttcg ctttaattgc atgttttata atgtttttct ttgagtttgg
     2881 ggtgtagcgc ggatgcagtt tatttggatg gatgcttgtt tcaggtctgc ggctttttaa
     2941 gcgtaaattc tggatgtgat ccgggctatt ttttcgcggg gtgtcctgtt cccgagtccg
     3001 aaggctttat cccctcgatg aatcgcgctt gatcttctat tacatcgggt cgctcgacca
     3061 ggaagtcggg ggatgctttt ttcgtgctgg ccaggtcgcg ccacaggact cttttcctgg
     3121 gtgcttttgc atgatgttca gtggtcatag tcgtggccat agatgtgcgc gtgaagggta
     3181 tcgtgggtat acacgttatc aatgttgttt atcgcctttt gcggatcaaa atccggcgtt
     3241 gcgtgccact ctgccgagta ttccgcaccg ggcgcatcca gagcatccgt atcgtcatct
     3301 gtcgtggcgc tgtttacttc gcggacgaat tcaccccaga tataacgcat atcgtcgaaa
     3361 aattcggtga gccacttttt catatcgtgt cctggttgcg ctgtgtgtac atcaacgagt
     3421 gtagctaccg ggagaagata caccggaaag ggataaaaat gcctgattga tggaattttc
     3481 cgtccgtaac gtggtgtcag tcattggcgt gcataacgat tcacgcctgt aaaacgcacc
     3541 agcgcgattc tggggcgttt tcaggaggga agatatgtgg ttttgcggct tcggcaggaa
     3601 aatcgcgtac agcccgtctg agcggtgcgg gcaaggggtg ttatgttaaa agccgaggtc
     3661 tggcggatac ggaaaaaatt atgccggccg ggcttatgcc cgcggcaatc cggccgatac
     3721 ttaaattatc cttcacaaag gatgtgctgc gatccaggca gttgagtgtt ttccggctcc
     3781 cggatgggag ccattttttt gccttctgat gcctgttaat gaatgcggag ctgcgctggt
     3841 tcttccaaga ttgatagacg ccagcttctc tgctggagcc ggcggatttg ccgtaaatat
     3901 gaacattttc ttattgttca gcccgctaaa gatggcgggt tttttacgcg tgaaatccgg
     3961 agccggacat catgccggca tggctgaaga gcgggctttt tcccgccctg tcgcagtaat
     4021 atataaagcc acataaggag tagccgataa ccatggcagt acttacatta tccgggcaat
     4081 ctgattcata tgttttcact taaaaatatg ttctccctga acctccagcg atcagccatt
     4141 tcgtatgacg caatttgtca gaataagcct gtcattgagt tcagtccgga gggggtgata
     4201 aataaagcca gcccgctttt tctctccact atggggtaca gggctgatga aataatcggt
     4261 caccaccatc gtatattctg cccgccctcg ctgg
//
