LOCUS       NZ_AP022668             4666 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Kluyvera ascorbata strain TP1631 plasmid pKATP3, complete sequence.
ACCESSION   NZ_AP022668
VERSION     NZ_AP022668.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00204448
            Assembly: GCF_015099135.1
KEYWORDS    RefSeq.
SOURCE      Kluyvera ascorbata
  ORGANISM  Kluyvera ascorbata
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Kluyvera.
REFERENCE   1
  AUTHORS   Wajima,T., Hirai,Y., Otake,T., Momose,Y., Nakaminami,H. and
            Noguchi,N.
  TITLE     First isolation of an IMP-1 metallo-beta-lactamase-producing
            Kluyvera ascorbata in Japan
  JOURNAL   J Glob Antimicrob Resist 23, 228-231 (2020)
   PUBMED   33065331
  REMARK    DOI:10.1016/j.jgar.2020.09.026
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Wajima,T. and Noguchi,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2020) Contact:Takeaki Wajima Meijo University,
            Faculty of Pharmacy; 150 Yagotoyama, Tempaku, Nagoya 468-8503, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Coverage                   :: 295x
            Sequencing Technology             :: Illumina Novaseq6000; GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:19:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,171
            CDSs (total)                      :: 5,049
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 43 of 121
            Pseudo Genes (incomplete)         :: 87 of 121
            Pseudo Genes (internal stop)      :: 11 of 121
            Pseudo Genes (multiple problems)  :: 17 of 121
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022668.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Kluyvera ascorbata"
                     /mol_type="genomic DNA"
                     /strain="TP1631"
                     /db_xref="taxon:51288"
                     /plasmid="pKATP3"
     gene            155..640
                     /locus_tag="KATP_RS25730"
                     /old_locus_tag="KATP_50810"
     CDS             155..640
                     /locus_tag="KATP_RS25730"
                     /old_locus_tag="KATP_50810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_045326458.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            644..859
                     /locus_tag="KATP_RS25735"
                     /old_locus_tag="KATP_50820"
     CDS             644..859
                     /locus_tag="KATP_RS25735"
                     /old_locus_tag="KATP_50820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1205..1969
                     /locus_tag="KATP_RS25740"
     CDS             1205..1969
                     /locus_tag="KATP_RS25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="WP_045619337.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGEFFFRGIDIFWVMLI"
                     /name="cache domain-containing protein"
     gene            1966..2760
                     /locus_tag="KATP_RS26015"
     CDS             1966..2760
                     /locus_tag="KATP_RS26015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_048961857.1"
                     /translation="MKNTAWNVLVYSHADSATTILITYAKSVLVLLIVIIITFSLLSYF
                     IASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRLKKALFTHVKVMMERVDSL
                     NEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFFKKINDSFGHGVGDEVLVS
                     LAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAERIRSVIERTVFPNNLRVTV
                     SAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSAI"
                     /name="GGDEF domain-containing protein"
     gene            3820..4143
                     /locus_tag="KATP_RS25750"
                     /old_locus_tag="KATP_50830"
     CDS             3820..4143
                     /locus_tag="KATP_RS25750"
                     /old_locus_tag="KATP_50830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4325..4570)
                     /locus_tag="KATP_RS26020"
     CDS             complement(4325..4570)
                     /locus_tag="KATP_RS26020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052686166.1"
                     /translation="MQPQSAVHDGLPGIDAWTVGAIVIRLEPSAGQQLRVRDQKIQLKP
                     PLVTMLHPQDAVLVFIKSGHQNPLKACHQLFPLAGG"
                     /name="hypothetical protein"
     Typing          3535..3707
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.126"
                     /coverage="100.0"
                     /evalue="1.99e-77"
     Typing          complement(3058..3610)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.639"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          2..234
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.436"
                     /coverage="92.0"
                     /evalue="6.36e-39"
     Typing          3821..4137
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.47e-61"
     Typing          4134..4666
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.78e-108"
ORIGIN
        1 cttgctcttg cctcatccgg ggagctgaaa acccgtcagg atgtcactga ggcgctggaa
       61 agcgcaggtt ttgaggtggt ccgcaccaca aaaagcagta tcagcattgc cgacccggac
      121 ggggggcgaa acatccgact gaagggagcc atttatgaac agtcttttaa cgctggcgaa
      181 ggacttagag cagaaatcga aagcgcagca gcagagtacc ggcgagatgc tgaaagccgc
      241 attcagcgag cacgagaaat ctgtaaaagc ggaactgaac gaaagcgcga agagaatcag
      301 cgccgccatc ctcgaccacg accggaaact gtcctcagcc atgagccagc gcacgaaagg
      361 catggtgcgc atggtcagcc agacgtggct gaccatcgtc ctggtctccg cgctgctgat
      421 cgcctcgagc gcgggcattc tgtggtggca ggggcagcag atactcgaca actacacgac
      481 catccgggag cagaagagca cgcaggccat gctgtcagag aggaacagcg gcgtgcagct
      541 ctcgacctgc ggcgagcaga gacgccgctg cgtgaaggtg aacccggaag cgggacggtt
      601 cggagaggat tcgagctgga tgatactggc ggggaaatag cacatgacgg agctggaaaa
      661 acagttgctg agcgcattag agcagctaca gcaggactac tcgaaaaggc tggacgagtg
      721 ggagagcgcc ttcgcggaat ggcggacgat gtctggtctt atgcaacggg agaacgcagc
      781 gctgagcgag cgcgtcacgg gcttgagcag gcaggtgcag agtttgagcg agcagctgcg
      841 ccggttgtcg agcgactgaa tgccgtcgag ctgcaccggg agcaggagcg cgtggtgcag
      901 catcagaaag cgctggagct ggagcgatct cagcgacagc ggacgtataa cagcccttca
      961 ctgtgataac ctgataaaac ggggctttcg gtggacatct gcgctgaaag ccggagccat
     1021 tcacccgact gcatagccat gcagccgggt gaaatttatc tggcgcagaa tgcgcggttt
     1081 agcggagagt ttgctgccgg ttttaccggg ctgacgccgc atttgttccg aacccgtccg
     1141 gggcggtgct gataagggat gttatggtaa atgcggcatt ttttattata tggagtgtat
     1201 ttttatgaga attaaaaagc ctcaccttcg tccgatagtc ataaccttgt ccgttggtgg
     1261 gattttgtta acttctattt ttctgattgc cgttattatg gtttttcagc gcgaaaatat
     1321 agaaaaagat ttgcttgatg ctaattcatc gtacgctatg aaaatgtcag atgtaatgag
     1381 tggttatatt gaaatggctc aaggtgaatt agcatatgga gcaaaaaaaa taaaatcaac
     1441 taatgatatt gactcactta aagatgaggc ggataggtta agattgcagt ctggtatgtt
     1501 taattcagtt tttgttgtta gtaatgatgc tgttgttctg gcgacttcgc cagaatctct
     1561 tgatttagtt ggtacccgct tgaattcaag tgccagcaaa ttggcgattg aggaaaagaa
     1621 accattcata tcttcaccgt tcaaatcagt tactgggaat ttgattattt tgttgtcaca
     1681 tcctatttat gataagaatg gcaattacat agggcatgtc ggtgggtcta tatatctaaa
     1741 aaaacatagt ttatttagcg atgttttgag caggcatttt ttcaagagcg acactgaaat
     1801 tagcattgtt agtgatgatg gaactgtgat ctttaataac gataacagtg ctgttggtcg
     1861 gcccatggtt atgtctgatg atttgaaatc aaagttaacg gagtctgata gaggtgaggg
     1921 ggaatttttt tttcggggaa tagatatctt ctgggttatg ctcatatgaa aaatactgct
     1981 tggaatgtgt tggtttatag ccatgctgac agcgcgacta ctattcttat tacttatgct
     2041 aaaagtgtac ttgttctgct aattgtaata attattacat tttcacttct ttcatatttc
     2101 attgcctccc gtatttcact tcctttagaa aagcttgcta tatctaccaa tgctaaagat
     2161 ataggggctt cgttagttta tattaaagct atcaatgctt ggtatgccga agctgatcga
     2221 cttaaaaagg cactttttac gcatgttaag gtaatgatgg aaagggttga ttctttaaat
     2281 gaggtggcgc ttaaagatcc ccttaccggc gtgaacaata gattggggtt ttcaactaaa
     2341 atacagaata acaagtatgg ggcaggagct tcagttattg ctattgatgt tgattttttt
     2401 aaaaaaataa atgatagttt cgggcatggg gtcggagatg aggtgcttgt ttctttggct
     2461 cagattatta agtcttgttg ccgtagtgaa gatattattt gtcggtttgg tggagaagag
     2521 tttgttgttt ttctgccgaa tacatctgtg gtaacagcgg agtgtgttgc ggagcgtatt
     2581 aggagtgtta ttgaacgtac tgttttccct aataacttgc gtgtcaccgt atctgctggt
     2641 gtggcaacac aagatgatcc tcttggtggg atcgattttt tgctgaaaaa tgctgacgat
     2701 gctctttatc aagctaagag agaggggcgt aataaagtga ttgtatatag cgctatttga
     2761 gtttttttga tgacgcttta cgcgctagtg tgccgttttt ttttgtgggg aggggggttc
     2821 aagaagcgga tcctcttgtt atcccttcga tgtcgtcgta atctcatgcc ctgcaaacaa
     2881 aaaaaccacc gctaccaacg gtggttcgtt tgccggaatc aagagctacc aactctttct
     2941 tccgaaggta actggctttc acggagcgca ggtaccaaaa ctgtccttcc agtgtagccg
     3001 cagtcaggcc accacttcaa gaactctgta gaacactcta ctactccgct ccgtgaatcc
     3061 tcttactaat ggctaccgcc actggcgatt tttcgtgtct gttccgggtt ggactcaaga
     3121 tgatagttac cgggacaacg tggtagtcgg gctgaacggg gggttcgtgc atacagtcca
     3181 gcttggagcg aactgccttc ccggaaccga gtgtcaggcg tggaatgaga taaacgcggc
     3241 cataacagcg gattgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag
     3301 ccaccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt
     3361 gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggcttcgcc
     3421 gtggccttat cgcttccctg ttaagcttct tcctggcatc tcccaggaaa tttccgcccc
     3481 gcctgtaagc cggtaccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3541 aggaagcgga atatatcttg tatcacatat tctgctgacg ctccgctgct gcatttttct
     3601 cctgccacat gaagcatttc actgacactc gcatccgtgc caacatagta agccagtata
     3661 cactccgcta gcgctacgtg actggttcag ggctgcgccc cgaaacccgc caaaagcctg
     3721 acgcgcctgc ggcttgtcca accccgcgcc gaccgggaaa ggctttcccg cccgtcccgg
     3781 cgttatcagg aggccggttt gtcagagaaa cggaacaaga tgctcaccat gtgggtgacg
     3841 gaggacgagc accggcggct gcttgagcgc tgcgacggca ggcagctggc agcgtggatg
     3901 cggcagacct gcctggacga aaagccggca cgttccggaa agcttccctc aatctcgccg
     3961 gcgctgctgc gccagcttgc cggcatgggc aataacctga accagatagc ccgccgtgtt
     4021 aacgccggcg gcggtaccgg ccatgacagg gtgcagattg ttgcggcgct gatggccatc
     4081 gatgccggac ttgagcggct gcggcacgcc gttcttgaga aaggaacgga cgatgatcgt
     4141 taaatttcat ccacgcggtc gcggcggtgg cgccgggccg gtggactatc tgctgggtaa
     4201 agaccggcag cgtgaaggcg ccaccgtcct gcaggggaag ccggaagaag tccgggagct
     4261 gattgacgcg tcgccttacg ttaagaaata cacctccggg gtactgtcgt ttgcggaagc
     4321 ggatttaccc tccggccagc gggaaaagct gatggcaagc tttgagcggg ttctgatgcc
     4381 cggacttgat aaagaccagt acagcatcct gtgggtggag catcgtgaca aggggcggct
     4441 tgagctgaat tttctgatcc cgaacacgga gctgctgacc ggcagacggc tccagccgta
     4501 ttacgatcgc gccgaccgtc cacgcatcga tgcctggcag accatcgtga acggcagact
     4561 ggggctgcac gacccgaacg caccggagaa ccggcgggca ctggtcacac cgtctgcact
     4621 gcctaaagcg aagcaggagg ctgcagaagc gattacacgc ggtctg
//
