LOCUS       NZ_AP022657             2113 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli strain 09-02E plasmid p7-09-02E, complete sequence.
ACCESSION   NZ_AP022657
VERSION     NZ_AP022657.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00198301
            Assembly: GCF_012149945.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Mohsin,M., Tanaka,K., Kawahara,R., Kondo,S., Noguchi,H., Motooka,D.,
            Nakamura,S., Khong,D.T., Nguyen,T.N., Tran,H.T. and Yamamoto,Y.
  TITLE     Whole-genome sequencing and comparative analysis of the genomes of
            Bacteroides thetaiotaomicron and Escherichia coli isolated from a
            healthy resident in Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Mohsin,M., Tanaka,K. and Yamamoto,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2020) Contact:Mashkoor Mohsin Institute of
            Microbiology; UAF main road 38000, Faisalabad, Punjab 38000,
            Pakistan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Platanus v. 1.2.4; Canu v. 1.7
            Genome Coverage                   :: 143X
            Sequencing Technology             :: illumina Miseq, Minion
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 02:15:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,204
            CDSs (total)                      :: 5,083
            Genes (coding)                    :: 4,862
            CDSs (with protein)               :: 4,862
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 81 of 221
            Pseudo Genes (incomplete)         :: 153 of 221
            Pseudo Genes (internal stop)      :: 36 of 221
            Pseudo Genes (multiple problems)  :: 46 of 221
            Pseudo Genes (short protein)      :: 4 of 221
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022657.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="09-02E"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p7-09-02E"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /lat_lon="20.445128 N 106.337870 E"
                     /collection_date="2018-10-10"
     gene            join(1797..2113,1..649)
                     /locus_tag="HRS14_RS25675"
     CDS             join(1797..2113,1..649)
                     /locus_tag="HRS14_RS25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            653..919
                     /locus_tag="HRS14_RS25680"
                     /old_locus_tag="Esc0902E_49860"
     CDS             653..919
                     /locus_tag="HRS14_RS25680"
                     /old_locus_tag="Esc0902E_49860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          78..309
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gtcttcccga tggttgtttc tgacgttaac tgtcaggaac tgcgagatag gtgaacttgg
       61 aacagtcctt acagcaatga atgcggcgtt taagcgaatg gaaaagcgaa aggagctatc
      121 acctgttcag gggtggatca gggctacgga ggtgacgcga ggtaaggatg gcagcgcaca
      181 tccgcatttt cactgtctgc tgatggtgca accttcttgg tttaaaggga agaactacgt
      241 taagcacgaa cgttgggtag aactctggcg cgattgcttg cgggtgaact atgagccgaa
      301 tatcgatatt cgggcagtaa aaactaagac aggtgaggtt gtggccaacg ttgccgagca
      361 actgcaaagc gcggttgctg aaacgctgaa atactccgtt aaaccggaag atatggcaaa
      421 cgatcctgag tggtttcttg agctgacgcg gcagcttcac aagcgccgtt ttatctcgac
      481 cggtggggcg ctaaaaaacg tcctccagtt ggatcgagaa accaatgagg atcttgtcat
      541 tgccgacgat gtaggggatg gcactgatga cgggaagcgg acggcgtttg tctgggattc
      601 aggtaaacgg cgttacaaac gcgcccctga gaaggataaa tcggattaac gtatgaatat
      661 taatattgaa tacctgaatg gaaataagac tattggttta ttttttttaa gaagtgaagc
      721 ggtgattcct gacaggttta aaaaccttat tttgcttatt gatggattaa gttttggcac
      781 atttggtttt catccgcacg aaggttttga ggatgaatta attttatata ttcagaaaac
      841 aaacgagagg gtaaaaactc tttttgtgaa aatagattta aacggtatca agtttggatt
      901 tttaagaacg cattcttagt tctggaaaag agccagcggc aggctgaggt gataggtacg
      961 agattgcatg caatctctag tgctctgtct atcctgcatt atcctcagca ttatcctcag
     1021 cattatcctc agcattatcc tcagccttgc caactcgaca ccaatgcagg atagacaatc
     1081 cgatgtcaaa tgttaacact ctgcgagtgg tacattttcc ccggattatc gtcctgagcc
     1141 tgccgctggc tctctttcta ccgcctcgct ttgctcgttg ctcaacgcct cacagacacg
     1201 gattaaaatc cgcatccgtt caccgttttt taaagtccgt taaaagcatg atgccatctc
     1261 cgagagttaa tctcgtcaaa tgctaaatcg tgggggtccc ctttggggtt ccgatttagt
     1321 gattgacgac accaccgatt aaaaaactta tgcggggtgg atggtttcac gaagtgaggc
     1381 catccacctg taagacaggg ttttgttttt attccctgtt ttggtgatcg ggtgtgtgga
     1441 aaaggttggg gtaagccgtt cgggggtgct tgttttgggg ggttaaaatt gtggttattt
     1501 tttgcgcaat tctcgcgcgt gatccttgta tttatactta agggataaat ggcggatatg
     1561 aaatagtggt ttagcccagt aatgacgagg ctttgagtgg gttttgacag gtcaaagaaa
     1621 atggagcaga attgaggcgt ttttaatcgg cgttggggag tgcgtcaaca ctccccaaca
     1681 tttcgaatgt gtcacctcag cggcaaactc tggtgacatg tactggctcg caatgcacag
     1741 gtacgtgatg aatataccac atcaaatcac agcctgccca gatcggagca ggcttaatgt
     1801 cagaagataa attcctttcg gactacagcc cccgtgatgc agtttgggat acccagcgca
     1861 cgcttaccga ttctgtcggg ggtatctacc agactgctgc tgaattcgag cgctatgcac
     1921 tccgtatggc ctcctgtagc ggtttgttac gttttggttg gtctaccatc atggaaaccg
     1981 gagaaacgcg cctacggctt cgtagtgcgc aattttgccg tgtccgtcat tgccctgtct
     2041 gccagtggag aagaaccctc atgtggcaag cccgttttta tcaggctcta ccgaaaatcg
     2101 ttgtggatta ccc
//
