LOCUS       NZ_AP022656             2739 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli strain 09-02E plasmid p6-09-02E, complete sequence.
ACCESSION   NZ_AP022656
VERSION     NZ_AP022656.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00198301
            Assembly: GCF_012149945.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Mohsin,M., Tanaka,K., Kawahara,R., Kondo,S., Noguchi,H., Motooka,D.,
            Nakamura,S., Khong,D.T., Nguyen,T.N., Tran,H.T. and Yamamoto,Y.
  TITLE     Whole-genome sequencing and comparative analysis of the genomes of
            Bacteroides thetaiotaomicron and Escherichia coli isolated from a
            healthy resident in Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Mohsin,M., Tanaka,K. and Yamamoto,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2020) Contact:Mashkoor Mohsin Institute of
            Microbiology; UAF main road 38000, Faisalabad, Punjab 38000,
            Pakistan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Platanus v. 1.2.4; Canu v. 1.7
            Genome Coverage                   :: 143X
            Sequencing Technology             :: illumina Miseq, Minion
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 02:15:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,204
            CDSs (total)                      :: 5,083
            Genes (coding)                    :: 4,862
            CDSs (with protein)               :: 4,862
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 81 of 221
            Pseudo Genes (incomplete)         :: 153 of 221
            Pseudo Genes (internal stop)      :: 36 of 221
            Pseudo Genes (multiple problems)  :: 46 of 221
            Pseudo Genes (short protein)      :: 4 of 221
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022656.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="09-02E"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p6-09-02E"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /lat_lon="20.445128 N 106.337870 E"
                     /collection_date="2018-10-10"
     gene            complement(567..1565)
                     /locus_tag="HRS14_RS25670"
                     /old_locus_tag="Esc0902E_49830"
     CDS             complement(567..1565)
                     /locus_tag="HRS14_RS25670"
                     /old_locus_tag="Esc0902E_49830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001672436.1"
                     /translation="MKNPELKNLTDYSPADKPWDEHRSFSDDVGGIYLRAAGFEHYGER
                     VGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPKIV
                     TEHPGARWLFLTLTVRNCSIDALGETLTAMNAGFKRLKERKEFRHVLGWVRTTEVTRGK
                     DGTAHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRKPKDD
                     ESLPCVVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGALKNVLQ
                     LERETNEDLIMADDMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRQLSGED"
                     /name="protein rep"
     Typing          complement(2596..2675)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.062"
                     /coverage="88.0"
                     /evalue="9.44e-28"
     Typing          659..850
                     /name="Col(MP18)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.482"
                     /coverage="100.0"
                     /evalue="1.32e-94"
ORIGIN
        1 ggggatggta ttagcgccgg gatgcgacag aaacgcgctt aaaacgcccc tgaacgcatc
       61 aaacgcgaaa aaacgaacaa aaccttgcct gcctgcaaaa atcacgttag aacgcgtctg
      121 agcgcgtctg aggggttaaa attcactgtg cgtcaaatgg ggattattac accccgccct
      181 caaatgcgct tacttcccct tcctgtccat gtgcggaaat gttgttgctt tgtatctttt
      241 gtgttaagca acggatgagc gtgtttcttt cgcatttcca ctatcatcaa cccacgaaga
      301 cctgtagaaa cacgctcccc ccctccgact actggacagc agaatcacac cgcgctaaac
      361 atgacatgcg tgtttctggt gagcgaaaag ctgtcagccg tggactgccg tagcagagac
      421 ccggaaacgc gcttaaaacg cttctgagtg cgtcaaaaca aaaaacgagt aaatccttgc
      481 tcgtttctga aaatcacgtt agaacgcttc tgagcgcgtt tctgaatatt ctcccagtgc
      541 atgaatagtt cgcgcctgaa acgcgctcaa tcctcgccac ttagctgacg tttatctttc
      601 gcccgatcgc gctggtattt tttgacaacc ggcacccacg aaaacgccag acgggaaccg
      661 tcatctgtac cctctgccat gtcatccgcc atgattaaat cctcgttcgt ttcccgctcc
      721 agttgcagaa cgttcttaag cgctcccccc gtagcaacaa aacgacgctt gtgtacctgc
      781 cgtgttaatt cgaggaacca ttcaggatca gccagcatat cagcaggctt tgtggaatat
      841 ttcagagttt caaccaccgc gccgcgcagc tgttccgcaa caacacaagg taaagattca
      901 tcgtcttttg gtttccgtga tttcaccact ctgatatcca cattcgggtc atacgccacc
      961 cgcagacaat cacgccacag ttccacccaa cgggcatgtt tcacatactc acggccggac
     1021 aacatcgaag ggggaaccat cagcagagtg tgaaaatggg gatgtgcagt accatccttg
     1081 ccgcgcgtta cttccgtggt acgaacccaa cccaacacat ggcgaaattc cttacgctct
     1141 ttcaaccgct taaaccccgc attcatcgcg gtcagcgtct caccaagcgc gtcaatggaa
     1201 cagttgcgca ctgtcagtgt gagaaacaac cagcgcgcgc cgggatgttc cgtcacaatc
     1261 tttggcaggg actgatagaa acgggcttgc cacatcatgg aacgcctcca ctgacagatc
     1321 ggacagtgcc ggacgcgaca gaaccgtgca tcacgcaacc gcaggcgcgt ttctccggtc
     1381 tctgtgtttg ttgaccaccc aaaccgcaaa ataccactac aagccccgac acgttcgccg
     1441 tagtgctcaa atccggcagc acgcagataa ataccgccca catcgtcgga aaagcttcgg
     1501 tgttcatccc acggtttatc tgccggagag taatccgtca gattcttaag ttcaggattt
     1561 ttcatgtctg ccgtcccctt gcgagcgaca gacgaaattg cgtgttatca ttcctgtatc
     1621 acctgtacca attgtcgtca aatcagttca tgttctaggt ggtcgcgccc ctggagtggt
     1681 ggaagactcg caggggctaa tttataacca ctcccgacaa aatactctca aacctattta
     1741 cacgcaaatc cgcgctcagg ccagatatgg caagggatag agctgattca aaaaatcgca
     1801 tttatccctt aagtataaat acaaggaacg cgcacgaaag cagcagcaaa atcgcctgtt
     1861 ttttgttcga ttttttgagg gcaaaaacaa caaccacgaa agcgttttcc cgacaaaaca
     1921 cccacaggct tttccaccaa ttttgtgaac aacaaaaacc gtcttgcagg tggttggcat
     1981 cactccgtga caccaaccac ctcctccagt gttttttccg tgccatcgtc aatcacaaaa
     2041 tcggaaccca cgccggaggc gcgggacccc acgattttct gtttgacgaa ttcgggaacg
     2101 gtgagcagta cgcggaattt tttcacgtgc cgcgaagcgt tggcagggtg tcggggcgta
     2161 gccctgacca ggtggggaat gtctgagtgc gcgtgcgcgg tccgacattc ccacatcctg
     2221 tcccgatttc tgcgagtttg tgttaccgga acgctggcgc aatatcggca gtttttgcgc
     2281 cgtgtcgccc tccacggaag cgcgcagatc tggcaaccgg acggcaaatc attttgccgt
     2341 tttcgtgccg cagccgttcc gcgcgatcga caaactggca gtcgggggga atctcacgaa
     2401 gtgcgcactt atacaatctc aacttcgttt cgattgtgtg cggtctgcga cgcggtgaaa
     2461 accgcagata acaggcaaaa aaaatccccc tgtgtcaggg ggccgtcaaa gtcaatttcc
     2521 tgatgatagt tgttttgagg tgttgctatg cccgctcaat ttgccgcagg aacaccgcaa
     2581 aaacaactcc gatgacggga caggatgtgt aatcgtataa ccgcgcacgc acgccgatac
     2641 gattaccacc tggtcagggc tacgccccga caccctcgac aacctgcccg ggacacgtta
     2701 aaacgacatc tgcgcgtttc tgaagctccc attaaccat
//
