LOCUS       NZ_AP022518             2821 bp    DNA     circular CON 30-JAN-2024
DEFINITION  Citrobacter portucalensis strain STN0717-72 plasmid pSTN0717-72-5,
            complete sequence.
ACCESSION   NZ_AP022518
VERSION     NZ_AP022518.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195990
            Sequence Read Archive: DRR199795
            Assembly: GCF_015138175.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter portucalensis
  ORGANISM  Citrobacter portucalensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Citrobacter portucalensis str.
            STN0717-72 isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 877x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 09:07:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,251
            CDSs (total)                      :: 5,137
            Genes (coding)                    :: 4,941
            CDSs (with protein)               :: 4,941
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 35 of 196
            Pseudo Genes (incomplete)         :: 147 of 196
            Pseudo Genes (internal stop)      :: 33 of 196
            Pseudo Genes (multiple problems)  :: 17 of 196
            Pseudo Genes (short protein)      :: 1 of 196
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022518.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Citrobacter portucalensis"
                     /mol_type="genomic DNA"
                     /strain="STN0717-72"
                     /isolation_source="sewage"
                     /db_xref="taxon:1639133"
                     /plasmid="pSTN0717-72-5"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-07-17"
     gene            101..835
                     /locus_tag="JLF48_RS26155"
                     /old_locus_tag="STN0717CIT72_P50010"
     CDS             101..835
                     /locus_tag="JLF48_RS26155"
                     /old_locus_tag="STN0717CIT72_P50010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065807857.1"
                     /translation="MTIQEVKKTRVRTKTENDKLTEYKMNPFVFDKELKIETKTRNLTV
                     RKGTELVNKDNADESESYFSNIVQKKEVDKEEFIKLFTSQIKVYFDLTKTAHKIFLIIL
                     ALYQKEIGKDYVLLTCKKAQNVAKTLDFELSAPIFYRGIKELVEKKIIAKSVDKIVFYI
                     NPAVFFNGDRARFVTEVIKKKEEIEKKQFLEELETTSKNKDFETDDSVENLDLMIARLQ
                     KKKEELERKEKFGQWQDLEDAS"
                     /name="hypothetical protein"
     gene            complement(853..1959)
                     /gene="mobV"
                     /locus_tag="JLF48_RS26160"
                     /old_locus_tag="STN0717CIT72_P50020"
     CDS             complement(853..1959)
                     /gene="mobV"
                     /locus_tag="JLF48_RS26160"
                     /old_locus_tag="STN0717CIT72_P50020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791318.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_085856038.1"
                     /translation="MYSIIRKQSHKKTAIQAVHNHNMRIVIEENVNSKKSNLNEILIGT
                     ENTRSDIMSYIKNNDIVIRNKDTVIFNEFVLTASPEFFFNNKDGSKKTRSEYNDNLKDW
                     VKTQIEFLEKENYGKCVNAVLHLDESTPHIHALILPIVDNKLNNKSFWRGKNSYGRLQD
                     IYNASNSKFGLKRGEEKSKTLVDHTTLKDYRELIKQDLEEEKEFYNELSKDILDIPPRK
                     NLIGLEKKYSAEEVKEIATATFKKLNRQRRRAKFRAKKSDEKADAATSEFYKKKKETSK
                     IEFFYNQNMQQLEKTSVENSTLKTQNLELKQSQSDLDLVRKFLPMELQSLVIRARELAS
                     GGDSGGTKHTAEPTPTADLTTYKRPKYK"
                     /name="mobV"
ORIGIN
        1 gtcgcaaaaa acgatttttt tttatcgttt ccacttgact tcttaatgag atcagttata
       61 aaactagcat taagtatcta aatttaaaga ggaatagatt atgacaatac aagaagtaaa
      121 aaaaacaaga gttagaacaa aaacagaaaa tgataaatta acagaatata agatgaatcc
      181 ttttgttttt gacaaagaac taaaaattga aacaaaaaca agaaatttaa ctgttagaaa
      241 aggtactgaa ttagtaaata aagataatgc tgacgaatca gaatcttatt tttctaatat
      301 tgttcaaaag aaagaagtag ataaagaaga atttataaaa ttatttacat ctcaaataaa
      361 agtatatttt gatctaacaa aaacagctca taaaattttc cttataattt tagctttata
      421 tcaaaaagaa atcggtaaag attatgtttt attgacatgt aagaaagctc agaatgttgc
      481 taaaacatta gattttgagc tttcagcacc aattttttat cgaggtataa aagaactggt
      541 agagaaaaaa attattgcta aatctgtaga taaaattgtt ttttatataa accctgcagt
      601 tttttttaat ggtgatagag caagatttgt aactgaagtt atcaagaaaa aagaagagat
      661 agagaaaaaa caatttctcg aagagttaga aactacatca aaaaataaag attttgaaac
      721 tgatgatagt gttgaaaatt tagatctgat gattgcaaga ttacaaaaga aaaaagaaga
      781 attagagcga aaagaaaaat tcgggcaatg gcaggatctg gaagatgcat cataaaagcc
      841 ctgatgggct ttttatttgt atttaggtct tttataggta gttagatctg cagttggtgt
      901 aggttctgca gtatgctttg taccgccaga atcgccgcca gaggccagtt ctctggctct
      961 gatgacaagg ctttgcagct ccataggtaa aaattttctc accagatcta ggtcactttg
     1021 tgattgctta agttccaagt tctgggtttt aagtgtggaa ttttccacag aggttttttc
     1081 caattgctgc atattttgat tataaaaaaa ctcaattttc gatgtttctt tctttttttt
     1141 ataaaattca cttgtagctg catcagcttt ttcatcagat tttttagctc taaattttgc
     1201 tcttcttctt tgtctattta gctttttaaa agtagctgta gcaatttctt taacttcttc
     1261 agcagagtat tttttttcta aaccaattaa attttttctt ggtggaatgt ctaatatatc
     1321 tttcgataac tcattataaa attctttttc ttcttctaaa tcttgtttta ttaattctct
     1381 gtaatctttt agtgttgtat gatcaacaag agttttactt ttttcttcac cacgttttaa
     1441 accaaattta ctatttgatg cattatatat atcttgtaat cttccatatg aatttttacc
     1501 acgccaaaag cttttgttgt ttaatttgtt gtcaactata ggtaaaatta atgcatgaat
     1561 atgtggggta ctttcatcta aatgcaaaac tgcattaaca cattttccgt aattttcttt
     1621 ttccaaaaac tcaatttggg ttttaaccca atctttcaaa ttgtcattat attcagatct
     1681 agttttcttt gaaccatctt tattgttaaa gaaaaattct ggtgatgcag ttaaaacgaa
     1741 ttcgttaaaa attactgtgt ctttatttct aatgacaata tcattatttt taatataact
     1801 cattatgtcg gatctagtat tttcagtacc gattaatatt tcattcaaat ttgatttttt
     1861 agaatttaca ttttcttcta taactattct catattatga ttatgtacag cttgtatagc
     1921 tgtttttttg tgtgattgtt ttctgattat tgaatacatt tttatttctc catattattt
     1981 atacaacccc catttttttt ggataaatcc aatatatgag aatgtcggaa acattctcat
     2041 gtggcgttaa atgccacctg tgcagctgtg taaggttttg acgttgaacc ggtgcaaacc
     2101 ggttcggggc ttgggtgggc tatgcccccc aacgaacgac tgagcgtagc tcggtctagt
     2161 gagtgataga ccgagaaagt aaactcaacg tatatattta aagttatcac tttaatatat
     2221 tctgtcaaat agctaccgcc tcgcctaacg gctcgttgct gaatcctctt taaagcaaaa
     2281 gtaaactttt actttaaaaa ggattgatca caataataat aaaatacatg aacaaaaaag
     2341 acgggttgcg atgtgggcaa gttcttgacc actcgcaggg gtggggggac ccagaacaag
     2401 tactcttgtt ctggtggggg gaacgggttt aggggttccc cccagtagct gtagaacgct
     2461 ctgtgacggg tttttgtagt tactttaacc gtgagaacct gcatacagct tgctgtatgt
     2521 gggttcgagg ggttggcctg cccggcgagg gcggttttag attttgattt tactcttaga
     2581 tcttaatatt tattaattct tgcagaaaaa ctcaaatcct aaaactcctc tctaagcctt
     2641 gtcacgtctg gctttgcccc attttttcaa aaaaacaaaa agtacaaagt tttatactct
     2701 atccttaaaa aagtaacgat taagtaataa gttttatact ctagagtaat aaactttatc
     2761 gttttacacc cataaaaacg ataaacacac taaaatttct agaattttat ttatcgtttt
     2821 t
//
