LOCUS       NZ_AP022512             2511 bp    DNA     circular CON 21-APR-2024
DEFINITION  Enterobacter hormaechei strain STN0717-64 plasmid pSTN0717-64-2,
            complete sequence.
ACCESSION   NZ_AP022512
VERSION     NZ_AP022512.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195982
            Sequence Read Archive: DRR199787
            Assembly: GCF_015137995.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter hormaechei str. STN0717-64
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 320x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015137995.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 04:25:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,094
            CDSs (total)                      :: 4,980
            Genes (coding)                    :: 4,904
            CDSs (with protein)               :: 4,904
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 24 of 76
            Pseudo Genes (incomplete)         :: 54 of 76
            Pseudo Genes (internal stop)      :: 10 of 76
            Pseudo Genes (multiple problems)  :: 11 of 76
            Pseudo Genes (short protein)      :: 1 of 76
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022512.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="STN0717-64"
                     /isolation_source="sewage"
                     /db_xref="taxon:158836"
                     /plasmid="pSTN0717-64-2"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-07-17"
     gene            102..329
                     /locus_tag="JLF41_RS25350"
                     /old_locus_tag="STN0717ENT64_P20010"
     CDS             102..329
                     /locus_tag="JLF41_RS25350"
                     /old_locus_tag="STN0717ENT64_P20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            696..881
                     /locus_tag="JLF41_RS25355"
                     /old_locus_tag="STN0717ENT64_P20020"
     CDS             696..881
                     /locus_tag="JLF41_RS25355"
                     /old_locus_tag="STN0717ENT64_P20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2042..2185
                     /locus_tag="JLF41_RS25550"
     CDS             2042..2185
                     /locus_tag="JLF41_RS25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     Typing          complement(929..1008)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          1647..2207
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.339"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 aactcgatat gcagcccacc atcaagcctc ttttcacata aaaaatgacc tatagttaga
       61 tggttagtga ccaaaactga tatggataaa aggagctggc tttgaaaaag atttttgttt
      121 ccgtgttcgc agcggcagtt gcgctttcag ccctcaccgg ctgcacgcgc acaagctatg
      181 ccatacacac caatgacggg cgcactattg tcagcgacgg caaacctaaa gaatcagact
      241 caggcctgct tggctataca gacgcgaatg gtgtcaaaca gcagatcaat aaaaccgacg
      301 ttaaagaagt ctccgaaatt ccgcactgag catcttctcc gaaaaaggcc gctaatgtgg
      361 cctttttctt tacctggggt tctccgccaa aatcccagca aattgcacca ccaaagctaa
      421 aagcttccag ggttgtctaa aaagcacaag acattagagg aagtgcggtt tttataatca
      481 agccccccaa gaatttacca taacatccgt tgcccgcacc gcctcagacg cactcagcgc
      541 ttttctgacg gaaccatgac caaaaaccac aacaaaccac cccggaaccc gccagaaacg
      601 ctcctgttaa attttaacgg catgcatgac tatgcaccag aatgacggca tgctcttttc
      661 acgcaaaaat catcaccaga cggggaaaat caccagtgac cagacaggaa tccgccgccc
      721 tcaacatggc caaatttatc cgctcacaga cactcctcct cctcgagcgg ctcgaacaga
      781 tggatctgga tgaggctgcc ggctgctgtg agcacctgca cgatcaggcc gaagcacttt
      841 acaccatgct gaacgcacag acaggcgagg aagatgcgtg aaaatcggtg aacgggtgcg
      901 caattcagtg cgcggccgtg aggcgatggc ggggtgtcgg ggcgcagccc tgacctgggt
      961 atttgtgatg ccggcgcgtg cgcggtatta caaatacaca tcctgtcctg agtggttccc
     1021 cgggaacagg caaaaaaaaa ccggggagct gcccggaagg taagtcaagt tagattagat
     1081 tactttcact catccataac agcatcatgc aacgacgacc atcgtccgtg acggccgcct
     1141 cgtttaagta tggacagaaa tacagaaaat gctcaggacg aaatgtaatg aatgcgaacg
     1201 gattcaagaa attcgagcat gaccgtcctt acggccggtt cagtttcaga caaaatctgc
     1261 cggtatgcat ccagcatcat ggctccggca tccccccggc acgccggagc cagcccgaaa
     1321 caacagacac aaacaggtgt cgctcatcat caagagtcat caggtctccg gaagaaaaac
     1381 caaacatggc ttacctcctg ttaacggcaa cgcagaagaa aggtttacag ggtagcgggc
     1441 aggaaaataa aaaaattgca gtcagcccgg aacggctgct gaaccggtac aggcgggttc
     1501 cgggcgcagc gccggaccag tcactcagcg ctagcggagt gtatactggc taaaatgtaa
     1561 caccgcagta acgcatagca taactgctac ataagacagg cgcagaaaat gaaagaaaac
     1621 gcgtcaggag aaaatgtaat acaggatata ttccgcttcc tcgctcactc actcgctgcg
     1681 ctcggtcgtt cggctgcggc gagcggtacg ggcttacggg tggagcagaa atttcctagg
     1741 ggatgccagc taggggatgc caggaagata cttagcgaag aaggcataag gccgcgccga
     1801 agccgttttt ccataggctc cgcccccctg acgagcatca cgaaatctga cgctcaaatc
     1861 agtggtggcg aaacccgatg cggcttaaag ataccaggcg tttccccctg atggctccct
     1921 cgtgctcccc cgcattcctg cctttcggtt taccggtgtc actccgctgt tatggccgcg
     1981 gtttctcatt ccacgcctga cactcggttc cgggaaggca gttcgctcca ggatggactg
     2041 tatgcacgaa ccccccgttc agtccgactg ctgcgcctta tccggtaact atcgtcttga
     2101 gtccaacccg gaaagacacg taaaaacgcc actggcagcg gccactggta aggggtatac
     2161 aagattactg gtgcagagtt cttgaagttg acgcctgact gcggctacac tagaaggaca
     2221 aatttggtgg cagcggtctt gtacaccctg ttaccacggt tcagaaactt gccggtttgc
     2281 tgaaccttcg aaaaaccacc ttgccgggtg gttttttcgt ttacagggca agagattacg
     2341 cgcgaaaaaa aaggatctca agaagatcct tttataaatt agacatccaa cctcacaaag
     2401 aggaatactt aaatcatctt ggtatatgta tcggtcggat tacatcagat ataagcccgc
     2461 actgccccct ccccgccaga caccacactt taaccataac acctcgcacc t
//
