LOCUS       NZ_AP022506             3360 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STN0717-53 plasmid pSTN0717-53-8, complete
            sequence.
ACCESSION   NZ_AP022506
VERSION     NZ_AP022506.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195972
            Sequence Read Archive: DRR199777
            Assembly: GCF_015137465.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STN0717-53
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 315x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:58:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,316
            CDSs (total)                      :: 5,203
            Genes (coding)                    :: 5,103
            CDSs (with protein)               :: 5,103
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 71 of 100
            Pseudo Genes (internal stop)      :: 15 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022506.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STN0717-53"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTN0717-53-8"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-07-17"
     gene            complement(102..488)
                     /locus_tag="JLH17_RS26455"
                     /old_locus_tag="STN0717ENT53_P80010"
     CDS             complement(102..488)
                     /locus_tag="JLH17_RS26455"
                     /old_locus_tag="STN0717ENT53_P80010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045282373.1"
                     /translation="MRISNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDAAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     gene            655..1590
                     /locus_tag="JLH17_RS26460"
                     /old_locus_tag="STN0717ENT53_P80020"
     CDS             655..1590
                     /locus_tag="JLH17_RS26460"
                     /old_locus_tag="STN0717ENT53_P80020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_045626452.1"
                     /translation="MATYHLSVKFGGKGKALAHASYITREDKFSQRQDLEHTEHGNMPE
                     WARDEPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLALVRDFVKQETGDKHAWT
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKTQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            1593..2123
                     /locus_tag="JLH17_RS26465"
                     /old_locus_tag="STN0717ENT53_P80030"
     CDS             1593..2123
                     /locus_tag="JLH17_RS26465"
                     /old_locus_tag="STN0717ENT53_P80030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     Typing          656..1587
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.177"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcctatccga aaatccctac cgctcgccgc agccgaacga ccgagcgacg cgagtcagtg
       61 agcgaggaag cggcatttct ccggccttgc agaatgcacg ttcacgccga tgtttccaga
      121 tcgtagcgta cccgtttcag cgcagtcagt aatttttcgt ggtcaaaatt caactcaccc
      181 gtttttgtat caaccagtcc cgcgtcaatc atcagaccag ccgccagaaa cagtctgtga
      241 ttgcgttccc tgcggttttt ctgccctgaa atccgcgcct gaaccgcctt tttctgtgcg
      301 gcatcccgtt cctgcaacgc gtttttttca gcggttgcca gaacgtggag cagtcgtctt
      361 tcagtctcac tgagcgaagc ttcatcatcc agcagatcga taatctgccg ttgccgtgcc
      421 gttttttctc ccagctttct gatgaaggct atgcgctttt taagccattc gacgttactg
      481 atccgcatcg tttaacttcc ccatcccgat atttccgacg gggaaagtat ataacttgcg
      541 gtcagaagtc gcatgactta tctttactca taattcttta aattagagta aagcgcactt
      601 atatgtttct ccctgcgggc agaaacagtg cgccctgccg ggggctgaca caacatggca
      661 acgtatcatc tcagcgtgaa atttggcggc aagggtaaag cccttgctca tgccagttac
      721 attacgcggg aagataagtt cagccagcgc caggatctcg agcacaccga acacggcaat
      781 atgccggaat gggcgagaga tgaacccgcc catttctggc aggctgcgga tgcgttcgaa
      841 cgtgccaacg gttcaacgta cagggaaatt gagatcgctc taccccgcga gctgaatgaa
      901 gcgcagcggc tggcgcttgt ccgggatttt gtgaagcagg aaacgggcga caaacatgca
      961 tggacgtttg cgatccacaa tccgaaagcc agcattgacg gcggcgaaca gccgcacgcc
     1021 catatcatga tgagccagcg cgtcaatgac ggtattgagc gcagtcccga acagtatttc
     1081 aggcgctata acgccaaatt tccggcgcgt ggcggcgcgc gcaaggactc agggcataat
     1141 ttaaccccta cagaacaaaa acaggcactg aaagccctgc gggagcgctg ggagcgtacg
     1201 cacaacgcgc atatgaaacg atatggcttt gagcagggtt ttattgatag ccggtcactg
     1261 aaaacacagg gtatcgaccg gaaaccggaa cagcatctcg gttttgaagc agccggacga
     1321 ctggacgaca gccagaggca ggatattaaa gacagccgga cgttttcagt cgtggatgtg
     1381 cctgaacagg cgaaacatat cgaccacagc gaccgtagct ggatgaatgc cagcggtatc
     1441 gaggtaaacc ggaaggaact ggtcagatct ctgacgcatg atatctcgca gcagtacggg
     1501 cgggagatgg ctgcacgggt cacaaaacag ggaaatgttg aaatagagcg cgtgaaatcc
     1561 ctgtcacgcg gattttccat aagcaggtaa ctatggcgag caccgaagaa aaacttctca
     1621 cactcctgct gtcggcggag catctgaaag aggccgcaaa agagcagcag aaagccgtgg
     1681 cggattcgct ggcggtggca agggaaaccc tgaaaggcta ccgggaaata tcggacagtg
     1741 tggcacagca ggtaagggat gaagtccggc aggaagtgca gaaaatggat gttgcggggc
     1801 tgattggcga gcgtatcagc gctgattttc agcgtctgga aaaatccgtc gggaacatga
     1861 aaatcaatgc ggacgcgctg gacaaggaaa tttccattat ccgcagtaac atcatcgcgg
     1921 agtacaacac gctgcggggt tacagctgga agtggtttgc tggggttgcg gttgcctttg
     1981 tcgtactgat atttgccaca agctggtttg tgaaaacgga actacaggac aattacaacg
     2041 cgtctgctgc cgtctatcag aaagtggcag accttgaaaa gctgctgaca acggaacaga
     2101 aaccggaaaa gaaaaagaac tgacgcgctg cgcttgtcca acgcctcacg ttcgcacaat
     2161 aaattgcgca cccgttcacc gttttcagcc gagtttgggg cgtcttttga gacttccggc
     2221 ctgaattcag gccggaagtt ctcgccagtc agtcaggtgc atggttatgc atggggctga
     2281 aaatcacggt atgcgattct gagcggtttt cggtgagaag ccgggggttt ttatggctca
     2341 ggacttaaaa ccgccctgag agcctctgag gcggtgcacg caacggatgt tatggtaaat
     2401 ccggtggctt tgacgtgcct gccgttgtgt accgtcgtga gtgaaaataa ttatattgtt
     2461 cgaagaacag cgctttttgg ccggagaaaa gcagttctgc aaacgatggt atatcgatat
     2521 gagttctttt ggcattaatc caggcaagac gccgggcaat aagcgtaata ttccggatgt
     2581 tacttatttc catttctgtc aacagcatcc ccccccacgc cggagaataa aaaagccgcc
     2641 tgttccatcg gcaagcccta catcaaggga aactacgtct tacgattcgt tttgaaccgg
     2701 gacgctttgt tgctgcgaat cactaaccag ataatccacg ttttgtgagt gggtttaggt
     2761 taaggcttca atagaacaat aatagcgtta agtgattagg gcaacctgtg attgctaaaa
     2821 tgatgaagtc ttcacatcat ttcggtgaag gcgatatgtg ttacaccaca acccggattt
     2881 gagagtcgca aacccatatt agggcaaaga aagcctaatg cccccgtggt gggtacacca
     2941 gaaaacgggg agcgaagcga ccagcatcag gccgttcgta tgctgcttcg attttgtggg
     3001 tgttgcgcca gaccttggca atcacgcttc accgcaaccg ctgccttcac tctcacttta
     3061 tcaggtacgt tattggttaa cgtgagaccg ggtagagcca gcttctttcc cgagctggtc
     3121 aggttcccac cgcttgcctg tttatcctgc cggagtccgg ggcgtagacc tgagagtcaa
     3181 ggtgcagata tgggtttaat gtcgttgaat ggtattaagc tggtagtgac attcctagcc
     3241 agccctgcta ggatttgatt gtgacgtctg atcctggcag gtatctgcaa gaattagagt
     3301 actcaagccc cgtgattctg tcacggggct tttgtacatc tggagcaaga aaattcatgc
//
