LOCUS       NZ_AP022505             4161 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STN0717-53 plasmid pSTN0717-53-7, complete
            sequence.
ACCESSION   NZ_AP022505
VERSION     NZ_AP022505.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195972
            Sequence Read Archive: DRR199777
            Assembly: GCF_015137465.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STN0717-53
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 315x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:58:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,316
            CDSs (total)                      :: 5,203
            Genes (coding)                    :: 5,103
            CDSs (with protein)               :: 5,103
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 71 of 100
            Pseudo Genes (internal stop)      :: 15 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022505.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STN0717-53"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTN0717-53-7"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-07-17"
     gene            complement(102..371)
                     /locus_tag="JLH17_RS26430"
                     /old_locus_tag="STN0717ENT53_P70010"
     CDS             complement(102..371)
                     /locus_tag="JLH17_RS26430"
                     /old_locus_tag="STN0717ENT53_P70010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="WP_001324694.1"
                     /translation="MRRSTIRKGLTQKEMAERFNISVKTVKKYTAEPREQYEQTAADRR
                     RQAYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPKNSL"
                     /name="response regulator transcription factor"
     gene            complement(368..1225)
                     /locus_tag="JLH17_RS26435"
                     /old_locus_tag="STN0717ENT53_P70020"
     CDS             complement(368..1225)
                     /locus_tag="JLH17_RS26435"
                     /old_locus_tag="STN0717ENT53_P70020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_063153153.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEQVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQKKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPQNPLQYKELKATARSIARWTWKNFDSHTFSLIQSSRGAKNKNKPKKSNVKEMLGFI
                     GGEK"
                     /name="replication initiation protein"
     gene            complement(1764..2957)
                     /gene="mobQ"
                     /locus_tag="JLH17_RS26440"
                     /old_locus_tag="STN0717ENT53_P70030"
     CDS             complement(1764..2957)
                     /gene="mobQ"
                     /locus_tag="JLH17_RS26440"
                     /old_locus_tag="STN0717ENT53_P70030"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_063153152.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYLDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQREWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANVTYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNLNKKKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     gene            3368..3748
                     /locus_tag="JLH17_RS26445"
                     /old_locus_tag="STN0717ENT53_P70050"
     CDS             3368..3748
                     /locus_tag="JLH17_RS26445"
                     /old_locus_tag="STN0717ENT53_P70050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_213070177.1"
                     /translation="MEYRGLDFEEQEFHDCLNILHKEIVSFLNENNMAMDFFQYPVEDI
                     CRIIFRDKIDLRTAVILDFGITTYDLSPLADHDPFVWRLLEWPLYDYENFQFVIVQKLA
                     SYELDKLNEIIQVEDNKDKRRL"
                     /name="hypothetical protein"
     gene            3750..4019
                     /locus_tag="JLH17_RS26450"
                     /old_locus_tag="STN0717ENT53_P70060"
     CDS             3750..4019
                     /locus_tag="JLH17_RS26450"
                     /old_locus_tag="STN0717ENT53_P70060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_063153150.1"
                     /translation="MSKSTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LNIKPEEINKALVLGLLSKINELDEEEKRDFTRIGMDILQQWKS"
                     /name="conjugal transfer protein TraD"
     Typing          complement(1948..2957)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.11"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 tcacctatcc agacacaagc ccggatacgg ggaaagggga aaaaatcccc ccaggggtgg
       61 agggtttggt taggttaggg ggaaaaactg cttagaagtc gttacaggct gtttttaggc
      121 atatctaagg caacataacg gcgatacagg gcaacggctc cgtgatacga acagcccatt
      181 ttgtcggcta cctgttgcca tttcagcccc tggcttcgta gttcgtaagc ctgacgacga
      241 cgatcagccg ctgtttgttc gtattgttca cgaggttcgg ctgtatattt ttttaccgtt
      301 ttaacagata tattaaatct ttcagccatt tctttttgag ttaaaccttt tcttattgtg
      361 cttcttctca tttttcacct cctataaaac caagcatttc tttaacatta ctcttcttgg
      421 gtttgttttt atttttcgca cctcttgaag attgtattag tgaaaatgta tggctatcaa
      481 aatttttcca agtccaacgg gctatgcttc ttgctgttgc ttttaactct ttgtattgta
      541 acgggttttg tgggttgcaa aaagtattta aaccctgtgc ttttaatagt agttcgtttt
      601 cgaatttctc gaaagtagat atttttttga aatttaaaac cgttctgtaa gcatattggc
      661 gtagatcttc aaacaaatta acattgcgac ctaaaccata agcctcgctt tttttctttt
      721 gtatttgagg cttttctaag tccacaaaat cagctaaata atccaggtca taaaggtcag
      781 cacctgacca gaacggcgac cagtgatcat tgagtgggtt ttttgtaatc aatccagcaa
      841 accccagatc tgctttgagt tttttcgcca tagccatttc aacggctgcg gcatacttca
      901 acggcttcaa ccttgcttgt tgtgtcttac aaaccccggc tttcagcata tagccgtagt
      961 gacaacgacc tgtctttggt gtttttgtaa taaagtgagg tatgggtaag cccacctgct
     1021 caaatgtata gaatgaattt ttcttatcta aatcaaagag taagcaagtt tgaatggctg
     1081 gctgatttgc ctgtaaatac agcatttcaa gggcttttgc cttgttcctt atgcttaatc
     1141 ccatatcgag gttatttgtg caataaggtt tgttaggcat ttgattttta aaaaaatcga
     1201 tgttatgttc ttcttgtatt atcattttta ttcagtcctt ttttatctgg ccctgctcct
     1261 ctgttctgtc gccaaaacaa tcacagaggg gtggggtttt tatttttata gatttttcaa
     1321 tcctgtacca gttctaaccg atcttaaaaa tttgtcaagt ggggagctta accatatcag
     1381 ataacagccc aacgaagttg gggcagttct ttacatacgg cagcaaagca ttactatcag
     1441 atctatggtg cgaagcatta ccgaagatag aaaaacaata aaaaaagaaa aaagagtatt
     1501 gttcgaagaa aaattggcga ttcgattttt ttaggtgaat atatgaaaaa aaagaaagtg
     1561 cagaaaaaac ggaaaagaaa taagcatagt aaatttttta aaatgcttat gaagtcagtt
     1621 aatgaagctg tggcaataca taaaggggaa atagaaccgg cgaggatcac aactctaaga
     1681 atgtagtata ttatagtata gtattaaata atattaagtt gtattaagtg atactactat
     1741 atgggcatat ataccactat atactacatt ccaaatttct tcttcctctt aggttgctga
     1801 tcttcaaatt ccggcattgg ttccggctca ggctcttcaa tttttggttg tggttggttc
     1861 ataggagaca ttccagagcc tgaacccatt tttccgcctc cattatttcc accggttccg
     1921 gttcctgtgc ctgttttagg ttttgtttct aactctgttt ttaatatatg ttcgccaaat
     1981 tcatttatta cataactact aacgaaattt tgaggatctt ctttttcttc ttttttcttt
     2041 ttatttaaat taggttttga tttaaatata tgctgggtct ttttgtataa tgcctttaaa
     2101 ttatcaatga ttgaccttgc aagaggcttt ttctctattt tcatagtttc ctctggctga
     2161 ggtggtttgg tttgctgcaa gtaggttaca ttagctattt ttaataataa tgctttttct
     2221 ttttgctcca tagctcttaa tttttgacct tttcgccatt ggtgcctgtg tatcctggtc
     2281 attggcgttc tgtccagttc aatggttttt gaaatccact tggctttctc tttcggagtt
     2341 ttagcaaaaa cagcctttct ttctgcctct tctttttggt cttttaatga acgggagtca
     2401 atacgagatt tatgaccata tttttttaat cggtcatttg ctattatttc ccagttaact
     2461 cgccaggctt caatgttttc tctattattc cattctcttt gtttgttccc aaatccattt
     2521 gggctaacag ttcttaaagt tagcattaca tgagcatgag ggttatgact atcaagatca
     2581 tgaaatgcta tgtctgcaat cattccataa tccacgaaat tttcttggca gtaatcgagg
     2641 acgatctgct tcttctggcc gtcgtccagc tccttgggaa tggctatgtc cagataacgg
     2701 catagttcgg catcctttcg cttctcgcca gcctctacgg cattccagag ggtgcctgag
     2761 tccttcacaa gttcggcagg tgtgccggca ggggagagga tagcagagcc gaaaaatccc
     2821 tctttttttg aataatccca ggttgccccg gttcgattat cttttgtctt ttctcggttt
     2881 tgatatgcag ataaataaac cgaagattta ccttctgatc ttcgtaattt tttaaagctg
     2941 atatgaaatg tcgccatagt ctacccctta ctttttttgt cttttgaatt ttggttttta
     3001 gccccgcagg gcccatacgt cgcttgcgac gtgtaagtgg gcattactaa tattattttt
     3061 tcggttgcac ctcaaaataa taaagtaata aacccaggct tcgcccaata taaatatatt
     3121 aaaatatatt tgactaaagt aaataatgga ggtaatgaat aataaaataa ataattagga
     3181 ggttatatgg aaaaagaaac tctcggtcct gatatacaag tcaattggtg tgatcattgt
     3241 aaaaaaaaca ctcgccatat atttagattt actgaggagt attcaaaaga agagggacca
     3301 attacagagg ttgagtgtac taaatgtggt aatacattca ctatcttata gtgtggaggt
     3361 ttttaatatg gaatatagag gattagattt tgaagaacaa gaatttcacg actgtttaaa
     3421 tatattacac aaagagatag tgtctttttt aaatgaaaat aatatggcta tggatttttt
     3481 tcaatatcca gttgaggata tatgcagaat aatttttcga gataaaatag atctgagaac
     3541 tgctgttatt ttagattttg gaattacaac ctatgattta agccctttgg cagatcacga
     3601 tccttttgtt tggcgtttgc ttgaatggcc attatatgat tatgagaatt tccagtttgt
     3661 gattgttcaa aaactggctt cttatgaatt agataaatta aatgaaataa tccaagtaga
     3721 agataataaa gataaaagga ggttgtaata tgagcaaatc aacagcacag cagatcgcag
     3781 atctacaaaa taaactacaa gcattaagaa caaaaaaagc aaggcaggac agaagggaag
     3841 aaacaagaca aaaaataatc ctgggtgcag aagtggctaa ggttttaaat attaaaccag
     3901 aagaaataaa taaagctctt gtattaggct tgttaagtaa aatcaatgaa ttagacgaag
     3961 aagagaaaag agattttaca cggatcggaa tggatatatt gcagcaatgg aaatcgtaaa
     4021 ccgtgaacag tgaagaacca gccgcctttg gctggttttt cattgtgaag ggttgccagc
     4081 cgtaggctgg aggttttaag gttgccagag ggcctctggt ggctgggtgg gtttgttgat
     4141 ttgttggtac cagagtgtcc c
//
