LOCUS       NZ_AP022504             4426 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STN0717-53 plasmid pSTN0717-53-6, complete
            sequence.
ACCESSION   NZ_AP022504
VERSION     NZ_AP022504.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195972
            Sequence Read Archive: DRR199777
            Assembly: GCF_015137465.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STN0717-53
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 315x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:58:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,316
            CDSs (total)                      :: 5,203
            Genes (coding)                    :: 5,103
            CDSs (with protein)               :: 5,103
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 71 of 100
            Pseudo Genes (internal stop)      :: 15 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022504.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STN0717-53"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTN0717-53-6"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-07-17"
     gene            complement(91..552)
                     /locus_tag="JLH17_RS26395"
     CDS             complement(91..552)
                     /locus_tag="JLH17_RS26395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128348982.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FEMPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMVIRHYYVDCHPYYDRLPPEK
                     SDDFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            886..1101
                     /locus_tag="JLH17_RS26400"
                     /old_locus_tag="STN0717ENT53_P60020"
     CDS             886..1101
                     /locus_tag="JLH17_RS26400"
                     /old_locus_tag="STN0717ENT53_P60020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015387220.1"
                     /translation="MGFWETAGKIAKGAANALDEKAHELQATKMRLESKSSEELKRLIK
                     SDGFFGSASNTEKSMAMKILRERGDV"
                     /name="hypothetical protein"
     gene            complement(1789..2004)
                     /locus_tag="JLH17_RS26405"
                     /old_locus_tag="STN0717ENT53_P60040"
     CDS             complement(1789..2004)
                     /locus_tag="JLH17_RS26405"
                     /old_locus_tag="STN0717ENT53_P60040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2011..2502)
                     /locus_tag="JLH17_RS26410"
                     /old_locus_tag="STN0717ENT53_P60050"
     CDS             complement(2011..2502)
                     /locus_tag="JLH17_RS26410"
                     /old_locus_tag="STN0717ENT53_P60050"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2537..3241)
                     /locus_tag="JLH17_RS26810"
                     /pseudo=""
     CDS             complement(<2537..3241)
                     /locus_tag="JLH17_RS26810"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3180..3503)
                     /locus_tag="JLH17_RS26420"
                     /old_locus_tag="STN0717ENT53_P60060"
     CDS             complement(3180..3503)
                     /locus_tag="JLH17_RS26420"
                     /old_locus_tag="STN0717ENT53_P60060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_126289757.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4151..4261
                     /locus_tag="JLH17_RS26425"
     CDS             4151..4261
                     /locus_tag="JLH17_RS26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063858821.1"
                     /translation="MHEPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     Typing          complement(3617..3790)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.714"
                     /coverage="100.0"
                     /evalue="1.2e-79"
     Typing          3714..4318
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.196"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1698..3190)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.6"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3187..3503)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.34"
                     /coverage="99.0"
                     /evalue="8.540000000000001e-63"
ORIGIN
        1 tcgttttcag agcaagagat tacgcgcaga gcaaaacgat ctcaagaaga tcattttaca
       61 aattggataa tatccctccg ccccctcaca ctaatataaa attatcgggc ttgcatgccc
      121 ctcaccattt tccccatatc caccaccaaa caatagttcg tcgagttctt tgctggcttg
      181 atcaatacac gcagccacat gaatagcaga agggtattca tcatactgaa aatcatcgct
      241 tttttcagga ggaagtcgat cataatacgg atgacaatcc acataataat gacgtattac
      301 catcccagtt ttatcttcgt actctgatgg ctgcaacgta ctccctggga aagcttttgc
      361 aacatgtata gcagcttgaa aggtatcaaa atatcgccca aacattggca tttcaaagct
      421 aggcgactct gtcgaaaaat cccccataac ttgcgccaaa taaccattat ctatctgcca
      481 ttgaacatct gatcccatag cccactctcc cagagttact cctaataaat aaccataata
      541 tgtatttacc ataacgcttc acttcgcgaa ccagcacttc ccacagtaaa tcccttacct
      601 agctaacctt ggcacacgaa aaccatcata aaggcaacat atcagcttag ggttatctac
      661 ctctcaagct ccgagaaaaa ctgccgataa ttttagtgca ctatacaaat atttttaaaa
      721 tcaatggagt attcaatggc accgacaatc taccgggcta agtgtttgga ttgtaagcat
      781 gagtggacca gtcgtactgg ttatggcacg cctgattatt gccctaaatg tcggagtcgt
      841 cggatcacaa caagtccaat tggataagca ttaaggagtg gttaaatggg attttgggaa
      901 acggccggta aaattgcaaa aggcgcagct aacgctttgg atgaaaaagc tcatgaactc
      961 caggcaacca aaatgcgctt agaaagcaaa agcagtgaag aactaaagcg cctcattaaa
     1021 tctgatggtt ttttcggctc agccagtaac acagaaaaaa gcatggcaat gaaaattctc
     1081 agagaacgcg gagacgttta aggatagaaa aatggtaggc acggtgaatc ttcctcatac
     1141 atgccctaac tgcaagcgaa ccaccgctca tactgcaggt gagttgcaaa ctctattcgg
     1201 attcagaacc attccttctg gtgcaacgaa tcaaagttgg tgcagagaat gtcgtggtaa
     1261 gtcatagcgt ccagaagccc gtaacccggg cttttttacg cccacttatt taccataacg
     1321 tcccattgcc cgcaccgctg aaacggggtt ggctgcgttt ccggtgccag acgcgagaaa
     1381 gtggcgacag acatgacgaa aaaacaccag aaacgcagcc gctggatatt acactgatgc
     1441 atagtcatgc agccagatgc atggcgcgta aagtcacact aaaatcagcg cgaacgcctt
     1501 aaaaaccgac aaatgcttct ccagccagtc ccacagctcc gccgccagat acaggcagtt
     1561 aagcaatgat tcaataagat ttaacagctt catttcccgc tcctgaataa gtcgtgacct
     1621 ttttgatcac gaacctattt agcgggagaa gtaagagccc ctcttcgtgc gggttgtcag
     1681 ctgcctggat ggtttaaagt gtcggcccgc ggtaggtttt ctgcggctgg cgctccagct
     1741 ccagtgtttt ctgatactca ttctccctct gccggagctc gatggcattc agttcccgga
     1801 caagcggcgc agctgctcgc ttaaactcag cacctgcgtg ctcaagtccg acacgcgctc
     1861 gttcagcacc gcgttctccc gttgcataag cccacacatg ctccgccatt ccgcgaaggc
     1921 gctctcccat tcgtccagcc tttgcgagta gtcctgctgt agctgctcta atgcgctcag
     1981 caactgcttt tccagctctg tcattttccg ctactcctgt ttcaggcgga tccatgtcgt
     2041 cccgtcgtaa gggatggctt cggtttgcat ccccggcggc aggataagaa acctccggcc
     2101 gtcgctgctc tcctgatagc gcactcccca cgtccgggcg gtcattttcg ccagggtatc
     2161 ttcctgctga cgaagatgcg tgtaattgtc ggttatctgc tgcccctgcc accacagtat
     2221 gctcccgctc gtgccgatca gcagcgctga caccatcagg atggtgagcc atgtccgccc
     2281 ggcagtccgc agtacgctcc gccggttttt ctccatcgcc tcgctcatac tctgctcgtg
     2341 tgcgaggatg gcgtcgctga ttctcctcgc gcttgcgctc agttccgctc tgacagactg
     2401 ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta ctctgcttct gcgctttcga
     2461 ttgctgctct aagtccttcg ccagcgttaa aagactgctc atagatggct cccttcagtc
     2521 ggatgtttcg ccccccgtcc gggtcggcaa tgctgatact gcttttcgtg gtgcgcacca
     2581 cctcaaaacc tgaccgttcc agcgcctcag tgacgtcctg acgggttttc agctccccgg
     2641 acgaagcaag ggtcagcagt ccgcgcgtga tggcttctgc ggcctcctgc ttcgttttcg
     2701 gcagtccgga cggcgtgacc agcgcccggc ggttttccgg tgcgttcggg tcgtgcagcc
     2761 ccagtctgcc gttcacgatg gtctgccagg catcgatacg cggccggtcg gcgcggtcgt
     2821 agtacggctg gagccgcctg ccggtcagca gctcggtatt ggggatcaga aagttcagct
     2881 ccagacgccc cttgtcctcg tgctccaccc acagaatgct gtactgatct ttatcaagtc
     2941 cgggcatcag aacccgctcg aagctggcca tcagtttttc ccgctggccg ggaggcagat
     3001 cggcctccgc aaacgacagg acgccggagg tgtatttctt cacgtaaggc gacgcgtcaa
     3061 tcagctcccg gacttcttcc ggcttcccct gcaggacgct ggcaccttca cgctgccggt
     3121 ctttacccag aagatagtcc accggcccgg cgccaccgcc gcgaccgcgt ggatgaaatt
     3181 taacgatcat cgtccgttcc tttctcaaga acggcgtgcc gcagccgctc aagcccggca
     3241 tcgatggcca tcagcgccgc cacaatctgt accctgtcat ggccggtgcc gccgccggcg
     3301 ttaacacggc gggctatctg gttcaggtta ttgcccatgc cggcaagctg gcgcagcagc
     3361 gccggcgaga ttgaggggag ctttccggtg cgcgcaggct tttcgtccag gcaggtctgc
     3421 cgcatccagg ccgcgagctg tttaccgtcg cagcgttcca gcagacggcg gtgctcatct
     3481 tcggtcaccc acatcgtgag cattttgctg cgtttctctg acaaaccggt ctcctgataa
     3541 cgccgggacg ggcgggaacc tctttcccgg tcggcgcggg gttggacaag ccgcaggcgc
     3601 gtcaggcttt tggcgggttt cggggcgcag ccctgaacca gtcacgaagc gctagcggag
     3661 tgtatactgg cttactatgt tggcacggat gagggtgtca gtgaagtgct tcatgtggca
     3721 ggagaaaaaa ggcggcaccg gtgcgtcagc agaatatgtg gtacaggata tattccgctt
     3781 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggaa atggcttacg
     3841 agcggggcgg aaatttcctg gtagatgcga ggaagagact taacagggaa acgagaaggc
     3901 cgcggcgaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     3961 ctcaaatcag tggtggcgaa acccgacagg acttaaagat accaggcgtt tccccctggc
     4021 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcac tccgctgtta
     4081 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa
     4141 gctggactgt atgcacgaac cccccgttca gtccgactac cacgcccatt ccggtaacta
     4201 tcaacttgag tccaacccgg aaagacacga caaaatcgcc attggcggta gccactggta
     4261 agcaggatgt gcgagagatg tatcgagagt tcttgaagtg gtggccggac tacggctaca
     4321 ctggaaggac agatttggtg acacgtctcg cacaagactg ttaccacggt tcagaagttc
     4381 cccaacttac tgaaccttcg aaaaaccacc tccccaggtg gttttt
//
