LOCUS       NZ_AP022503             4918 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STN0717-53 plasmid pSTN0717-53-5, complete
            sequence.
ACCESSION   NZ_AP022503
VERSION     NZ_AP022503.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195972
            Sequence Read Archive: DRR199777
            Assembly: GCF_015137465.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STN0717-53
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 315x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:58:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,316
            CDSs (total)                      :: 5,203
            Genes (coding)                    :: 5,103
            CDSs (with protein)               :: 5,103
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 71 of 100
            Pseudo Genes (internal stop)      :: 15 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022503.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STN0717-53"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTN0717-53-5"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-07-17"
     gene            101..997
                     /locus_tag="JLH17_RS26360"
                     /old_locus_tag="STN0717ENT53_P50010"
     CDS             101..997
                     /locus_tag="JLH17_RS26360"
                     /old_locus_tag="STN0717ENT53_P50010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_064401065.1"
                     /translation="MRNAALELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRIGAAIDWSDRNAPAPTLTITNPENGHAHLLYALKTSIRTAPDGKMK
                     PLRYAAAVENALRKKLGADTGYSGLICKNPNHSHWKIAVWQPELYTLDWLADSLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEFDQWLQACYERASAYNLQFAAPLD
                     ENEVKWISKSVARWTHRKITKESFSDYVSRTHTSELQSFRGRKSHGGGRNKNPHSEREI
                     KPWDLLGISRATYYRRK"
                     /name="replication initiation protein"
     gene            complement(1006..1971)
                     /locus_tag="JLH17_RS26365"
                     /old_locus_tag="STN0717ENT53_P50020"
     CDS             complement(1006..1971)
                     /locus_tag="JLH17_RS26365"
                     /old_locus_tag="STN0717ENT53_P50020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200928270.1"
                     /translation="MWWSKIQIGIDLATSVTIFAAALTWWMNKVRERRVGVIDSARSTI
                     IENINNATNEMALSFNDFATFTTSIERKINTKLRFGEDKFIDEIASGRIDVVEIHTLFS
                     KSVDTMGVFYERGATLRYTIFPSLYSIGGEDEAVSILKKEINDVLVAYNKSQSGYIAYI
                     KEITSICEKIKKIKASDPSADLVSIIQDEYIGSIFSIVDDPDYGIFLQDFLDDGDDDLF
                     LISLHKKSEEMTDKEKELRLNVAKFLIYMIANKPESLISQFLQRLTFRMQENRVQCKEF
                     LVTLSAISSKMQQRSSDFSIKKAYDVLSSEKYFNTKEEIR"
                     /name="hypothetical protein"
     gene            2274..2600
                     /gene="mobC"
                     /locus_tag="JLH17_RS26370"
                     /old_locus_tag="STN0717ENT53_P50030"
     CDS             2274..2600
                     /gene="mobC"
                     /locus_tag="JLH17_RS26370"
                     /old_locus_tag="STN0717ENT53_P50030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_064401068.1"
                     /translation="MEKRTKEIKIRMTETEHQRLLERCDRLHLAEWLRQLGLGEQTSRK
                     RPVPEVAPELLRQISGMGNNLNQIARRLNQSDSLTPSERVSLLSLLNSLDRQLGELLEQ
                     HRDR"
                     /name="mobC"
     gene            2809..>3231
                     /locus_tag="JLH17_RS26695"
                     /pseudo=""
     CDS             2809..>3231
                     /locus_tag="JLH17_RS26695"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3278..3763
                     /locus_tag="JLH17_RS26700"
                     /old_locus_tag="STN0717ENT53_P50040"
     CDS             3278..3763
                     /locus_tag="JLH17_RS26700"
                     /old_locus_tag="STN0717ENT53_P50040"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_064401061.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTAQSVKREFEKLETSICEDLKSSS
                     QSISTAIREQNDQLTGIVRYSLLSALGWIFLFAALLIMILGGIIWYQGTVITSRLDEMD
                     SYSQQLESLKAQSGAGVAIYKDEKQKNTYMVVLPKKAHGTETYTSTSGNTVLKYTAK"
                     /name="MbeB family mobilization protein"
     gene            3833..4060
                     /locus_tag="JLH17_RS26380"
                     /old_locus_tag="STN0717ENT53_P50050"
     CDS             3833..4060
                     /locus_tag="JLH17_RS26380"
                     /old_locus_tag="STN0717ENT53_P50050"
                     /inference="COORDINATES: protein motif:HMM:NF016769.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_227540559.1"
                     /translation="MERQLLSALESLQTSYEQQQQAWQDSYSNLQRMFETTSQALERND
                     RVCLNLSNQLNGLYEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4205..4537
                     /locus_tag="JLH17_RS26385"
                     /old_locus_tag="STN0717ENT53_P50060"
     CDS             4205..4537
                     /locus_tag="JLH17_RS26385"
                     /old_locus_tag="STN0717ENT53_P50060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_064401063.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDELKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4539..4823
                     /gene="nadS"
                     /locus_tag="JLH17_RS26390"
                     /old_locus_tag="STN0717ENT53_P50070"
     CDS             4539..4823
                     /gene="nadS"
                     /locus_tag="JLH17_RS26390"
                     /old_locus_tag="STN0717ENT53_P50070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_064401064.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFHELAAH"
                     /name="nadS"
     Typing          2591..2871
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.17"
                     /coverage="90.0"
                     /evalue="6.29e-49"
ORIGIN
        1 tggcggatga gcatgattac cagggccagg tacgaaacct tgaaaggata ttagcacatg
       61 aaccgtgcca gacaacagcc cgccataagc gcagggcaca ttgcgtaacg cagcactcga
      121 acttttcaac gaccgcttac cgcacaaacc gtacttctcg gatgatttgc actttggtgt
      181 tcgcatcgca gggaaagaac gcgccattct ggcgaaatac atccagttca accagcctca
      241 cgccatgttc tggctgggat ttgacgttga ccgcatcggc gcagctatcg actggagcga
      301 ccgcaacgct cccgcgccta cactgaccat caccaatcca gaaaacggac atgcgcacct
      361 gctttacgcg ctgaaaacct cgatacgcac cgcgccagac ggaaaaatga aaccgttacg
      421 ctatgcagct gctgtagaga acgcgctacg taaaaaattg ggcgccgaca cgggctattc
      481 ggggctaatt tgcaagaatc cgaaccacag tcactggaaa attgccgtct ggcagcctga
      541 gctctacacg ttggactggt tagctgactc actcgacctg aacgccgcga acgacaaaga
      601 aattgtcgct gattatggct taggccgtaa ctgcaccctg ttcgacaaaa cccgcaagtg
      661 ggcatatcgt gccatccgtc agggctggcc tgagttcgac cagtggctgc aagcctgtta
      721 cgaacgtgcc agcgcctata atctgcaatt tgcagctcct ttagatgaaa atgaggttaa
      781 atggatttcg aaaagtgttg ccagatggac tcatagaaaa atcacaaagg aatcattttc
      841 tgattatgtg tccagaacac atacgtcaga actacaatct ttcagaggga gaaaaagcca
      901 tggcgggggg cggaataaaa atcctcactc agaaagagaa attaagccat gggatctgtt
      961 agggataagc agggctactt actacaggcg taagtagcct ttaaattatc ttatttcttc
     1021 ttttgtgttg aaatattttt cagaacttaa cacgtcatat gcttttttta tggagaagtc
     1081 actggaacgt tgctgcattt tacttgagat agcacttagt gttacaagaa actccttaca
     1141 ttgcactcgg ttttcttgca ttcgaaaagt cagcctttgc aggaattgag atatcaaaga
     1201 ttcaggtttg tttgcaatca tatatattaa gaatttcgcc acgttcaatc ttaattcttt
     1261 ttctttgtcg gtcatttctt ccgatttttt atgtaatgat attaaaaaca aatcatcatc
     1321 accatcatct aagaaatctt gtaaaaagat gccatagtca ggatcgtcaa caatactaaa
     1381 tatagatcca atgtattcgt cttgaatgat cgacaccaag tctgcgcttg gatcgctggc
     1441 ttttattttt tttatttttt cacatatgga tgttatctct tttatgtatg caatataccc
     1501 ggattgactt ttattatacg cgactaagac atcatttatt tcttttttta gaatagatac
     1561 cgcctcatct tcccccccta ttgagtataa tgatgggaag attgtatatc ttaatgttgc
     1621 ccccctttca taaaacactc ccattgtgtc tacgcttttt gagaaaagtg tgtgtatttc
     1681 aaccacatct attctccctg atgctatttc atcaatgaat ttatcttctc caaatcgtaa
     1741 tttggtgttt atttttcgct cgatcgatgt tgtgaatgtg gcaaaatcat taaaagataa
     1801 tgccatttca tttgtcgcat tgttgatatt ttctattatt gtggatcgag cactgtcaat
     1861 aacgcccact ctcctctccc tcaccttgtt catccaccag gttaaggctg ccgcaaagat
     1921 agttacactt gtcgcaaggt cgatgccaat ctgaatctta ctccaccaca taaacaattt
     1981 tcccttatag ttagctaccg cagattcaaa aaatacccaa agctgaaaac taatcaacac
     2041 gatttgagac caaaacaacc tatatcagat aacagcctct tttgcagggt ccgttttttt
     2101 gcagcttcca tagaatctgg catactgaaa atacccttta agccagtaca cacagcgtcc
     2161 cctctacgag gttccgtctg tgactggttc agggggctcg ccgccccccg aaacccccgg
     2221 catccactgc gaaaaattct cacagtggtg cgaatttttt ctcagcggag cctatggaaa
     2281 agcgaacgaa agagatcaaa atccgtatga ccgagactga gcatcagcgt ctgcttgaac
     2341 ggtgcgaccg tcttcacctt gcagagtggt tgcgtcagct tggtctggga gaacagacat
     2401 cacgtaaacg gcctgttcct gaggttgcgc ccgaactgct tcgacagatc agtggtatgg
     2461 gcaataacct gaaccagatc gctcgtcgac tgaatcagtc cgattccctg acgccttctg
     2521 agcgtgtctc actgctttcc ctcctgaaca gcctcgaccg tcagctaggg gagttactgg
     2581 agcagcaccg tgatcgttaa aatccattcc cgtggtgcag gttctggcag tggtccggtt
     2641 gattacctgc tgggaaaaga ccgtcagcga gagcaggcca ccgtattacg gggtgatcct
     2701 gaccgtgtat gcgaactgat agacagctgc gactttgccc gttcgtatac ctctggcgtg
     2761 ctgtcttttc aggagccgga tattgccgac tctgagaaat cccgcctgat ggacgagtgg
     2821 gaacagaccc tgatgacggg attagaccgt gaccagtaca actgtctctg ggtagagcac
     2881 cgcgacaagg gacgacttga gctgaatttt gtcatcccga acatcgaatt gcagagcgga
     2941 aaacggctac aaccctactt tgaccgcgcc gaccgcccca gagtgaatgc gtggcagacc
     3001 ctgaccaatg accgcctcgg attacgtgat cctaatgacc cccttaaccg ccgtccgatg
     3061 acgcaggcca gcgacttacc ccgcgacaaa cagcaggccg cagagaaaat caccgccggt
     3121 ctgatgaacc tgatgcagca gggtgagatc cgcagccggc aggatgtggt taagcagctg
     3181 gagagctacg gcctgactgt ggcgcgggaa accaaaaaca gtatcagcat tgccgatccg
     3241 gacggcggga aaaatatccg actgaaagga atgatttatg agcgagattt taaatttggc
     3301 gaagggcttc gaggagaaat cgaagcagca ggcgcaggat accgcgcaga gcgtgaagcg
     3361 cgagttcgag aagctggaaa cgtctatctg cgaggacttg aaatcaagct cgcagagcat
     3421 cagcaccgct atccgcgaac agaacgacca actgacgggc atagtcagat attccctcct
     3481 gagcgcactg ggctggatat tcctgttcgc cgcgctgctg atcatgatac tgggcgggat
     3541 aatctggtat cagggaacgg tcatcacttc ccgcctggac gaaatggaca gctacagcca
     3601 gcaactggag agcctgaaag cccagagcgg cgcgggggta gcgatttaca aggacgaaaa
     3661 gcagaagaac acctacatgg tggtactgcc gaagaaagcg catggcacag agacatacac
     3721 atcgacatcc ggaaacaccg tgttgaagta caccgcgaaa taaccccatc agacaccaca
     3781 gaaacgccac agcacgtttt tatcactgag gacaggcaaa accatgaccg aaatggaaag
     3841 gcagcttctg agcgccttag aaagcttaca gacaagctac gaacaacagc agcaggcgtg
     3901 gcaggacagt tacagcaact tgcaacgcat gttcgagact acctcacagg cgctggagcg
     3961 caacgacagg gtatgtctga acttgagcaa tcagctgaac ggtttgtacg agcaggtcga
     4021 acgcttgagc agcgatctga gccgattaac acgctcgtaa agcagcagga acggcagtta
     4081 cagcaggaga agcaagcaac cccctcacga ggttggtcta ggtaacgtct gatggtacag
     4141 aaagcggctt tcctgagcgt ggttgttggt gtacattgta ctctaaaata tacaggagac
     4201 agccgtgagt cacgcgattg agtttatcga gacaacgatg tttacccgtc agataaagca
     4261 aatcgccacg gatgatgaac tcaaagagct ccagaaagag cttatcgagt caccggacaa
     4321 aggtgacctc atccagaaaa cgggcggtct gcgaaagatc aggatggcga cggggaatca
     4381 gggaaaaagc ggcagtgcgc gggtgattta cttccttgcc accgctgaag tgatttatct
     4441 ggtgatggcc taccctaaaa gcaccaaaga cagcatcaca gacgcagaga aagccgaact
     4501 gaagaagctg acccaacgac tgaaagacga ggtgtaacat gagttttttt gacgagctga
     4561 aagcctctct ggaagaggcc gttgaaatca aaaacggaac caaagccccc gcccgtgtga
     4621 cccgctacga gctggcagac gtcagggcta tcagggaaaa cctgaatatt actcagagcg
     4681 aaatggcgaa agtgctggga accagcctcg acaccatcaa gagctgggaa accggacgcc
     4741 gtaacccgac cggactggcc gcaaaagtgc tggccaccat tcaggctaac ccgaaattct
     4801 ttcatgaact ggcggcgcat taagctgatg tgacagaaga aattcgacag gcgaggccgt
     4861 tcgcgtgtaa ctttaacggc agcagaaaaa acgaagcccc ggaaatcatg agctatct
//
