LOCUS       NZ_AP022502             4938 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STN0717-53 plasmid pSTN0717-53-4, complete
            sequence.
ACCESSION   NZ_AP022502
VERSION     NZ_AP022502.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195972
            Sequence Read Archive: DRR199777
            Assembly: GCF_015137465.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STN0717-53
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 315x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:58:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,316
            CDSs (total)                      :: 5,203
            Genes (coding)                    :: 5,103
            CDSs (with protein)               :: 5,103
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 32 of 100
            Pseudo Genes (incomplete)         :: 71 of 100
            Pseudo Genes (internal stop)      :: 15 of 100
            Pseudo Genes (multiple problems)  :: 15 of 100
            Pseudo Genes (short protein)      :: 1 of 100
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022502.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STN0717-53"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTN0717-53-4"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-07-17"
     gene            119..1405
                     /locus_tag="JLH17_RS26335"
                     /old_locus_tag="STN0717ENT53_P40010"
     CDS             119..1405
                     /locus_tag="JLH17_RS26335"
                     /old_locus_tag="STN0717ENT53_P40010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_306170312.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            2414..2737
                     /locus_tag="JLH17_RS26340"
                     /old_locus_tag="STN0717ENT53_P40030"
     CDS             2414..2737
                     /locus_tag="JLH17_RS26340"
                     /old_locus_tag="STN0717ENT53_P40030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007899679.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTAKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGVGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2946..>3380
                     /locus_tag="JLH17_RS26690"
                     /pseudo=""
     CDS             2946..>3380
                     /locus_tag="JLH17_RS26690"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3415..3906
                     /locus_tag="JLH17_RS26350"
                     /old_locus_tag="STN0717ENT53_P40040"
     CDS             3415..3906
                     /locus_tag="JLH17_RS26350"
                     /old_locus_tag="STN0717ENT53_P40040"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_016355729.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESNDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            3913..4128
                     /locus_tag="JLH17_RS26355"
                     /old_locus_tag="STN0717ENT53_P40050"
     CDS             3913..4128
                     /locus_tag="JLH17_RS26355"
                     /old_locus_tag="STN0717ENT53_P40050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2127..2299
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.14e-86"
     Typing          complement(1656..2202)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.364"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2415..2731
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="3.03e-59"
     Typing          2728..4229
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.692"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agcgggcttt ttcccgccct gtcgcagtaa tatataacgc tacataagga gtagccgata
       61 accatggcag tacttacatt atccgggcaa tctgattcat atgttttcac ttaaaaatat
      121 gttctccctg aacctccagc gatcagccat ttcgtatgac gcaatttgtc agaataagcc
      181 tgtcattgag ttcagtccgg agggggtgat aaataaagcc agcccgcttt ttctctccac
      241 tatggggtac agggctgatg aaataatcgg tcaccaccat cgtatattct gcccgccctc
      301 gctggtaagt tcaccggaat acagccagtt ctggcagcgc cttgccaggg gagaaagctt
      361 tagtggcaag tacctgcggc tggcaaaggg aaatcgttcc gtatggcttg aagccagtta
      421 tatccccgtt tctgacaggc gcggccgtgt tatcagagtc attaaaattg ctgccgatat
      481 ttctgagcgc gtgcattctg ctcttgagca ggaagcagtc gtaaatgcca taaaccgttc
      541 tatggccatc atcaccttca atcctgaagg gatcgtgctt gaagcaaatg aaaatttcgt
      601 caatgccact ggctataagc gggatgaaat cataggtaag catcatcgtc tgttctgtgc
      661 cgagacgctt tacaaaagtg atgaataccg acatttctgg gagagtctga atcaaggtga
      721 gtttttttct ggtctgtttc cacgccttaa ccgtcaggga gaccctctgt ggttccgtgc
      781 aacttataac cctgtcttta acagtgatgg gcagctttat aaaattgtga aattcgctac
      841 agatgttacg gctgatgtcc tgcgaaacca gagagagcaa gaggctgctg tgcatgcatg
      901 ggatatggcc gtgcagaccc gagaaagtgc gcagaacggt gccaatgtta ttgaaaatag
      961 catccttatg attgacagaa ttgcgcaggg gatgggggct gtctccaccg atatctcacg
     1021 gctcaacaat cagtctgaaa gtattgacga tatggtggaa accatccgga agtttgccat
     1081 gcagacaaga cttattgcac tgaatgccgc gattgaagca gcaagggctg gcgcatccgg
     1141 aagaagtttt gcggttgttg ccgcagaagt gcgtaatctt gcggcgagcg tcagtagcgc
     1201 gactgaagaa attgagcaag tcgtggccag caatagtcaa ctggccaagg atgttctctg
     1261 tggcattgaa aacagcctga tgaacactcg ggaaggggtt acactcatgc gcgaagcggg
     1321 tgaagttatg accagcattc agaggaatgc tgcaggggtt gagactgcgg ttaaggatgt
     1381 cgccatttca gttaaagccg gataggccat ggtgcatatc cccatatctg tcgaacatca
     1441 cagatgatct tcttgagatc ctttccgttg cgtcgtaatc tcttgctctg aaaacgaaaa
     1501 aaccacccgg caaggtggct ttttcgaagg ttcgcaagag ttgacgctct tttgaaccgc
     1561 ggtaactggc ttggaggagc acagtcacca aaactgtcct ttcagtgtag ccttatcgca
     1621 cccgaaactt caagactctc ctcctctaaa cctgttacca gtggctgctg ccagtggcgc
     1681 tttgtcgtgt ttttccggat tggactcaag ttgatagtta ccggataagg cgcagcggtc
     1741 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct tcccggaact
     1801 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
     1861 gaaaggcagg aacaggagag cgcacgaggg agccaccagg gggaaacgcc tggtatcttt
     1921 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag
     1981 gggggcggag cctatggaaa aacggcttcg ccgcggcctt atcgcttccc tgttaagctt
     2041 ctttctggca tctcccagga aatttccgcc ccgcctgtaa gccggtaccg ctcgccgcag
     2101 ccgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc tgtatcgcat
     2161 attctgctga cgctccgctg ctgcattttt ctcctgccac atgaagcact tcactgatat
     2221 tcgcatccgt gccaacatag taagccagta tacactccgc tagcgctacg tgactggttc
     2281 agggctgcgc cccgaaaccc gctaacagcc actgacgcgc ctgcggcttg tccaaccccg
     2341 cgccgaccgg gaaaggtttt cccgcccgtc ccggggttat caggagactg ttttgtcaga
     2401 gaaacggaac aagatgctca ccatgtgggt gacggaggat gagcacaggc ggctacttga
     2461 gcgctgcgac ggcaggcagc tggcggcgtg gatgcggcag acctgcctgg acgaaaagcc
     2521 ggctcgtacc gcaaaacttc cttcgatctc gccggcgctg ctgcgccagc ttgccggtat
     2581 gggcaacaac ctgaaccaga tagcccgccg tgttaacgcc ggcgtcggta ccggtcatga
     2641 ccgggtgcag attgtggcgg cgctgatggc catcgatgcc gggcttgagc ggctgcggca
     2701 cgccgttctt gagaaaggaa cggacgatga tcgttaaatt tcatccacgc ggtcgcggcg
     2761 gtggcgccgg gccggtggac tatctgctgg gtaaagaccg gcagcgtgaa ggcgccagcg
     2821 tcctgcaggg gaagccggaa gaagtccggg agctgatcga cgcgtcgcct tacgtgaaga
     2881 aatacacctc cggcgtcctg tcgtttgcgg aggccgatct gcctcccggc cagcgggaaa
     2941 aactgatggc cagcttcgag cgggttctga tgcccggact tgataaagat cagtacagca
     3001 ttctgtgggt ggagcacgag gacaaggggc gtctggagct gaactttctg atccccaata
     3061 ccgagctgct gaccggcagg cggctccagc cgtactacga ccgcgccgac cggccgcgta
     3121 tcgatgcctg gcagaccatc gtgaacggca gactggggct gcacgatccg aacgcgccgg
     3181 aaaaccgccg ggcgctggtc acgccggccg gactgccgaa aacgaagcag gaagccgcag
     3241 aagccatcac ccgtggactg ctgagcttag cctcgtccgg ggagctgaaa agccgtcagg
     3301 acgtcactga ggctcttgaa ggtgcaggtt ttgaggtggt gcgtacaacg aaaagcagta
     3361 tcagcattgc cgaccctgac ggggggcgaa acatccgact gaagggagcc atctatgagc
     3421 agtcttttaa cgctggcgaa ggacttagag cagcaatcga aagcgcagca gaagagtacc
     3481 ggcgagatgc tgaaagccgc attcagcgag cacgagcagt ctgtcagagc ggaactgaac
     3541 gcaagcgcga ggagaatcag cgacgccatc agcgcccacg agcagagtat gagcgaggcg
     3601 atggagaaaa accggcggag cgtgttgctg acggccggga ggacatggct caccatcctg
     3661 atggtgtcag cgctgctgat cgccacgagc gggagcattc tgtggtggca gggacggcag
     3721 ataaccgaca attacacaca tcttcgtcag caggaagata ccctggcgaa aatgaccgcc
     3781 cggacgtggg gagtacgcta tcaggagagc aacgacggcc ggaggttcct tatcctgccg
     3841 ccggggatgc aagccgaagc catcccctac gacgggacga cctggatccg cctgaaacag
     3901 gagtagcgga aaatgacaga gctggaaaag cagttgctga gcgcattaga gcagctacag
     3961 caggactact cgcaaaggct ggacgaatgg gagagcgcct tcgcggaatg gcggagcatg
     4021 tgtggactta tgcaacggga gaacgcggtg ctgaacgagc gcgtgtcgga cttgagcacg
     4081 caggtgctga gtttaagcga gcagctgcgc cgcttgtccg ggaactgaat gccatcgagg
     4141 agaaggctgc actggagtat cagcgtcaga acgagctgga gctgacgctc cgacagaagc
     4201 agaagagttt cgatggtccg aagatgaggt gacagcccgc acgaagaagg gctcatcgcg
     4261 ttcctgctaa ataggctgac taccggaatg gtagtgactt atcccggagg aagtgatgaa
     4321 aattctcaac gttattgaag ccctgctgaa ctgtttttac ctggtgatgg agctgtgtga
     4381 gttgccggag aggcatttgc cggtttttaa ggcgttcgcg ctgattttag tgtgacttta
     4441 cgctgcatgc atctggctgc atgactatgc atcagtgtaa tatccagcgg ctgcgttttt
     4501 ggtgtttttt cgtcatgtct gtcgccactt tctcgcgtct ggcaccggaa acgcagccaa
     4561 ccccgtttct gcggtgcggg caaggggtgt tatgttaaaa gccgaggtct gccggatacg
     4621 gaaaaaatta tgccggccgg gcttatgccc gcggcaatcc ggccgatact taaattatcc
     4681 ttcacaaagg atgtgctgcg atccaggcag ttgagtgttt tccggctccc ggatgtgagc
     4741 catttttttg ccttctgatg cctgttaatg catgcggagc tgcgctggtt cttccaagat
     4801 tgatagacgc cagcttctct gctggagccg gcggatttgc cgtaaatatg aacattttct
     4861 tattgttcag cccgctaaag atggcgggtt ttttacgcgt gaaatccgga gccggacatc
     4921 atgccggcat ggctgaag
//
