LOCUS       NZ_AP022472             4938 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter roggenkampii strain STW0522-66 plasmid pSTW0522-66-7,
            complete sequence.
ACCESSION   NZ_AP022472
VERSION     NZ_AP022472.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195906
            Sequence Read Archive: DRR199711
            Assembly: GCF_015136315.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter roggenkampii str.
            STW0522-66 isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 513x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:28:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,279
            CDSs (total)                      :: 5,169
            Genes (coding)                    :: 5,025
            CDSs (with protein)               :: 5,025
            Genes (RNA)                       :: 110
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 47 of 144
            Pseudo Genes (incomplete)         :: 99 of 144
            Pseudo Genes (internal stop)      :: 23 of 144
            Pseudo Genes (multiple problems)  :: 24 of 144
            Pseudo Genes (short protein)      :: 1 of 144
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022472.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="STW0522-66"
                     /isolation_source="sewage"
                     /db_xref="taxon:1812935"
                     /plasmid="pSTW0522-66-7"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            120..1406
                     /locus_tag="JLF42_RS26145"
                     /old_locus_tag="STW0522ENT66_P70010"
     CDS             120..1406
                     /locus_tag="JLF42_RS26145"
                     /old_locus_tag="STW0522ENT66_P70010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_306170312.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            2415..2738
                     /locus_tag="JLF42_RS26150"
                     /old_locus_tag="STW0522ENT66_P70030"
     CDS             2415..2738
                     /locus_tag="JLF42_RS26150"
                     /old_locus_tag="STW0522ENT66_P70030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007899679.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTAKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGVGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2947..>3381
                     /locus_tag="JLF42_RS26510"
                     /pseudo=""
     CDS             2947..>3381
                     /locus_tag="JLF42_RS26510"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3416..3907
                     /locus_tag="JLF42_RS26160"
                     /old_locus_tag="STW0522ENT66_P70040"
     CDS             3416..3907
                     /locus_tag="JLF42_RS26160"
                     /old_locus_tag="STW0522ENT66_P70040"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_016355729.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESNDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            3914..4129
                     /locus_tag="JLF42_RS26165"
                     /old_locus_tag="STW0522ENT66_P70050"
     CDS             3914..4129
                     /locus_tag="JLF42_RS26165"
                     /old_locus_tag="STW0522ENT66_P70050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2128..2300
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.14e-86"
     Typing          complement(1657..2203)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.545"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          2416..2732
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="3.03e-59"
     Typing          2729..4230
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.692"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagcgggctt tttcccgccc tgtcgcagta atatataacg ctacataagg agtagccgat
       61 aaccatggca gtacttacat tatccgggca atctgattca tatgttttca cttaaaaata
      121 tgttctccct gaacctccag cgatcagcca tttcgtatga cgcaatttgt cagaataagc
      181 ctgtcattga gttcagtccg gagggggtga taaataaagc cagcccgctt tttctctcca
      241 ctatggggta cagggctgat gaaataatcg gtcaccacca tcgtatattc tgcccgccct
      301 cgctggtaag ttcaccggaa tacagccagt tctggcagcg ccttgccagg ggagaaagct
      361 ttagtggcaa gtacctgcgg ctggcaaagg gaaatcgttc cgtatggctt gaagccagtt
      421 atatccccgt ttctgacagg cgcggccgtg ttatcagagt cattaaaatt gctgccgata
      481 tttctgagcg cgtgcattct gctcttgagc aggaagcagt cgtaaatgcc ataaaccgtt
      541 ctatggccat catcaccttc aatcctgaag ggatcgtgct tgaagcaaat gaaaatttcg
      601 tcaatgccac tggctataag cgggatgaaa tcataggtaa gcatcatcgt ctgttctgtg
      661 ccgagacgct ttacaaaagt gatgaatacc gacatttctg ggagagtctg aatcaaggtg
      721 agtttttttc tggtctgttt ccacgcctta accgtcaggg agaccctctg tggttccgtg
      781 caacttataa ccctgtcttt aacagtgatg ggcagcttta taaaattgtg aaattcgcta
      841 cagatgttac ggctgatgtc ctgcgaaacc agagagagca agaggctgct gtgcatgcat
      901 gggatatggc cgtgcagacc cgagaaagtg cgcagaacgg tgccaatgtt attgaaaata
      961 gcatccttat gattgacaga attgcgcagg ggatgggggc tgtctccacc gatatctcac
     1021 ggctcaacaa tcagtctgaa agtattgacg atatggtgga aaccatccgg aagtttgcca
     1081 tgcagacaag acttattgca ctgaatgccg cgattgaagc agcaagggct ggcgcatccg
     1141 gaagaagttt tgcggttgtt gccgcagaag tgcgtaatct tgcggcgagc gtcagtagcg
     1201 cgactgaaga aattgagcaa gtcgtggcca gcaatagtca actggccaag gatgttctct
     1261 gtggcattga aaacagcctg atgaacactc gggaaggggt tacactcatg cgcgaagcgg
     1321 gtgaagttat gaccagcatt cagaggaatg ctgcaggggt tgagactgcg gttaaggatg
     1381 tcgccatttc agttaaagcc ggataggcca tggtgcatat ccccatatct gtcgaacatc
     1441 acagatgatc ttcttgagat cctttccgtt gcgtcgtaat ctcttgctct gaaaacgaaa
     1501 aaaccacccg gcaaggtggc tttttcgaag gttcgcaaga gttgacgctc ttttgaaccg
     1561 cggtaactgg cttggaggag cacagtcacc aaaactgtcc tttcagtgta gccttatcgc
     1621 acccgaaact tcaagactct cctcctctaa acctgttacc agtggctgct gccagtggcg
     1681 ctttgtcgtg tttttccgga ttggactcaa gttgatagtt accggataag gcgcagcggt
     1741 cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc ttcccggaac
     1801 tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac accggtaaac
     1861 cgaaaggcag gaacaggaga gcgcacgagg gagccaccag ggggaaacgc ctggtatctt
     1921 tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg atgcttgtca
     1981 ggggggcgga gcctatggaa aaacggcttc gccgcggcct tatcgcttcc ctgttaagct
     2041 tctttctggc atctcccagg aaatttccgc cccgcctgta agccggtacc gctcgccgca
     2101 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaatatatc ctgtatcgca
     2161 tattctgctg acgctccgct gctgcatttt tctcctgcca catgaagcac ttcactgata
     2221 ttcgcatccg tgccaacata gtaagccagt atacactccg ctagcgctac gtgactggtt
     2281 cagggctgcg ccccgaaacc cgctaacagc cactgacgcg cctgcggctt gtccaacccc
     2341 gcgccgaccg ggaaaggttt tcccgcccgt cccggggtta tcaggagact gttttgtcag
     2401 agaaacggaa caagatgctc accatgtggg tgacggagga tgagcacagg cggctacttg
     2461 agcgctgcga cggcaggcag ctggcggcgt ggatgcggca gacctgcctg gacgaaaagc
     2521 cggctcgtac cgcaaaactt ccttcgatct cgccggcgct gctgcgccag cttgccggta
     2581 tgggcaacaa cctgaaccag atagcccgcc gtgttaacgc cggcgtcggt accggtcatg
     2641 accgggtgca gattgtggcg gcgctgatgg ccatcgatgc cgggcttgag cggctgcggc
     2701 acgccgttct tgagaaagga acggacgatg atcgttaaat ttcatccacg cggtcgcggc
     2761 ggtggcgccg ggccggtgga ctatctgctg ggtaaagacc ggcagcgtga aggcgccagc
     2821 gtcctgcagg ggaagccgga agaagtccgg gagctgatcg acgcgtcgcc ttacgtgaag
     2881 aaatacacct ccggcgtcct gtcgtttgcg gaggccgatc tgcctcccgg ccagcgggaa
     2941 aaactgatgg ccagcttcga gcgggttctg atgcccggac ttgataaaga tcagtacagc
     3001 attctgtggg tggagcacga ggacaagggg cgtctggagc tgaactttct gatccccaat
     3061 accgagctgc tgaccggcag gcggctccag ccgtactacg accgcgccga ccggccgcgt
     3121 atcgatgcct ggcagaccat cgtgaacggc agactggggc tgcacgatcc gaacgcgccg
     3181 gaaaaccgcc gggcgctggt cacgccggcc ggactgccga aaacgaagca ggaagccgca
     3241 gaagccatca cccgtggact gctgagctta gcctcgtccg gggagctgaa aagccgtcag
     3301 gacgtcactg aggctcttga aggtgcaggt tttgaggtgg tgcgtacaac gaaaagcagt
     3361 atcagcattg ccgaccctga cggggggcga aacatccgac tgaagggagc catctatgag
     3421 cagtctttta acgctggcga aggacttaga gcagcaatcg aaagcgcagc agaagagtac
     3481 cggcgagatg ctgaaagccg cattcagcga gcacgagcag tctgtcagag cggaactgaa
     3541 cgcaagcgcg aggagaatca gcgacgccat cagcgcccac gagcagagta tgagcgaggc
     3601 gatggagaaa aaccggcgga gcgtgttgct gacggccggg aggacatggc tcaccatcct
     3661 gatggtgtca gcgctgctga tcgccacgag cgggagcatt ctgtggtggc agggacggca
     3721 gataaccgac aattacacac atcttcgtca gcaggaagat accctggcga aaatgaccgc
     3781 ccggacgtgg ggagtacgct atcaggagag caacgacggc cggaggttcc ttatcctgcc
     3841 gccggggatg caagccgaag ccatccccta cgacgggacg acctggatcc gcctgaaaca
     3901 ggagtagcgg aaaatgacag agctggaaaa gcagttgctg agcgcattag agcagctaca
     3961 gcaggactac tcgcaaaggc tggacgaatg ggagagcgcc ttcgcggaat ggcggagcat
     4021 gtgtggactt atgcaacggg agaacgcggt gctgaacgag cgcgtgtcgg acttgagcac
     4081 gcaggtgctg agtttaagcg agcagctgcg ccgcttgtcc gggaactgaa tgccatcgag
     4141 gagaaggctg cactggagta tcagcgtcag aacgagctgg agctgacgct ccgacagaag
     4201 cagaagagtt tcgatggtcc gaagatgagg tgacagcccg cacgaagaag ggctcatcgc
     4261 gttcctgcta aataggctga ctaccggaat ggtagtgact tatcccggag gaagtgatga
     4321 aaattctcaa cgttattgaa gccctgctga actgttttta cctggtgatg gagctgtgtg
     4381 agttgccgga gaggcatttg ccggttttta aggcgttcgc gctgatttta gtgtgacttt
     4441 acgctgcatg catctggctg catgactatg catcagtgta atatccagcg gctgcgtttt
     4501 tggtgttttt tcgtcatgtc tgtcgccact ttctcgcgtc tggcaccgga aacgcagcca
     4561 accccgtttc tgcggtgcgg gcaaggggtg ttatgttaaa agccgaggtc tggcggatac
     4621 ggaaaaaatt atgccggccg ggcttatgcc cgcggcaatc cggccgatac ttaaattatc
     4681 cttcacaaag gatgtgctgc gatccaggca gttgagtgtt ttccggctcc cggatgtgag
     4741 ccattttttt gccttctgat gcctgttaat gcatgcggag ctgcgctggt tcttccaaga
     4801 ttgatagacg ccagcttctc tgctggagcc ggcggatttg ccgtaaatat gaacattttc
     4861 ttattgttca gcccgctaaa gatggcgggt tttttacgcg tgaaatccgg agccggacat
     4921 catgccggca tggctgaa
//
