LOCUS       NZ_AP022471             5370 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter roggenkampii strain STW0522-66 plasmid pSTW0522-66-6,
            complete sequence.
ACCESSION   NZ_AP022471
VERSION     NZ_AP022471.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195906
            Sequence Read Archive: DRR199711
            Assembly: GCF_015136315.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter roggenkampii str.
            STW0522-66 isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 513x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:28:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,279
            CDSs (total)                      :: 5,169
            Genes (coding)                    :: 5,025
            CDSs (with protein)               :: 5,025
            Genes (RNA)                       :: 110
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 47 of 144
            Pseudo Genes (incomplete)         :: 99 of 144
            Pseudo Genes (internal stop)      :: 23 of 144
            Pseudo Genes (multiple problems)  :: 24 of 144
            Pseudo Genes (short protein)      :: 1 of 144
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022471.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="STW0522-66"
                     /isolation_source="sewage"
                     /db_xref="taxon:1812935"
                     /plasmid="pSTW0522-66-6"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            complement(102..425)
                     /locus_tag="JLF42_RS26115"
                     /old_locus_tag="STW0522ENT66_P60010"
     CDS             complement(102..425)
                     /locus_tag="JLF42_RS26115"
                     /old_locus_tag="STW0522ENT66_P60010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_180257127.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1516..1992
                     /locus_tag="JLF42_RS26120"
                     /old_locus_tag="STW0522ENT66_P60020"
     CDS             1516..1992
                     /locus_tag="JLF42_RS26120"
                     /old_locus_tag="STW0522ENT66_P60020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032623641.1"
                     /translation="MNNESMEAKIASLNNIQSIINRLAETSSKLKATYITIISAVLTIV
                     IAYVIPQKPVASENFTTNEKLYSILIFLIITLAFMLIDSYYLRYERAMRKVYDLKAEDK
                     KDSDGKINYFEISNTFNYVLKNQNIKSRPSRSQFIFYVIPFLAIAFSIAVLATR"
                     /name="hypothetical protein"
     gene            1995..2828
                     /locus_tag="JLF42_RS26125"
                     /old_locus_tag="STW0522ENT66_P60030"
     CDS             1995..2828
                     /locus_tag="JLF42_RS26125"
                     /old_locus_tag="STW0522ENT66_P60030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032623640.1"
                     /translation="MHKKFHIELDSLKRDLENFDRYNIFSSERLAYGSITISDRESVKQ
                     SIINYVKENEDIDTEKLIGDIFPKFTPHLFISHKSQDAGLAIKLANILFEKYDILSFID
                     SQVWHHINDIQKILDEEFSKKSEGLYDYRKSNIVSSNIFAMLSSSLYRTIDDSDGFIFI
                     DRATKPNTARITHSTISQINTESPWIYLENIYSNLIRKKSHNRSQIILAKAGLEKLAED
                     VSVSTESRKEPKFTYTAESVDSRTIQTIAKVLDTPYDKREKHPLEGLDFVYSLLS"
                     /name="hypothetical protein"
     gene            3121..3552
                     /locus_tag="JLF42_RS26130"
                     /old_locus_tag="STW0522ENT66_P60040"
     CDS             3121..3552
                     /locus_tag="JLF42_RS26130"
                     /old_locus_tag="STW0522ENT66_P60040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_032623639.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPYADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICIAMDYSKNGRLSSSDDFPVSRSA
                     DNHELTHHIELMKKELKENQEYLDESFKRLSAELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(4081..4296)
                     /locus_tag="JLF42_RS26135"
                     /old_locus_tag="STW0522ENT66_P60060"
     CDS             complement(4081..4296)
                     /locus_tag="JLF42_RS26135"
                     /old_locus_tag="STW0522ENT66_P60060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4303..4794)
                     /locus_tag="JLF42_RS26140"
                     /old_locus_tag="STW0522ENT66_P60070"
     CDS             complement(4303..4794)
                     /locus_tag="JLF42_RS26140"
                     /old_locus_tag="STW0522ENT66_P60070"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_200925864.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESNDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            5045..5290
                     /locus_tag="JLF42_RS26865"
     CDS             5045..5290
                     /locus_tag="JLF42_RS26865"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_322091021.1"
                     /translation="MQPQSAVHDSLPGIDARPVGAVVVRLEPPAGQQLGIGDQKVQLQT
                     PLVRVLHPQNTVLIFIESGHQNPLEAGHQFFPLAGR"
                     /name="hypothetical protein"
     Typing          complement(539..712)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.143"
                     /coverage="100.0"
                     /evalue="2.63e-66"
     Typing          693..1181
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.538"
                     /coverage="81.0"
                     /evalue="0.0"
     Typing          complement(109..425)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="2.72e-60"
     Typing          complement(3990..5368)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.074"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 ccggcttccc ctgcaggacg gtggcgcctt cacgctgccg gtctttaccc agcagatagt
       61 ccaccggccc tgcgccaccg ccgcgaccgc gtggatgaaa tttaacaatc atcgtccgtt
      121 cctttctcaa gaacggcgtg tcgcagccgc tcaagcccgg catcgatggc catcagcgcc
      181 gccacaatct gtaccctgtc atggccggtg ccgccgccgg cgttaacacg gcgggctatc
      241 tggttcaggt tattgcccat gccggcaagc tggcgcagca gcgccggcga gatagagggg
      301 agctttccgg tgcgcgcagg cttttcgtcc aggcaggtct gccgcatcca cgccgccagc
      361 tgcctgccgt cacagcgctc aagcagccgc cggtgctcgc cctccgtcac ccacatggtg
      421 agcatcttgt tccgtttctc tgacaaaccg gcctcctgat aacgccggga cgggcgggaa
      481 aggctttccc ggtcggcgcg gggttggaca agccgcaggc gcgtcaggct tttagcgggt
      541 ttcggggcgc agccctgaac cagtcatgta gcgctagcgg agtgtatact ggcttactat
      601 gttggcacgg atgcggaaat cagtgaagtg cttcatgtgg aaggagaaaa aaggtggcac
      661 tagcgcgtaa gcagaatatg cgataccgga gatattccgc ttcctcgctc actgactcgc
      721 tgcgctcggt cgttcggctg cggcgagcgg taccggctta ctggcggggc agaaatttcc
      781 tggaagatgc caggacgaaa cttaacgggg aagcgataag gccgcggcga agccgttttt
      841 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
      901 aaacccgaca ggacttaaag ataccaggcg tttccccctg gtggctccct cgtgcgctct
      961 cctgttcctg cctttcggtg taccggtgtc attccgctgt tatggccgcg gtttctcatt
     1021 ccacgcctga cactcggttc cgggaaggca gttcgctcca agctggactg tatgcacgaa
     1081 ccccccgttc agtccgactg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1141 gaaagacacg acaaagcgcc actggcagca gccactggta acaggattag cagagcgagt
     1201 taatgtagat ggtgctatag agttcttgaa gtggtggcct gactacggct acactggaag
     1261 gacagtattt ggtatctgcg ctctgctgaa gccagttacc aggttaagca gttccccaac
     1321 tgacttaacc ttcgaaaaac cacctcccca ggtggttttt tcgttttcag agcaagagat
     1381 tacgacttaa cacctaaaat ctcaagagat cctcaataca aattgatgta aacttcagaa
     1441 gaaccaacgt tctaactcca aaaccctaaa acttttaaaa agccgtaaga aaaaccatta
     1501 agacaaagga aaaaaatgaa taacgaaagt atggaagcta agatagcttc actaaacaat
     1561 atacaatcca tcataaatag attggctgaa acatcttcaa agctaaaggc aacttatata
     1621 acaataatat cagcagtgtt gaccattgtt attgcttacg taatacctca aaaaccagtt
     1681 gcgtcagaaa acttcacaac aaatgaaaag ctttattcga tcttgatatt tctcataata
     1741 acattagcgt tcatgcttat tgattcttat tatttaagat atgaaagagc aatgcgtaag
     1801 gtttatgatt taaaggctga agataaaaaa gactcagacg gaaaaataaa ttactttgaa
     1861 attagcaaca cattcaacta cgtacttaaa aaccaaaaca taaaatcaag accaagccgc
     1921 tctcaattca tattctatgt aattccgttt ttagctatag cattttctat agcagtatta
     1981 gcgacgaggt aaaaatgcat aagaaattcc acattgagtt agattccctc aagagagatc
     2041 tagagaactt tgacagatat aatatcttca gttcagagag acttgcttat ggtagcatta
     2101 ccataagcga tagggaaagt gtcaaacaaa gcatcataaa ctacgtgaaa gaaaatgaag
     2161 acattgacac agagaagttg ataggtgaca tatttcctaa attcacacct catttgttca
     2221 tatcacacaa aagtcaggat gccggactag ctataaagct agcaaacata cttttcgaga
     2281 aatatgacat tctttcattc atagactctc aagtttggca tcatataaat gatattcaga
     2341 agatacttga tgaagagttc tcaaagaaaa gtgaaggtct atatgactat aggaaaagca
     2401 atatagtctc cagtaatata tttgcgatgt tatcatcatc tttgtataga accatagatg
     2461 attcagatgg cttcattttc attgacagag caacaaagcc aaacacagca agaataaccc
     2521 actctacaat atcacaaata aatacagagt caccgtggat ctatttggaa aacatataca
     2581 gtaatctaat cagaaaaaaa agccacaacc gatcgcaaat tatactcgcc aaggctggat
     2641 tggaaaaact agcagaggat gtttcggtat cgactgagag tagaaaagag cctaaattta
     2701 catatacagc ggaaagtgta gattcgagaa caattcagac gattgctaaa gtacttgata
     2761 caccatacga caaaagagaa aagcatcctc tagagggact ggattttgtt tatagcctat
     2821 taagttgatt aggaaaggtc gacccccggt cggctttttc gcatctgcag aaccactaca
     2881 atttaccata acatccgtat tgcgccctct ggatggagca ccatttagag ctttatccac
     2941 actcttcaac gcccgctatt agccgtatat accagttaac catctcagac tcatccatca
     3001 aaaaatcaat caaaacaatg aaataaccta gaattctcat tttgttacat ttagtaaatt
     3061 tactaattag taaaacaata taatctacaa atcaacaaat aaatgagaga gacatagaga
     3121 atgaaaatcc ccgcccacct gaaacataag ccaattattc aagtagagaa ttatgatcgc
     3181 attgatggac catatgccga cgacactgat gcaatggggt tatctgttgg tatcgcacaa
     3241 tggaacaccc cgggatggac agagctttcg gcaaaggttt ggcgaaatac cggcgagaag
     3301 tggtcccgtc agtctgagga gcttccactt caccgagtaa tagaccttgc cactctgatc
     3361 tgcatagcga tggattacag caaaaatggg cggctatcat ccagtgacga cttcccggtc
     3421 agtcgttctg cagacaacca tgaactcact catcacatcg aactaatgaa aaaagagtta
     3481 aaagaaaatc aggaatatct cgacgagagc tttaaacgcc tttcggctga attgaaacgt
     3541 cttggctact aaaaaaagcc gcccatctgg gcggcttttt tgtgtctgca gcaccgccgt
     3601 atttaccata acatctgttg ggcgcaccgc tgaaacggga ttggctgcgt ttccggtgcc
     3661 agacgcgaga aagtggcgac agacataacg aaaaaacacc agaaacgcag ccgctgaata
     3721 ttacactggt gcatagtcat gcagccagat gcatggatgt taaagtcaca ctaaaatccg
     3781 cgccaatgcc ttaaaaaccg acaaatgctt ctccagccag tcccacagct ccgccgccag
     3841 atacaggcag ttaagcaatg attcaataag atttaacagc ttcatttccc gctcctgaat
     3901 aagtcgtgac ctttttgatc acgaacctat ttagcgggag aaataagagc ccctcttcgt
     3961 gcgggttgtc agctgcctgg atggtttaaa gtgtcggccc gcggtaggtt ttctgcggct
     4021 ggcgctccag ctccagtgtt ttctgatact cattctccct ctgccggagc tcgatggcat
     4081 tcagttcccg gacaagcggc gcagctgctc gcttaaactc agcacctgcg tgctcaagtc
     4141 cgacacgcgc tcgttcagca ccgcgttctc ccgttgcata agcccacaca tgctccgcca
     4201 ttccgcgaag gcgctctccc attcgtccag cctttgcgag tagtcctgct gtagctgctc
     4261 taatgcgctc agcaactgct tttccagctc tgtcattttc cgctactcct gtttcaggcg
     4321 gatccaggtc gtcccgtcgt aggggatggc ttcggcttgc atccccggcg gcaggataag
     4381 gaacctccgg ccgtcgttgc tctcctgata gcgtactccc cacgtccggg cggtcatttt
     4441 cgccagggta tcttcctgct gacgaagatg tgtgtaattg tcggttatct gccgtccctg
     4501 ccaccacaga atgctcccgc tcgtggcgat cagcagcgct gacaccatca ggatggtgag
     4561 ccatgtcctc ccggccgtca gcaacacgct ccgccggttt ttctccatcg cctcgctcat
     4621 actctgctcg tgggcgctga tggcgtcgct gattctcctc gcgcttgcgc tcagttccgc
     4681 tctgacagac tgctcgtgct cgctgaatgc ggctttcagc atctcgccgg tactcttctg
     4741 ctgcgctttc gattgctgct ctaagtcctt cgccagcgtt aaaagactgc tcatagatgg
     4801 ctcccttcag tcggatgttt cgccccccgt cagggtcggc aatgctgata ctgcttttcg
     4861 tggtacgcac cacctcaaaa cctgcacctt caagagcctc agtgacgtcc tgacggcttt
     4921 tcagctcccc ggacgaggct aagctcagca gtccccgggt aatggcttct gcggcttcct
     4981 gcgtcgtttt cggcagcccg gacggcgtga ccagcgcccg gcggttttcc ggcgcgttcg
     5041 ggtcgtgcag ccccagtctg ccgttcacga tagtctgcca ggcatcgatg cgcggccggt
     5101 cggcgcggtc gtagtaaggc tggagccgcc tgccggtcag cagctcggta ttggggatca
     5161 gaaagttcag ctccagacgc cccttgtccg cgtgctccac ccacagaata ctgtactgat
     5221 ctttatcgag tccgggcatc agaacccgct cgaagctggc catcagtttt tcccgctggc
     5281 cgggaggtaa atccgcttcc gcaaacgaca ggacgccgga ggtgtatttc ttaacgtaag
     5341 gcgacgcgtc aatcagctcc cggacttctt
//
