LOCUS       NZ_AP022468            11238 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter roggenkampii strain STW0522-66 plasmid pSTW0522-66-3,
            complete sequence.
ACCESSION   NZ_AP022468
VERSION     NZ_AP022468.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195906
            Sequence Read Archive: DRR199711
            Assembly: GCF_015136315.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter roggenkampii str.
            STW0522-66 isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11238)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 513x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:28:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,279
            CDSs (total)                      :: 5,169
            Genes (coding)                    :: 5,025
            CDSs (with protein)               :: 5,025
            Genes (RNA)                       :: 110
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 47 of 144
            Pseudo Genes (incomplete)         :: 99 of 144
            Pseudo Genes (internal stop)      :: 23 of 144
            Pseudo Genes (multiple problems)  :: 24 of 144
            Pseudo Genes (short protein)      :: 1 of 144
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022468.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="STW0522-66"
                     /isolation_source="sewage"
                     /db_xref="taxon:1812935"
                     /plasmid="pSTW0522-66-3"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            complement(102..569)
                     /locus_tag="JLF42_RS25935"
                     /old_locus_tag="STW0522ENT66_P30010"
     CDS             complement(102..569)
                     /locus_tag="JLF42_RS25935"
                     /old_locus_tag="STW0522ENT66_P30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064186840.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATSATVARTMGIPPRTVRRWASEKA
                     AAPSNVLSIEEMRKRAAAAVEATPQAAIRRLKNHFVQQQFDLLQRHAKDLQALRSASLQ
                     AMLEKDATMVKAISGLMTALLKAQERERLIYEIKPGTEADIMREGMNRKQQ"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(820..1005)
                     /locus_tag="JLF42_RS25940"
                     /old_locus_tag="STW0522ENT66_P30020"
     CDS             complement(820..1005)
                     /locus_tag="JLF42_RS25940"
                     /old_locus_tag="STW0522ENT66_P30020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200925859.1"
                     /translation="MINQAATPRYPGLSIEHGEIRQFGKPRHCAANRKGQYQTTLRDQA
                     GRYRTVSIISLYAWEA"
                     /name="hypothetical protein"
     gene            complement(1115..1690)
                     /locus_tag="JLF42_RS25945"
                     /old_locus_tag="STW0522ENT66_P30030"
     CDS             complement(1115..1690)
                     /locus_tag="JLF42_RS25945"
                     /old_locus_tag="STW0522ENT66_P30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151785.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_101998997.1"
                     /translation="MSRVFGYGRVSTGEQTVAGQKKAILDRYPQATFHADEGISGTVPA
                     TQRDKFKAILEFAAPGDSIVVVAIDRIGRNARSVMEVCEELEKRQVSLISLREGFDFST
                     PTGKMMLTMVAAFAEMELATLKERTKAGMAAAAAAGRHAGRPTKQTPENVGAVIMKHDA
                     GVSAARIARDLGISRGTVNRILQKHAKA"
                     /name="recombinase family protein"
     gene            1941..2600
                     /locus_tag="JLF42_RS25950"
                     /old_locus_tag="STW0522ENT66_P30040"
     CDS             1941..2600
                     /locus_tag="JLF42_RS25950"
                     /old_locus_tag="STW0522ENT66_P30040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061548402.1"
                     /translation="MTEIKKESADGAWLRSTLAELAGDAFPVYDLPSLQVDTNSSIQLS
                     ALADRQAAREMRQAASDVEARRLDAARVVEGLKTEAERLRGLIADGKAALRAGEPVSPD
                     AGVASFLLPDIEAELVVAEAAEADVARERDTLLQDADRRDAAAALALFNWAHSVRVQRI
                     ELLLKLAMDEATTLAETDGGRGLYRTVIAPDRRLNQIFATQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            2600..3172
                     /locus_tag="JLF42_RS25955"
                     /old_locus_tag="STW0522ENT66_P30050"
     CDS             2600..3172
                     /locus_tag="JLF42_RS25955"
                     /old_locus_tag="STW0522ENT66_P30050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200925860.1"
                     /translation="MSAFKLLCDRLRNEATLAKSDGAGAFKKQSLDPDLKGGKKGKKPA
                     GELEEDEAIASLHRKRMNDVKGCKTLTKSTGADDFGLPPLSSVVETVVETGGAAQTFAP
                     IWAPVAYPTEAVHRARAQSIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAARAP
                     AFAEVRRQVHAGGMTLSPAARGIDWNA"
                     /name="hypothetical protein"
     gene            3165..3527
                     /locus_tag="JLF42_RS25960"
                     /old_locus_tag="STW0522ENT66_P30060"
     CDS             3165..3527
                     /locus_tag="JLF42_RS25960"
                     /old_locus_tag="STW0522ENT66_P30060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200925861.1"
                     /translation="MRELPRHLQGEVLMPLSSRITRSQRLELIWLLPVLRLHPGDRSVT
                     ERRALGRFRGSLAAGGQTDAFVRSDASLYALGLRFRRERTRELVASDPDVAAAGEALEQ
                     RANGVNLSYTFGGGDE"
                     /name="hypothetical protein"
     gene            3520..3705
                     /locus_tag="JLF42_RS25965"
     CDS             3520..3705
                     /locus_tag="JLF42_RS25965"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151322523.1"
                     /translation="MSEFAVSRLVIPEVVIPDGVIAARRARGQAQRVAAARRAAERRAA
                     PTWRWVRRELPRSTGR"
                     /name="hypothetical protein"
     gene            complement(3725..4366)
                     /locus_tag="JLF42_RS25970"
                     /old_locus_tag="STW0522ENT66_P30070"
     CDS             complement(3725..4366)
                     /locus_tag="JLF42_RS25970"
                     /old_locus_tag="STW0522ENT66_P30070"
                     /inference="COORDINATES: protein motif:HMM:NF016255.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_200925862.1"
                     /translation="MTEPTRPILTIKRRNRTVYVPPEKATKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGTELDMPPEADRKAVRRILGIVAGTSSYQQC
                     LIDQCMRYDLDGNPTVAVTPEEAEQAKTRLTEIREFRRKARQEKDKKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(4363..5652)
                     /locus_tag="JLF42_RS25975"
                     /old_locus_tag="STW0522ENT66_P30080"
     CDS             complement(4363..5652)
                     /locus_tag="JLF42_RS25975"
                     /old_locus_tag="STW0522ENT66_P30080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_145062810.1"
                     /translation="MNDDNDTNESTQPNPAALERVSATEKFPEQLPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCHEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTASVCSDCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFTQNETRSLAKIPV
                     ILPQREQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLTLSVLAAVGRWDYINDI
                     IRRIDRDRGDAATLIALADHIQTNRPERDL"
                     /name="hypothetical protein"
     gene            6008..6217
                     /locus_tag="JLF42_RS25980"
     CDS             6008..6217
                     /locus_tag="JLF42_RS25980"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186834.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGNPEIKRFVADQRRPGRAADGVR"
                     /name="hypothetical protein"
     gene            6204..7376
                     /locus_tag="JLF42_RS25985"
                     /old_locus_tag="STW0522ENT66_P30090"
     CDS             6204..7376
                     /locus_tag="JLF42_RS25985"
                     /old_locus_tag="STW0522ENT66_P30090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172743605.1"
                     /translation="MAFVDFDTTHLKEDPGLFWRAVSAAGAPADRDEWVRLCLALGCWA
                     SLCGEDGLAAIEAWSTRHGRGENRHRQQYRSFLKGDLDTFAAQAFLNFSGVEWRNGRAS
                     VQSDVDRAERLRLSREATAKAKAERDKKGDAAARAAVKLLAKGGDPASTPYWRKKTALA
                     AAGGVQLPALSTDLGVGGMFRSFDPRAYWSDCLLIPVYKFDAARQLYVDGLEAICGRCS
                     AEGSQLTGSKFSPAGTRKQGGFYPMPGFMDVLDGSPHAPYSPLFVCEGFATAFALHHLS
                     RGKVAVCAAFSIAGFGSCVASLRRFWPDVEVMLVPDAGEAYRLAHKAAYGDDREPVPTI
                     PGASVVSFDGLNRFEPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERG"
                     /name="hypothetical protein"
     gene            7633..8091
                     /locus_tag="JLF42_RS25990"
                     /old_locus_tag="STW0522ENT66_P30100"
     CDS             7633..8091
                     /locus_tag="JLF42_RS25990"
                     /old_locus_tag="STW0522ENT66_P30100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200925856.1"
                     /translation="MKSKTELRAAATARALEVIASEMGGWSLDGFAHGSLPNFSPLPRQ
                     QTQDGSVVLERPPFDYTWAGTAAFTDRANRALQVKLPASRERNYIWLCAVEREAVATAL
                     IVESFNVTGCAAFAGLPPVDGMVLLTMDEADVELIRAAMLPWLDAAAA"
                     /name="hypothetical protein"
     gene            complement(8109..8450)
                     /locus_tag="JLF42_RS25995"
                     /old_locus_tag="STW0522ENT66_P30110"
     CDS             complement(8109..8450)
                     /locus_tag="JLF42_RS25995"
                     /old_locus_tag="STW0522ENT66_P30110"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200925857.1"
                     /translation="MDTKLLTAIEALRSEVRALAGGGKDARQFAPQTARQSAPQNARQI
                     VPRGADPRFQAVSDEADQLIVSMTGENATLPAIAQALNAAGHTTATGQEWNTSRVRDYR
                     HKLRERGLI"
                     /name="hypothetical protein"
     gene            9106..10677
                     /locus_tag="JLF42_RS26000"
                     /old_locus_tag="STW0522ENT66_P30120"
     CDS             9106..10677
                     /locus_tag="JLF42_RS26000"
                     /old_locus_tag="STW0522ENT66_P30120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200925858.1"
                     /translation="MSNHEENSGAAADNVTPITAAPSPLSLKLGDALFSVLSVSADWSG
                     DYRAQFELYGLNVKAINSAVGTAVWHAGKGRFLSVLNGSLTEFDKGDGMKLLEDSCGKF
                     WHRTDAFIARLTDLKIKTDDKVTKACIDMARAVRQAVAEFIMLRRQVAVVRLDVDMFAT
                     APRVELVGETVTFVRPHAPYPVANADSDVVADWLVHFPQCHEFLDALVAARFASSRKNA
                     YLFFRAQSDWGKGLLFGAGGVLSRLGATVELSEGELLNILSGANSGVTASHFMGALALI
                     VNECTRVTKKHFRLEESLALTPKYLTTQCVNLYMKIFTSADPIPGLSDTNGIDPQVANR
                     FSMLDLQGDIKTRPLFLSDKGRYVDSLTSFFAAELNARVASYKAKGFDAARRDADHVLA
                     AFTAAHGLAKTAGLITDAYDEIRDDWVAFVHSRVADGHPDFLTFESSNGLVLRSPAGCW
                     SRFLEWYVDKSEPLRRARLLHDRDLIIGVAKAERDVTTGKVTKGVILPFVSGSMPDNVH
                     RFRYKN"
                     /name="hypothetical protein"
ORIGIN
        1 gcggtaacaa ttgcgtaacg tgcggatccg cgtattccgt gggctcccgg gttttggcgg
       61 tcacaaaaac ggtcacatgg cggtcatttc ggtcatggcg gtcattgttg tttgcggttc
      121 atgccctcgc gcatgatgtc ggcctcggtg cccggcttga tctcgtagat gaggcgttcg
      181 cgttcctgcg ctttgagcag ggcggtcatg aggccgctga tcgctttgac catggtggcg
      241 tccttctcca gcatggcctg taacgatgcg ctgcggaggg cttgcaagtc ctttgcgtgg
      301 cgctgtagca ggtcaaattg ctgctgtaca aagtggtttt taaggcgtcg gatcgcggcc
      361 tgtggtgtgg cctcgacggc ggcggcggcg cgtttccgca tctcctcgat gctgaggacg
      421 ttgctgggtg ctgcggcctt ctcgctcgcc catcggcgaa cggtgcgggg tgggatcccc
      481 atggtgcgtg cgacggtggc actggttgcg ccgtcctgct ccagtagcct gagcgcctgg
      541 gctttctgtt cggtggtgta tttcttcatg gttcctgctc cggttggttt ctctcccgta
      601 ttgaaacgtc acgcgctgaa aatggcgggg gtttttgggg ggtacacggc gtttgtgcgt
      661 gcgtggtcgt gcggttgggt gtttttgtgg ctgaggcgct tagaatggct ctggtgcgtt
      721 ctggtgaatg gggcgcttcc gcgcccctgt aattggttcg taatggtgta aggcttttgc
      781 tattgcttgt tgcttacacc tgtaaggctt tcgttatggt cacgcctccc atgcgtaaag
      841 gctgatgatg ctgacggtgc ggtatcgccc tgcctggtcg cggagggtgg tctggtactg
      901 gcctttgcgg ttggcggcgc aatggcgagg cttgccgaac tggcggatct cgccgtgttc
      961 aatgctcagg ccgggatagc gcggtgttgc tgcttggttt atcatggttc tgttccttgt
     1021 tgggaatggt gcccgggtct gctcctcatg cgttccccgg gctgggttgc tgcggtgtag
     1081 gcggttggag ccaactacgc cgctgcgctc tttcttacgc tttcgcgtgc ttctgtaaaa
     1141 ttctgttcac cgttcccctg ctgatgccta ggtcgcgtgc tatgcgtgcg gcgcttactc
     1201 ctgcgtcgtg cttcatgatc accgctccga cgttctccgg cgtctgtttg gtcggtcgtc
     1261 ctgcgtggcg tcctgcggct gcggctgctg ccatccctgc ctttgtgcgc tccttgaggg
     1321 tggcgagttc catctctgcg aaggcagcga ccatggtgag catcatcttc ccggtcgggg
     1381 tgctgaagtc gaaaccttcg cggaggctga tcaggctgac ctgccgcttc tccagctcct
     1441 cgcagacctc catgaccgat ctggcgttgc gtccgatgcg gtcgatcgcg accaccacga
     1501 tgctgtcgcc tggtgcggca aactccagga tggctttgaa cttgtcgcgc tgggtggcgg
     1561 gtaccgttcc gctgatgccc tcgtcggcgt ggaaggtggc ttgtgggtag cgatcgagga
     1621 tcgctttctt ctgtccggct actgtctgtt ctccggtgct gacgcgcccg tacccgaata
     1681 ctctgctcat gctgtctcct gttgtacaaa aaaggttgtt ttgggatatt tgaccactta
     1741 tgagcaaaaa gcaagcgcaa aaaactgtaa aagcctagag ccacgggggc tgcgcagttt
     1801 cgctcctttt tggacgtgat caccccgcgc tgggcgagag ccctgcgccc tctgcgttga
     1861 tgtctgcgct gcgtcgtgac ggcatgctgt ctgagcggcg tttcttgccg ctttgctgat
     1921 aacttgctga gaaccaaacc atgaccgaaa ttaagaaaga gagcgccgac ggcgcttggc
     1981 tacgctctac ccttgccgaa ttggcgggtg atgcgttccc tgtgtatgac ctcccgtccc
     2041 tgcaggtaga cacgaactcc tccattcagc tctcggcgct cgccgatcgt caggctgcgc
     2101 gtgaaatgcg acaggccgct tccgacgtgg aagcgcgacg gctggacgcc gctcgagtgg
     2161 tggaagggct caagaccgag gccgaacggc tgcggggctt gatcgccgac ggcaaggctg
     2221 cgctgcgtgc tggcgagccg gtgagccccg acgcgggtgt tgcgtcgttc ctgctgccgg
     2281 acatcgaggc cgagctggtt gttgctgagg ctgctgaggc cgacgtggcc cgggagcgtg
     2341 acaccctgct gcaggatgcc gatcgccgtg acgcggcggc tgcgctggcg ctgttcaact
     2401 gggcgcactc cgttcgcgtg cagcgtatcg agctgcttct caagctggcg atggacgagg
     2461 cgacgacgct cgctgaaacc gacggcgggc gcggcctgta ccgtaccgtg atcgcgcctg
     2521 accgccgtct taaccaaatt ttcgccacgc agggcgctat tgaaatcctg cgccgtaacc
     2581 gtggcatgga gggtgtgtaa tgtctgcttt caaactcctg tgtgaccggc ttcgcaatga
     2641 ggcgacgctg gcaaaatctg acggggccgg cgctttcaaa aagcaatctc tggatcccga
     2701 cctgaagggc ggcaagaagg gcaaaaagcc cgccggggag ctggaggagg acgaggcgat
     2761 cgcgtctctc caccgtaagc gcatgaacga cgttaaaggt tgcaagacgc tgaccaaatc
     2821 caccggcgct gatgactttg gcctccctcc gctgtcgtcg gtggtcgaga cagtggtgga
     2881 gacaggcggc gcagcgcaga cgttcgcgcc gatctgggct ccggtggcgt atccgaccga
     2941 agcggttcac cgtgcgcgtg cgcagtccat ccaggacgcg gcgttcctga acgagatgaa
     3001 agcggcgggt gtggcaccgg ctgacctggt gttacttgac ggcgtgttga cgtcctctcg
     3061 tgggatgcca gacgcggcgc gtgctccggc gtttgccgag gtgcgccgtc aggttcatgc
     3121 gggcggcatg acgctgtcac cggctgcgcg tggtattgac tggaatgcgt gagctgccgc
     3181 gtcacctgca gggcgaggtg ttgatgccgc tgtccagcag gatcacgcgc tcgcaacggc
     3241 tggagctgat ctggctgttg cccgttctgc ggctccaccc gggcgatcga tcggtcactg
     3301 agcggcgggc gcttggcagg ttccggggat cgctcgctgc gggtggccag acggatgctt
     3361 tcgtccgttc ggatgcgtcg ctgtatgcgc tggggctgcg gttccggcgt gagcgcaccc
     3421 gggagctggt cgcttctgat cctgatgttg ctgcggctgg cgaggcgctg gagcagcgtg
     3481 caaacggtgt gaacctgtcc tatacgttcg ggggtggcga tgagtgaatt tgctgtctcg
     3541 cgcctggtca ttcctgaggt ggtgatcccc gatggggtca ttgctgcccg acgggcaagg
     3601 ggacaggctc aacgtgtcgc agctgctcgc cgtgctgcag agcgtcgtgc tgcgcctacg
     3661 tggcgctggg tgagacggga actgccccgg tcgacagggc ggtgaaacgg tgagacgggg
     3721 cgcgttatgc gccctttttt ttgtccttct cctggcgtgc cttgcgtcgg aattcccgga
     3781 tctctgtcag gcgggttttg gcctgttcgg cctcctctgg cgtgacggcg acggtggggt
     3841 tgccgtcgag gtcgtagcgc atgcactggt ctatgaggca ctgctggtac gagctggtac
     3901 ccgcgacaat gcccaggatg cgacggacgg ctttgcggtc tgcctctggc ggcatgtcca
     3961 gctctgtgcc tcgtgtcagg atctgctcga tgatgccctt tgccatgggg cgacggtcaa
     4021 ctccccgggt cagtaagtca ggccagtgct ctttcaggaa tgctttggtc acccggttgg
     4081 ccagcgcctt tttggacttg ttggcgatcg cgatgtcctg ctggcggcgg tcgttctcct
     4141 cctgtctgcg gatcccctcg gcgacctgct cgggcgtctt tttcggcttt gccgctgtgg
     4201 gcttctgtgc gggcttctgc ggggctttcg cttttggctg taccttcgcc ttgctggtag
     4261 tccgttcggc tggctgtggc tcagatttcg tggccttttc cggcgggacg tagaccgtgc
     4321 ggttgcgtcg cttgatggtc aggatcgggc gggtgggttc ggtcataaat ctctctcagg
     4381 tctgttggtc tggatgtggt cggcaagggc gatcagggtg gcggcgtcgc cgcggtcccg
     4441 atcgatgcgc cggatgatgt cgttgatgta gtcccagcgc ccgacggcgg caaggacgga
     4501 aagggtgagg cggttgaacc gactctctgg tacccggtcg agggtcaggg ggatgcgctt
     4561 gcgggggcgt cctggaccgc gccctgtccc tggtcttttg agcagctgcg gctgctccct
     4621 ctgcggcaaa ataacgggga tcttcgccag gctgcgcgtc tcgttctgcg tgaactcggg
     4681 caccgcgatg tgctcgtctg tccatgtcct gcctccgggc ctggctgatg cgctggagtc
     4741 gcaggacggg caggagacga cgatctcccg tcgcggcttg cccttgaccg gtgccttgct
     4801 gatcaccagg ggcgcggctt tgcaatcgga gcagaccgac gctgtccaga ccgcgttgtc
     4861 tggcagggtg gcgttccggt actggtgcgc ggcgtcatgc ggtgtctgga agctgaccgt
     4921 ctgaaacgtg ggggcgacgc tgaggggtgc cgcccatggc tggcacctgg gacaagcaag
     4981 ttccacctct ccggcgtgcc cgtcgccacc gttccagcgc aggggctcgc ggtgttcgtg
     5041 gcagtagtac acgaaaccgt cgcggcgtgt ttgcccgacc gggcctggtc ggcgatcgtg
     5101 gaaccgtgcg cccgggttgg ctcgcatggc gcggacgagc gcctcgccct ggtggatgcc
     5161 gtaccagacc tgccagccat cgccgatgcc gatcaggaac ggcgagacga acggtggggt
     5221 gcggttgact ctcccgcgtg cgttggctcc gatggtggct tcgcgttcgc ggttccagca
     5281 ggagacgcac aggctcgaac cgggcttatt gggggcgatc aggcggttgt ctttccggct
     5341 gcaacggggg cagagcttac gggtgtctgc cggggtggtc agtggtgcgt cagggggctg
     5401 ttcggtaccg acccaaagcg cgaggccagt ggggcaccgg tggcactcga ggtgggggtt
     5461 ttcaatcgtg cgatcgaggt ggctctgctc ataaatctgt cggcatgcct cgacccggag
     5521 ggagcacccg ccggggcagg tgacgaacaa aacgccgggg agctgctcgg ggaatttttc
     5581 agtcgcgctt acgcgttcaa gtgctgccgg gttcggctgc gtcgattcgt tcgtgtcgtt
     5641 atcgtcgttc actgctcccc ctgcgtcgaa atggtcgcca gacagcgcca gcactggctg
     5701 tgcgtctttt ttagtatctc acaaaaaacg ccactttggc gttaattgtg ttacgcttgt
     5761 cttactgatt cctgacgagc agttgggaaa catgaaaccg gatcgcccgg tgccgctttc
     5821 cctcctcccc aacgaggcgg ggttgtaacc gaggatgcgc gcttcgtcct tggcaggtgc
     5881 gagtaagcga gaagggctgc tgtcctgccg ctatgcggta agtcctcaag ggcggcttta
     5941 tcgataatcg tcactcccgc cccccgatgc ccgttaagct gaggggggca cctatctggt
     6001 actcccaatg aaaaaacgaa tctataccgc tgacctcgct gcgctgctga acgtgtcgct
     6061 gcgcacgctc tcgcggctaa gttcgcgggg gatcgtgccg actccggcag ggcgctccct
     6121 ccgtggcggg cattactggc tggctggcaa tcccgagatc aaacgcttcg ttgccgacca
     6181 gcgtcgtccg gggagggctg ctgatggcgt tcgttgattt cgacactacc catctgaaag
     6241 aggatccggg cctgttctgg cgtgccgtat cggctgcggg tgctccggct gatcgagacg
     6301 aatgggtgcg tctctgcctg gctcttggtt gctgggcgtc tctctgcggc gaggacgggc
     6361 tggcagctat cgaggcctgg tccactcgac atgggcgagg cgagaatcgc catcgccagc
     6421 agtacaggtc gtttttaaaa ggcgacctgg atacattcgc cgctcaggct ttcctcaatt
     6481 tctccggcgt cgaatggcgc aacggtcgcg cttctgtcca gtccgacgtc gatcgcgctg
     6541 aacggctgcg gttgtcccgc gaggccacag cgaaggcaaa ggctgaacgc gacaagaagg
     6601 gtgatgctgc tgcgcgtgct gcggtcaagc tgctggcgaa gggaggggat cctgcgtcta
     6661 ccccatactg gcgcaaaaag accgctctgg cggctgcggg tggcgtccag ctgcccgccc
     6721 tcagcacaga tcttggtgtt ggcgggatgt ttcgatcgtt cgatccgcgt gcttactggt
     6781 ctgattgcct cctgattccg gtttacaagt tcgacgccgc ccgtcagctg tatgttgacg
     6841 gcctggaggc catctgcggg cgctgctcgg ctgagggttc ccagctcact ggctcgaagt
     6901 tctctccggc gggtacccgc aagcagggcg gcttctaccc gatgccgggg ttcatggacg
     6961 tgctcgacgg ctccccgcac gcgccctact cgcctctgtt cgtctgtgag gggttcgcta
     7021 cggcgttcgc cctgcaccac ctgagccgtg gcaaggtggc ggtctgcgct gcgttctcga
     7081 tcgccgggtt cggcagctgc gtggcgtcgc tgcgtcggtt ctggcctgat gttgaggtga
     7141 tgctggtacc ggacgctggc gaggcgtacc ggctggcgca taaagcggcc tatggcgacg
     7201 atcgcgagcc ggtcccgaca attccagggg cgtcggtggt gtcgttcgac ggcctgaacc
     7261 gcttcgagcc ccgcgataac ctcgactggt tcgacgtgct ggttgaggag ggcgaggatc
     7321 gggcgcgtga gctgctccgg tatgcggtgc gtcatgcccg gcttgagcgg ggctgatggc
     7381 tgggcttttt tgcgtcggtt tcctcgggag gccgctgacc gtaaagggtc gttttctggc
     7441 tcgtcttttt tgcagtcgct tttgggttgg tgagacaacc tttaagacgc acatccgacc
     7501 gcttccggtt tcctccgttt tagacgccgc tgcggtcgtg acgctatcct gcagctggtt
     7561 tggtttccag cagcacactc aagcccggca ccgttttggt gtcgggcttt ttttcgcccg
     7621 taaggagggc acatgaaatc aaagactgaa ttacgcgctg ccgcgacggc tcgcgccctt
     7681 gaggtgatcg cttctgagat ggggggttgg tcgctcgacg ggtttgctca cggctcgctg
     7741 ccgaacttct ccccgctgcc gcgccagcag acgcaggatg gctctgtggt gctcgaacgc
     7801 ccgccgttcg actacacctg ggcgggtacc gctgcgttta ccgatcgtgc aaaccgtgcg
     7861 ctgcaggtga agctaccggc gtcgcgtgag cgtaactaca tctggctgtg cgcggtcgaa
     7921 agggaggctg tggccaccgc gctgatagtg gagtcgttca acgtgaccgg ctgcgcggcg
     7981 ttcgccgggt tgcctcctgt ggatggcatg gtgctgctga ccatggacga ggccgacgtg
     8041 gagctgatca gggcggcgat gctgccgtgg ctggatgcgg cggcggcgtg agccgtcgcc
     8101 cctctgcgtc aaatcaggcc gcgctcgcgc agcttgtggc ggtagtcccg gacgcggctg
     8161 gtattccact cctgcccggt ggcggtggtg tgcccggctg cgttcagcgc ctgggcgatc
     8221 gcgggcaggg tggcgttctc cccggtcatg ctgacgatga gctggtcggc ctcatcgctg
     8281 acggcctgga agcggggatc ggctccccgg gggactatct gtcgtgcgtt ttgtggtgcg
     8341 ctttgtcgtg ctgtttgtgg tgcaaactgt cgtgcatctt tcccgcctcc ggccagcgcc
     8401 ctgacctctg accgcagcgc ctcgatggcg gtcaaaagtt tggtgtccat cccttccggc
     8461 ttcgtgccga gtccggcgac gagcgcctct attacggcat ctgtgcgagt tactttggtt
     8521 ttctccgctc gctgctcgat gcgctcgatc aggctgacgg gcagtcgaac ggttagggtt
     8581 ctggttttct ctcgcatggg ctggcctctc cgtgtcgtgc gttttgtcgt gctgtttgtc
     8641 ttgcggagtg taatgcgttt tgtcgtgcgc gtcgaatttg acgtagtgca attgtcgtgc
     8701 aggaaggggt agcggcgcgg cccgtggata acttctgtgg ataacaaaat ttcggacttt
     8761 ccggcccgtg gataagtcgc ggaggctgcg cggctctaag atcggcgcgg gatcgcgttt
     8821 atcgctgcgg gatcgctatg tactggcgca gggggtgggt gtaacggtat gatggcgcgg
     8881 ctgaaaaaga aaacccgtcc gggcagacgg gttttcttca aagctgtttt gagtttggag
     8941 ctttcacggc ggtcaccgtg gaaaacacat aataactcat gttgggtgtg aagccagcgt
     9001 gaattatacg cgatcataat gacgcgtgca aagggtgttt tcctcgtaaa gtcccgccgt
     9061 tgtaaggctc cttgagattg gctgttcaat tttgaggacg taaaattgtc aaaccatgaa
     9121 gaaaattccg gcgctgctgc cgacaacgta acgccgatca ccgctgcacc gtcaccgctg
     9181 tcgctaaaat tgggcgatgc gctgttttct gtcttgtcgg tgtctgcgga ctggtctggt
     9241 gactaccgcg ctcaattcga gctgtatggc ctgaatgtaa aagctatcaa ctctgccgtc
     9301 ggcactgccg tctggcacgc tggcaagggg cggttcctct ctgtcctaaa tggctccctg
     9361 accgagttcg ataagggcga cggaatgaag ctgctggagg actcctgcgg caagttctgg
     9421 caccgcaccg atgcgtttat cgcccgcctg actgacctca aaatcaagac cgacgacaag
     9481 gtaaccaagg cgtgtatcga tatggctcgg gctgttcgtc aggcagtggc tgagttcatc
     9541 atgctgcgcc gtcaggtggc agtcgttcgc ctcgacgtgg acatgttcgc cacggctcct
     9601 cgcgtcgaac tggtgggtga aactgtgacg ttcgtccgtc ctcatgctcc ctatccggtg
     9661 gctaatgccg acagtgacgt ggtggctgat tggctggttc atttccctca atgccatgag
     9721 ttcctcgacg cgctggttgc agcccgcttt gcctcaagcc gtaagaatgc ctacctgttt
     9781 ttccgtgcgc agtccgattg gggtaagggg ctgctgtttg gtgctggcgg cgttctgtca
     9841 cgcctggggg cgacggtgga gctgtccgag ggggagctgc tcaacatcct gagcggtgcc
     9901 aactccggtg taacggctag tcacttcatg ggggcgctgg cgctgatcgt caacgaatgc
     9961 accagggtga cgaaaaagca ctttcgtctt gaggagtcgc tggctctgac gccgaagtac
    10021 ctcacgacgc agtgcgtgaa cctgtacatg aaaattttca ccagcgccga tccaatcccc
    10081 ggcctgtccg ataccaatgg cattgacccg caggtggcca accgtttttc tatgctcgat
    10141 ctgcagggcg acatcaagac gcgcccgctg ttcctgtccg acaagggacg ctatgtcgac
    10201 agcctgacgt cgttctttgc cgccgagcta aatgctcggg tggcgtccta taaggcgaag
    10261 ggctttgatg ccgcccgccg cgatgctgac catgttctgg ctgctttcac cgccgcgcat
    10321 gggttagcga aaaccgccgg gctgatcacc gatgcgtatg acgaaatccg cgacgactgg
    10381 gttgcgttcg ttcatagccg tgttgccgat gggcaccctg acttcctcac cttcgagtcg
    10441 tctaacgggc tcgttctccg ctcacctgct ggatgctgga gtcggttcct cgagtggtac
    10501 gtggacaaaa gcgaaccact gcgccgtgct cgcctgctgc atgaccgcga cctgatcatt
    10561 ggggtggcaa aggctgaaag ggatgtaacc accggcaaag tgaccaaggg ggtgatcctc
    10621 cccttcgttt ccggttcgat gcctgacaac gttcaccggt tcaggtacaa aaattaaaca
    10681 ataacccgct tcggcgggtt tttttacgcc ttgtaaccgg cttgcaaccg tctgtaaccg
    10741 accttgtaac cgccgcaaac ccgtgccagc tctggctgca actttgtaac cggaaaatac
    10801 cgaacgatcc ctgctatgaa aactgttttc tatttttttc tgataagtct ttctgtttta
    10861 atggttacaa ggttgcattg ttgattttaa aggctttttt gcggttacag tattggttac
    10921 aggcggttac aggcggttac agctcaattc ctgcgcagtt gacgcgccgc agctgcaccc
    10981 tgcgggatgc actttgcttg tcttggtcga tcggtggatg ggttcgtcgt gccggggtgg
    11041 tgggtggtgg tggttagggg gtggatcggg tggttggggt ggcgctgagg gcacgagctg
    11101 tcgctcgcgc tgggtggtcg tctgttggtt ctgcgtgagg gcttgccctc gcgccctctg
    11161 cgttatgggt ggaatgcgta gctgcagctg cgcgtttggt tcgttcgggc atcaaaacgg
    11221 ggcttctcgc gcacgcga
//
