LOCUS       NZ_AP022464             2544 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STW0522-62 plasmid pSTW0522-62-12,
            complete sequence.
ACCESSION   NZ_AP022464
VERSION     NZ_AP022464.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195902
            Sequence Read Archive: DRR199707
            Assembly: GCF_015136055.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STW0522-62
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 550x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,030
            Genes (coding)                    :: 4,914
            CDSs (with protein)               :: 4,914
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 34 of 116
            Pseudo Genes (incomplete)         :: 80 of 116
            Pseudo Genes (internal stop)      :: 22 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            Pseudo Genes (short protein)      :: 1 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022464.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STW0522-62"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTW0522-62-12"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            101..700
                     /locus_tag="JLF39_RS25585"
                     /old_locus_tag="STW0522ENT62_P120010"
     CDS             101..700
                     /locus_tag="JLF39_RS25585"
                     /old_locus_tag="STW0522ENT62_P120010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052685777.1"
                     /translation="MKKKNNIEYLQLNNAENSEDFDIEHKKNPFVFNMEIDTKKKKLTV
                     DRGSELETEEGVFTTTISQIQEVDKEEFLKLFTGQIKFYFDLTQTGFKLFFLIMGIYQK
                     QIGGDEIYLNFETAKEEAEKYDYTISKAVYYRGLKELLEKKMIAKSVKKYIFYINPAIV
                     FNGDRARFVKEIRIKEEEKQREADLKSINERLDRYA"
                     /name="hypothetical protein"
     gene            complement(749..1921)
                     /gene="mobV"
                     /locus_tag="JLF39_RS25590"
                     /old_locus_tag="STW0522ENT62_P120020"
     CDS             complement(749..1921)
                     /gene="mobV"
                     /locus_tag="JLF39_RS25590"
                     /old_locus_tag="STW0522ENT62_P120020"
                     /inference="COORDINATES: protein motif:HMM:NF013258.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_200925428.1"
                     /translation="MAYAILRVEKTKNTSIAGKNSHNMRLRKTHNADPNLKSQNRILIG
                     SGDLRTDINARFQATNVKARNSTSVICNELVLTASPEFFANNKKLDDWIKVQMEYLQSE
                     YGENAINAVLHLDEQTPHIHAFITPIENKNGIYKLNNKSYMKKYETMQDIYFKYNKPLG
                     LIRGVKKEVSNAEYKEVKEFYSDIKNIKNETELEIENNKIEKIRVVETEEKKKLFRDPE
                     VVPKHYTGAEVNRYIRKALKPYKNERKPLIARLNGFKSRLEHSEAELSDLRQNFNRRVQ
                     ERVQQEQKLAVARATAEQEQIIQNKNRKIKDMELEIIEYKRQLKENASISQRYEKYKIN
                     SDVLDLIQEHKPAEFKQLFNAAIAAADAEYSQKQQNNNYTPQNKPKALKL"
                     /name="mobV"
     Typing          complement(750..946)
                     /name="Col(IMGS31)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.85e-99"
     Typing          complement(753..1921)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.487"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctagtttcaa aaatctgtta aaaaatgaga aaacgagtct catcgagtga gaaaaaaccc
       61 gtttaaaaaa tatacaaatt aaactaaaag agaggtgttt atgaaaaaga aaaataatat
      121 agagtattta cagttaaaca atgctgaaaa tagtgaggat tttgatattg aacacaagaa
      181 aaatcctttc gtgttcaata tggaaattga tactaaaaag aaaaagctaa ctgttgatcg
      241 aggttctgaa ttagaaaccg aagaaggagt ttttactaca acaatttcac aaatccaaga
      301 agtagataaa gaggaatttt taaagctgtt tactggtcaa attaaattct atttcgattt
      361 aactcaaaca gggtttaaat tatttttctt gattatgggt atttaccaaa agcaaatcgg
      421 cggtgacgaa atttacttaa atttcgaaac agcaaaggaa gaagctgaaa aatatgatta
      481 tactatttca aaagctgttt attatcgtgg attaaaagaa ctattagaaa agaaaatgat
      541 cgcaaagtct gttaagaaat acatatttta tataaatcct gcaattgtat ttaatggcga
      601 tcgtgcaagg tttgttaaag agatccgaat taaggaagaa gaaaaacaaa gagaagcaga
      661 tttaaaaagc attaatgaaa gattggatcg atatgcttaa aaaaccaaaa cccaacggga
      721 aatttttcac tgtttcccgt tccgggcttt ataattttaa agcctttggc ttattctggg
      781 gtgtgtagtt attattttgc tgtttctgtg aatattcggc atctgctgct gcaatagcag
      841 cattgaagag ttgtttaaat tctgccggtt tatgctcttg tattagatct aaaacatcac
      901 tgttaatttt atatttttca tatctctgag aaattgaagc attttccttt aactgccttt
      961 tgtattctat tatttccaac tccatatctt ttatttttct gtttttattt tgaatgattt
     1021 gttcttgctc agccgtggct ctggccactg ctaacttttg ctcctgttgt acccgttcct
     1081 ggactcttct gttgaagttc tgacggagat cagacaactc agcctctgaa tgctctaaac
     1141 ggctcttaaa cccgtttaga cgagctataa ggggctttcg ttcgttcttg taaggtttta
     1201 gagcctttcg tatgtaacga ttaacctcag ctcctgtgta gtgcttaggc actacctccg
     1261 gatctctaaa taattttttc ttttcttctg tttcaacaac tctaattttt tcaattttat
     1321 tattttctat ttctaattcg gtttcatttt taatgttttt aatatcgctg taaaattctt
     1381 taacttcctt atattctgca ttcgaaacct cttttttaac cccacggatt aaacccaatg
     1441 gcttattgta tttaaaatat atatcctgca ttgtttcgta ttttttcata tacgatttat
     1501 tgtttagttt atatattccg tttttatttt cgattggagt tataaaagcg tgaatatgtg
     1561 gtgtttgctc gtctaaatgt aaaactgcat taattgcatt ttccccatat tcactttgca
     1621 aatattccat ttgaacttta atccaatcat ccaatttttt gttatttgca aaaaattctg
     1681 gggaagctgt taaaactaat tcattacaaa taacagaagt agaattacga gctttaacat
     1741 tggttgcttg aaaccttgca ttaatatcag tccttaaatc accagaaccg attaaaattc
     1801 ggttttggga tttaaggtta ggatctgcat tatgtgtttt cctcaatcgc atattatgag
     1861 aattttttcc agcgattgaa gtattttttg ttttttccac tcttaaaatt gcataagcca
     1921 tatttgaaaa cctcccggta aaagcagtaa ggttttttct ttttggcccc tgccaggctc
     1981 acaccgagat ttctcggtat agtgagtata cctttctgca aaattgaaaa tctataaata
     2041 catctacaat aaaaaaaaca aaagtcaacg gatcaatccc tcgcaagggg aaattaaaat
     2101 tcccccttac tcacgattac caataaaaga aaaaagacag aacgctgagc aagtcaaaat
     2161 tttaattttg gcttgtgaag ggttgaccaa gcgaagcgcg gtagggaaat ctgcgcagat
     2221 tgcttatgta ttgcccggaa cgggaaacgt ctgttgtagc ggtagcgaaa acacatctcc
     2281 cggaaggggg ttttcttttg cgtagcctgg caagttctgc agatagggct gggactaggt
     2341 tgaatcctct tagatcttat atttcttata ttcttaaggg gtctgtaacc cccgtaaaac
     2401 ctaacggtct agtctctcac atgtaaaaaa attctctcct gagagaattt tttctctcct
     2461 gagttactaa tagtctctcc tgagttacta ttagtatcgt ttttttttga aaaaacgaga
     2521 aaatatttag tttagctctc ttga
//
