LOCUS       NZ_AP022463             2689 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STW0522-62 plasmid pSTW0522-62-11,
            complete sequence.
ACCESSION   NZ_AP022463
VERSION     NZ_AP022463.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195902
            Sequence Read Archive: DRR199707
            Assembly: GCF_015136055.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STW0522-62
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 550x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,030
            Genes (coding)                    :: 4,914
            CDSs (with protein)               :: 4,914
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 34 of 116
            Pseudo Genes (incomplete)         :: 80 of 116
            Pseudo Genes (internal stop)      :: 22 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            Pseudo Genes (short protein)      :: 1 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022463.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STW0522-62"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTW0522-62-11"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            102..551
                     /locus_tag="JLF39_RS25915"
                     /old_locus_tag="STW0522ENT62_P110010"
     CDS             102..551
                     /locus_tag="JLF39_RS25915"
                     /old_locus_tag="STW0522ENT62_P110010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_210472981.1"
                     /translation="MKTKSAGNKLATYLQSHSIIRDSSEGVSGKNISFSVSMPIERYYL
                     FKEISNRYNISMSQIVNVALHAFDSELLLSLDRESSFEIARAADDGITSDIGGEPVSYW
                     YSKVLQKAAQEDFARFCEANPDIPEEHLDDAYQSYLAQSAREEIF"
                     /name="hypothetical protein"
     gene            551..841
                     /locus_tag="JLF39_RS25575"
                     /old_locus_tag="STW0522ENT62_P110020"
     CDS             551..841
                     /locus_tag="JLF39_RS25575"
                     /old_locus_tag="STW0522ENT62_P110020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008054160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050136734.1"
                     /translation="MLSMTGQLLNVFENPRGVTKEGREYGGNYKIQVLGAVTLQNGETK
                     NDLVDLTCHDIAAFRPLLGQRISFPIGVMSMGKNQTVFFIPKGGMPEPVGG"
                     /name="hypothetical protein"
     gene            897..1961
                     /locus_tag="JLF39_RS25580"
                     /old_locus_tag="STW0522ENT62_P110030"
     CDS             897..1961
                     /locus_tag="JLF39_RS25580"
                     /old_locus_tag="STW0522ENT62_P110030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01629.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid replication protein, II/X family"
                     /protein_id="WP_200925426.1"
                     /translation="MMIDWLTLRIPCDHSREIAGGAMVSYKDAWDFDAGADWCFTKPLA
                     VVGSHDANVRLKTSGFSNEGQSGTELHIDGNLVKFFQGHNLFGSCDIVGLVCDFMDHIC
                     SMESLGLHPTDFQKQAWRRGLFKLSRVDVTSMYRLNNRNDVLSWISSAEYSSTMAHRGR
                     GQLTKGSTLYFGKHSRRWSLKFYSKGQEVNCKGHRLPLELPSRDKLLAYAEPALRSELT
                     LRSMTLKETDLQLAANWPRNAEGITRIFEKYLAGLNMSDTRTIPAEQLAELPSGARLAY
                     QSWLEGHDLKNMLPHNTFYRHRRALLTLGVDIATVLPREKSNVVPLVRVLEAIPMGIPD
                     WAYGTPLYHQPVGF"
                     /name="phage/plasmid replication protein, II/X family"
ORIGIN
        1 tgaggttctg aactttggta tggcattccg tatctcgcgt tgatatgttt gtcgttaact
       61 ggtataaatt tacacagtta accaaaaacc taggatgttt tatgaaaacc aaatcagcag
      121 gtaacaaatt ggctacgtat ctccagagtc actcaattat tcgcgacagt tcagagggcg
      181 tcagcggtaa aaatatttcg ttttccgttt cgatgcctat tgagcggtat taccttttca
      241 aagaaatatc aaatcgttac aacatctcta tgtctcaaat tgttaacgta gctttgcatg
      301 ctttcgattc tgaattacta ctttcccttg atagagagtc atcttttgaa attgcccgcg
      361 ctgcggatga tgggattacg tcagatattg gcggtgagcc agtttcttac tggtattcaa
      421 aggttcttca gaaagcagcg caggaagatt ttgcgcgttt ctgcgaggcg aatcctgaca
      481 ttccagaaga gcaccttgat gatgcctatc agtcttatct tgcgcagtct gccagagagg
      541 agattttttg atgctttcta tgaccggaca gcttttgaac gtcttcgaaa atccccgcgg
      601 cgtgactaaa gagggtcgtg agtatggtgg gaattataaa attcaggttc tgggggctgt
      661 caccctccag aacggtgaaa caaaaaatga cctcgttgac ttaacctgtc acgatattgc
      721 ggcgttcagg ccgcttttgg ggcagcgaat ttctttcccg attggggtta tgtcgatggg
      781 taagaatcag actgttttct tcattcccaa gggtgggatg ccggagccgg ttgggggttg
      841 agtgcagtat agtgatactg cacttttgta ccaaaatggt acggaagact aaaactatga
      901 tgatagattg gctgacgtta cgtatcccct gtgaccactc cagagagatt gcaggcgggg
      961 cgatggtgtc ttacaaggat gcttgggatt tcgatgctgg cgcggattgg tgcttcacaa
     1021 aaccgcttgc ggttgtgggg tcacatgatg ccaatgtcag gttaaaaaca tctggtttca
     1081 gcaacgaagg ccagtccggt actgagcttc atatcgacgg aaatctggtg aagttctttc
     1141 aggggcataa cctgtttggc agttgtgaca ttgttggtct cgtttgtgac ttcatggatc
     1201 acatctgcag catggaatcg cttggcctgc atcctactga ttttcagaaa caggcatggc
     1261 gtcgggggct gtttaagcta tcccgtgttg atgtgacgtc catgtatcgg ctcaataacc
     1321 gcaacgatgt actgagctgg atttcatccg ctgaatattc ctcgacgatg gcgcaccgtg
     1381 ggcgtggtca gcttaccaaa gggtcaacgc tttattttgg taagcattcc cgccgttggt
     1441 ctctcaagtt ctattccaag ggacaggaag ttaactgtaa ggggcatcgt ttgccgcttg
     1501 aactaccttc ccgtgataaa cttctcgcgt atgcagagcc tgcattaaga tctgaattaa
     1561 cgctgcgttc aatgacattg aaagaaaccg atctccagtt ggcagcgaac tggccgcgaa
     1621 atgcggaggg tataacccgt atatttgaaa aatatctggc agggctaaac atgagcgata
     1681 ctcgcaccat tccggcagaa cagcttgccg agttgccttc tggcgctcgc ttggcttatc
     1741 agtcatggct cgagggtcat gatttgaaaa acatgcttcc tcacaatacc ttctatcgtc
     1801 atcgtcgcgc cctgcttact ttgggtgttg atattgctac ggtgcttcca cgtgaaaaat
     1861 cgaacgtggt tcctcttgtt cgcgttctgg aggctatccc gatgggtatt cctgattggg
     1921 cgtatggtac gccgttgtat caccagcctg ttggcttctg acgcgctccg cttgtcgaat
     1981 ccccgtttta acctgtacca gtccaggcta aaacggggat ttttttgcct tcgttacacc
     2041 ggaacttaat gactcttttc agcaagcggc tacggcatgt gtatgccccg tagctctgcg
     2101 aggagtatac acatgccgtg ggtgcttgcc tgatgtggga tattgtgatc agatgggcgc
     2161 agagacagga attatccgtt cctgcctctg ctatcacgaa aacctttcag ggaggtctcc
     2221 attactgacg ctaatcctac catgcttgat ttgcttatat ctcaccgtag cgctgttgac
     2281 gcctttaacg ccttttcttc tatccatcct gttgatgatg accttcatcc cctcatagag
     2341 cttttgagcg acaatttgcg cacatctttc cttgctatat atccgcaact ctgtggcctg
     2401 attccgtcaa atgctgaatc gcggggcccc gctggtgagc cagtgggttc cgattcagtg
     2461 attgacggca ctccgtaaga aaaaaaactg gcaatgggtg atggttcacg tagtgaggcc
     2521 atcaccctgc aaagcagcga actttactgt ggttatccgg ggtgaggttc tgaactttgg
     2581 tatggcattc cgtatctcgc gttgatatgt ttgtcgttaa ctggtataaa tttacacagt
     2641 taaccaaaaa cctaggatgt tttatgaaaa ccaaatcagc aggtaaggg
//
