LOCUS       NZ_AP022461             2883 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STW0522-62 plasmid pSTW0522-62-9, complete
            sequence.
ACCESSION   NZ_AP022461
VERSION     NZ_AP022461.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195902
            Sequence Read Archive: DRR199707
            Assembly: GCF_015136055.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STW0522-62
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 550x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,030
            Genes (coding)                    :: 4,914
            CDSs (with protein)               :: 4,914
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 34 of 116
            Pseudo Genes (incomplete)         :: 80 of 116
            Pseudo Genes (internal stop)      :: 22 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            Pseudo Genes (short protein)      :: 1 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022461.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STW0522-62"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTW0522-62-9"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            102..410
                     /locus_tag="JLF39_RS25540"
                     /old_locus_tag="STW0522ENT62_P90010"
     CDS             102..410
                     /locus_tag="JLF39_RS25540"
                     /old_locus_tag="STW0522ENT62_P90010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045351245.1"
                     /translation="MTDKLKGTASVLNQTKTYEELVQKHSPEVANGLLANAINNALPNA
                     GITSNDVAGFSKVTTALRTGEVDLAKTAEEANADAEAVSANILAGLTAKQKSTDEIK"
                     /name="hypothetical protein"
     gene            complement(514..1149)
                     /locus_tag="JLF39_RS25545"
                     /old_locus_tag="STW0522ENT62_P90020"
     CDS             complement(514..1149)
                     /locus_tag="JLF39_RS25545"
                     /old_locus_tag="STW0522ENT62_P90020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045351244.1"
                     /translation="MNDIFSLIESSRKIKKESIASTVRMPESLHTFIETLACDLELSKQ
                     EIMLKLLEQGARSAQEALAEIEKKELVELGAVEQLEPQTAAGFHILNTNKAHSDEDSEW
                     MLGKGIAAAFYDPWKWNINRIKPNEVVFLYENGKGIVAYGRGTGEVKIRDYHGDKDECH
                     YQELEGFKILENPLSAAQIKKILERNVVFLRTMSAMPDGQKVLNLIEG"
                     /name="hypothetical protein"
     gene            complement(1486..1722)
                     /locus_tag="JLF39_RS25550"
                     /old_locus_tag="STW0522ENT62_P90040"
     CDS             complement(1486..1722)
                     /locus_tag="JLF39_RS25550"
                     /old_locus_tag="STW0522ENT62_P90040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016230891.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            2561..2674
                     /locus_tag="JLF39_RS25555"
     CDS             2561..2674
                     /locus_tag="JLF39_RS25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058651821.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPLAVATGN"
                     /name="hypothetical protein"
     Typing          2181..2670
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.112"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 ggctgtttag cagtgatttg gcttcagtat gagtgcccgc ctaacgcgag tcttgtacat
       61 aaaattttct actcctgact catcacagac tagaggttta catgacagac aaactcaaag
      121 gaacagctag cgttcttaat caaaccaaaa cttatgagga attggttcag aagcattcac
      181 ctgaagtagc taacggcttg ctggcaaatg ctatcaataa tgctttgcct aacgcaggaa
      241 taacttcgaa tgacgtcgct ggattcagca aagtgacgac ggccttgcgt acaggagaag
      301 ttgatctagc aaaaacagcc gaagaagcca atgccgatgc ggaagccgtt tcagcaaata
      361 ttcttgctgg cctgacagca aaacaaaaaa gtacagatga aataaaataa tttcccgcag
      421 gtacaaaaaa gcccgaacta gacgggccaa ggtttcgtat gtaattactt ataaaaaaat
      481 tttaaccgca ctcaacccca tcaagtgctc ggcttaacct tcgataaggt tcagcacctt
      541 ttgaccatct ggcatagccg acattgttct caaaaacaca acattccgct ccaatatctt
      601 cttaatttga gcagccgata gcggattttc aagaatttta aacccttcca gctcttggta
      661 gtggcattcg tctttatcgc catgataatc gcgaatttta acctcaccag tacccctacc
      721 ataggcaact atgcctttac cgttttcata cagaaacact acttcattgg gcttgattcg
      781 attgatgttc catttccatg gatcataaaa agcagcagcg attcctttgc caagcatcca
      841 ttcagagtct tcatcgctat gagctttgtt tgtattcaaa atatggaagc cagcggctgt
      901 ctgcggctcg agttgttcta cagcgcctaa ctcaaccaac tccttctttt caatctcagc
      961 taacgcctct tgagcactcc tggctccttg ttcaaggagt ttaagcatga tctcttgctt
     1021 tgaaagttcg agatcgcagg ccagcgtttc gataaacgta tgcaacgact cgggcattcg
     1081 cactgtggaa gctatcgatt cttttttaat ttttctggag ctttcgatta aagaaaaaat
     1141 gtcattcatt ttatgaacct taaattgggt tggttataga agcatatagc gaatcaaaaa
     1201 acgatgcaat aattgaatca aaatagattc taactccagg tgatagatga tggcgtatgc
     1261 acgaatagtc attacgatgt gtatttacca taacatccgt tatcagcacc ccctgacagg
     1321 ccctcagcgc aattctggcg catcagagcc gtttccggca catttcctca cccgcatccc
     1381 ggaaaaacgc ccctgatgat ttctgcgcga ctgcatggct atgcacgcgg gtgaatcgca
     1441 ggtgatttcg cagcgtcagc accactaaac cgccaaaatc cgcaatcagc ggcgcggcgc
     1501 gcgcggacgt gaccaggcaa aatcggactg ctgccgctca tcctcacgta atgcacgctg
     1561 gcggtcggcg taacgctgcg actcaatctg cgcacgcgct ctgttcagga cgatgtgccg
     1621 gttaggctga ttgagcttct tcccggccgc acgttcggca gcggctatag cgtcgtcgat
     1681 aaactttttc tcagcggggg tcaggtttgt cagtctcgtc atgtttatct ccgtatgaga
     1741 atagtccctg cagtctgcca gacacagtag gaacgatgaa gcgtaagcga cggtgaaagg
     1801 cagtcagaaa cggtgaacgg gagcggattt taatccggtg ccgtgaggcg ttggacaagc
     1861 cgcaggcgcg tcagtggatt tagcgggttt cggggcgcag ccctgaacca gtcacgtagc
     1921 gctagcggag tgtatactgg ctaaacttca gagatgccaa ggaagggaca ggatatgttt
     1981 tgtagcaccg ccaccacggt ggcgatactc caaaacgcct ggtcagggca cagccccgac
     2041 accccagagc cgactgtagg tcactgggcg aacgcggcgg aatgatgtcg gatgacgtca
     2101 ttttgcggcg tttgccctat cctgcatcgt attaaaaaat gcagctatgg cgcgtacggt
     2161 gccaaagata agcccggaga tattccgctt cctcgctcac tgactcgctc cgctcggtcg
     2221 ttcggctgcg gcgagcggta ccggcttact ggcggggcgg aaatttcctg gcatcttcca
     2281 ggaagaagct taacagggaa gcaagaaggc cacggcaaag ccgtttttcc ataggctccg
     2341 cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg
     2401 acttaaagat accaggcgtt tccccctggt ggctccctcg tgcgctctcc tgttcctgcc
     2461 tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tatctcattc cacgcctgac
     2521 actcagttcc gggaaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
     2581 gtccgactac cacgcccgtt ccggtaacta tcaacttgag tccaacccgg aaagacacga
     2641 caaatcgcca ttggcggtag ccactggtaa ctgatttaga ggaggagagt cttgaagttc
     2701 ctggtgcgat gcagctacac tgaaaggaca gctttggtga ctgcgctcct ccaagccagt
     2761 taccttcgga agaaagagtt ggtagctctt gattccggca aacgaaccac cgttggtagc
     2821 ggtggttttt ttgtttacag ggaatgagat tacgacgaga gtaaaaggat cacaagaaga
     2881 tcc
//
