LOCUS       NZ_AP022460             3291 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STW0522-62 plasmid pSTW0522-62-8, complete
            sequence.
ACCESSION   NZ_AP022460
VERSION     NZ_AP022460.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195902
            Sequence Read Archive: DRR199707
            Assembly: GCF_015136055.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STW0522-62
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 550x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,030
            Genes (coding)                    :: 4,914
            CDSs (with protein)               :: 4,914
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 34 of 116
            Pseudo Genes (incomplete)         :: 80 of 116
            Pseudo Genes (internal stop)      :: 22 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            Pseudo Genes (short protein)      :: 1 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022460.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STW0522-62"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTW0522-62-8"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            102..359
                     /locus_tag="JLF39_RS25520"
                     /old_locus_tag="STW0522ENT62_P80010"
     CDS             102..359
                     /locus_tag="JLF39_RS25520"
                     /old_locus_tag="STW0522ENT62_P80010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_097734894.1"
                     /translation="MEQAAFLFSGVGGMLLTEMEISNIRNITLIARRLAWINAKRTHID
                     IPSFAELLFSGQKALFFEQYNYFHSRRYTTAGTSKPPDLP"
                     /name="hypothetical protein"
     gene            complement(625..1155)
                     /locus_tag="JLF39_RS25525"
                     /old_locus_tag="STW0522ENT62_P80020"
     CDS             complement(625..1155)
                     /locus_tag="JLF39_RS25525"
                     /old_locus_tag="STW0522ENT62_P80020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     gene            complement(1158..2093)
                     /locus_tag="JLF39_RS25530"
                     /old_locus_tag="STW0522ENT62_P80030"
     CDS             complement(1158..2093)
                     /locus_tag="JLF39_RS25530"
                     /old_locus_tag="STW0522ENT62_P80030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_045282372.1"
                     /translation="MATYHLSVKFGGKGKALAHASYITREDKFSQRQDLEHTEHGNMPE
                     WARDEPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLALVRDFVKQETGDKHSWT
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKTQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            2260..2646
                     /locus_tag="JLF39_RS25535"
                     /old_locus_tag="STW0522ENT62_P80040"
     CDS             2260..2646
                     /locus_tag="JLF39_RS25535"
                     /old_locus_tag="STW0522ENT62_P80040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045282373.1"
                     /translation="MRISNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDAAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     Typing          complement(1162..2093)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.177"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctcacaaaac gtggattatc tggttagtga ttcgcagcaa caaagcgtcc cggttcaaaa
       61 cgaatcgtaa gacgtagttt cccttgatgt agggcttgcc gatggaacag gcggcttttt
      121 tattctccgg cgtggggggg atgctgttga cagaaatgga aataagtaac atccggaata
      181 ttacgcttat tgcccggcgt cttgcctgga ttaatgccaa aagaactcat atcgatatac
      241 catcgtttgc agaactgctt ttctccggcc aaaaagcgct gttcttcgaa caatataatt
      301 attttcactc acgacggtac acaacggcag gcacgtcaaa gccaccggat ttaccataac
      361 atctcttgcc cgcaccgctg aaacgcgctc agggcggttt tacgtcctga gccataaaaa
      421 cccccggctt ctcaccgaaa accgctcaga atcgcatacc gtgattttca gccccatgca
      481 taaccatgca cctgactgac tggcgagaac ttccggcctg aattcaggcc ggaagtctca
      541 aaagacgccc caaactcggc tgaaaacggt gaacgggtgc gcaatttatt gtgcgaacgt
      601 gaggcgttgg acaagcgcag cgcgtcagtt ctttttcttt tccggtttct gttccgttgt
      661 cagcagcttt tcaaggtctg ccactttctg atagacggca gcagacgcgt tgtaattgtc
      721 ctgtagttcc gttttcacaa accagcttgt ggcaaatatc agtacgacaa aggcaaccgc
      781 aaccccagca aaccacttcc agctgtaacc ccgcagcgtg ttgtactccg cgatgatgtt
      841 actgcggata atggaaattt ccttgtccag cgcgtccgca ttgattttca tgttcccgac
      901 ggatttttcc agacgctgaa aatcagcgct gatacgctcg ccaatcagcc ccgcaacatc
      961 cattttctgc acttcctgcc ggacttcatc ccttacctgc tgtgccacac tgtccgatat
     1021 ttcccggtag cctttcaggg tttcccttgc caccgccagc gaatccgcca cggctttctg
     1081 ctgctctttt gcggcctctt tcagatgctc cgccgacagc aggagtgtga gaagtttttc
     1141 ttcggtgctc gccatagtta cctgcttatg gaaaatccgc gtgacaggga tttcacgcgc
     1201 tctatttcaa catttccctg ttttgtgacc cgtgcagcca tctcccgccc gtactgctgc
     1261 gagatatcat gcgtcagaga tctgaccagt tccttccggt ttacctcgat accgctggca
     1321 ttcatccagc tacggtcgct gtggtcgata tgtttcgcct gttcaggcac atccacgact
     1381 gaaaacgtcc ggctgtcttt aatatcctgc ctctggctgt cgtccagtcg tccggctgct
     1441 tcaaaaccga gatgctgttc cggtttccgg tcgataccct gtgttttcag tgaccggcta
     1501 tcaataaaac cctgctcaaa gccatatcgt ttcatatgcg cgttgtgcgt acgctcccag
     1561 cgctcccgca gggctttcag tgcctgtttt tgttctgtag gggttaaatt atgccctgag
     1621 tccttgcgcg cgccgccacg cgccggaaat ttggcgttat agcgcctgaa atactgttcg
     1681 ggactgcgct caataccgtc attgacgcgc tggctcatca tgatatgggc gtgcggctgt
     1741 tcgccgccgt caatgctggc tttcggattg tggatcgcaa acgtccatga atgtttgtcg
     1801 cccgtttcct gcttcacaaa atcccggaca agcgccagcc gctgcgcttc attcagctcg
     1861 cggggtagag cgatctcaat ttccctgtac gttgaaccgt tggcacgttc gaacgcatcc
     1921 gcagcctgcc agaaatgggc gggttcatct ctcgcccatt ccggcatatt gccgtgttcg
     1981 gtgtgctcga gatcctggcg ctggctgaac ttatcttccc gcgtaatgta actggcatga
     2041 gcaagggctt tacccttgcc gccaaatttc acgctgagat gatacgttgc catgttgtgt
     2101 cagcccccgg cagggcgcac tgtttctgcc cgcagggaga aacatataag tgcgctttac
     2161 tctaatttaa agaattatga gtaaagataa gtcatgcgac ttctgaccgc aagttatata
     2221 ctttccccgt cggaaatatc gggatgggga agttaaacga tgcggatcag taacgtcgaa
     2281 tggcttaaaa agcgcatagc cttcatcaga aagctgggag aaaaaacggc acggcaacgg
     2341 cagattatcg atctgctgga tgatgaagct tcgctcagtg agactgaaag acgactgctc
     2401 cacgttctgg caaccgctga aaaaaacgcg ttgcaggaac gggatgccgc acagaaaaag
     2461 gcggttcagg cgcggatttc agggcagaaa aaccgcaggg aacgcaatca cagactgttt
     2521 ctggcggctg gtctgatgat tgacgcggga ctggttgata caaaaacggg tgagttgaat
     2581 tttgaccacg aaaaattact gactgcgctg aaacgggtac gctacgatct ggaaacatcg
     2641 gcgtgaacgt gcattctgca aggccggaga aatgccgctt cctcgctcac tgactcgcgt
     2701 cgctcggtcg ttcggctgcg gcgagcggta gggattttcg gataggagca tgaattttct
     2761 tgctccagat gtacaaaagc cccgtgacag aatcacgggg cttgagtact ctaattcttg
     2821 cagatacctg ccaggatcag acgtcacaat caaatcctag cagggctggc taggaatgtc
     2881 actaccagct taataccatt caacgacatt aaacccatat ctgcaccttg actctcaggt
     2941 ctacgccccg gactccggca ggataaacag gcaagcggtg ggaacctgac cagctcggga
     3001 aagaagctgg ctctacccgg tctcacgtta accaataacg tacctgataa agtgagagtg
     3061 aaggcagcgg ttgcggtgaa gcgtgattgc caaggtctgg cgcaacaccc acaaaatcga
     3121 agcagcatac gaacggcctg atgctggtcg cttcgctccc cgttttctgg tgtacccacc
     3181 acgggggcat taggctttct ttgccctaat atgggtttgc gactctcaaa tacaggttgc
     3241 cctaatcact taacgctatt attgttctat tgaagcctta acctaaaccc a
//
