LOCUS       NZ_AP022459             3631 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STW0522-62 plasmid pSTW0522-62-7, complete
            sequence.
ACCESSION   NZ_AP022459
VERSION     NZ_AP022459.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195902
            Sequence Read Archive: DRR199707
            Assembly: GCF_015136055.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STW0522-62
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 550x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,030
            Genes (coding)                    :: 4,914
            CDSs (with protein)               :: 4,914
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 34 of 116
            Pseudo Genes (incomplete)         :: 80 of 116
            Pseudo Genes (internal stop)      :: 22 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            Pseudo Genes (short protein)      :: 1 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022459.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STW0522-62"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTW0522-62-7"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            102..560
                     /gene="traD"
                     /locus_tag="JLF39_RS25505"
                     /old_locus_tag="STW0522ENT62_P70010"
     CDS             102..560
                     /gene="traD"
                     /locus_tag="JLF39_RS25505"
                     /old_locus_tag="STW0522ENT62_P70010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_052862006.1"
                     /translation="MHENEWLEKRLAYIRGLNAPNEQQRLLLMLAQLSQPTPNDRRKLG
                     VLIRAEKAAERALKARADAAKVLNAERRAVRKARDHELYKAAGLMGVAGVVDRETGRLN
                     VDAGLLVGLLMELAAGTLPENLHQEALKSRGDAAIAAREKKNKTNQAG"
                     /name="traD"
     gene            1239..1940
                     /locus_tag="JLF39_RS25510"
                     /old_locus_tag="STW0522ENT62_P70020"
     CDS             1239..1940
                     /locus_tag="JLF39_RS25510"
                     /old_locus_tag="STW0522ENT62_P70020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_061371533.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDAPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEAKLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     LKGEAKVEITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     ISLDDFRSILELEDKYSDFKILNRDLIKPAIAELNEKSDLAVTVETVKKGRTVVALRFR
                     FKEDKQIKMAV"
                     /name="replication initiation protein"
     gene            complement(2121..3560)
                     /locus_tag="JLF39_RS25910"
                     /old_locus_tag="STW0522ENT62_P70040"
     CDS             complement(2121..3560)
                     /locus_tag="JLF39_RS25910"
                     /old_locus_tag="STW0522ENT62_P70040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_052862116.1"
                     /translation="MAIARLSMKAGMKGRGADHSAYISREGKYANRLERGERLEATDHG
                     NMPEWAKDNPQQFWLAADAYERKNGTSYREMEIALPRELDSEQREALVRDWTEQELGDK
                     HAYQWAIHVPTAADGGEQPHVHLMFSERQRDGIERDPEQYFKRYNSRNPEAGGARKGYG
                     EHGGQPRTLEQRREELVALRGRWEAKANEHLERAGQRERIDMRSYTAQGKDAIPEKKQL
                     PSEWRDERKRAEILDFRAAKAEQEQAQKDAGKVLYEHNSAVEHYRGVVAAVPEHRLKNS
                     SPDSIALDLREKLVGQRVDSDPAVLAAREQEEKTGKALENARQERDKTRQEAEQQRQEQ
                     QRWEEENRMRRWLHDKGIKKDERYTGLQQQREKSEQQAQAAVQKEQQAEMQHRQAVTAR
                     EETETDVRQQANEKWFEHHHTAVKEAAAERQRELERQEREQQQAKQAEYQQLIAQQQRQ
                     REQEKEQEHTPERQHGRGMSM"
                     /name="MobA/MobL family protein"
     Typing          1240..1940
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.863"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2125..3560)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.747"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctaagcgcgc cttccgaaaa tcgaagattt ccgagaagga tacaccctcc gccgcaactg
       61 tggtagtttt atgcaggcac gaaacgaaca gcgggggaaa aatgcacgaa aatgaatggc
      121 tggagaaacg gctcgcatat attcgcggac tgaacgcacc gaacgaacag cagcgccttt
      181 tgctgatgct ggcgcagttg tcccagccaa caccgaatga ccgccgcaag ctgggagtgc
      241 tcattcgtgc tgaaaaagcc gcagagaggg cgctaaaggc tcgcgctgat gccgctaaag
      301 tgctgaatgc cgaacgtcgc gccgtccgca aagcgcgtga ccacgaactc tacaaggcgg
      361 ctggtctgat gggcgtggcc ggcgtggttg accgtgaaac cgggcggctc aatgtggatg
      421 ccggcctgct tgtcggattg ctcatggagc tggctgccgg tacactgccg gaaaacctgc
      481 accaggaagc acttaaatct cgcggtgatg ctgctatcgc tgcccgtgag aaaaaaaata
      541 aaacgaatca ggctggctga tgaacctcac acttgtttgc caatagccct gagaggccgc
      601 agaaatcatc ctgcggcttt tttttgcctt atggtaggga aacgtacagg gaaaagaaaa
      661 aaacgctcac agcgccttac agagcgtttt agaactatca gccgatccgt tgctggcttc
      721 gggctgtgac gactgccgcc cctccatcct gaaaagaccg ggtttcccct tcaccggcaa
      781 tgaagcgaca ccagagcctg acggtcgaag acgtacagcc tttcagcgat ttgcccgccc
      841 gtccgggcgg gtgctacctc ctgccatagc gcgttattca cagggattta cggccattca
      901 gcgtcctgca gaacaggcat atttataacc tactgtttat tttgatgttt ttttttcgtg
      961 tcgtcgtttt atccacagcg ccgccgttaa gtttttaaga tctttttttt agatcttctt
     1021 ttttaaaacc tttttcggtc ttgtaacccc atgattttaa taatgttctg gcaagatagg
     1081 tgaggaaatc gggggttata ggtgaggaaa tcgggggtta taggtgagga aatcgggggt
     1141 tatatccaca aaaaaaggtg agtaaaaaaa aacattgatc accttttgat tgggtgataa
     1201 aacaaaataa acactcacca taagaacata aattactcat gtctgatttg atagcctaca
     1261 aatccaacgc ccttatcgag gcaagttaca agctcacctt gcaggaacag cgcttcctgt
     1321 tggtctgtat cagccgcctg aaatccgggg aagatgcccc ttcgccggat gaacaaaaaa
     1381 ccatgaccat cacggccgca gagtttcatg atgcttttcc tgatatggga cggcagcacg
     1441 ctgaagccaa gctcaaggaa gcgatagatc gcctctggga caggtcagtc atcatcaaaa
     1501 acgaggagaa gcgcgaggag ttccgctggg tgcagtaccg ggcgcagtat ctcaaaggtg
     1561 aggcgaaagt agagataacc ttctccgatg ccatcatgcc ctatctgaca cagctcaagg
     1621 ggcagtttac gcgggtagtg gtgaagaacg tatccagcct ctccagcacc tacagcatcc
     1681 gcatttacga gctgctccag cagttccgca gcaccggtga cagaactatt tcactcgatg
     1741 acttccgatc catactggaa ctggaagata aatattctga ttttaagata ttgaacagag
     1801 acttaataaa accggccatc gctgagctga acgaaaaaag cgatctagcc gtcaccgtcg
     1861 agacggtgaa aaaggggcga accgttgtcg cgttgcggtt ccggttcaaa gaggacaagc
     1921 agatcaagat ggccgtatga ataacgaaac caaaaagagc ctaagccgtg gatctcaccg
     1981 caggaatgca acatgctgac ggcgacggag ggcgtggccg ttgtggttct caaccgcgcc
     2041 ggcgctcccc gccgcgatgc gatagagctg gttcccgaat accgcgccgg ctgaaaaaaa
     2101 aggcaccgca aggtgccttt ttacatgctc attccgcgcc cgtgctgccg ttccggtgtg
     2161 tgttcctgct ctttttcctg ctcacgttgc cgctgttgct gggcgatcaa ctgctgatat
     2221 tcagcctgtt ttgcctgctg ctgctcccgc tcctgccgct caagttcccg ctggcgttcg
     2281 gcggcagcct ctttcacggc cgtatgatga tgctcgaacc atttttcatt ggcctgctga
     2341 cggacatcag tttcggtttc ttcccgcgca gtgacggcct gccggtgctg catttctgcc
     2401 tgttgctcct tctgcacggc ggcctgtgcc tgctgctctg atttttcccg ttgctgttgc
     2461 agtccggtat aacgctcgtc ctttttgatg cccttgtcat ggagccagcg gcgcatccgg
     2521 ttttcttctt cccatcgttg ctgctcctgc cgctgctgct cggcttcctg ccgggttttg
     2581 tcccgttcct gccgcgcatt ctccagcgcc ttaccggttt tttcttcctg ctcccgtgcg
     2641 gccagtacgg ccggatcgct gtcaacgcgc tgcccgacga gtttttctcg caaatccaga
     2701 gcgatgctgt ccggtgagga gtttttcagc cggtgctccg gcaccgcggc cacaacgccg
     2761 cgatagtgct ctaccgcgct gttgtgctca taaagcacct tgccggcgtc tttctgtgcc
     2821 tgttcctgct cggcttttgc agcgcggaaa tccagtattt ctgcacgctt ccgctcgtcg
     2881 cgccattcac tcggtagctg ctttttttca ggaatggcgt ccttgccctg cgctgtatag
     2941 ctgcgcatgt cgatacgttc gcgctggccg gctcgctcaa gatgttcgtt agctttggcc
     3001 tcccaacggc cgcgcagggc gacaagttcc tcgcgccgct gctccagcgt gcgcggctgg
     3061 ccgccatgct caccgtaccc cttccgcgcc ccgcctgctt ccgggttccg gctgttatag
     3121 cgtttgaaat actgctctgg atcgcgctca atcccgtccc gctgccgttc ggagaacatc
     3181 aggtgtacgt gtggctgctc acctccgtca gcggcggtgg gaacgtggat agcccactga
     3241 taagcgtgtt tgtcgcccag ctcctgctcc gtccagtccc tgacaagcgc ctcacgctgt
     3301 tcgctgtcca gctcacgagg cagggcgatt tccatctcac ggtatgaggt gccgttcttg
     3361 cgctcatacg catcggcggc cagccagaac tgttgcggat tgtccttcgc ccactccggc
     3421 atattgccgt ggtcggtggc ctccagacgc tctccccgtt ccagacgatt tgcatacttg
     3481 ccctcacggg aaatgtaggc cgagtggtct gcgccacggc ctttcattcc cgctttcatg
     3541 ctgagtcgtg caatcgccat ttctttaccc tgtaaatttg gggtgtgggg cttgccacac
     3601 cgcgcagttt ctcaccgaag gggagaaact a
//
