LOCUS       NZ_AP022458             3862 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STW0522-62 plasmid pSTW0522-62-6, complete
            sequence.
ACCESSION   NZ_AP022458
VERSION     NZ_AP022458.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195902
            Sequence Read Archive: DRR199707
            Assembly: GCF_015136055.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STW0522-62
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 550x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,030
            Genes (coding)                    :: 4,914
            CDSs (with protein)               :: 4,914
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 34 of 116
            Pseudo Genes (incomplete)         :: 80 of 116
            Pseudo Genes (internal stop)      :: 22 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            Pseudo Genes (short protein)      :: 1 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022458.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STW0522-62"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTW0522-62-6"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            join(3629..3862,1..111)
                     /gene="mobC"
                     /locus_tag="JLF39_RS25490"
     CDS             join(3629..3862,1..111)
                     /gene="mobC"
                     /locus_tag="JLF39_RS25490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_032415722.1"
                     /translation="MSYRERYQRKNFVSLCLNDEELSELENIAERLNMKRAAAAREILF
                     TNSKRLKSQIKKNDNSEILFLYSKISNNINQIAKKMNTNLDKFLSGNGEEFALLIEEIF
                     EDMEKLKNDT"
                     /name="mobC"
     gene            101..1525
                     /locus_tag="JLF39_RS25495"
     CDS             101..1525
                     /locus_tag="JLF39_RS25495"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200925425.1"
                     /translation="MIHKHIGGKKNQRSVKNSLDYLLRTNKPEEQKFVEVLSQTNRSDI
                     ENFNEYIKEKNFKNPFITGVLSFEEKDIDPELKTKIMNDFEDMMFSGIDPENRPPVIWI
                     QHKDKNRLELNYTTFNSLMNKKHFQCYYHLSDKKLFNSFCEKINYENNISSVLDVKEHK
                     EKNTLVNTLNNKIPADKKAIVEKLQEDILSKIITEEINNREELISFIQSKNIIINRVRE
                     NAISIKFSKEDKPISLKGDIYEQDRDYKTYTTESTIDHRADQQYVENQLRHHRENFDTE
                     LKKRHSRNRTRFKSTQSKDQKNVEFRIKKANDSKNVRSEIPDNNNVSFRNEPDIDFFHN
                     NKTIEDVSNEQNFTNDKTKPREEEPAKFDLSREIETANTQQQFIRSRVEEVRRNQQQFR
                     KSLDRTQERSGVCRRSLHSIIHNIRRYVRTFSIHTRQNERRQKIEHRQKIFLEKLKTQQ
                     EKNNQRSVVRSRFKPY"
                     /name="hypothetical protein"
     gene            1955..3358
                     /locus_tag="JLF39_RS25500"
                     /old_locus_tag="STW0522ENT62_P60030"
     CDS             1955..3358
                     /locus_tag="JLF39_RS25500"
                     /old_locus_tag="STW0522ENT62_P60030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200925424.1"
                     /translation="MNEKSIEEILAERQLKEFNESKFGQFFNNQKLKEQELTYLNNKAL
                     DIVNEEDLFEIMSLIPQPFITEENKNVDVLSLIYNNFLENTEIPLTLPIFSFLSFLSAF
                     CVKNEITYTIPLSDEKSPLDTWITILAPSGSAKTFSNSQIKKVIPVGIDGKKVIESNFV
                     KPNGPAKFIEELSNLPETKNGENQYGFWIEDEVSQMFKQVEQIGSPLSEIKEYLLKSYD
                     HEKLTRKSKKETIETKNIVLTLLFINTFESYANNISNESMTDGLMRRFNLIYTEKDSER
                     DFTDYSLYNLKKIRDETLQDKISELFNSIKPDQNFTFSDNCFKLYQKYFKIFWHRKYKT
                     ILPEKENFYRTYMMQSWKFAVFYHLILKREGTEIQGDCLVFGMKISLLFANSMKKFFDF
                     KLNKKLTPKKKEKIKIDFTKYIEYLNENTDITLRTFCRKFNLKKADSISILKSLECNNY
                     QHKLIEELE"
                     /name="hypothetical protein"
ORIGIN
        1 aaaaaaatga atacaaatct tgataagttt ttgtcaggga acggagaaga atttgcatta
       61 ttaattgaag aaatattcga agatatggag aaattaaaaa atgatacata aacatatcgg
      121 aggaaagaaa aatcagcgaa gtgttaaaaa ctcacttgat tatttattaa gaaccaacaa
      181 accagaagaa caaaaatttg ttgaggtttt atctcaaaca aacagatctg atattgaaaa
      241 tttcaacgaa tacataaaag aaaaaaactt caaaaaccct ttcataactg gtgttttatc
      301 ttttgaagaa aaagacatcg atccagaact aaaaacaaaa ataatgaatg attttgaaga
      361 tatgatgttt tcaggaatag atccagaaaa tagacctccg gtcatttgga tacaacacaa
      421 agataaaaac aggcttgaat taaactatac gacttttaat tccttaatga ataaaaaaca
      481 ttttcaatgt tattatcatt tatctgataa aaaattattc aactcatttt gtgaaaaaat
      541 aaattatgaa aataatatca gttctgtttt agatgttaaa gaacacaaag aaaaaaacac
      601 gcttgtaaat actttaaata ataaaatacc agcagataaa aaagccattg ttgaaaaatt
      661 acaggaagat attttatcga aaataatcac agaagaaata aacaacagag aagaattaat
      721 atcatttatt caatctaaaa acataataat aaatagagtt agagaaaatg ctatatcaat
      781 aaagttttca aaagaagata aaccaatatc actgaaagga gatatatatg aacaggacag
      841 agattacaaa acttatacaa cagaatcaac aatcgatcac agagcagatc agcagtatgt
      901 cgaaaaccaa ctcagacacc atagagaaaa ttttgacaca gagcttaaaa aaagacacag
      961 cagaaataga actcgtttta agtcaacaca gagcaaagac cagaaaaatg ttgagttcag
     1021 aattaaaaaa gcaaatgaca gtaaaaatgt tagatctgaa ataccagaca ataataatgt
     1081 ttcttttagg aatgaacctg atattgattt ttttcataat aataaaacta tcgaggatgt
     1141 aagcaatgag caaaatttca caaatgataa aacaaaacca agagaagaag aaccagcaaa
     1201 atttgatcta tcaagagaaa ttgaaacagc taatacacaa caacaattca ttcgttcaag
     1261 agttgaagag gttagaagaa atcaacagca attcagaaag tcgcttgata gaactcagga
     1321 acgatctgga gtatgtagaa gatctctaca ttcaattatt cacaatatta gaagatacgt
     1381 cagaacgttt tcaatccata caagacagaa tgagagaaga caaaaaatcg aacacagaca
     1441 aaaaatcttc cttgaaaagc tcaaaaccca gcaagaaaaa aacaatcaaa gaagtgtcgt
     1501 cagaagtcga tttaaaccgt attagaaccg ttctgaacac tcttctaacc cactacgaca
     1561 aaaccttaac cgtgaacaag tgagaaccag ccagaggctg gtttttgctt gtgaggggtt
     1621 ggcctgcccg gcgagggcgg tttttctgtc ataactttgt cggagttttt gacaagaacc
     1681 tgtctgaata tttttcattt tcttatgaca taaaaaacag ctttttttct caactatgat
     1741 tttaagacaa gtgtcataac ttttgtcata ggttttgtca taacccaaaa aagcagtaaa
     1801 atccttatgt atcaaggctt tatctgttgt gtcatagtgt catagtgtca taaatcaagg
     1861 tttaaaacgt gtcatagttt tttcataaaa accgttgaca atgttttttt cctatgttat
     1921 atatgtaata taaccaaaat aggagaaatt aattatgaat gaaaaatcaa ttgaagaaat
     1981 attagctgaa agacaactaa aagaatttaa cgaatcgaaa tttggtcagt ttttcaataa
     2041 tcaaaaacta aaagaacaag aactaacata ccttaacaat aaagcattag acatagtgaa
     2101 tgaagaagat ttattcgaaa taatgtcatt aatacctcaa ccatttatta cagaagaaaa
     2161 taaaaatgtt gatgttttaa gccttatata taataacttt ttagaaaata cagaaatacc
     2221 attaacactt cctatttttt cgtttttaag ttttttaagt gctttttgtg ttaaaaatga
     2281 aatcacatat acaattccat tgagcgacga aaaatcgccg cttgatactt ggatcacgat
     2341 tttggcacct tctggaagtg cgaaaacttt ttcaaactca caaataaaaa aggttatacc
     2401 agtaggcatt gacggaaaaa aagttattga gtcaaatttc gtaaaaccta acggccctgc
     2461 gaaatttatc gaagaacttt caaatcttcc tgaaactaaa aacggcgaaa atcaatacgg
     2521 tttttggatt gaagatgaag tttcacaaat gtttaaacag gtagaacaaa tcggatctcc
     2581 actatctgaa ataaaagaat atttgttaaa atcatatgac catgaaaaat taacaaggaa
     2641 aagcaaaaaa gaaacaatcg aaactaaaaa tatagtttta actctgttat ttataaatac
     2701 atttgaaagt tatgcgaata acatttcaaa tgaatcaatg acagatggtt taatgagaag
     2761 atttaatcta atttatacag aaaaagacag tgaaagagat ttcacggatt actctttata
     2821 taacttaaaa aaaataagag atgaaacatt acaggacaaa atatctgaat tattcaactc
     2881 aataaaacca gatcaaaatt tcacattttc agacaattgt tttaaattat accaaaaata
     2941 tttcaagatt ttttggcata gaaaatataa aactatctta ccagagaaag aaaattttta
     3001 cagaacttat atgatgcaaa gctggaaatt tgccgtattt tatcatttga tcttaaagag
     3061 agaaggaacg gaaattcagg gcgattgttt ggtgtttggt atgaaaatat ctctactatt
     3121 tgccaatagt atgaaaaagt tttttgattt caaattaaat aaaaaactaa caccaaagaa
     3181 aaaagagaaa ataaaaattg attttacaaa atacattgaa tatttaaatg aaaatacaga
     3241 tattacattg agaacatttt gcagaaaatt caacctgaaa aaagctgatt caatatcaat
     3301 tttgaaaagt cttgaatgta ataattatca acacaaactt attgaagaac tggagtaatt
     3361 aacaagcgaa ttttaaattg tgccatattc aaatatattt tcgcttgttc aacccctcaa
     3421 aaataataaa gccctgacgg gcttttttat tttctcacgg ttttataaaa ggtcaaatta
     3481 tattcagttt tgcccttgac ttaaggtttt aatttgttat ctatgtaata gatagcaagt
     3541 tagagtaata cttcgtatta ctttacttgt gaggggttcc cccttcaatc cccttaaaat
     3601 caaaacctta aacccaaagg agttaaattt gagttacaga gaacgatacc aaagaaaaaa
     3661 ttttgtctca ttgtgtttga atgatgaaga attatcagag cttgaaaata tcgctgaaag
     3721 attaaatatg aaacgagcag cagcagctcg tgaaatatta ttcacgaact caaagagatt
     3781 aaaatcacaa ataaagaaaa atgataattc tgaaatcttg tttttatatt ctaaaatctc
     3841 aaacaacata aaccagatcg ca
//
