LOCUS       NZ_AP022457             4783 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STW0522-62 plasmid pSTW0522-62-5, complete
            sequence.
ACCESSION   NZ_AP022457
VERSION     NZ_AP022457.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195902
            Sequence Read Archive: DRR199707
            Assembly: GCF_015136055.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STW0522-62
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 550x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,030
            Genes (coding)                    :: 4,914
            CDSs (with protein)               :: 4,914
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 34 of 116
            Pseudo Genes (incomplete)         :: 80 of 116
            Pseudo Genes (internal stop)      :: 22 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            Pseudo Genes (short protein)      :: 1 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022457.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STW0522-62"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTW0522-62-5"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            complement(102..458)
                     /locus_tag="JLF39_RS25455"
                     /old_locus_tag="STW0522ENT62_P50010"
     CDS             complement(102..458)
                     /locus_tag="JLF39_RS25455"
                     /old_locus_tag="STW0522ENT62_P50010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(661..978)
                     /locus_tag="JLF39_RS25460"
                     /old_locus_tag="STW0522ENT62_P50020"
     CDS             complement(661..978)
                     /locus_tag="JLF39_RS25460"
                     /old_locus_tag="STW0522ENT62_P50020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1165..1407
                     /locus_tag="JLF39_RS25465"
                     /old_locus_tag="STW0522ENT62_P50030"
     CDS             1165..1407
                     /locus_tag="JLF39_RS25465"
                     /old_locus_tag="STW0522ENT62_P50030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(2078..2293)
                     /locus_tag="JLF39_RS25470"
                     /old_locus_tag="STW0522ENT62_P50050"
     CDS             complement(2078..2293)
                     /locus_tag="JLF39_RS25470"
                     /old_locus_tag="STW0522ENT62_P50050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2300..2791)
                     /locus_tag="JLF39_RS25475"
                     /old_locus_tag="STW0522ENT62_P50060"
     CDS             complement(2300..2791)
                     /locus_tag="JLF39_RS25475"
                     /old_locus_tag="STW0522ENT62_P50060"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_200925423.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2826..3530)
                     /locus_tag="JLF39_RS25985"
                     /pseudo=""
     CDS             complement(<2826..3530)
                     /locus_tag="JLF39_RS25985"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3469..3792)
                     /locus_tag="JLF39_RS25485"
                     /old_locus_tag="STW0522ENT62_P50080"
     CDS             complement(3469..3792)
                     /locus_tag="JLF39_RS25485"
                     /old_locus_tag="STW0522ENT62_P50080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4443..>4565
                     /locus_tag="JLF39_RS25990"
                     /pseudo=""
     CDS             4443..>4565
                     /locus_tag="JLF39_RS25990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(3908..4081)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          4005..4552
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.559"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(1987..3479)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3476..3792)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.45e-59"
ORIGIN
        1 taagaagaaa gttaacatga gcgtttttat ccctcaaaaa gaaggcttct agttatagaa
       61 gccttctttt tgagggataa cttatatttt tttaaaaaaa atcatgcaat agcatgtttc
      121 ttctcagaac tatcaacaat gtgtttcctt tctatgattt catgagcggg aatcactctg
      181 tctatttctt cttgcaatcg agggctacta gtaacctccc ataaatcaac agactgctgt
      241 atatttagcc agaactttgc agtggtatcg aaggctttag ccaacctaaa agccatatcg
      301 gtagacagct ttctattatt gttaactaaa gcactggcag agtttctatg cacattcaac
      361 atttcagcca agtcattgat ttttaaacca cttggttcga gatattcata aagaataata
      421 tcccctacag ttgatggttt tctagttgct tgggacatgc tttactccta aattttttac
      481 ttctctctgt tagatgagga agataagtat ttcgtctatt accagctgta gcgatgagta
      541 attatcttaa atatagtcac gtcatttttt ttattaatca ttatttgtaa tgacatttta
      601 atgtaggtga gggaacgcct cacctacttg ttttataaga taacagccac gacctgttat
      661 ctatcggtaa tcatgtggat cgagatacaa atcctcgact ccgttttcac cccacttaaa
      721 tatcaagcgg tacttctcat tcacccgtat ggatgaatac cctgtcaatg gagggttgag
      781 attctcaaat cggtttcccg ggggagaccg caaatctcca actttatcag cagcattcag
      841 catatccagc ttgcgaccta atgaagcact aatcgcagtt ggaatctttc gattggcttt
      901 accatatttg taaaactggt atagccaatc gtctcgaaac tgaatgcggc aatcatcatg
      961 ttcatgatct tgctgcattt ttcttttacc tcaatttttg ccgcaacttg gcaatttttt
     1021 cgatcacctt cttacctccg atatagtaca gcaaacatat caaatgcaca acagcacagt
     1081 gcacttgtgc aatcaatggc tatgctgtag aatgcactca tgcacagtgc aggtgtgcaa
     1141 aataaatgat aatttggagg ttgcatggct cttgttcgct caggaaacaa aagcaagatt
     1201 tttgaagcag atacgaagtt taactcggtt tttgacactg aatataagcc gggagaaaca
     1261 tgtgaatatg aagggattta taaatgtacc ggttgcggta aagaagtaac tatcgcaggc
     1321 gggaaaacaa tcccaccaca aaaccaccac caacataccc ccgcgcaagg gaacattcgt
     1381 tggcaattgt ttgtttatgc tcagtgaggt atagaaaatg gcagatcgtc gaccactcaa
     1441 cccacacgtt gtcgttccta aaccaggcgg cggaactcaa cctcgtgacc gtaacaatga
     1501 cggaagggtc agaggaaaac gctcagatgc aggaaagcca cgaaaataac agcaagccgc
     1561 cagcctggcg gcttttttat atctgcagct gatactgatt taccataaca tccgttgggc
     1621 gcaccgctga aacggggttg gctgcgtttc cggtgccaga cgcgagaaag tggcgacaga
     1681 cataacgaaa aaacaccaga aacgcagccg ctggatatta cactggtgca tagtcatgca
     1741 gccagatgca tggatgttaa agtcacacta aaatccgcgc caatgcctta aaaaccgaca
     1801 aatgcttctc cagccagtcc cacagctccg ccgccagata caagcagtta agcaatgatt
     1861 caatgagatt taacagcttc atttcccgct cctgaataag tcgtgacctt tttgatcacg
     1921 aacctattta gcgggagaaa taagagcccc tcttcgtgcg ggttgtcagc tgcctggatg
     1981 gtttaaagtg tcggcccgcg gtaggttttc tgcggctggc gctccagctc cagtgttttc
     2041 tgatactcat tctccctctg ccggagctcg atggcattca gttcccggac aagcggcgca
     2101 gctgctcgct taaactcagc acctgcgtgc tcaagtccga cacgcgctcg ttcagcaccg
     2161 cgttctcccg ttgcataagc ccacacatgc tccgccattc cgcgaaggcg ctctcccatt
     2221 cgtccagcct ttgcgagtag tcctgctgta gctgctctaa tgcgctcagc aactgctttt
     2281 ccagctctgt cattttccgc tactcctgtt tcaggcggat ccatgtcgtc ccgtcgtagg
     2341 ggatggcttc ggtttgcatc cccggcggca ggataagaaa cctccggccg tcgctgctct
     2401 cctgatagcg cactccccac gtccgggcag tcattttcgc cagggtatct tcctgctgac
     2461 gaagatgcgt gtaattgtcg gttatctgct gcccctgcca ccacagtatg ctcccgctcg
     2521 tgccgatcag cagcgctgac accatcagga tggtgaacca tgtccgcccg gcagtccgca
     2581 gtacgctccg ccggtttttc tccatcgcct cgctcatact ctgctcgtgt gcgaggatgg
     2641 cgtcgctgat tctcctcgcg cttgcgctca gttccgctct gacagactgc tcgtgctcgc
     2701 tgaatgcggc tttcagcatc tcgccggtac tctgcttctg cgctttcgat tgctgctcta
     2761 agtccttcgc cagcgttaaa agactgctca tagatggctc ccttcagtcg gatgtttcgc
     2821 cccccgtccg ggtcggcaat gctgatactg cttttcgtgg tgcgcaccac ctcaaaacct
     2881 gaccgttcca gcgcctcagt gacgtcctga cggtttttca gctccccgga cgaagcaagg
     2941 gtcagcagtc cgcgcgtgat ggcttcagtg gcctcctgct tcgttttcgg cagtccggac
     3001 ggcgtgacca gcgcccggcg gttttccggc gcgttcgggt catgcagccc cagtctgccg
     3061 ttcacgatgg tctgccaggc atcgatgcgc ggccggtcgg cgcggtcgta gtacggctgg
     3121 agccgcctgc cggtcagcag ttcggtattg gggatcagaa agttcagctc cagacgcccc
     3181 ttgtccgcgt gctccaccca cagaatgctg tactgatctt tatcgagtcc gggcatcaga
     3241 acccgctcga agctggccat cagtttttcc cgctggccgg gaggcagatc ggcctccgca
     3301 aacgacagta cgccggaggt gtatttcttc acgtaaggcg acgcgtcaat cagctcccgg
     3361 acttcttccg gcttcccctg caggacgctg gcgccttcac gctgccggtc tttacccagc
     3421 agatagtcca ccggcccggc gccaccgccg cgaccgcgtg gatgaaattt aacgatcatc
     3481 gtccgttcct ttctcaagaa cggcgtgccg cagccgctca agcccggcat cgatggccat
     3541 cagcgccgcc acaatctgta ccctgtcatg gccggtgccg ccgccggcgt taacacggcg
     3601 ggctatctgg ttcaggttat tgcccatacc ggcaagctgg cgcagcagcg ccggcgagag
     3661 tgaggggagc tttccggtgc gcgcaggctt ttcgtccagg caggtctgcc gcatccacgc
     3721 cgccagcggc ctgccgtcgc agcgctcaag cagccgccgg tgctcgccct ccgtcaccca
     3781 catggtgagc atcttgttcc gtttctctga caaaccggcc tcctgataac gccgggacgg
     3841 gcgggaaagc ctttcccggt cggcgcgggg ttggacaagc cgcaggcgcg tcagtggctt
     3901 ttggcgggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgga gtgtatactg
     3961 gcttaatatg ttgacacgga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa
     4021 aggcggcaac ggcgcgtcag cagaatatgc gatacaggat atattccgct tcctcgctca
     4081 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt gcgggcttac gaacggggcg
     4141 gaaatttcct ggaagatgcc aggaagaaac ttaacaggga agcgataagg ccacggcaga
     4201 gccggttttc cataggctcc gcccccctga cgagcatcac agaatctgac gctcaaatca
     4261 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg atggctccct
     4321 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     4381 tttgtctcat tccacgcctg acactcggtt ccgggtaggc agttcgctcc aagctggact
     4441 gtatgcacga accccccgtt cagtccgacc gctgcgcctg ttccggtaac tatcgacttg
     4501 agtccaaccc ggaaagacac gacaaaacgc cactggcagc agccactggt aacaggatta
     4561 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4621 acactggaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc aggttaagca
     4681 gttccccaac tgacttaacc ttcgaaaaac cgcctcccca ggcggttttt tcgtttacag
     4741 agcaagagat tacgcgtaca gagcacggat ctcaagaaga tcg
//
