LOCUS       NZ_AP022456             4826 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STW0522-62 plasmid pSTW0522-62-4, complete
            sequence.
ACCESSION   NZ_AP022456
VERSION     NZ_AP022456.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195902
            Sequence Read Archive: DRR199707
            Assembly: GCF_015136055.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STW0522-62
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 550x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,030
            Genes (coding)                    :: 4,914
            CDSs (with protein)               :: 4,914
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 34 of 116
            Pseudo Genes (incomplete)         :: 80 of 116
            Pseudo Genes (internal stop)      :: 22 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            Pseudo Genes (short protein)      :: 1 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022456.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STW0522-62"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTW0522-62-4"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            102..332
                     /locus_tag="JLF39_RS25415"
                     /old_locus_tag="STW0522ENT62_P40010"
     CDS             102..332
                     /locus_tag="JLF39_RS25415"
                     /old_locus_tag="STW0522ENT62_P40010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200925417.1"
                     /translation="MHEQKDKKRRLGFMIGKSNATLPPDEQTQTEAELDQEVLKLFYPN
                     QCTKDTIERSEAGTDVFTASGSDDLFKKLKL"
                     /name="hypothetical protein"
     gene            959..1225
                     /locus_tag="JLF39_RS25420"
                     /old_locus_tag="STW0522ENT62_P40020"
     CDS             959..1225
                     /locus_tag="JLF39_RS25420"
                     /old_locus_tag="STW0522ENT62_P40020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200925418.1"
                     /translation="MKIVLSVSKCLYINDLDRLLPCDRFFVHLDYKDISKKESFEMNRH
                     DQESYKLIFDKLVGNDVMLAIDEDIYSEVIENLLHHSKGVNKI"
                     /name="hypothetical protein"
     gene            1222..1398
                     /locus_tag="JLF39_RS25425"
                     /old_locus_tag="STW0522ENT62_P40030"
     CDS             1222..1398
                     /locus_tag="JLF39_RS25425"
                     /old_locus_tag="STW0522ENT62_P40030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200925419.1"
                     /translation="MMFMSVVMVILCIVLGGFMIRSCMGKTNSSSEAKQAISDVRKIID
                     KKELIKYKKRHQQ"
                     /name="hypothetical protein"
     gene            1395..1670
                     /locus_tag="JLF39_RS25430"
                     /old_locus_tag="STW0522ENT62_P40040"
     CDS             1395..1670
                     /locus_tag="JLF39_RS25430"
                     /old_locus_tag="STW0522ENT62_P40040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200925420.1"
                     /translation="MIIEMEYEMKLNVLARFFYYIEQAKDIPFYCSSYDEQSLCYFVAN
                     RYINENKADDLIQALIDTNDDDYIKAIRDYVQYTALNEVREKYEDR"
                     /name="hypothetical protein"
     gene            1657..2028
                     /gene="mobC"
                     /locus_tag="JLF39_RS25435"
                     /old_locus_tag="STW0522ENT62_P40050"
     CDS             1657..2028
                     /gene="mobC"
                     /locus_tag="JLF39_RS25435"
                     /old_locus_tag="STW0522ENT62_P40050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_200925421.1"
                     /translation="MKTDRKRGRKGLNHAERRDISIQFRVNKEEFEYLKSLVNASGLER
                     GHYIRKEILNSEPIVKRKMPDFEQSLFKEIQQQGNNINQIAKKLNTNEDFKQSLAAEAN
                     FAIKQLMKRILKACEVYTV"
                     /name="mobC"
     gene            2025..3500
                     /locus_tag="JLF39_RS25440"
                     /old_locus_tag="STW0522ENT62_P40060"
     CDS             2025..3500
                     /locus_tag="JLF39_RS25440"
                     /old_locus_tag="STW0522ENT62_P40060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_200925422.1"
                     /translation="MKGSVAKTGKSFKNRVDYILKDDHDFICSNMSSDYNNVSDLTDEF
                     KIVSSFRQDIKKPVFHAFLSLPKNEHLTDEQWQEIAKDYLKEMNIDIEKHQYICVRHND
                     TDQDHIHIVANRVGLDGSVWLGQHSAFNTIAACERLEVKHGLTITDGLKGQKSEVSAPT
                     KNEIEMALRTGEKPARLVLQASLQAAMTGKPDLETFVERLQAVGIEPRFNVASTGNVAG
                     CSFSVGDVAFKGSQLGKKFSWNTIKNKVIYDKNRDDELVRSFSARKDDEPNSIRRSLAE
                     PNINDSGAVDRIDDDIIRISQHSIKSDTTDTYESKQLVRSNNHSFKLSEKYNRPASHKV
                     RRTTQKAAQILNRVKKQYNKEPIKTYNKNGLNSWRDKSDSSSISRILDHAETANAATNN
                     FRLKGRGNINSRATKIEEIRERLEIKKPVVQPKNKEKIVKEEIKKKPLGLSMDENRFKS
                     KLKEALKKEKPKNETTPQQEDDQHLKPRGFKPR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3509..3781)
                     /locus_tag="JLF39_RS25445"
                     /old_locus_tag="STW0522ENT62_P40070"
     CDS             complement(3509..3781)
                     /locus_tag="JLF39_RS25445"
                     /old_locus_tag="STW0522ENT62_P40070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="WP_074193887.1"
                     /translation="MKVKNRKRELTAKEYAEQYEISVRTVKRYFSQDREDYEKEAKQRR
                     LKAFVLRESGMKWSDVGIALGTTKHGAIALYKRYQQIDAPTTKEA"
                     /name="plasmid replication protein"
     gene            complement(3778..4629)
                     /locus_tag="JLF39_RS25450"
                     /old_locus_tag="STW0522ENT62_P40080"
     CDS             complement(3778..4629)
                     /locus_tag="JLF39_RS25450"
                     /old_locus_tag="STW0522ENT62_P40080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_200925416.1"
                     /translation="MFKIENNKDILKSQIPLKPYATNDLEFGLTIQNKNKALDMLYLQV
                     NHPLYLHTMVFDIDKENCFYEFENAHLPIPSFITKSPDSGRCHYGYMLNAPISSTEKSR
                     QKPVKFARAVYFNMANRLGADLGYAGLITKNPLNDHWSPFWSGADLYELNDLADCFDDL
                     EEPKKRENTGLAFGRNVEMFDTIRQWAYKNVLKYQSESSFNDFHNELLLKCQMHNAYLN
                     ADDLLPYNEIKATAKSIAKFCWKEFSEEKLNKIQSKKQSYRGKKNKGIVKSKTRKFLEA
                     IK"
                     /name="replication initiation protein"
ORIGIN
        1 ccacagcgaa gcacaggccg aaggctactg cagagtaaag taacactgta acgtatacta
       61 aacttcatat gcaggatctg gctgtgctgg ggaggtaaat catgcacgaa cagaaggaca
      121 agaaacggcg gttaggtttt atgattggta agagcaatgc aacattgcca ccagacgaac
      181 aaacacaaac agaagcagag cttgatcagg aagttctgaa gctgttttat cctaatcaat
      241 gcactaaaga caccatagaa cgctcagaag ccggtacaga cgttttcacg gcctccggtt
      301 ctgatgacct gttcaaaaaa ctgaagctgt aggtgaagct gtagggcagg ggagagctgg
      361 ccatcttcac cagtagaaca aacccaaacc gacccggacg gtgagagaag gccaaccctc
      421 gatgcagtat atactaatat atactatagt ctgttgattt tatgagcatt ttaaacccat
      481 agacgccagc aaacgagcaa aagcaagttt tactcgtttc tgtcgccgcc gatcaaaccc
      541 cattccttaa aatctggtga tctgatggtc acgaactttt ccagatttca cgtagcagca
      601 gcatttttta cgcaaagcta cctattacgc acagctaccc atcaagacaa tatattattt
      661 accctatttt ttatttgcat tttatttgtt gtgcagggta tttatttatg tagccgctat
      721 attgacaaac tttttggatc ggttatttat ttaaatatag gttgagttca gctttatttt
      781 tgtttttcgg aaactgaggc aaggtgtaat agcgtatccg ctattttacc caatcctatc
      841 ggattgcctt gtctcgtttc actcgcaagg ttgaaaaaga tctacccttc cgcttattcc
      901 cctatcaagg ggaagcggta aaccatagtt agatattata aatataggag aaataaatat
      961 gaaaatagtt ttatcagtat caaaatgttt atacataaac gatttagatc gcttacttcc
     1021 gtgtgatcgt ttttttgttc atttagatta taaggatatt agcaaaaaag agtcatttga
     1081 gatgaataga cacgaccaag aaagctacaa acttatcttt gataagcttg tcggtaatga
     1141 cgtaatgctt gctattgatg aagacatcta cagcgaagtt atcgaaaact tacttcatca
     1201 ttcaaaagga gtaaataaaa tatgatgttt atgtctgtag taatggtaat actttgtatt
     1261 gttttgggtg gttttatgat tagaagctgc atgggaaaaa caaactcttc aagcgaagca
     1321 aaacaagcaa tatcagatgt tagaaaaata atagataaaa aagaacttat aaaatacaaa
     1381 aagaggcatc aacaatgatt atagaaatgg aatatgaaat gaagcttaat gtgttagcac
     1441 gtttttttta ttacatagaa caagctaaag atataccttt ttattgttca agctatgacg
     1501 aacaaagctt gtgttatttt gttgctaata gatacattaa cgaaaataaa gcagatgatc
     1561 ttatacaagc attaatagat actaatgacg atgattatat aaaagctata agagattatg
     1621 ttcagtatac agctttaaat gaagtgagag agaaatatga agacagatag aaaaagaggc
     1681 cgcaagggtt taaatcacgc agaacgcaga gatatatcta tacagtttag agtaaacaaa
     1741 gaagagtttg aatatttaaa atcattagtt aatgcatccg gccttgaaag aggccattac
     1801 attagaaaag agattttaaa ctctgaacct attgttaaaa gaaaaatgcc ggattttgaa
     1861 caatccttgt ttaaggaaat acaacagcaa ggtaataata taaatcagat tgctaaaaaa
     1921 ctaaatacca acgaagactt taaacaatct ttagccgcag aagctaactt tgccataaag
     1981 caacttatga aaagaatatt aaaagcttgc gaggtttata cagtatgaaa ggaagcgtag
     2041 ctaagacggg taagtctttt aaaaatagag ttgattacat tcttaaagat gaccacgatt
     2101 tcatatgtag caatatgtca agtgattaca ataatgtcag tgatttaact gatgagttta
     2161 aaattgtaag cagctttaga caagatataa aaaaaccggt atttcacgct tttttatctc
     2221 tccctaagaa tgaacattta acagatgagc aatggcagga gatagcaaaa gactatttga
     2281 aagaaatgaa tattgatata gaaaaacatc aatatatttg tgtgcgtcat aacgatacag
     2341 atcaagacca tatccatata gtagcaaata gagtcggttt agacggttca gtatggcttg
     2401 ggcagcactc agcatttaac acaatagcag cttgtgaacg tttagaagta aaacacggct
     2461 taaccatcac agatggctta aaaggtcaaa aatcagaggt atcagcacca accaagaacg
     2521 agatcgagat ggcattgagg acaggagaaa agccagcccg tttagttctt caagcctctc
     2581 tgcaagcagc tatgacaggt aaaccagacc ttgagacgtt cgtagaacgt ctgcaggccg
     2641 taggcattga accgaggttc aatgttgcca gcactggcaa cgtggcgggt tgttctttca
     2701 gtgtgggaga tgtggcattt aaaggctcac agttgggtaa aaaatttagt tggaacacta
     2761 taaaaaacaa ggtgatatat gacaaaaata gagatgatga actcgttagg agcttctcag
     2821 caagaaaaga tgatgaacca aatagtatta gacgatcact tgctgaacca aatatcaatg
     2881 acagtggagc cgttgacaga atcgatgatg atattatccg aatcagtcag cacagcatta
     2941 aatcagacac aacagacact tacgagagta aacaactcgt tagaagcaac aaccacagct
     3001 ttaaactcag cgaaaaatat aatagaccag caagccataa agttagaaga acaacacaaa
     3061 aagctgcaca aatactcaac agagttaaaa aacaatacaa taaagaacca atcaaaactt
     3121 ataataaaaa tggcctcaat agttggcgtg ataaatctga tagcagttct atcagtagga
     3181 tacttgatca tgcagaaaca gccaacgcag ccacgaataa ctttagactc aaaggccgtg
     3241 gcaacatcaa tagtagagca actaagatcg aagagataag agaaaggctt gaaataaaaa
     3301 aaccagttgt tcagccaaag aataaagaaa aaatagttaa agaagaaata aagaaaaagc
     3361 cgttaggttt gagtatggat gaaaatagat ttaaatcgaa gttaaaagaa gctcttaaaa
     3421 aagaaaaacc aaaaaatgaa acaacaccac agcaggaaga cgatcagcat ttaaaaccgc
     3481 gtggttttaa accaagataa aagaggcttt aagcctcttt tgttgttggt gcgtctattt
     3541 gttgataacg tttataaaga gctatagcac catgttttgt agttccaaga gcaataccaa
     3601 catcagacca tttcataccg ctttcacgta atacaaaagc ttttaatcgt ctttgttttg
     3661 cttctttttc atagtcttca cgatcttgag agaaatatct ttttactgtt ctaacgctaa
     3721 tctcatactg ttcagcatac tcttttgcag taagttctct ttttctattt ttaactttca
     3781 ttttatggcc tccaaaaact tacgagtttt actttttact attcctttgt ttttctttcc
     3841 acggtaagac tgttttttac tctgtatctt gttgagtttt tcttcgctga attctttcca
     3901 acaaaacttt gcaatgcttt ttgctgtggc ttttatctca ttatatggca gtaaatcatc
     3961 agcatttaaa tatgcattat gcatttgaca ctttagcaat agttcattgt gaaaatcatt
     4021 aaatgagctt tcactctgat attttagcac atttttataa gcccactgcc ttattgtgtc
     4081 gaacatttca acattacgac caaaagcaag ccccgtattt tctcgttttt tgggttcttc
     4141 taaatcatca aaacaatcag ctaaatcatt taactcatat aaatcagcac ctgaccagaa
     4201 cggcgaccag tgatcattga gtgggttttt tgtaatcagt ccggcatacc ccagatcagc
     4261 acctaaacgg ttagccatat taaaataaac agctctggca aactttacag gcttctgtct
     4321 gcttttctcc gtagaggata taggagcatt aagcatataa ccataatgac aacgtccact
     4381 atcagggctt tttgttataa aagacgggat gggcaaatga gcattttcaa actcgtaaaa
     4441 gcagttttct ttgtctatat caaataccat tgtatgaaga taaagcgggt gatttacttg
     4501 taaataaagc atatccaagg ctttattctt attttgaata gtcaatccga actctaaatc
     4561 atttgtagca tatggcttta aaggtatttg actttttaat atatctttgt tattttctat
     4621 cttgaacata tagcagtatc ctttctgtaa tgccccgttt cttgttggtc gccaaacttt
     4681 ccaagaaacg cgggcttttc ttttatttat aatctcttac agttttacta tatcaaataa
     4741 tcttgtcaat agatccatca aaaaaaagtt gttatgtgtg ccattaacca atatcaggta
     4801 ttagacggag ttttggggac cctaca
//
