LOCUS       NZ_AP022454             6183 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STW0522-62 plasmid pSTW0522-62-2, complete
            sequence.
ACCESSION   NZ_AP022454
VERSION     NZ_AP022454.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195902
            Sequence Read Archive: DRR199707
            Assembly: GCF_015136055.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STW0522-62
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 550x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:24:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,030
            Genes (coding)                    :: 4,914
            CDSs (with protein)               :: 4,914
            Genes (RNA)                       :: 113
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 34 of 116
            Pseudo Genes (incomplete)         :: 80 of 116
            Pseudo Genes (internal stop)      :: 22 of 116
            Pseudo Genes (multiple problems)  :: 16 of 116
            Pseudo Genes (short protein)      :: 1 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022454.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STW0522-62"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTW0522-62-2"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            102..1013
                     /locus_tag="JLF39_RS25340"
                     /old_locus_tag="STW0522ENT62_P20010"
     CDS             102..1013
                     /locus_tag="JLF39_RS25340"
                     /old_locus_tag="STW0522ENT62_P20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004088970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_200925413.1"
                     /translation="MTNQALDLFNERLPRKPYFTDELQFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRPGAAIDWSDRNAPAPTLTIMNPENGHAHLLYALKTAVRTAPDGKMK
                     PLKYAAAVERALCQKLDADTGYSGLICKNPNHDHWQLAVWQPELYTLDWLADSLDLSAA
                     NDKEILPDYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLKACYERTCAYNLQFLTPLD
                     ENEVAGIAKSIAKWTHKNFSEESFSDFVLKTHTSELQSKRGRIGGAIGGKISKGGGRPS
                     LGEPWISLGISRRKFFYDKRNK"
                     /name="replication initiation protein"
     gene            1167..3095
                     /locus_tag="JLF39_RS25345"
                     /old_locus_tag="STW0522ENT62_P20020"
     CDS             1167..3095
                     /locus_tag="JLF39_RS25345"
                     /old_locus_tag="STW0522ENT62_P20020"
                     /inference="COORDINATES: protein motif:HMM:NF013080.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="WP_200925414.1"
                     /translation="MIKLKKTFILSIYFLLMSTILAYGLGYFKKPGVISYIMIAGFFIC
                     YFVSSRSKLCSIMLFTIFSISSIYFPTGITYGLLSKDIMLPLIGTNSMEIFGYILAMKY
                     YFGISILFVILNIPFIFMKKKGGSSLVYIFFIFLISFVMYGFSNSNVYLNDHGIIRVAL
                     RSIIEINHDNIDFYKELNDNSKLKSERSNKNFDPEIRILVIGESVRRDYMSLYGYPYQT
                     TPYLDSSNGIFIDGMTSAAPNTEQSLGRALFKTYPDQNKIDHGINIISLAKKAGYLTYW
                     VSNQGRSSNGDSKLYKLSKLGNESYFMKKGDFLSSHEGDEDMLPIIEKIVRSNDNKKVI
                     IIHMMGSHEPVCSRLTNFTPQFKINNEAGCYVASIEKLDGFIKNIISIVNGKSYKLMYF
                     SDHGLYVDKDKIFHDVDLYDEYQVPFFYLSSNMKSRVLLNKKVSGLNILDFYASFINVK
                     SNYTDSIYNIEDYNKIPESKDPIIYWVNYKKLSALHEKQRPLNPSYILGQDGINEHILN
                     INKPISATNNCIGYIDNYSYLYPSNKSITLTQGWIASVQDKKPVIGRVGLIINHNGNYS
                     FIEGSRVNRIDVYNALNNLDPSNIEYGISVKAKKRENIEMYLGYINNENKAYICTNHAP
                     LIREFGQ"
                     /name="phosphoethanolamine transferase"
     gene            3193..>3303
                     /locus_tag="JLF39_RS25890"
                     /pseudo=""
     CDS             3193..>3303
                     /locus_tag="JLF39_RS25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041205629.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     gene            3566..3901
                     /gene="mobC"
                     /locus_tag="JLF39_RS25350"
                     /old_locus_tag="STW0522ENT62_P20030"
     CDS             3566..3901
                     /gene="mobC"
                     /locus_tag="JLF39_RS25350"
                     /old_locus_tag="STW0522ENT62_P20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_080202507.1"
                     /translation="MDKRTKEIKIRLTESEHERLLARCDRAHLAEWLRSLGLGEHVLRR
                     RRVPDVDPALLRQVTGIGNNLNQIARKLHGLDAIPAYQQVSLLAVLTSLDRQLSELLET
                     QRDRQNS"
                     /name="mobC"
     gene            4101..>4523
                     /locus_tag="JLF39_RS25895"
                     /pseudo=""
     CDS             4101..>4523
                     /locus_tag="JLF39_RS25895"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4570..5055
                     /locus_tag="JLF39_RS25360"
                     /old_locus_tag="STW0522ENT62_P20050"
     CDS             4570..5055
                     /locus_tag="JLF39_RS25360"
                     /old_locus_tag="STW0522ENT62_P20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_200925412.1"
                     /translation="MSGILDLAKAFEETSKQQAQSTGESVKSEFVKLEQSISEALKSSG
                     KNISDAIHEQNTRQTTLLRRSVTIALIWILVCGVLLIGILSGILWFQGTLIASRLDEMN
                     SYSQQLTDLKAKSGAGVEILTDDTRKNKYYVVLPKSAKGVELYTSKNDKQVVTYTAK"
                     /name="MbeB family mobilization protein"
     gene            5116..5352
                     /locus_tag="JLF39_RS25365"
                     /old_locus_tag="STW0522ENT62_P20060"
     CDS             5116..5352
                     /locus_tag="JLF39_RS25365"
                     /old_locus_tag="STW0522ENT62_P20060"
                     /inference="COORDINATES: protein motif:HMM:NF016769.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_037427059.1"
                     /translation="MTETEKLLLSALERLQDDYETRQQAWQASYDSLQTMFTTTSAALM
                     RSDQACQHLSGQVESLRQQVMSLTEAVSRLTRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5456..5764)
                     /locus_tag="JLF39_RS25370"
                     /old_locus_tag="STW0522ENT62_P20070"
     CDS             complement(5456..5764)
                     /locus_tag="JLF39_RS25370"
                     /old_locus_tag="STW0522ENT62_P20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_037427062.1"
                     /translation="MQFMVYRNNSNNRAYPYLLDVQSDIIDELNTRLVIPLFLLDEVNG
                     RPARRLTPVLNVDGEDFLVMTHEMASVRQSQLGEEVVSVREHRQAIKNALDFLLDGF"
                     /name="CcdB family protein"
     gene            complement(5764..6003)
                     /locus_tag="JLF39_RS25375"
                     /old_locus_tag="STW0522ENT62_P20080"
     CDS             complement(5764..6003)
                     /locus_tag="JLF39_RS25375"
                     /old_locus_tag="STW0522ENT62_P20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_064179603.1"
                     /translation="MPVSAMRHKKTVSVTIEPALLHQAREAGINLSATLTTALKHQIRE
                     SEAERWKRANRKAIQELNRITDEHGLLSDDHRTF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
ORIGIN
        1 ggcggaagga catgattaac gagggcgcag gcacaaaacc taagaaggat actagcacat
       61 gaaccgtgcg aaacaacagc ccgccataag cgcaggcgag attgactaac caggcactcg
      121 acctttttaa cgagcggctt ccccgcaaac cctattttac cgacgaactc cagttcggcg
      181 tacgcattgc cgggaaagag cgcgccattc ttgcgaaata tatccagttc aaccagccac
      241 acgcgatgtt ctggctcggg tttgacgttg accgccctgg tgcggcgata gactggagcg
      301 accgcaacgc ccccgcgccc acactgacca tcatgaatcc cgaaaatggt cacgcccatt
      361 tgctgtacgc actgaaaacc gcagtacgca ccgcacctga cggaaaaatg aagccgctga
      421 agtatgcggc agcggttgag cgtgcgctgt gtcaaaaact ggacgctgac accggctatt
      481 cggggttaat ctgcaagaat ccgaaccatg accactggca gcttgccgtg tggcagccgg
      541 aactttacac gctggactgg ttagcggact cactcgatct gagtgcggcg aacgacaaag
      601 aaatcctacc cgattatggt ttaggccgta actgcaccct gttcgataaa acccgtaaat
      661 gggcgtatcg cgcaatccgt cagggctggc ctgagtatga tcagtggctg aaagcctgtt
      721 acgaacgtac ctgtgcttat aatctgcaat ttctaacccc attagatgaa aatgaggtgg
      781 ctggaatagc taaaagcatt gctaagtgga ctcataaaaa tttttcagag gaatcatttt
      841 cagattttgt actgaaaact catacctccg agttgcaatc aaaacgaggt cgtattggag
      901 gagctattgg aggtaaaata tctaaaggag gtggacgacc atcactggga gaaccatgga
      961 tttcattagg cattagtagg cgtaagtttt tttatgacaa acgaaataag taagtggatt
     1021 tataaaatat tatcagggat gcttgaggta aacattattg tggcgttgtt taataaatca
     1081 gattcgaggc taacatctct gtagctgaca gcttttcaga gaacttacta tctttagtta
     1141 ttattaattg ttgagatttc aatcggatga ttaaattaaa aaaaacattt attttatcaa
     1201 tatatttctt actaatgagt accatacttg cttatggtct tggttacttt aaaaagccag
     1261 gtgtcatatc atatataatg atagctggct tttttatttg ctactttgtt tcatcgagga
     1321 gtaaattatg ctcgataatg ctttttacta tattctcaat atcctcgatt tatttcccaa
     1381 caggaataac atatggtctt ttaagtaagg atataatgct tcctcttatt ggaacaaatt
     1441 ccatggagat atttggttat atacttgcca tgaagtacta ttttggaatt tcaattctat
     1501 ttgtgatttt aaatatacca tttattttca tgaaaaagaa aggtggaagc tcgctcgtat
     1561 acattttttt tattttttta atttctttcg tgatgtacgg tttctcaaat tcaaatgtat
     1621 atttgaatga tcatgggata attagagttg cattaaggtc aattattgag ataaatcatg
     1681 acaatattga tttttataaa gagctaaatg acaatagtaa attaaaatct gagaggtcaa
     1741 ataaaaactt tgatccagag attagaatct tagttatagg ggagtcagtt cgtagagatt
     1801 acatgtctct ttatggttac ccatatcaaa cgactcctta cttagattca tcaaatggta
     1861 tttttattga tggaatgaca tcagctgccc caaatacaga acaatcactt ggcagagctt
     1921 tgtttaaaac atatcctgat caaaataaaa tagaccatgg cataaatatc atctcattag
     1981 caaaaaaagc tgggtattta acgtactggg ttagtaatca aggtagatca tcaaatggtg
     2041 atagtaagct ttataaatta tcaaaattag gtaatgaatc atactttatg aaaaaaggag
     2101 attttctttc aagccatgaa ggcgatgaag acatgctccc aataatagaa aaaatagtca
     2161 gaagtaatga taataaaaag gtaataataa tacatatgat ggggtcacat gaaccagtat
     2221 gctcaaggct aacaaatttt acgcctcagt tcaaaataaa taatgaagca ggatgctatg
     2281 tagcctcaat tgagaagctt gatggattca tcaaaaatat aataagtatt gttaatggga
     2341 aaagttataa attaatgtat ttctcagatc atggtttgta tgtggacaag gataaaatat
     2401 ttcacgatgt agacctatat gatgagtacc aagttccttt cttttattta tcttcaaata
     2461 tgaaaagtag agttcttctt aataagaaag tgagtggttt gaatatatta gatttttacg
     2521 catcatttat aaatgtaaaa agcaattata cagacagtat ttataatatt gaagattata
     2581 ataaaatacc agaatcaaaa gatcctatca tttattgggt taattacaaa aaactatcag
     2641 cactacatga aaaacaaaga cctttgaacc caagctatat acttggacaa gatggcatta
     2701 atgagcatat tttaaatata aataaaccga tttctgcgac aaataattgc attggctaca
     2761 ttgataacta ttcatatcta tacccatcaa acaaatcaat tactcttact caaggatgga
     2821 ttgcatcagt gcaagataag aaacccgtta tcggtagagt gggcttgatt attaatcata
     2881 atggtaatta ttcttttata gaaggctcaa gagtaaacag aatcgatgtt tataatgcgt
     2941 tgaataacct agacccatca aacatagagt atggtattag tgttaaagct aaaaaaagag
     3001 aaaatattga aatgtatcta ggttatataa ataacgaaaa taaagcttat atatgtacta
     3061 atcatgcgcc actgatacgc gaattcggac aataagtgca acgctctgtc atcatgacag
     3121 tggagaatgt aggcggaagg ggtaactacc gataggctat cccattcctc ctaccacaga
     3181 tgagcgagta acatgaagta acggcagtta actgaagtcg tacgatacca gataggactg
     3241 ttgtttggtg agaagattag tctgactgag atcagtaagc gtgttggcat cagtaaatct
     3301 acatgtcatc cctagcatgg atcaggtgaa ttttgcacta aacaaagcct atatcagata
     3361 acagccctct ttaaagaggg cttttttttt gcagccagca tagaatcttt catactgcat
     3421 tatagattat agccagtaca cacagcgtcc cctctgcgag ggttccttct gtgactggtt
     3481 cagggggctc gccgcccccc gaaacccctg gcatccactg cgaaaattcg cactctgggt
     3541 gcgaaagttt tctcagcgga gtgtcatgga caagcgaacg aaagagataa aaatccgcct
     3601 gactgagagt gaacatgagc gactactggc acgttgtgat cgtgctcatc tcgctgaatg
     3661 gctgcgctcg ctcggtctgg gtgaacatgt attacgtcgt cggcgtgtgc ctgatgtaga
     3721 tccggcactg cttcgccagg tgaccggcat cggaaataac cttaatcaga tagcccgtaa
     3781 gctgcatggt ctggacgcta tcccggccta tcagcaggtc tctcttctgg cggtactgac
     3841 cagtcttgat cgccagttat ctgagcttct ggagacacag cgtgatcgtc aaaattcata
     3901 gccggggtgc gggtgcaggc agtggcccgg tggattacct gctggggaaa gaacgagaca
     3961 gggaaaaagc ggtggtgctg cgcggtgagc ctgagcgtat caggcaactg attgatggtt
     4021 gtgattttgc ccgcgcctac acctccgggg tgttgtcgtt ccgggaagaa aacctccctg
     4081 acgatgagaa agagcgtctg atggaggagt tcgagcagac actgttgccc ggtctggatg
     4141 gcgaccagta cgcctgcctt tgggtggagc accgggacaa ggacaggctg gagctgaatt
     4201 ttgtgatccc gaacatcgaa ttgcagagct ggaaacgcct gcaaccctac tatgaccggg
     4261 ctgaccgtcc cagggtcaat gcctggcaga ctctgaccaa tgaccgcctg aatcttgatg
     4321 accctcatga tcccgcccga aggcgaaccc tgacaccggg aaatgacctt cccggcgacc
     4381 gtcagaaggc ggcgcagacc attacggact cgctggaaac gatggtggca caggggatga
     4441 tccgcgaccg tgaggatatg gtcagggcgc tggaaaacgg cgggttgaca gtggtgcgag
     4501 aaacgaaaag cagtctcagc attgccgacc cggacggtgg acgaaatatc cggttaaaag
     4561 gagcgttata tgagcgggat tttagattta gcgaaagcct tcgaggagac ctcgaagcag
     4621 caggcacaga gtaccgggga gagcgtgaaa agcgagtttg tgaagctcga gcaatccatc
     4681 agcgaggcct tgaaatcaag cgggaagaac atcagcgacg ctatccacga gcagaacacc
     4741 cggcagacaa ccctgctgcg gcgctcggtc acgatagcgc tgatatggat actggtttgt
     4801 ggtgtgctgc tgatcggtat tctgtcgggg atactgtggt ttcaggggac actcatcgcc
     4861 tcacggctgg acgagatgaa cagctacagc cagcagctta ccgacctgaa agcgaaatcg
     4921 ggggcggggg tggaaatact cacggacgac acccggaaga acaagtatta cgtggtgctg
     4981 ccgaagagcg cgaagggcgt cgagctgtac acctcgaaaa acgacaaaca ggtagtgacc
     5041 tacaccgcga aatagccccg tcagttgcca cagaagcgcc atacggcgtt tttagcgata
     5101 aggacaagca aatccatgac cgagaccgaa aaactgctgc tgagtgcgtt agagcgctta
     5161 caggacgatt acgagacacg acagcaggca tggcaggcca gttacgacag cttgcagacc
     5221 atgttcacca ctacttccgc cgcgctgatg cgcagcgacc aggcgtgtca gcacttgagc
     5281 gggcaggtgg agagcttgcg gcagcaggtc atgagcttaa ccgaagccgt cagccgcttg
     5341 acgcgcaggt agccagactg gacagggagc tggcgcagaa acaggaacga cagaaaacgc
     5401 gctcatttgc ccccggacgc agccggggac acgatcacgg gatggaaagg taaactcaga
     5461 aaccgtcgag cagaaaatcc agcgcattct tgatggcctg ccggtgctcc cggacgctca
     5521 ccacctcttc ccctagctgt gactggcgaa cgctggccat ttcatgtgtc atgaccagaa
     5581 aatcctcacc gtccacattc agcaccgggg tcaggcggcg ggcgggccgt ccattcactt
     5641 catccagcag aaacagcggg atcaccaggc gggtattcag ttcgtcgata atatcgctct
     5701 gtacatcgag cagataggga taagcccggt tgttgctgtt attgcggtaa accatgaact
     5761 gcatcagaac gtcctgtgat cgtcagagag taagccgtgc tcgtccgtaa tacggttgag
     5821 ctcctggatt gccttgcggt tggcgcgttt ccagcgctcc gcttctgact cgcggatctg
     5881 atgtttcagg gcggtggtga gcgtggcaga cagattaatc ccggcttcgc gggcctgatg
     5941 caaaagcgca ggctcgattg tcacactgac ggttttttta tggcgcatcg ctgatacggg
     6001 catgatgatc tcctgatttt tacacgtgta tatagcacgt gttgccagtg tacgcgtttt
     6061 ttacccggaa ggtaatggtc tgttgcttca ggcaataact gacggtgaca ggtgacaaaa
     6121 tacggcgggg tgtgtaacgt taagcggcag aaaagacgac gcccccgtaa tcatgctcga
     6181 ctt
//
