LOCUS       NZ_AP022451             2214 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterobacter kobei strain STW0522-60 plasmid pSTW0522-60-5, complete
            sequence.
ACCESSION   NZ_AP022451
VERSION     NZ_AP022451.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195900
            Sequence Read Archive: DRR199705
            Assembly: GCF_015135855.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter kobei str. STW0522-60
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 409x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:22:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,372
            CDSs (total)                      :: 5,255
            Genes (coding)                    :: 5,104
            CDSs (with protein)               :: 5,104
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 36 of 151
            Pseudo Genes (incomplete)         :: 112 of 151
            Pseudo Genes (internal stop)      :: 23 of 151
            Pseudo Genes (multiple problems)  :: 19 of 151
            Pseudo Genes (short protein)      :: 1 of 151
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022451.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="STW0522-60"
                     /isolation_source="sewage"
                     /db_xref="taxon:208224"
                     /plasmid="pSTW0522-60-5"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            complement(102..338)
                     /locus_tag="JLF31_RS26740"
                     /old_locus_tag="STW0522ENT60_P50010"
     CDS             complement(102..338)
                     /locus_tag="JLF31_RS26740"
                     /old_locus_tag="STW0522ENT60_P50010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001676031.1"
                     /translation="MNRLTNLTPAEKKFLDDAIAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSEFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            1037..1168
                     /locus_tag="JLF31_RS26745"
     CDS             1037..1168
                     /locus_tag="JLF31_RS26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032159035.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDKAPLAAAIGNWISRAG"
                     /name="hypothetical protein"
     gene            1624..2049
                     /locus_tag="JLF31_RS26750"
                     /old_locus_tag="STW0522ENT60_P50020"
     CDS             1624..2049
                     /locus_tag="JLF31_RS26750"
                     /old_locus_tag="STW0522ENT60_P50020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_001676030.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIVPVLLRVWSTELALTNQRVIAKVGLIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMDSKQQ"
                     /name="PH domain-containing protein"
     Typing          complement(503..676)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          600..1146
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.727"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 aaacgcccct gatgatttct gcgcgtctgc atggctattc acaggagtga atggcgggca
       61 atttcggagt gtcagcacca ctaaaccgcc aaaaggcgca atcagcggcg cggcgcgcgc
      121 ggacgtgacc aggcaaactc ggactgctgc cgctcatcct cacgtaatgc acgctggcgg
      181 tcggcgtaac gctgcgactc aatctgcgca cgcgctctgt tcaggacgat atgccggttc
      241 ggctgattta gctttttgcc tgacgctctc tcagctgcgg caatagcatc atcaagaaat
      301 tttttctctg caggtgtcag gtttgtcagt ctgttcatag cattctccca tcagaataac
      361 gccgcacagt ctgccagaca cagcagaacc gatgaagcgc tggcggtgct gaaagacagc
      421 cagaaacggt gaacgggagc ggattttaat ccggtgacgt gagacgttgg gcaagccgca
      481 ggcgcgtcag tggttttagc gggtttcggg gcgcagccct gaaccagtca cgcagcgcta
      541 gcggagtgta tactggctta ctatgttggc acggatgcga atatcagtga agtgcttcat
      601 gtggcaggag aaaaaaggcg gcaccggcgc gtcagcagaa tatgcgatac aggatatatt
      661 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtgcggg
      721 cttactggcg gggcggaaat ttcctggaag atgccaggaa gaaacttaac agggaagcga
      781 gaaggccacg gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat
      841 ctgacgctca aatcagtggt ggcgaaaccc gacaggactt aaagatacca ggcgtttccc
      901 cctggtggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
      961 ctgttatggc cgcgtttatc tcattccacg cctgacactc ggttccggga aggcagttcg
     1021 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gactgctgcg ccttttccgg
     1081 taactatcaa cttgagtcca acccggaaag acacgacaaa gcgccactgg cagcagccat
     1141 tggtaactgg attagcagag cgggataatg taggcagtgc tacagagttc ttgaagtggt
     1201 ggcctgagtg cggctacact ggaaggacag tttaggtatc tgcgctctgc tgaagccagt
     1261 taccacggtt agaaaatctg ccaggattta ctaaccttcg aaaaaccacc tgccagggtg
     1321 gttttttcgt ttccaaggca agagattacc cgcagagaaa gacgatctca agaagatcat
     1381 ctgtgatatt cgacagatat gggataaagg tttctaacgc caggagtata tggggcgtag
     1441 cagcagcgtt caccaccgtt gaatcaagct actttcatgg caagaaataa aagaaccgag
     1501 cagtccgaga gtcgctacgc tccccttaat ttttgtatat ctaccacgcg gatataaggt
     1561 ttctattgcc gattcatctt aaaaagggaa aaatcctatt tttttctaaa tggatgtaac
     1621 cttatgggaa gctacgtaga ccaatcattg acgagaaatg aatctgtaat atctcgcgct
     1681 caaacctcct ggattccaac aatcatccca gtaattatcg gaatcctgtt gctacctttt
     1741 tacggcttag gattacttat aatagtccct gtgcttctta gagtttggtc aacggagctt
     1801 gcattgacta accaaagagt catcgcaaaa gtagggttga tccgacgtaa tacggtggaa
     1861 ctccgtattg ataaagttga aagccttggc atacatcaag ggatactagg acgaatcttt
     1921 aattatgggt cgatcgtaat taaaggtacc ggcggaacca acacgccaat tccaaacatc
     1981 aaggcaccaa tgcaattcag atctattgtg aacaatcaca ttgaggaaat ggattctaag
     2041 cagcagtagt ttcctcataa gcattacaaa aaaagccccg cagggctttt tttgtacctg
     2101 ccagtgagtc agatttacca taacatccgt tgtctgcact gcctgagagg ccctcagcgc
     2161 gtttctgcgg cctgacagcc gtttccggca cctttcaccg caccaacccc tgaa
//
