LOCUS       NZ_AP022418             2723 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain STW0522-39 plasmid pSTW0522-39-6,
            complete sequence.
ACCESSION   NZ_AP022418
VERSION     NZ_AP022418.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195879
            Sequence Read Archive: DRR199684
            Assembly: GCF_015135515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            STW0522-39 isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 288x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 10:03:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,526
            CDSs (total)                      :: 5,415
            Genes (coding)                    :: 5,260
            CDSs (with protein)               :: 5,260
            Genes (RNA)                       :: 111
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 63 of 155
            Pseudo Genes (incomplete)         :: 82 of 155
            Pseudo Genes (internal stop)      :: 38 of 155
            Pseudo Genes (multiple problems)  :: 23 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022418.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="STW0522-39"
                     /isolation_source="sewage"
                     /db_xref="taxon:1463165"
                     /plasmid="pSTW0522-39-6"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            complement(102..338)
                     /locus_tag="JLF46_RS27460"
                     /old_locus_tag="STW0522KLE39_P60010"
     CDS             complement(102..338)
                     /locus_tag="JLF46_RS27460"
                     /old_locus_tag="STW0522KLE39_P60010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001676031.1"
                     /translation="MNRLTNLTPAEKKFLDDAIAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSEFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            737..1519
                     /locus_tag="JLF46_RS27465"
                     /old_locus_tag="STW0522KLE39_P60020"
     CDS             737..1519
                     /locus_tag="JLF46_RS27465"
                     /old_locus_tag="STW0522KLE39_P60020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080901937.1"
                     /translation="MENHYEYLKLDMSASIDEIRDAIELNKNNDEARIRKIKSILLNER
                     FKSEYDEKLISYLINNGDSSRKADEKKQSLYIEQIKNLDNGELHNSYIYAAFFLLVLNF
                     SLSLVVSTQVDYVISFIVNILTIFVLYKDWKILEKNNLAVFSKWWVLISPVYLFKRSNA
                     KKESKKLFSLWMAIIIIFVASSLIFAGGKSTVESAACGVVSDIYRTQFHQPGLTCKSVT
                     ITESEGKNHYGIAEISDGSVKDITIKELSDGQIYVTVE"
                     /name="hypothetical protein"
     gene            complement(1549..2442)
                     /locus_tag="JLF46_RS27470"
                     /old_locus_tag="STW0522KLE39_P60030"
     CDS             complement(1549..2442)
                     /locus_tag="JLF46_RS27470"
                     /old_locus_tag="STW0522KLE39_P60030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_080816845.1"
                     /translation="MHNAALGLFNDRLPHKPYFSDDLHFGVRIAGKERALLAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPENGHAHLLYALETSIRTAPDGRMK
                     PLKYAAAVENALRKKLDADTGYSGLLCKNPNHGHWKIAVWQPELYTLDWLADFLDLNAA
                     NNKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERASAYNLQFSSPLD
                     DNEVIGIAKSIAKWTRANFSEGTFSDYVFRTHSSEIQSLRGRKSKGGGRPSLNEVWVDL
                     GVSRRTYFRMKSKLKG"
                     /name="replication initiation protein"
ORIGIN
        1 aaacgcccct gatgatttct gcgtacctgc atggctatgc acgcgggtga atggcagacg
       61 atttcggagt gtcagcacca ctaaaccgcc aaaaggcgca atcagcggcg cggcgcgcgc
      121 ggacgtgacc aggcaaactc ggactgctgc cgctcatcct cacgtaatgc acgctggcgg
      181 tcggcgtaac gctgcgactc aatctgcgca cgcgctctgt tcaggacgat atgccggttc
      241 ggctgattta gctttttgcc tgacgctctc tcagctgcgg caatagcatc atcaagaaat
      301 tttttctctg caggtgtcag gtttgtcagt ctgttcatag cattctcccg tcagaataac
      361 tccgcacagt ctgccagaca cagcagaacc gatgaagcgc tggcggtgct gaaagacagc
      421 cagaaacggt gaacgggagc ggattttaat ccggtgacgt gagacgttgg gcaagccgca
      481 ggcgcggcag tggttttagc gggtttcggg gcgcagccct gaaccagtca cgtagcgcta
      541 gcggagtgta tactggctta atggtgacgc tgcgctccca tgactgagta ggaattgctg
      601 caaaaaacag ccatctgaca aaagagggct gttatctgat ataggttgtt tagtgccaac
      661 tgccgagaac cctttttaag actattctta atgcgtataa tcaacctttt agaaggtact
      721 tactgcaagg aattacatgg aaaatcatta cgaatacctg aaacttgaca tgtctgcttc
      781 aatcgatgaa atcagagatg ctattgagct aaacaaaaat aatgatgaag ctagaataag
      841 gaagataaaa tcaatcttac ttaatgagcg attcaaaagt gaatatgatg agaaattaat
      901 atcatacttg ataaataatg gtgactcatc aagaaaagca gatgaaaaaa aacaaagttt
      961 atacattgaa cagataaaaa acttagataa tggggagttg cataactcat acatatacgc
     1021 cgcgtttttc ctgttggttt taaacttctc tttatcatta gtggttagca cacaagtaga
     1081 ctatgtaatt agtttcatag tgaacatatt gaccattttt gtgctttata aagactggaa
     1141 aatattagaa aaaaacaacc ttgctgtatt ttctaagtgg tgggtattaa tatcacctgt
     1201 ttatttattt aaaagatcca atgcaaagaa agaaagtaaa aaactcttct ctttgtggat
     1261 ggcgataatc attatttttg ttgctagtag cttaatcttt gctggcggta agtctacagt
     1321 ggaaagtgca gcctgtggag tcgtaagcga catatacaga acacagttcc atcaaccagg
     1381 actaacttgc aaatctgtga cgatcacaga aagtgaaggg aaaaatcact atggcatagc
     1441 tgagatatct gacggatctg taaaagatat cactatcaaa gaacttagtg atgggcaaat
     1501 atacgtcact gttgaataac aaagttagtg gctggttgat cccagccact aaccttttag
     1561 ttttgacttc attctgaaat atgttcttct gctaacaccc aaatcaaccc acacttcatt
     1621 taatgacggc ctccccccac ctttgctttt cctccctcgg aggctttgta tttcagatga
     1681 atgcgtacga aaaacataat cactgaaagt accttcacta aaattagcac gagtccattt
     1741 cgcaatactt ttagctatgc caatcacctc attatcatcc agaggcgatg aaaattgcag
     1801 gttgtaagca ctggcacgtt catagcaagc ttgcagccac tggtcatatt caggccagcc
     1861 ctgacggata gcgcgataag cccacttccg tgttttatcg aacagagtgc agttgcggcc
     1921 taagccatag tcggcaacga tctctttgtt gtttgccgcg ttcagatcga ggaaatcagc
     1981 aagccagtcc agcgtgtaga gttctggctg ccaaacggca attttccagt gaccgtggtt
     2041 cggattcttg cagagcaggc cagaataccc tgtatcagca tccagttttt tacgcagcgc
     2101 gttttccacg gcagctgcat acttcagagg tttcattctg ccgtccggcg cagtacgtat
     2161 cgaggtttcc agtgcgtata gcaggtgcgc gtgcccgttt tccgggttgg tgatggtcag
     2221 cgtgggcgcg ggcgcgttcc ggtcactcca gtcgatggcc gcaccggcgc gatcgacgtc
     2281 gaagcccagc cagaacatgg catgcggctg attaaactgg atgtactttg ccaagagtgc
     2341 gcgttctttc cctgcgatac gcacaccgaa gtgcagatcg tccgagaagt acggcttgtg
     2401 cgggagtcgg tcgttaaaaa gtccgagtgc ggcgttatgc aatgcgccct acgcttatgg
     2461 cgggctgttt tctggcacgg ttcatgtgct aatatccttt caaggtttcg acctagccct
     2521 ggtaatcatg cgatccgcca agatatgaca tgatttccgg ggctttgttt tttctggggt
     2581 taacgttaca ccgtccgcag gcttctgtcg atctcaccac gatttaccat aacatccgtt
     2641 ggccgcaccg cctcaaacgc cctcagcgcg tttctgaggc ctccggcctg aaacatacac
     2701 ctttctgtgc ccatatcgag aaa
//
