LOCUS       NZ_AP022417             3338 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain STW0522-39 plasmid pSTW0522-39-5,
            complete sequence.
ACCESSION   NZ_AP022417
VERSION     NZ_AP022417.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195879
            Sequence Read Archive: DRR199684
            Assembly: GCF_015135515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            STW0522-39 isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 288x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 10:03:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,526
            CDSs (total)                      :: 5,415
            Genes (coding)                    :: 5,260
            CDSs (with protein)               :: 5,260
            Genes (RNA)                       :: 111
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 63 of 155
            Pseudo Genes (incomplete)         :: 82 of 155
            Pseudo Genes (internal stop)      :: 38 of 155
            Pseudo Genes (multiple problems)  :: 23 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022417.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="STW0522-39"
                     /isolation_source="sewage"
                     /db_xref="taxon:1463165"
                     /plasmid="pSTW0522-39-5"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            complement(102..623)
                     /locus_tag="JLF46_RS27440"
                     /old_locus_tag="STW0522KLE39_P50010"
     CDS             complement(102..623)
                     /locus_tag="JLF46_RS27440"
                     /old_locus_tag="STW0522KLE39_P50010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657475.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_099368844.1"
                     /translation="MDNSQIGTPESHEQDNYNFVQVSRAYLKEWRALTRKSPIASEILM
                     YLIENMGRTTNAVVCSYTTLMEITGVSRASVARAVKILKEDRWMQAVKIGNATAYCVNA
                     KAFWQAGRNQKKYAIFQATVIASSSEQESDFLEKSQQDLRYIPFVDLKDRPIVGTDELP
                     PPDQTDLDLD"
                     /name="replication/maintenance protein RepL"
     gene            1208..2176
                     /gene="mobV"
                     /locus_tag="JLF46_RS27445"
                     /old_locus_tag="STW0522KLE39_P50020"
     CDS             1208..2176
                     /gene="mobV"
                     /locus_tag="JLF46_RS27445"
                     /old_locus_tag="STW0522KLE39_P50020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_024227749.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNADAERTPENHCS
                     VSQSADEAMGKLRELLPEKRRKDAVLCVEYVMTASPEWWDKATPRQQAEFFARSEQWLE
                     NKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMRDDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSQAVNDGFAGTIAMASQSAQNAKRARDLQKTMDSQQKRLQSVTEPFKGLSREQM
                     THILTMAQTFQQQNHDREKQRQLEREQERQQRQKTDRGISR"
                     /name="mobV"
     gene            2173..2367
                     /locus_tag="JLF46_RS27450"
     CDS             2173..2367
                     /locus_tag="JLF46_RS27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_021567768.1"
                     /translation="MSEKQRREGFRKAEASLRLEGMDPSGVPRYECLKTRIISGETSYE
                     QGRKEILDYYLRINHKGEE"
                     /name="antitoxin VbhA family protein"
     gene            complement(2384..3094)
                     /locus_tag="JLF46_RS27455"
                     /old_locus_tag="STW0522KLE39_P50040"
     CDS             complement(2384..3094)
                     /locus_tag="JLF46_RS27455"
                     /old_locus_tag="STW0522KLE39_P50040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021567767.1"
                     /translation="MLYWKKMPSLWIGNRLTEFNSLETSHAIAAIKIYITFCLFGKEED
                     DGVRTVKLTFTEISKIASISRSLVNEGLKLLDNKKLIKYLSPASKRKKIYTVDVDGYIH
                     DGWCKLPMAGIVSEDRTISAFQSMHNRYHFELVALQLYLYLLYARDNFQEHVLARKQTI
                     CEKLACRNVDLNKAITYLIHIGLLKGIKQKAVESYPIELFHDSFYFYVKTGASNALTFK
                     KQPLVNPPEKEIPY"
                     /name="hypothetical protein"
     Typing          1161..2071
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.066"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 taacttttta taattaaatc aaaaagttaa tatcaaaaaa tggttatcaa atgaacttca
       61 atatggttca cggtgaacca tatttgaagg ttttcagagg ctcagtcgag atctagatct
      121 gtttgatccg gaggcgggag ttcatcggtg ccaacaatag ggcggtcttt gagatcgaca
      181 aaaggaatat atcgaagatc ttgctgtgat ttttctaaga aatcagactc ttgctctgag
      241 gaggaggcaa tcacggtagc ttggaagatt gcgtattttt tttgatttct acctgcctgc
      301 caaaatgctt tggcattaac acaatatgct gtggcattac ctatttttac tgcctgcatc
      361 catcgatctt ccttcaggat tttaaccgcc cttgctactg atgctctaga gaccccggtt
      421 atctccatca aagtcgtata gctgcaaaca acagcattag ttgtcctgcc catattctca
      481 atcaagtaca taaggatttc agaggcaata gggcttttcc ttgtcagcgc acgccattcc
      541 ttgagatacg cgcggctaac ctggacgaaa ttatagttat cctgctcatg agactctgga
      601 gtgccaatct gtgaattatc cattgctcta tagaccattt caatatccca tttgataccg
      661 gttgtatcat tagagatgca acaggtcaac ttgagtctca tagctagtga gactcataag
      721 tctcatggct ggtgagactc cccctttttt taacccaata taatcagcga tttactgaaa
      781 gctccttttc tatatctatt aagtccgtgc atacgtagcc caatgtacgc ctagtggaca
      841 ttccgcttcc tcgacatgct gttcactcct cgcttcgctg cggtgcgcct cagcatgtct
      901 tgcggtgagc ggtatttttt taaggactga cactggaact aatttcgtca agggcgagcg
      961 tagcgagtgc atttcaccct tgacgacgca caaataaaac aggcttggca gtggtgggag
     1021 gtggcgcgga gcgacgcctt ccacctgcca gaccgccgtt gattttgacc ttctggcagc
     1081 ttgctgcccg aacgattttg cgaagcaaag tctagtgagt acactatcat cctgatagtg
     1141 gcccgccgga ggcatcgcaa tgcgatccca cgactggctg gatgtacgcc agaacgaggg
     1201 ggtaagcatg gcgaattacg cgataatgcg ctgcaaaaag ctgacgggaa tgggcagtgt
     1261 ggccagcgct ttgcagcact gctacaggga gcgtgaaacg ccgaatgctg acgctgaacg
     1321 gacaccagaa aaccactgct ctgtgtctca gtcggcagat gaggcgatgg gaaagctgcg
     1381 ggagctgttg cctgaaaaac gccgtaaaga tgcggtgttg tgcgtcgagt acgtcatgac
     1441 ggcaagccct gagtggtggg acaaagctac accacggcag caggcggagt ttttcgcccg
     1501 ttctgagcaa tggctggaga acaaatacgg gaaagatcgc gttgtggcgg cggtggttca
     1561 cagggacgag gccaccccgc acctgtctgc gttcgtggtg ccattgacgc aggatgggcg
     1621 gctgtccgcg aaagagttta ttggcgggcg gtcaaagatg cgtgacgatc agagcaccta
     1681 cgctgaatcg gtaaaaaaac tgggtcttga gcgcggcatc gagggaagcc gtgccacgca
     1741 tcagacagtt cagcactact acgaatcgat aaaccgtggc acccgtagcc aggtatcgat
     1801 ctcaccggag gcgctggagc cgcgtgtact gcggaagggt attttcacca aagacgtgga
     1861 agatcaggca gccatagcca aacgtttaag ccaggcagtg aacgatggct ttgcaggaac
     1921 catcgctatg gcctctcaga gcgcccagaa tgccaaaaga gcgcgcgatc tacagaagac
     1981 tatggattcg cagcaaaaac gcctacagag cgttacagag ccttttaaag gactttcccg
     2041 ggagcagatg acccatattc tgacgatggc gcagacgttc cagcaacaga atcacgatcg
     2101 tgaaaaacag cgccagcttg aacgggagca ggaacgccag caacgtcaga aaactgacag
     2161 aggtattagc cgatgagtga aaagcagcgc cgtgaagggt ttcgaaaagc ggaagcatca
     2221 ctaagacttg agggaatgga tcctagtgga gtacctcgtt atgaatgttt gaaaacgagg
     2281 attatctctg gcgaaacaag ttatgaacaa gggcgtaaag aaatattgga ttactacctt
     2341 agaataaatc ataagggcga ggagtaattc gccctcaggt atgtcagtac ggaatttctt
     2401 tttctggagg gttaacaaga ggttgctttt tgaatgtaag cgcattgctt gctcctgttt
     2461 ttacataaaa ataaaatgaa tcatgaaaaa gttctatggg ataagactcc actgcctttt
     2521 gtttaattcc ttttaaaaga cctatgtgta ttaaatatgt tattgcttta tttaaatcta
     2581 cattgcggca agcaagcttt tcgcaaatag tttgttttct agctaacaca tgctcctgga
     2641 agttatctct ggcatataga aggtataaat atagctgaag cgctacaagc tcaaaatggt
     2701 atctattatg cattgactgg aaggcgctga ttgtcctgtc ttcgctaacg atgcctgcca
     2761 taggcagctt acaccatccg tcatgaatgt acccatcaac atccactgta tagattttct
     2821 ttctttttga cgcgggcgat aggtatttaa ttagtttttt attatcgagc aatttgagac
     2881 cttcatttac caaagatcgg ctgatagaag ctattttgct tatttctgta aacgtaagtt
     2941 ttacggttcg cactccatca tcctcttctt ttccaaagag acaaaaagtt atatatatct
     3001 ttatggcggc aatggcatgt gaagtctcta aactattaaa ctctgtcagt ctattaccga
     3061 tccagagtga tggcattttt ttccaatata acattagtga ctcctttatg cggaaatgaa
     3121 agtgaaataa atcataatta acacacaaat ggccaatgca agactagtaa gtgaccctgc
     3181 cacttttccc agcctttcag caagctctac aaatcgctct aagtcaccca ttttgacctc
     3241 tgctcctgtt taccgatata ccgattatcc cagtaatccg cgtatctgta tctacaggag
     3301 caaaagtcta tctagaaagg tatgggaaaa aattaata
//
