LOCUS       NZ_AP022414             7942 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain STW0522-39 plasmid pSTW0522-39-2,
            complete sequence.
ACCESSION   NZ_AP022414
VERSION     NZ_AP022414.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195879
            Sequence Read Archive: DRR199684
            Assembly: GCF_015135515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            STW0522-39 isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 288x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 10:03:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,526
            CDSs (total)                      :: 5,415
            Genes (coding)                    :: 5,260
            CDSs (with protein)               :: 5,260
            Genes (RNA)                       :: 111
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 63 of 155
            Pseudo Genes (incomplete)         :: 82 of 155
            Pseudo Genes (internal stop)      :: 38 of 155
            Pseudo Genes (multiple problems)  :: 23 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022414.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="STW0522-39"
                     /isolation_source="sewage"
                     /db_xref="taxon:1463165"
                     /plasmid="pSTW0522-39-2"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            102..293
                     /locus_tag="JLF46_RS27315"
                     /old_locus_tag="STW0522KLE39_P20010"
     CDS             102..293
                     /locus_tag="JLF46_RS27315"
                     /old_locus_tag="STW0522KLE39_P20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            293..694
                     /locus_tag="JLF46_RS27320"
                     /old_locus_tag="STW0522KLE39_P20020"
     CDS             293..694
                     /locus_tag="JLF46_RS27320"
                     /old_locus_tag="STW0522KLE39_P20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_048270303.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKTGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNILLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1752..1988)
                     /locus_tag="JLF46_RS27330"
     CDS             complement(1752..1988)
                     /locus_tag="JLF46_RS27330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_148399020.1"
                     /translation="MMRVLSLGAILMAMSALPLTAISQVTLASDGSYVSGSEATLAPDG
                     SYVGGSEVTLAPDGSYVGGSEATLAPDGSYVGN"
                     /name="hypothetical protein"
     gene            complement(2120..2311)
                     /locus_tag="JLF46_RS27335"
                     /old_locus_tag="STW0522KLE39_P20030"
     CDS             complement(2120..2311)
                     /locus_tag="JLF46_RS27335"
                     /old_locus_tag="STW0522KLE39_P20030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049106033.1"
                     /translation="MLKKDDIIFMKSCGNAKTIIFLRGVDEKLMINASFEEIKFAINNN
                     LQELPGVQAVAATLKIAT"
                     /name="hypothetical protein"
     gene            complement(2380..3500)
                     /locus_tag="JLF46_RS27340"
     CDS             complement(join(2380..3242,3242..3500))
                     /locus_tag="JLF46_RS27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611473.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISKpn34 family transposase"
                     /protein_id="WP_087759866.1"
                     /translation="MKKRFSDEQIICILREAEAGVSARELCRKHAISDATFYTWRKKYG
                     GMEVPEVKRLKSLEEENARLKKLLAEAMLDKEALQVALGRKLLTTDQKREAVEVMCEAK
                     GLSQRRACRLAGLSLSTCRYSAQRPAADAQLSLRITELALERRRFGYRRIWQLLRREGL
                     CVNHKRVYRIYQLNGLSVKRRRRRKGLATERLPLLRPMAPNLTWSMDFVMDALATGRRI
                     KCLTCVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPAMIRTDQGPEFTCRALDQ
                     WAFEHGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIINDWRLDYNE
                     CRPHSSLNYLTPAEFAAGWRNGKYEEKPTDITN"
                     /name="IS3-like element ISKpn34 family transposase"
     gene            complement(3910..4023)
                     /locus_tag="JLF46_RS27850"
                     /pseudo=""
     CDS             complement(3910..4023)
                     /locus_tag="JLF46_RS27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            4439..4686
                     /locus_tag="JLF46_RS27855"
                     /old_locus_tag="STW0522KLE39_P20060"
                     /pseudo=""
     CDS             4439..4686
                     /locus_tag="JLF46_RS27855"
                     /old_locus_tag="STW0522KLE39_P20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4686..5108
                     /locus_tag="JLF46_RS27355"
     CDS             4686..5108
                     /locus_tag="JLF46_RS27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5856..>6197)
                     /locus_tag="JLF46_RS27360"
                     /old_locus_tag="STW0522KLE39_P20070"
                     /pseudo=""
     CDS             complement(5856..>6197)
                     /locus_tag="JLF46_RS27360"
                     /old_locus_tag="STW0522KLE39_P20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <6196..6798
                     /locus_tag="JLF46_RS27365"
                     /pseudo=""
     CDS             <6196..6798
                     /locus_tag="JLF46_RS27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="klebicin B-related nuclease bacteriocin"
                     /name="klebicin B-related nuclease bacteriocin"
     gene            6800..7057
                     /locus_tag="JLF46_RS27370"
     CDS             6800..7057
                     /locus_tag="JLF46_RS27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            7165..7320
                     /locus_tag="JLF46_RS27375"
     CDS             7165..7320
                     /locus_tag="JLF46_RS27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(7478..7639)
                     /locus_tag="JLF46_RS27860"
     CDS             complement(7478..7639)
                     /locus_tag="JLF46_RS27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049165871.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     Typing          complement(3911..4318)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.377"
                     /coverage="99.0"
                     /evalue="1.26e-177"
     Typing          complement(7527..7903)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.503"
                     /coverage="99.0"
                     /evalue="7.66e-170"
ORIGIN
        1 ccgctcgccg cagccgaacg gcaggagcgt agcgactgag tgagcgagga agcggaaaag
       61 agatctgtct gacattgagc actgacgctc tgaggccctt tatgacgaaa caacagaaaa
      121 cagcgctgaa catggctaaa ttcatccaga atcagtctct gctcctgctg gagaagctta
      181 acgagcttga tctcgatgtt gaggccgacc tttgtgagaa actccacgat gatgcggagc
      241 atcttttccg tacactgtcc tcgagactgg atgcattaca ggatggtaac tgatggccag
      301 agtgtctatt agtgaggctg cccgactgac gggcaagagt agaacaacac tgcacaggtt
      361 aattaaaaca ggtgacctgt ccacctgtag tggtgagcgc aatgcaaaaa tggtagatac
      421 ttcagagctt ttacgtgtct ttggtccctt tgagcaaccg aagggtgaac accattctgg
      481 acaggtctct gaacagcgtg atacagcctc tgctgaccag agtgaacagg tgattcgtca
      541 tctgaaacag gagattgaac acctgaatat cttacttttg tccaaggatt cgcacattga
      601 cagcctgaaa caggccatgc tccttcttga aaatcaccag gcaaaattaa ctgaatcccc
      661 ggcgccatgg tggaaattct ggaaaaaatc ctgaccccgt catgcttccg ttttcctctc
      721 cggccatccc ggggagttcc tgaaccttcc aaaaaatcgc cggatttctg aacgctcctc
      781 accactaaaa atacccacac actacgcaaa attaaaattt tgcagattcc ttatttacaa
      841 caatgggtta atgaaaaata aatgtgcggt gatgtgttac aaagtgcaga tccaagtcgt
      901 ggacaactct atggcgaatc gctgacgaat tgcccacgat tcatatgtga atcacgcttt
      961 attatcatat tgttatgatg aatcgactct tgattcaatt catcactctg ggtttttttt
     1021 gttcatggtg actcttgatt caattcacga ttcgaatcgc cggtgaatcg tgtggtgagt
     1081 cgtccggcga cttgccataa cgtgattgcc ggccgtcgac gaacccgatt cctgaacgtg
     1141 ctgaaaaccg gttttccggc tgcgccggca acgcttcacg gccacgcaat aaattgcgtc
     1201 cccgttcacc gttttcggat aggaagaacg ccggaaacag gacttaccct ggctgaatgg
     1261 cgtgaatttt cgcgctatgc acttgcgtgc atactcatgc atgccgtaaa aacagagcct
     1321 gcgcgtttct ggcgggtttt cgggtggttt gttgcctgtt ttaccggttt cccgtcagaa
     1381 acgccttgag gccgtttttg cggtgcgtac aagaagggat tatggtaaat agggcgctat
     1441 cgcctaaatg tacgactgat tgtttttttt tctttgtagt atgagattcc tgagtagaca
     1501 gctacaacaa gtagagcaat ggcgatagca agtaaaaaaa agtccataag tactttatta
     1561 atctaataaa aacaacctgt tatgattagt tattgtaagt ggttttcatt cttttttcat
     1621 tacacttatt gctttgagct aatggttcct gaggacgcat tgttcagtac acgtaagaag
     1681 acgttatgat aaatccaaaa atgctggcat tcggaattca gtatatcggt acgcagcata
     1741 ataatggggg gttaatttcc aacgtatgag ccatcaggtg ctaaggttgc ttccgaacca
     1801 ccaacgtatg agccatcagg tgctaaggtt acttccgaac caccaacgta tgagccatca
     1861 ggtgctaagg ttgcttccga accactaacg tacgatccat cagatgctaa ggttacttgg
     1921 gatatcgcgg ttagtggtaa tgctgacata gccattaaaa tggcaccaag agataatact
     1981 ctcatcacta ttactcctgt taatatcaaa aatagaattg aatcgttcag cggtgccgaa
     2041 cgccggttcc agccgcggga ttcgtgatcg ggatgttata gtaaattgat ctcttgattt
     2101 tgagtgccct tactcgaaat caggttgcta ttttcagtgt cgcggctact gcttgtaccc
     2161 ccggaagctc ctgaagatta ttattgatag caaactttat ctcttcaaag ctcgcgttaa
     2221 tcatgagctt ctcatccacg ccacgtaaaa atattattgt ttttgcatta ccacatgatt
     2281 tcataaagat aatatcatct ttttttagca tgatactgtc gttacttagt gtagtgagat
     2341 aaagcatagc tgacctgccc ccagagttag atacaacctt cagttagtaa tgtcggttgg
     2401 tttttcttca tatttcccgt ttcgccagcc cgctgcaaat tcagccggcg tcaggtaatt
     2461 cagtgatgaa tgtggtcgac actcgttata atccagtcgc cagtcattaa tgatcttcct
     2521 ggcgtgaaca atatcgctga accagtgctc attcaggcat tcatcacgaa agcgtccgtt
     2581 aaaactctca ataaatccgt tctgcgttgg cttgccgggc tggataagtc gcagctccac
     2641 accatgctca aaagcccact gatcgagtgc gcggcaggta aactccgggc cctgatcggt
     2701 tcttatcata gccggatagc cgcgaaacag cgcaatgctg tccagaatac gcgtgacctg
     2761 cacgcctgaa atcccgaagg caacagtgac cgtcaggcat tcctttgtga aatcatccac
     2821 gcaggtcagg cacttgatcc tgcgacctgt ggccagtgcg tccatgacga aatccattga
     2881 ccaggtcaga ttgggcgcca tcgggcggag cagcggcaga cgttctgttg ccagcccttt
     2941 acgacgtcgt ctgcgtttta cactcaggcc attaagttga tagatgcggt aaacccgctt
     3001 gtggttaacg caaagacctt cacgtcgcag aagctgccag atacgccggt aaccaaaacg
     3061 gcggcgttca agtgccagct ctgtgatgcg tagagacagc tgcgcgtcag cagccggacg
     3121 ctgagccgaa tatcggcagg ttgacaggga cagacctgcc agcctgcagg cacgacgttg
     3181 cgacagaccc ttagcctcgc acatgacttc cacggcttcc cgcttctggt ctgtcgtcag
     3241 tactttcgcc caagagccac ctgaagcgcc tccttatcca gcatggcttc agcaagcagc
     3301 ttcttgagtc tggcgttctc ttcctcaagc gacttcaggc gcttaacctc aggcacctcc
     3361 ataccgccat acttcttacg ccatgtgtaa aaggtggcgt cggaaatggc gtgcttacgg
     3421 cagagctcac gggcagaaac gccggcttcg gcctcgcgga gaatacatat gatctgttcg
     3481 tcggaaaaac gcttcttcat ggggatgtcc tcatgtggct tatgaagaca ttactaacat
     3541 cacggtgtat taatcaacgg ggagcaggtc aaccgcgttg gatgattgtg gatcatgatt
     3601 atatatgttt ttttcttatc agaaacaatg ggttaaatag ttttattttg atggttggtg
     3661 tcgcggagtg gtgctggttt cacttcaggg gtaggcatat ggaacaggat cctcttgaga
     3721 tcctttcttt agcatcgtaa tctcttgctc tgtaaacgaa aaaaccgccc agcaaggcgg
     3781 ttttctcgaa ggttctcaga gcaacaactc tttgaaccga ggtaactggc ttaaagggag
     3841 cgcagtcacc aaaactgttc gtttagttta gccctaactg gcgtataact tcaagactaa
     3901 ctccctaaat cagttaccag tggctgctgc cagtggcgct ttgtcgtgtc tttccgggtt
     3961 ggactcaaga cgatagttac cggattaggc gcagcagtcg gactgaacgg ggggttcgtg
     4021 catacagtcc agcttggagc gaactgcctt cccggaactg agtgtcaggc gtggaatgag
     4081 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
     4141 gcacgaggga gccatcaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4201 accactgatt tgagcgtcag attctgtgat gcttgtcagg ggggcggagc ctatggaaaa
     4261 aaggccacca tgcggccttt ccttttcacc atcacgcgac tgttcggttt tctcgagctt
     4321 ttatgtgcat gctcctgccg ctcgccgcag ccgaacgacc ggagcgtagc gactgagtga
     4381 gcgaggaagc ggaaaagaga acatccctac actgagcact gacgctctga ggcctcttat
     4441 gacgaaacaa cagaaaacag cgctgaacat ggctaaattc atccagaatc agtctctgct
     4501 cctgctggag aagcttaacg agcttgatct cgatgtcgac agaatcagtc tctgctcctg
     4561 ctggagaagc ttaacgagct tgatctcgat gtcgaggccg acctttgtga ggaactccac
     4621 gatgatgcgg agcatctttt ccgtacactg tcctcgagac tggatgaatt acaggatggc
     4681 aattgatggc cagagtgtct attagtgaag cggcacggct tgttaaggtt agccgtccca
     4741 caatttacaa gatgattaac agtgggaagt taagttacac ctctgttgta aagcacggga
     4801 aggctattaa agttattgat acttcagagc ttattagagt ttttggttct cttgatggtg
     4861 tcatcgatac tgtaaaatat gatgtaaaaa gtgacgcaga atctacaagt attaacagcg
     4921 ttggtttaca tgatttacaa catagaatcg ctctacttga agctgaaaac gatggtctta
     4981 aaggggctgt taaggctcga gatgagcata ttgattccct tcgtcaggct atgcagcttc
     5041 tggagcacaa gcgcgagcct tcttcaccac cccattcccc gtggtggaaa ttctggaaaa
     5101 aatcctgatc ctgccatgct tgagttttcc tctccggcca tcccagggag ttcctgaacc
     5161 ttcccaaaaa tcgccagatc tctgaacgct cctcacgact aaaaataccc acacactacg
     5221 caaaattaaa attttgcaga ttccttattt aaaacaattg gttaatgaaa aattaatgtg
     5281 cagtgatgtg ttacaaagtg cagatccaag tcgtggacga ctctatggcg aatcgtttac
     5341 gaattgccca cgattcatat gtgaatcacg ctttattatc atgttgttat gatgaatcga
     5401 ctcttgattc aattcatcaa tttgggcgtt ttttgttcat ggtgactctt gattcaattc
     5461 acgattcgaa tcgccggtga atcgtgtggt gagtcgtccg gcgacttgcc ataacgtgat
     5521 tgccggccgt cgacgaaccc gattcctgaa cgtgctgaaa accggttttc cggctgcgcc
     5581 ggcaacgcct cacggccacg caataaattg cgtccccgtt caccgttttc ggatcagaag
     5641 aacgccggaa acaggactta ccctggctga atggcgtgaa tttccgcgct atgcacttgc
     5701 gcgcatactc atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg
     5761 gtttgttgcc tgttttaccg gtttcccgtc agaaacgtcc tgaggccgtt ttagcggtgc
     5821 gtacaattaa gggattatgg taaatggtga cttggttaat cataaacatc ctgggggatg
     5881 gcatacacca gttggatatc cggtgcatcg ttgagccatc gacctgattt tttcattaag
     5941 tagccaccaa tttcttcggt agtcagccgg gcatgaaaca atgcttcatc attgaaccag
     6001 cagtcagggt gatagatgag atccagtggg tttgccttga aattaatctc cagtaactgg
     6061 agtgcatagc tgtggtcaga tgcactacct tctgctttgc gtatgaagtt gataacgtcg
     6121 ttcagttctt tccatgtaaa gtcatcgagg tatttttcct gattaagcgc catcctggtg
     6181 aaatctagtg cacttgacgc acctggtgca aacggaccac tgacgctaag cgggccggta
     6241 ccactaccgg agcgtattct gcataccggt gatcagattt cggcgccaca ggctacagat
     6301 aaaaccgtga cgccggtagc ggatgacctc gattttgacg atattattct ggtgtttcct
     6361 ccggaatccg gcctaaaacc gctgtatgtg atgtaccgta gccctcgtaa catgcccggt
     6421 acagtgagcg gcaaagggca gaacgttggc aataactgga tggggggggc cagtaccggg
     6481 gatggtgctc ctgttccttc ccagattgcg gataaattac gcgggaagac tttcggtagt
     6541 tttgattctt tccgtcgggc gttctggaaa gcggttgctg acgattctgc actgagcaag
     6601 caatttagtg aagctgatat taatcagatg aaagctggaa gggcacctac tgcggacttt
     6661 ttagaatcag tagggaaacg tgttaaaatt gaattacacc acgaaaaaga aatatctcaa
     6721 ggaggggctg ttatggatgt cgataacatt aaggcgctaa cacctaaaaa tcatattgag
     6781 acacataaag gaaaataaaa tggctggtaa taaaatttca gattttacgg aagaagaatt
     6841 tactgctttt gttagtaaaa taatcgctgc tgattttcca actgacaaag agaacgatga
     6901 tgctatttat tcgttcgccc aactaacaga gcatcctgct ggttggaacc tcatcttcaa
     6961 gcctaaggta ggcgaagatt cttcggcgaa aggaattgtt aaaaccgtaa aggaatggcg
     7021 agctgctaac ggtaagccag gttttaaaaa agcgtgattg aatttgcggg ttcaagttat
     7081 gagcccgctc atttgtaact ctggatttga agaataatta ctagatatcc atttgcctag
     7141 aataataaaa ataaaaggtg atatatgaaa aaattacttt tcctaggggt tgttaccgtt
     7201 tctgtggttc tggcagcatg tcaggtcaat aatgttcgag acaccggagg tggggctgtt
     7261 tcaccttcat cgacggtgac cggagtcacg cttgatagcg gtacgaacgg caatccgtaa
     7321 aggatcttct tgagatccca tttggatcgt cgtaatctct tgctctgtaa acgaaaaaac
     7381 cgccttgcag ggcggttttt ccgaaggttc aaacacttgc aggtgattga accaaggtaa
     7441 ctggcttaga ggagcgcagt caccaaaaat tgttctttca gtttagcctt aacaggcgca
     7501 tgacttcaag accaactcct ctaaatccgt taccagtggc tgctgccagt ggcgctttgt
     7561 cgtgtctttc cgggttggac tcaagatgat agttaccgga taagacgcag cagtcggact
     7621 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg
     7681 tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaatg
     7741 gcaggaacag gagagcgcac gagggagcca tcagggggaa acgcctggta tctttatagt
     7801 cctgtcgggt ttcgccacca ctgatttgag cgtcaaattc tgtgatgctt gtcagggggg
     7861 cggagcctat ggaaaaacgg ccgctgtgct gccttctccc tctccgagct gtctgctcta
     7921 tgtcgttatg tgcgcgttgc ga
//
