LOCUS       NZ_AP022408             2266 bp    DNA     circular CON 30-JAN-2024
DEFINITION  Citrobacter portucalensis strain STW0522-30 plasmid pSTW0522-30-9,
            complete sequence.
ACCESSION   NZ_AP022408
VERSION     NZ_AP022408.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00195870
            Sequence Read Archive: DRR199675
            Assembly: GCF_015135215.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter portucalensis
  ORGANISM  Citrobacter portucalensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Citrobacter portucalensis str.
            STW0522-30 isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 387x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 09:04:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,249
            CDSs (total)                      :: 5,135
            Genes (coding)                    :: 4,958
            CDSs (with protein)               :: 4,958
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 35 of 177
            Pseudo Genes (incomplete)         :: 134 of 177
            Pseudo Genes (internal stop)      :: 29 of 177
            Pseudo Genes (multiple problems)  :: 19 of 177
            Pseudo Genes (short protein)      :: 1 of 177
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022408.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Citrobacter portucalensis"
                     /mol_type="genomic DNA"
                     /strain="STW0522-30"
                     /isolation_source="sewage"
                     /db_xref="taxon:1639133"
                     /plasmid="pSTW0522-30-9"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            complement(102..401)
                     /locus_tag="JLF43_RS26080"
                     /old_locus_tag="STW0522CIT30_P90010"
     CDS             complement(102..401)
                     /locus_tag="JLF43_RS26080"
                     /old_locus_tag="STW0522CIT30_P90010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_097455297.1"
                     /translation="MEQKTNKKMEKIITETLHSLGLKTTKESLNKTIFLLNFGSLKSHQ
                     AVFDEAFNEIAEAKQRRSWQVITECLNENTNTKMTVMTVRTMFKRAKEKRQADK"
                     /name="hypothetical protein"
     gene            1039..1251
                     /locus_tag="JLF43_RS26085"
                     /old_locus_tag="STW0522CIT30_P90020"
     CDS             1039..1251
                     /locus_tag="JLF43_RS26085"
                     /old_locus_tag="STW0522CIT30_P90020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_089634954.1"
                     /translation="MSNIKPDSLIRITKRKNENGDETTDLEWKPGSKEAIATGAAAGAA
                     LGSAVPVIGTAVGAVIGGTLALIFG"
                     /name="hypothetical protein"
     gene            complement(1340..1762)
                     /locus_tag="JLF43_RS26090"
                     /old_locus_tag="STW0522CIT30_P90030"
     CDS             complement(1340..1762)
                     /locus_tag="JLF43_RS26090"
                     /old_locus_tag="STW0522CIT30_P90030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_097455296.1"
                     /translation="MKLKFIPIFLFIVTLQAAHSSGIDSTPPPQGKVIYTMNCTFVDDT
                     GKAIGKSQKYILRKGGDGREQMEITPQENAHVPPPIYIQKTSGAYTNGGFKTFYWNNTV
                     RMTVIRGDKTTVEIEFIDSPAISIAKATCDNPVYLP"
                     /name="hypothetical protein"
ORIGIN
        1 aaagcccgca acgctcgccg cagtcgaacg accgaacggc gtgagcgagt gagcgaggaa
       61 gcgtaatgta acctccgttt gtggcgtctc gttacgcacc attacttgtc tgcctgcctt
      121 ttttcctttg ctcttttgaa catggttctg acggtcatta ccgtcatttt tgtattcgta
      181 ttttcgttca ggcactcagt gatcacttgc cagcttctcc gctgtttggc ttcggctatc
      241 tcattaaatg cctcatcaaa taccgcttga tggctcttta gcgaaccgaa attcagcaga
      301 aagatcgttt tgttcaacga ttcttttgtc gttttcagac cgaggctatg tagcgtttcc
      361 gtgattatct tttccatttt tttgtttgtt ttttgctcca tctagttcat gtgtacgcaa
      421 tgttgtacgt cgttttgtag gcatatttcg tggatgtgta cgttgttttg tacggaacca
      481 ttcggttcat gtacgcagat ttgtacgcag gacaaaaatg aatctagagc tacaggcacg
      541 agggcgaccg tatacaattg aggacaaaat gaggacattt tatcgcaagt gtatacggtc
      601 gtgtgccaaa gggaagttcc ctttgtaacc ctgataaggt tctacgaacc tcaaaaccct
      661 accgcctcgt tttactcgtt ggtcaacgtt tcactgccgg ataaaaaatt tatccgcccg
      721 ttcaccgttt caggtgtggg gttcggatgt ggttttgcgt gtttcgtggg gattattcac
      781 tgagatcggc ggtgggtcag tccggtgcat aacgattcat gcccgtaaaa cacatcagcg
      841 ccattctgag gcgttttttg gggtgaaggt gtgtggtttt gtggctcagg cgggaaaatc
      901 gcgcacaggc cgtttcagcg gtgcgcgcaa tgagacgtta tggtaaatgc agggttttta
      961 atatatgtag ttgaaatgta gcctaggtgg agggagagtg tttttggtgt atacgtttca
     1021 atcttacgtg aggtgcgaat gagcaatatt aaacctgata gtctgatccg gattacaaaa
     1081 agaaagaatg aaaatggcga tgaaacaaca gatttagaat ggaagcctgg ttcaaaagag
     1141 gctattgcca caggcgcggc tgctggagca gcactaggtt ctgctgttcc tgtaatagga
     1201 actgcagtcg gggctgtgat cggtgggact ttagctttaa tttttggata accaagttta
     1261 gagcgaaaca cccgtcaaga atggcggaag gtagcctgct aaaagcgggc ttttttacgc
     1321 tcatgcgagc aatgacgcgt tatggtaaat aaacaggatt gtcgcatgtg gctttcgcta
     1381 tcgaaatcgc aggggaatca ataaactcta tttccacagt ggttttatcg cccctgatca
     1441 cggtcatccg tactgtattg ttccagtaga aggttttgaa cccaccattg gtgtaagcac
     1501 cacttgtttt ttgtatgtat atgggtgggg ggacatgtgc gttttcttgt ggagttatct
     1561 ccatttgctc acgaccatcc cccccctttc gcaggatgta tttttgcgac ttaccaatag
     1621 ccttaccggt atcatcgaca aaagtgcaat tcattgtgta tatcaccttc ccttgagggg
     1681 ggggcgtgct atcaattcca ctggaatgag cggcttgtaa cgtaacgata aataaaaaaa
     1741 taggtatgaa ttttagcttc acgtagtgtc tccgttgcaa aaaaagccta atgcccccgt
     1801 ggtgggtata taaaaatcac ggggagcaga gcgaccttcg tccagctcgt ttcttgtgtc
     1861 tctcaccctg caaggggctt gattgtgtgg gtgaagtgtc agaccttagc aatcacactt
     1921 caccgcttac cgctgccttg ttcgctcatt ttatcaggtg ccagttatcg tactgcgaga
     1981 cggggtagag cttaactctt tcgcggttaa gttacgttcc caccgtttgc ctgtttatcc
     2041 tgccggagtc cggggcgtag acctgagcgt ctaaggtgca gatatgattt taatgtcgtt
     2101 gaatggtatt aagctggtag tgacaaggct agccagccct gctaggattg gattgcaacg
     2161 tctattcctg gcaggtatct gcgaagaatt agagtaccga agccctgtga ttctgtcacg
     2221 gggcttttgt acatttagat ttcataaatc aaaaacatcc tctcag
//
